scholarly journals Genic microsatellite marker characterization and development in little millet (Panicum sumatrense) using transcriptome sequencing

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hiral Desai ◽  
Rasmieh Hamid ◽  
Zahra Ghorbanzadeh ◽  
Nishant Bhut ◽  
Shital M. Padhiyar ◽  
...  
Keyword(s):  
Gene Expression Regulation ◽  
Evolutionary Genetics ◽  
Future Research ◽  
Genic Ssr ◽  
Little Millet ◽  
Nutrient Value ◽  
Three Samples ◽  
Genetic Resource Conservation ◽  
Modern Genomic ◽  
Metabolism Gene

AbstractLittle millet is a climate-resilient and high-nutrient value plant. The lack of molecular markers severely limits the adoption of modern genomic approaches in millet breeding studies. Here the transcriptome of three samples were sequenced. A total of 4443 genic-SSR motifs were identified in 30,220 unigene sequences. SSRs were found at a rate of 12.25 percent, with an average of one SSR locus per 10 kb. Among different repeat motifs, tri-nucleotide repeat (66.67) was the most abundant one, followed by di- (27.39P), and tetra- (3.83P) repeats. CDS contained fewer motifs with the majority of tri-nucleotides, while 3′ and 5′ UTR carry more motifs but have shorter repeats. Functional annotation of unigenes containing microsatellites, revealed that most of them were linked to metabolism, gene expression regulation, and response to environmental stresses. Fifty primers were randomly chosen and validated in five little millet and 20 minor millet genotypes; 48% showed polymorphism, with a high transferability (70%) rate. Identified microsatellites can be a noteworthy resource for future research into QTL-based breeding, genetic resource conservation, MAS selection, and evolutionary genetics.

Clarifying the Relations between Intellectual Humility and Misinformation Susceptibility

2021 ◽  
Author(s):  
Shauna Marie Bowes ◽  
Arber Tasimi
Keyword(s):  
Political Ideology ◽  
Psychological Characteristics ◽  
Future Research ◽  
Fake News ◽  
Intellectual Humility ◽  
Conspiracy Theories ◽  
Cognitive Reflection ◽  
Three Samples

Misinformation is widespread and consequential. Thus, identifying psychological characteristics that might mitigate misinformation susceptibility represents a timely and pragmatically important issue. One construct that may be particularly relevant to misinformation susceptibility is intellectual humility (IH). As such, we examined whether IH is related to less misinformation susceptibility, what aspects of IH best predict misinformation susceptibility, and whether these relations are unique to IH. Across three samples, IH tended to manifest small-to-medium negative relations with misinformation susceptibility (pseudoscience, conspiracy theories, and fake news). IH measures assessing both intrapersonal and interpersonal features tended to be stronger correlates of misinformation susceptibility than measures assessing either intrapersonal or interpersonal features in isolation. These relations tended to remain robust after controlling for covariates (honesty-humility, cognitive reflection, political ideology). Future research should leverage our results to examine whether IH interventions not only reduce misinformation susceptibility but also lessen its appeal for those already committed to misinformation.

Development and Validation of the Short Version of the Sense of Humor Scale (SHS-S)

2021 ◽  
Author(s):  
Sonja Heintz ◽  
Willibald Ruch ◽  
Chloe Lau ◽  
Donald H. Saklofske ◽  
Paul McGhee
Keyword(s):  
Internal Consistency ◽  
Training Programs ◽  
Well Being ◽  
English Speakers ◽  
Future Research ◽  
Short Version ◽  
Sense Of Humor ◽  
Three Samples ◽  
English Speaking ◽  
Development And Validation

Abstract. Humor training has become increasingly popular to enhance the “sense of humor” and well-being and to decrease depressive symptoms. Despite the wide applications of these training programs, the assessment of training efficacy has attracted less attention. The Sense of Humor Scale (SHS; McGhee, 1996 , 1999 ) recently was expanded to a long version (SHS-L) to enhance its internal consistency ( Ruch & Heintz, 2018 ). At the same time, there is also the need for a brief version of this scale. The purpose of the present study is to develop a short version (SHS-S) in both German- and English-speaking countries, test its psychometric properties (internal consistency, factorial, construct, and criterion validity), and assess measurement invariance across gender and the two languages. Using three samples (Sample 1: 570 English-speakers, Sample 2: 353 German-speakers, Sample 3: 94 other-reports), the 29-item SHS-S was developed and yielded promising internal consistency and validity scores for the six humor skill factors of enjoyment of humor, laughter, verbal humor, finding humor in everyday life, laughing at yourself, and humor under stress. Overall, the SHS-S is an internally consistent, valid, and economic tool for future research and group-based applications, while the SHS-L seems especially useful in individual applications.

