scholarly journals Partial rpoB Gene Sequencing Identification and Probiotic Potential of Floricoccus penangensis ML061-4 Isolated from Assam Tea (Camellia sinensis var. assamica)

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Patthanasak Rungsirivanich ◽  
Angkhana Inta ◽  
Yingmanee Tragoolpua ◽  
Narumol Thongwai
Keyword(s):  
Camellia Sinensis ◽  
Competitive Inhibition ◽  
Lactic Acid Production ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Raw Material ◽  
Bacterial Isolates ◽  
Northern Thailand ◽  
E Coli ◽  
Assam Tea

Abstract Assam tea or Miang is a local name of Camellia sinensis var. assamica in northern Thailand. By the local wisdom, Assam tea leaves are used as the raw material in tea fermentation to produce “Fermented Miang” consumed by people in northern Thailand and the countries nearby. In this study, twenty-eight bacterial isolates were obtained from Assam tea leaf samples collected from Nan province, Thailand. Bacterial isolates were identified within 6 genera including Bacillus, Floricoccus, Kocuria, Lysinibacillus, Micrococcus and Staphylococcus. Among these, the strain ML061-4 shared 100.0 and 99.4% similarity of 16S rRNA and rpoB gene sequence with F. penangensis JCM 31735T, respectively. This is the first discovery of F. penangensis in Thailand. F. penangensis ML061-4 exhibited probiotic characteristics including lactic acid production (9.19 ± 0.10 mg/ml), antibacterial activities (Escherichia coli ATCC 25922 and E. coli O157:H7 DMST 12743), acid and bile salt tolerance (71.1 and 54.9%, respectively), autoaggregation (97.0%), coaggregation (66.0% with E. coli O157:H7), cell surface hydrophobicity (90.0%), bacterial adhesion (82.9% with Lactobacillus plantarum FM03-1), competitive inhibition (17.8% with E. coli O157:H7) and competitive exclusion (34.9% with E. coli O157:H7). Overall, the data suggested that F. penangensis ML061-4 had a great potential to be a probiotic.

scholarly journals Culturable Bacterial Community on Leaves of Assam Tea (Camellia sinensis var. assamica) in Thailand and Human Probiotic Potential of Isolated Bacillus spp.

2020 ◽  
Vol 8 (10) ◽  
pp. 1585 ◽  
Author(s):  
Patthanasak Rungsirivanich ◽  
Witsanu Supandee ◽  
Wirapong Futui ◽  
Vipanee Chumsai-Na-Ayudhya ◽  
Chaowarin Yodsombat ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Camellia Sinensis ◽  
Antibacterial Properties ◽  
Cell Surface Hydrophobicity ◽  
Vero Cells ◽  
Surface Hydrophobicity ◽  
Inhibitory Effect ◽  
Northern Thailand ◽  
Bacillus Spp ◽  
Assam Tea

Assam tea plants (Camellia sinensis var. assamica) or Miang are found in plantations and forests of Northern Thailand. Leaf fermentation has been performed for centuries, but little information is available about their associated microbial community. One hundred and fifty-seven bacterial isolates were isolated from 62 Assam tea leaf samples collected from 6 provinces of Northern Thailand and classified within the phyla of Firmicutes, Actinobacteria, and Proteobacteria. Phayao and Phrae provinces exhibited the highest and the lowest bacterial diversities, respectively. The bacterial community structural pattern demonstrated significant differences between the west and the east sides. Since some Bacillus spp. have been reported to be involved in fermented Miang, Bacillus spp. isolated in this study were chosen for further elucidation. Bacillus siamensis ML122-2 exhibited a growth inhibitory effect against Staphylococcus aureus ATCC 25923 and MRSA DMST 20625, and the highest survival ability in simulated gastric and intestinal fluids (32.3 and 99.7%, respectively), autoaggregation (93.2%), cell surface hydrophobicity (50.0%), and bacterial adherence with Vero cells (75.8% of the control Lactiplantibacillusplantarum FM03-1). This B. siamensis ML122-2 is a promising probiotic to be used in the food industry and seems to have potential antibacterial properties relevant for the treatment of antibiotic-resistant infections.

scholarly journals Cell surface hydrophobicity, adherence to HeLa cell cultures and haemagglutination pattern of pyelonephritogenicEscherichia colistrains

