scholarly journals A novel subgenotype C6 Enterovirus A71 originating from the recombination between subgenotypes C4 and C2 strains in mainland China

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Yongjuan Liu ◽  
Jingyi Zhou ◽  
Guangquan Ji ◽  
Yupeng Gao ◽  
Chunyan Zhang ◽  
...  
Keyword(s):  
Mainland China ◽  
Disease Outbreaks ◽  
Unknown Origin ◽  
Genetic Distances ◽  
Genetic Characteristics ◽  
Enterovirus A71 ◽  
Distinct Cluster ◽  
New Variant ◽  
Genotype C ◽  
Reference Strains

AbstractRecombination plays important roles in the genetic diversity and evolution of Enterovirus A71 (EV-A71). The phylogenetics of EV-A71 in mainland China found that one strain DL71 formed a new subgenotype C6 with unknown origin. This study investigated the detailed genetic characteristics of the new variant. DL71 formed a distinct cluster within genotype C based on the genome and individual genes (5′UTR, VP4, VP1, 2A, 2B, 2C, 3D, and 3′UTR). The average genetic distances of the genome and individual genes (VP3, 2A, 2B, 2C, 3A, 3C, and 3D) between DL71 and reference strains were greater than 0.1. Nine recombination events involving smaller fragments along DL71 genome were detected. The strains Fuyang-0805a (C4) and Tainan/5746/98 (C2) were identified as the parental strains of DL71. In the non-recombination regions, DL71 had higher identities with Fuyang-0805a than Tainan/5746/98, and located in the cluster with C4 strains. However, in the recombination regions, DL71 had higher identities with Tainan/5746/98 than Fuyang-0805a, and located in the cluster with C2 strains. Thus, DL71 was a novel multiple inter-subgenotype recombinant derived from the dominant subgenotype C4 and the sporadic subgenotype C2 strains. Monitoring the emergence of new variants by the whole-genome sequencing remains essential for preventing disease outbreaks and developing new vaccines.

scholarly journals Molecular epidemiological characteristics of echovirus 6 in mainland China: extensive circulation of genotype F from 2007 to 2018

2021 ◽  
Author(s):  
Wenjun Cheng ◽  
Tianjiao Ji ◽  
Shuaifeng Zhou ◽  
Yong Shi ◽  
Lili Jiang ◽  
...  
Keyword(s):  
Common Ancestor ◽  
Mainland China ◽  
Recent Common Ancestor ◽  
Genetic Characteristics ◽  
Most Recent Common Ancestor ◽  
Significant Difference ◽  
Echovirus 6 ◽  
Epidemiological Characteristics ◽  
Highest Posterior Density ◽  
Genotype F

AbstractEchovirus 6 (E6) is associated with various clinical diseases and is frequently detected in environmental sewage. Despite its high prevalence in humans and the environment, little is known about its molecular phylogeography in mainland China. In this study, 114 of 21,539 (0.53%) clinical specimens from hand, foot, and mouth disease (HFMD) cases collected between 2007 and 2018 were positive for E6. The complete VP1 sequences of 87 representative E6 strains, including 24 strains from this study, were used to investigate the evolutionary genetic characteristics and geographical spread of E6 strains. Phylogenetic analysis based on VP1 nucleotide sequence divergence showed that, globally, E6 strains can be grouped into six genotypes, designated A to F. Chinese E6 strains collected between 1988 and 2018 were found to belong to genotypes C, E, and F, with genotype F being predominant from 2007 to 2018. There was no significant difference in the geographical distribution of each genotype. The evolutionary rate of E6 was estimated to be 3.631 × 10-3 substitutions site-1 year-1 (95% highest posterior density [HPD]: 3.2406 × 10-3-4.031 × 10-3 substitutions site-1 year-1) by Bayesian MCMC analysis. The most recent common ancestor of the E6 genotypes was traced back to 1863, whereas their common ancestor in China was traced back to around 1962. A small genetic shift was detected in the Chinese E6 population size in 2009 according to Bayesian skyline analysis, which indicated that there might have been an epidemic around that year.

scholarly journals Molecular epidemiology and recombination of Enterovirus A71 in mainland China from 1987 to 2017

2021 ◽  
Author(s):  
Jingyi Zhou ◽  
Yingying Shi ◽  
Li Miao ◽  
Chunyan Zhang ◽  
Yongjuan Liu
Keyword(s):  
Molecular Epidemiology ◽  
Mainland China ◽  
Enterovirus A71

Enterovirus Diseases and Vaccines

2021 ◽  
Author(s):  
Heinz-Josef Schmitt
Keyword(s):  
Disease Outbreaks ◽  
Foot And Mouth Disease ◽  
Asia Pacific ◽  
Asia Pacific Region ◽  
Internal Organs ◽  
Enterovirus A71 ◽  
Life Threatening ◽  
Development Pipeline ◽  
The Brain ◽  
Genus Enterovirus

Enterovirus A71 (EV A71) (genus enterovirus, family pircornaviridae) causes benign vesicular lesions on skin (hand, foot and mouth disease, HFMD) and mucous membranes of the mouth (herpangina), and also severe to life-threatening infections of the brain, the heart, and other internal organs. Disease outbreaks in the Asia-Pacific region regularly involve thousands of children <5 years resulting in many deaths. Such outbreaks are caused by specific EV genotypes that vary by time and place. While there are various promising and innovative options for treatment in development, none are licensed to date. Immunoglobulins may be beneficial through virus neutralization and modulation of the inflammatory response by the host. In China, 3 different highly efficacious and safe vaccines are commercially available; however, none are licensed outside the country. Roughly half a dozen vaccines are in the development pipeline, with some using innovative approaches and trying to broaden strain coverage.

scholarly journals Focus on the spondyloarthritides. Can earlier diagnosis change the course of the disease?