scholarly journals Statistical Studies of the Clustering of Galaxies

1980 ◽  
Vol 92 ◽  
pp. 31-38
Author(s):  
Edward J. Groth
Keyword(s):  
Correlation Function ◽  
Correlation Functions ◽  
Future Research ◽  
Three Samples ◽  
Statistical Studies

An investigation of the clustering of galaxies making use of correlation function techniques is discussed. Correlation functions are defined and the relations between the angular and spatial functions are presented. The results obtained from application of the method to three samples of galaxies are described and some conclusions are drawn. Finally, some directions for future research are suggested.

scholarly journals The Evolutionary Persistence of Genes That Increase Mental Disorders Risk

2008 ◽  
Vol 17 (6) ◽  
pp. 395-399 ◽  
Author(s):  
Matthew C. Keller
Keyword(s):  
Natural Selection ◽  
Mental Disorders ◽  
Evolutionary Genetics ◽  
Future Research ◽  
Human Populations ◽  
Psychiatric Genetics ◽  
Severe Mental Disorders ◽  
Risk Alleles ◽  
Deleterious Alleles ◽  
Evolutionary Persistence

Natural selection constantly removes those genetic variants (alleles) that even slightly decrease average reproductive success. Yet, given the high heritabilities and prevalence rates of severe mental disorders, human populations seem to be awash in deleterious alleles. Evolutionary genetics offers an illuminating framework for understanding why mental disorder risk alleles have persisted despite natural selection, and this framework can help guide future research in behavioral and psychiatric genetics.

scholarly journals Activity and Function Studies of The Promoter Cis-Acting Elements of The Key Enzymes in Saponins Biosynthesis of DS From Panax Notoginseng

2020 ◽  
Author(s):  
Yujie Zheng ◽  
Rongyu Shao ◽  
Pengguo Xia ◽  
Zongsuo Liang ◽  
Kaijing Yan
Keyword(s):  
Gene Expression ◽  
Gene Expression Regulation ◽  
Panax Notoginseng ◽  
Promoter Sequence ◽  
Upstream Region ◽  
Future Research ◽  
Active Components ◽  
Cis Acting ◽  
Plant Expression Vector ◽  
Important Region

Abstract Background：Panax notoginseng is a traditional Chinese medicine for the treatment of blood diseases, in which saponins were the main active components. Dammarenediol synthase (DS) is a key enzyme in the saponin synthesis pathway of P. notoginseng.The promoter is an important region to regulate gene expression, and the study of the promoter sequence provides important evidence for revealing the mechanism of gene expression regulation. Methods: However, there was still little research on the promoter function of P. notoginseng. In this study, the 1382 bp promoter upstream of DS from P. notoginseng was cloned and sequenced. The promoter sequence was analyzed by online databases. The plant expression vector fused with the β-glucuronidase gene was constructed, transferred into Agrobacterium tumefaciens. Then tobacco was injected, and its response to exogenous hormones (gibberellin and abscisic acid) was studied by transient expression to verify its unique action elements. Results: The results showed that the tobacco leaves transferred with DS promoter had significantly increased GUS protease activity after spraying GA and ABA, indicating that both DS promoter can specifically and significantly respond to exogenous GA and ABA signal. Conclusions: These findings will help us to better understand the regulatory mechanisms of the upstream region of the DS gene and provide a basis for future research on the interaction of cis-acting elements of promoters with related transcription factors.

scholarly journals Fluctuations in grandiose and vulnerable narcissistic states: A momentary perspective