1990 ◽  
Vol 105 (2) ◽  
pp. 255-263 ◽  
Author(s):  
A. Brauner ◽  
M. Katouli ◽  
K. Tullus ◽  
S. H. Jacobson
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Hela Cell ◽  
Cell Cultures ◽  
Hela Cells ◽  
Acute Pyelonephritis ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Hydrophobic Properties ◽  
E Coli

SUMMARYCell surface hydrophobicity, haemagglutination pattern and adherence to HeLa cells were examined in 230 strains ofEscherichia colicollected from women (n= 61 strains) and children (n= 65 strains) with non-obstructive acute pyelonephritis and in 104 faecal control strains ofE. colifrom healthy adults (n= 71 strains) and children (n= 33 strains). PyelonephritogenicE. colistrains showed a significantly increased incidence of hydrophobic properties (90%) and mannose resistant haemagglutination (MRHA) of human erythrocytes (83%) than faecal control strains (64 and 23% respectively,P< 0·001 in both cases). Mannose sensitive haemagglutination (MSHA) was observed in 48% of the pyelonephritogenicE. colistrains and in 50% of the faecal control strains (NS). The incidence of adherence to HeLa cells was low both in pyelonephritogenic and faecal control strains, 6 and 7% respectively (NS). The bacterial phenotypes MRHA + MSHA + and MRHA + MSHA− appeared significantly more often in pyelonephritogenicE. colistrains (35 and 48% respectively) than in faecal control strains (5 and 17% respectively,P< 0·001 in both cases). The phenotype MRHA − MSHA + occurred significantly more often in control strains (45%) than in pyelonephritogenic strains (13%,P< 0·001). Eighty-three per cent of the pyelonephritogenicE. colistrains expressing hydrophobic properties showed MRHA and 50% of the hydrophobic strains showed MSHA. There were no significant correlations between cell surface hydrophobic properties and haemagglutination pattern or adherence to HeLa cells in pyelonephritogenicE. colistrains nor in faecal control strains.

Antibiofilm Activity of Crude Cell Free Extract from Bacillus subtilis S01 against E. coli

2018 ◽  
Vol 10 (2) ◽  
pp. 211-221 ◽  
Author(s):  
S. M. A. Sayem ◽  
A. J. M. T. Chowdhury ◽  
M. Z. Alam ◽  
P. K. Sarker
Keyword(s):  
Bacillus Subtilis ◽  
Cell Surface ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Microtiter Plate ◽  
Antibiofilm Activity ◽  
Potential Candidate ◽  
Growth Curve Analysis ◽  
E Coli ◽  
Crude Extracts

Antibiofilm phenomenon has become a novel area of research for removing deleterious biofilm. In the present study, strains from different environmental sources were tested for screening antibiofilm compounds. Crude extracts from various microorganisms were evaluated for antibiofilm phenomenon through crystal violet assay and growth curve analysis. Characterization of antibiofilm compound was performed by pre-coating microtiter plate and Cell Surface Hydrophobicity experiment. Among the organisms, cell free extracts (5% v/v) from Bacillus subtilis S01 inhibited the development of E. coli PHL628 biofilm by 63%. The cell free extracts possessed no amylase activity and had no effect on the planktonic growth of biofilm forming bacteria. Moreover, no competition with quorum sensing analogues was found with the extract. Biofilm formation was more inhibited (76%) in the B. subtilis S01 extract pre-coated wells than uncoated wells (62%). However, no effect on preformed biofilm was observed with the extracts of B. subtilis S01. The extract also reduced the cell surface hydrophobicity (69%) of the biofilm forming bacteria. The present study indicated that the crude extracts of B. subtilis S01 from soil origin has anti-adherence properties towards biotic and abiotic surfaces and thus can be a potential candidate in preventing the development of biofilm.

Medicinal Plant Extracts as Anti–Escherichia coli O157:H7 Agents and Their Effects on Bacterial Cell Aggregation

2006 ◽  
Vol 69 (10) ◽  
pp. 2336-2341 ◽  
Author(s):  
SUPAYANG PIYAWAN VORAVUTHIKUNCHAI ◽  
SURASAK LIMSUWAN
Keyword(s):  
Escherichia Coli ◽  
Medicinal Plants ◽  
Cell Surface ◽  
Cell Surface Hydrophobicity ◽  
Ethanolic Extract ◽  
Surface Hydrophobicity ◽  
Enterohemorrhagic Escherichia Coli ◽  
E Coli ◽  
Ethanolic Extracts ◽  
Bactericidal Activities

Ethanolic extracts of eight Thai medicinal plants (representing five families) that are used as traditional remedies for treating diarrhea were examined with a salt aggregation test for their ability to modulate cell surface hydrophobicity of enterohemorrhagic Escherichia coli strains, including E. coli O157:H7. Four of these medicinal plants, Acacia catechu, Peltophorum pterocarpum, Punica granatum, and Quercus infectoria, have high bacteriostatic and bactericidal activities. The ethanolic extract of Q. infectoria was the most effective against all strains of E. coli, with MICs of 0.12 to 0.98 mg/ml and MBCs of 0.98 to 3.91 mg/ml. The ethanolic extract of P. granatum had MICs of 0.49 to 1.95 mg/ml and MBCs of 1.95 to 3.91 mg/ml. Ethanolic extracts of Q. infectoria, P. pterocarpum, and P. granatum were among the most effective extracts against the two strains of E. coli O157:H7. The other four plants, Andrographis paniculata, Pluchia indica, Tamarindus indica, and Walsura robusta, did not have high bacteriostatic and bactericidal activities but were able to affect hydrophobicity characteristics on their outermost surface. All plants except Q. infectoria had some ability to increase cell surface hydrophobicity. There appears to be no correlation between antibacterial activity and cell aggregative properties.

scholarly journals A previously uncharacterized gene, yjfO (bsmA), influences Escherichia coli biofilm formation and stress response

Microbiology ◽  
2010 ◽  
Vol 156 (1) ◽  
pp. 139-147 ◽  
Author(s):  
Mary M. Weber ◽  
Christa L. French ◽  
Mary B. Barnes ◽  
Deborah A. Siegele ◽  
Robert J. C. McLean
Keyword(s):  
Escherichia Coli ◽  
Stress Response ◽  
Mutant Strain ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Insertion Mutagenesis ◽  
Flagellar Motility ◽  
Biofilm Growth ◽  
E Coli ◽  
Uncharacterized Gene

Bacteria growing as surface-adherent biofilms are better able to withstand chemical and physical stresses than their unattached, planktonic counterparts. Using transcriptional profiling and quantitative PCR, we observed a previously uncharacterized gene, yjfO to be upregulated during Escherichia coli MG1655 biofilm growth in a chemostat on serine-limited defined medium. A yjfO mutant, developed through targeted-insertion mutagenesis, and a yjfO-complemented strain, were obtained for further characterization. While bacterial surface colonization levels (c.f.u. cm−2) were similar in all three strains, the mutant strain exhibited reduced microcolony formation when observed in flow cells, and greatly enhanced flagellar motility on soft (0.3 %) agar. Complementation of yjfO restored microcolony formation and flagellar motility to wild-type levels. Cell surface hydrophobicity and twitching motility were unaffected by the presence or absence of yjfO. In contrast to the parent strain, biofilms from the mutant strain were less able to resist acid and peroxide stresses. yjfO had no significant effect on E. coli biofilm susceptibility to alkali or heat stress. Planktonic cultures from all three strains showed similar responses to these stresses. Regardless of the presence of yjfO, planktonic E. coli withstood alkali stress better than biofilm populations. Complementation of yjfO restored viability following exposure to peroxide stress, but did not restore acid resistance. Based on its influence on biofilm maturation and stress response, and effects on motility, we propose renaming the uncharacterized gene, yjfO, as bsmA (biofilm stress and motility).

scholarly journals Molecular identification and in vitro evaluation of probiotic functional properties of some Egyptian lactic acid bacteria and yeasts

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Al-Shimaa Ibrahim Ahmed ◽  
Gihan Mohamed El Moghazy ◽  
Tarek Ragab Elsayed ◽  
Hanan Abdel Latif Goda ◽  
Galal Mahmoud Khalafalla
Keyword(s):  
Lactic Acid ◽  
Cell Surface Hydrophobicity ◽  
16S Rrna Gene Sequencing ◽  
Surface Hydrophobicity ◽  
Rrna Gene ◽  
E Coli ◽  
Probiotic Potential ◽  
Health Promoting ◽  
Rrna Gene Sequencing