2019 ◽  
Author(s):  
Elizabeth A. Edershile ◽  
Aidan G.C. Wright
Keyword(s):  
Community Sample ◽  
Self Esteem ◽  
Borderline Personality ◽  
Future Research ◽  
Psychological Processes ◽  
Three Samples ◽  
Exploratory Approach ◽  
Lagged Effects ◽  
Low Levels ◽  
Total Observation

Theories of narcissism emphasize the dynamic processes within and between grandiosity and vulnerability. Research seeking to address this has either not studied grandiosity and vulnerability together or has used dispositional measures to assess what are considered to be momentary states. Emerging models of narcissism suggest grandiosity and vulnerability can further be differentiated into a three-factor structure – Exhibitionistic Grandiosity, Entitlement, and Vulnerability. Research in other areas of maladaptive personality (e.g., borderline personality disorder) has made headway in engaging data collection and analytic methods that are specifically meant to examine such questions. The present study took an exploratory approach to studying fluctuations within and between grandiose and vulnerable states. Fluctuations – operationalized as gross variability, instability, and lagged effects – were examined across three samples (two undergraduate and a community sample oversampled for narcissistic features; Total person N = 862; Total observation N = 36,631). Results suggest variability in narcissistic states from moment to moment is moderately associated with dispositional assessments of narcissism. Specifically, individuals who are dispositionally grandiose express both grandiosity and vulnerability, and vary in their overall levels of grandiosity and vulnerability over time. On the other hand, dispositionally vulnerable individuals tend to have high levels of vulnerability and low levels of grandiosity. Entitlement plays a key role in the processes that underlie narcissism and narcissistic processes appear unique to the construct and not reflective of broader psychological processes (e.g., self-esteem). Future research should consider using similar methods and statistical techniques on different timescales to study dynamics within narcissism.

scholarly journals The Self-Generated Stress Scale: Development, Psychometric Features, and Associations With Perfectionism, Self-Criticism, and Distress

2019 ◽  
Vol 38 (1) ◽  
pp. 69-83
Author(s):  
Gordon L. Flett ◽  
Paul L. Hewitt ◽  
Taryn Nepon
Keyword(s):  
The Self ◽  
Self Report ◽  
Future Research ◽  
Self Presentation ◽  
Stress Scale ◽  
Three Samples ◽  
Current Article ◽  
Challenging Situation ◽  
Development And Validation ◽  
Report Measure

In the current article, we describe the development and validation of a self-report measure of self-generated stress and its associations with measures of perfectionism, self-criticism, and distress. The Self-Generated Stress Scale is a seven-item inventory that taps the tendency to see oneself as someone who generates and adds to existing personal stress (i.e., making a challenging situation worse or turning a life problem into a bigger problem). Psychometric analyses with data from three samples of university students showed that the Self-Generated Stress Scale has one factor and acceptable internal consistency. Analyses established that self-generated stress is associated with trait perfectionism, self-criticism, dependency, and self-silencing, as well as indices tapping cognitive perfectionism and perfectionistic self-presentation. Self-generated stress was also associated with distress and psychosomatic health symptoms. Regression analyses established that scores on the Self-Generated Stress Scale predict unique variance in distress and negative affect beyond the variance attributable to self-criticism and other measures of stress. Overall, our findings attest to the further use of the Self-Generated Stress Scale in various contexts and highlight that certain perfectionists suffer from a perceived tendency to make their lives more stressful. The implications of these findings are discussed along with directions for future research.

scholarly journals Synthetic Biology-Based Approaches for Microalgal Bio-Removal of Heavy Metals From Wastewater Effluents

2021 ◽  
Vol 9 ◽  
Author(s):  
Pachara Sattayawat ◽  
Ian S. Yunus ◽  
Nuttapol Noirungsee ◽  
Nilita Mukjang ◽  
Wasu Pathom-Aree ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Synthetic Biology ◽  
Gene Expression Regulation ◽  
Future Research ◽  
Design Build ◽  
Removal Of Heavy Metals ◽  
Cellular Tolerance ◽  
Improved Characteristics ◽  
Wastewater Effluents

Heavy metal polluted wastewater from industries is currently one of the major environmental concerns leading to insufficient supply of clean water. Several strategies have been implemented to overcome this challenge including the use of microalgae as heavy metal bio-removers. However, there are still limitations that prevent microalgae to function optimally. Synthetic biology is a new biological discipline developed to solve challenging problems via bioengineering approaches. To date, synthetic biology has no universally affirmed definitions; however, it is uncontroversial that synthetic biology utilizes a constructive library of genetic standardized parts to create new biological systems or to redesign existing ones with improved characteristics. In this mini-review, we present state-of-the-art synthetic biology-based approaches that can be used to enhance heavy metal bio-removal from wastewater effluents by microalgae with a narrative synthetic biology workflow (Design-Build-Test-Learn cycle) to guide future developments of more advanced systems. We also provide insights into potent genes and proteins responsible for the bio-removal processes for stepwise developments of more advanced systems. A total of 49 unique genes and proteins are listed based on their eight heavy metals (Mn, Fe, Cu, Zn, As, Cd, Hg, and Pb) bio-removal functions in transport system, cellular tolerance, synthesis of key players in heavy metal bio-removal, biotransformation of heavy metals, and gene expression regulation. Thus, with our library, genetic parts are ready to be recruited for any synthetic biology-based designs. Thereby, this mini-review identifies potential avenues of future research and maps opportunities to unleash more potential of microalgae as heavy metal bio-removers with synthetic biology.

Beyond the Bottom Line: Don’t Forget to Consider the Role of the Family

2021 ◽  
pp. 014920632110305
Author(s):  
Matthew J. Quade ◽  
Maggie Wan ◽  
Dawn S. Carlson ◽  
K. Michele Kacmar ◽  
Rebecca L. Greenbaum
Keyword(s):  
Organizational Commitment ◽  
Spillover Effect ◽  
Future Research ◽  
Work Family Conflict ◽  
Bottom Line ◽  
Family Work ◽  
Work Conflict ◽  
Three Samples ◽  
Work Family

Our work investigates the influence of supervisor bottom-line mentality (SBLM) (i.e., a one-dimensional focus on bottom-line outcomes to the exclusion of other organizational priorities) on employees’ organizational commitment via the work-family interface as well as the crossover effects of SBLM on the organizational commitment of the employees’ spouse. More specifically, we examined how SBLM contributes to work-family conflict (WFC) and impacts the experienced commitment of the dyad along three paths. We conducted two studies across three samples (Study 1, Sample A: 186 employees; Study 1, Sample B: 258 employees; Study 2: 399 employee-spouse dyads) to demonstrate the unique role of SBLM in this context and find support for the hypothesized relationships. First, the resource drain of SBLM had a spillover effect through WFC to decrease the employee’s commitment at work. Second, it crossed over to the spouse to reduce their own organizational commitment due to the employee being a source of family undermining, which subsequently influenced the spouse’s family-work conflict (FWC). Third, SBLM impacted the spouse such that it crossed back to contribute to decreased organizational commitment for the employee. Theoretical contributions and practical implications are discussed as well as directions for future research.

Politics as a Moderator of the Accountability—Job Satisfaction Relationship: Evidence Across Three Studies

2008 ◽  
Vol 35 (2) ◽  
pp. 307-326 ◽  
Author(s):  
Denise M. Breaux ◽  
Timothy P. Munyon ◽  
Wayne A. Hochwarter ◽  
Gerald R. Ferris
Keyword(s):  
Job Satisfaction ◽  
Theory And Practice ◽  
Future Research ◽  
Work Settings ◽  
Three Samples ◽  
Main Effect ◽  
Interactive Relationship ◽  
Felt Accountability ◽  
Organizational Tenure ◽  
Satisfaction Relationship

The current study examined the interactive relationship between felt accountability and organizational politics perceptions on job satisfaction in three samples, which included employees in a wide array of work settings. We hypothesized that job satisfaction would decline (increase) as felt accountability became more intense for those perceiving heightened (limited) levels of politics. After controlling for demographic factors (e.g., age, gender, and organizational tenure), affectivity (e.g., negative and positive), and nonlinear main effect terms (e.g., felt accountability2 and politics perceptions2), results supported the study's hypothesized relationship. Namely, findings indicated that accountability predicted unfavorable levels of job satisfaction when coupled with heightened politics perceptions. Implications of results for theory and practice, strengths and limitations, and directions for future research are provided.

Sign in / Sign up

Export Citation Format

Share Document