Abstract Background The health-promoting effects along with global economic importance of consuming food products supplemented with probiotic microorganisms encouraged the researchers to discover new probiotics. Results Fourteen lactic acid bacterial isolates were identified as Enterococcus mediterraneensis, Lactobacillus fermentum, and Streptococcus lutetiensis by 16S rRNA gene sequencing, and in vitro characterized for their actual probiotic potential. All E. mediterraneensis isolates were resistant to clindamycin, whereas Lb. fermentum isolates were resistant to ampicillin, clindamycin, and vancomycin. The E. mediterraneensis and Lb. fermentum isolates displayed high overall digestive survival, ranged from 1.35 ± 0.06 to 32.73 ± 0.84% and from 2.01 ± 0.01 to 23.9 ± 1.85%, respectively. All isolates displayed cell surface hydrophobicity, ranged between 15.44 ± 6.72 and 39.79 ± 2.87%. The strongest auto-aggregation capability, higher than 40%, was observed for most E. mediterraneensis and Lb. fermentum isolates. The E. mediterraneensis isolates (L2, L12, and L15), Lb. fermentum (L8, L9, and L10), and Strep. lutetiensis (L14) exhibited the greatest co-aggregation with Salmonella typhimurium, Escherichia coli O157:H7, Staphylococcus aureus, and Bacillus cereus. Fifty-seven and fourteen hundredth percent of E. mediterraneensis isolates could be considered bacteriocinogenic against E. coli O157:H7, B. cereus, and S. aureus. Conclusion This study is the first one to isolate Enterococcus mediterraneensis in Egypt and to characterize it as new species of probiotics globally. According to the results, E. mediterraneensis (L2, L12, and L15), Lb. fermentum (L8, L9, and L10), and Strep. lutetiensis (L14) are the most promising in vitro probiotic candidates.

scholarly journals A study of virulence factors of E. coli strains isolated from cases of urinary tract infection

2021 ◽  
Vol 8 (2) ◽  
pp. 163-167
Author(s):  
Sanjeev Dimri ◽  
Krati Varshney
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Cell Surface ◽  
Virulence Factors ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Clinical Microbiology Laboratory ◽  
E Coli ◽  
Tract Infections

The aim of this study was to demonstrate virulence factors namely adhesins, hemolysin and cell surface hydrophobicity of E. coli strains isolated from cases of urinary tract infection.A total of 210 E. coli strains were isolated from 602 culture positive cases of urinary tract infections. UPEC strains were screened for virulence factors namely hemolysin, hemagglutination and cell surface hydrophobicity by recommended methods. Out of 210 E. coli strains tested, 70 (33.34%) were hemolytic, 94 (44.76%) showed hemagglutination and 46 (21.90%) were cell surface hydrophobicity positive. Our study shows that a large number of uropathogenic E. coli strains had one or more virulence factors. The methods of detection of above-mentioned virulence factors are reasonably easy and screening them in a clinical microbiology laboratory is a worthwhile exercise.

Relationship of Cell Surface Charge and Hydrophobicity to Strength of Attachment of Bacteria to Cantaloupe Rind†

2002 ◽  
Vol 65 (7) ◽  
pp. 1093-1099 ◽  
Author(s):  
DIKE O. UKUKU ◽  
WILLIAM F. FETT
Keyword(s):  
Cell Surface ◽  
Surface Charge ◽  
Cell Surface Hydrophobicity ◽  
Hydrophobic Interaction Chromatography ◽  
Surface Hydrophobicity ◽  
Bacterial Attachment ◽  
Wash Water ◽  
Bacterial Surface ◽  
E Coli ◽  
Salmonella Infections

The cantaloupe melon has been associated with outbreaks of Salmonella infections. It is suspected that bacterial surface charge and hydrophobicity may affect bacterial attachment and complicate bacterial detachment from cantaloupe surfaces. The surface charge and hydrophobicity of strains of Salmonella, Escherichia coli (O157:H7 and non-O157:H7), and Listeria monocytogenes were determined by electrostatic and hydrophobic interaction chromatography, respectively. Initial bacterial attachment to cantaloupe surfaces and the ability of bacteria to resist removal by washing with water were compared with surface charge and hydrophobicity. Whole cantaloupes were submerged in inocula containing individual strains or in cocktails containing Salmonella, E. coli, and L. monocytogenes, either as a mixture of strains containing all three genera or as a mixture of strains belonging to a single genus, for 10 min. Inoculated cantaloupes were dried for 1 h in a biosafety cabinet and then stored for up to 7 days at 4°C. Inoculated melons were washed with water, and bacteria still attached to the melon surface, as well as those in the wash water, were enumerated. Initial bacterial attachment was highest for individual strains of E. coli and lowest for L. monocytogenes, but Salmonella exhibited the strongest attachment on days 0, 3, and 7. When mixed-genus cocktails were used, the relative degrees of attachment of the three genera ware altered. The attachment of Salmonella strains was the strongest, but the attachment of E. coli was more extensive than that of L. monocytogenes on days 0, 3, and 7. There was a linear correlation between bacterial cell surface hydrophobicity (r2 = 0.767), negative charge (r2 = 0.738), and positive charge (r2 = 0.724) and the strength of bacterial attachment to cantaloupe surfaces.

scholarly journals Study of uropathogenic Escherichia coli with special reference to its virulence factors

2017 ◽  
Vol 5 (1) ◽  
pp. 177 ◽  
Author(s):  
Sanjay Singh Kaira ◽  
Chitra Pai
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Virulence Factors ◽  
Cell Surface Hydrophobicity ◽  
Empirical Therapy ◽  
Surface Hydrophobicity ◽  
E Coli ◽  
Set Up ◽  
One Year ◽  
A Prospective Study

Background: Urinary tract infection (UTI) is one of the most common nosocomial infections, caused by Escherichia coli. UPEC differ from non-pathogenic E. coli by the production of specific virulence factors which enable the bacteria to adhere to uroepithelial cells and to establish UTI. The aim of this study is to check the virulence factors of uropathogenic E. coli.Methods: A prospective study conducted in the Department of Microbiology of a tertiary level hospital in Mumbai over a period of one year (February 2011 to February 2012). A total of 123 Urine samples received in the laboratory were processed as per standard microbiological procedures to look for virulence factors like hemolysin, hemagglutination, cell surface hydrophobicity and gelatinase production. Results: Out of 123 patients 69 (56.09%) cases were from females and 54 (43.90%) were males. Thus female: male ratio was 1:3. Hemolysin production was seen in 27.64%, hemagglutination in 53%, cell surface hydrophobicity in 27.64%. Conclusions: UTI is more common in middle aged females and in community set-up. The knowledge of virulence factors of E. coli will help in better understanding of the organism pathogenicity and guided empirical therapy can result in better treatment outcome. 

scholarly journals Evaluation of Multifarious Plant Growth Promoting Trials of Yeast Isolated from the Soil of Assam Tea (Camellia sinensis var. assamica) Plantations in Northern Thailand

2020 ◽  
Vol 8 (8) ◽  
pp. 1168
Author(s):  
Jaturong Kumla ◽  
Supakorn Nundaeng ◽  
Nakarin Suwannarach ◽  
Saisamorn Lumyong
Keyword(s):  
Plant Growth ◽  
Camellia Sinensis ◽  
Mycorrhizal Fungi ◽  
Yeast Species ◽  
Growth Promotion ◽  
Northern Thailand ◽  
Yeast Strains ◽  
Plant Growth Promoting ◽  
Assam Tea ◽  
Growth Promoting

Some soil microorganisms, especially bacteria and mycorrhizal fungi, play a role in the promotion of plant growth. However, plant growth promotion involving yeasts in soil has not yet been extensively investigated. This study aimed to isolate and identify yeast strains obtained from soils of the Assam tea plant (Camellia sinensis var. assamica) in northern Thailand and to investigate their plant growth promoting capabilities. A total of 42 yeast strains were obtained and identified by analysis of the D1/D2 domain of the large subunit ribosomal RNA gene. We identified 35 strains of six species belonging to the phylum Ascomycota, namely Aureobasidium melanogenum, Kazachstania aquatica, Saturnispora diversa, Saturnispora sekii, Schwanniomyces pseudopolymorphus and Wickerhamomyces anomalus, and six species were determined to belong to the phylum Basidiomycota, namely Apiotrichum scarabaeorum, Curvibasidium pallidicorallinum, Papiliotrema laurentii, Rhodosporidiobolus ruineniae, Trichosporon asahii and Trichosporon coremiiforme. Seven strains were representative of potential new species and belonged to the genera Galactomyces and Wickerhamomyces. A total of 28 strains were found to produce indole-3-acetic acid (IAA) in a range of 2.12 to 37.32 mg/L, with the highest amount of IAA produced by R. ruineniae SDBR-CMU-S1-03. All yeast strains were positive in terms of ammonia production, and only eight strains were positive for siderophore production. Two yeast species, P. laurentii and W. anomalus, were able to solubilize the insoluble form of calcium and zinc. The ability to produce amylase, endogulcanase, lipase, pectinase, protease and xylanase was dependent upon the yeast species and strain involved.

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