2013 ◽  
pp. 277-283
Author(s):  
Domenico Galasso ◽  
Giovanni Forte ◽  
Norma Marigliano
Keyword(s):  
Strong Association ◽  
Human Leukocyte ◽  
Unknown Origin ◽  
Lower Limbs ◽  
Inflammatory Rheumatic Diseases ◽  
Genetic Characteristics ◽  
Leukocyte Antigen ◽  
Musculoskeletal Manifestations ◽  
Chronic Inflammatory Rheumatic Diseases ◽  
Juvenile Spondyloarthritis

The spondyloarthritides (or spondyloarthropathies) (SPAs) are chronic, inflammatory, rheumatic diseases of unknown origin, which share certain clinical, epidemiological, and genetic characteristics. They include ankylosing spondylitis, reactive arthritis (also known as the Reiter Syndrome), psoriatic arthritis, enteropathic spondyloarthropathy (ulcerative colitis, Crohn’s disease), undifferentiated spondyloarthritis, juvenile spondyloarthritis, and formes frustes such as acute anterior uveitis, spondyloarthritic carditis, and balanitis circinata. In the past, the SPAs were considered variants of rheumatoid arthritis, but it is now clear that they differ from the latter disease in terms of the pattern of articular and extra-articular involvement, their lack of association with seropositivity for rheumatoid factor, and their strong association with sacro-iliac joint bacino= pelvis sacro-iliac joint sacro-iliac joint sacro-iliac joint sacro-iliac joint sacro-iliac joint the class I human leukocyte antigen B27. sacro-iliac joint bacino= pelvis sacro-iliac joint sacro-iliac joint sacro-iliac joint sacro-iliac joint sacro-iliac joint Their general characteristics are axial involvement; enthesitis; peripheral arthritis involving the lower limbs, which is usually asymmetric; dactylitis; extra-articular manifestations involving the skin, eyes, bowel, and genitals. The musculoskeletal manifestations of the SPAs are due to inflammation at the level of the entheses. It is important to distinguish between the numerous clinical SPA variants based on analysis of symptoms, laboratory tests, and instrumental studies. Thanks to a greater understanding of the pathogenesis of the SPAs and the widespread availability of highly sensitive imaging modalities for their diagnosis, it is now possible to identify these diseases early and modify their course with effective therapy. This approach offers benefits to patients in terms of reduced morbidity and mortality and improved quality of life.

scholarly journals 42 Genetic differentiation of Russian goats and wild relatives based on microsatellite loci

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 19-20
Author(s):  
Marina I Selionova ◽  
Magomet M Aibazov ◽  
Tatyana V Mamontova ◽  
Sergey N Petrov ◽  
Veronica R Kharzinova ◽  
...  
Keyword(s):  
Genetic Distances ◽  
Wild Relatives ◽  
Hybrid Zones ◽  
Sample Collection ◽  
North Caucasus ◽  
Genetic Characteristics ◽  
The Third ◽  
The North ◽  
Central Caucasus ◽  
Capra Aegagrus

Abstract Goat breeding is a traditional livestock industry for many regions of Russia. Due to unpretentiousness and high adaptive qualities, the Karachai, Dagestan wool and Dagestan down breeds are widespread in the North Caucasus. Moreover, their uniqueness is determined by sharing a common habitat with their wild relatives, West Caucasian and Dagestani turs, thus forming hybrid zones. The aim of the research was to study allele pool and interbreed differentiation of domestic goat breeds and wild relatives by microsatellites. Research was performed based on 16 loci on the ABI 3130xl analyzer. Population genetic characteristics were calculated in GenAlEx 6.5, and the phylogenetic tree was constructed in Populations 1.2.30 and SplitsTree 4.14.5. The sample collection included goat breeds (n = 226): Karachai (KRCH), Dagestan down (DAGD), Dagestan wool (DAGW) (North Caucasus), Soviet wool (SOVW) (Siberia), Orenburg (OREN) (South Ural), and wild goats (n = 72). Wild goats comprised mountain goats: Siberian goats (Capra sibirica) from the Altai, Tajikistan, Kyrgyzstan and the Himalayas; bezoar goats (Capra aegagrus) from Turkey and Pakistan; and tur (Capra caucasica) from the West Caucasus (Kuban), Central Caucasus, and East Caucasus (Dagestan). The Karachai breed was characterized by the highest level of genetic diversity: the average number of alleles per locus (NA) and allelic richness (AR) were 9,154 and 7,713, respectively, while these indices varied from 6,538 to 7,538 and from 6,425 to 7,538 (Table 1) in other breeds, respectively. The values of genetic distances showed differentiation of Karachai, Dagestan down and Dagestan wool breeds from Orenburg and Soviet wool breeds (Table 2). The formation of three clusters was established. The first one included subspecies of the Caucasian tur, the second one comprised Siberian goats, and the third contained domestic goats. Bezoar goats were in the root of the third cluster that confirmed their participation as an ancestral form of domestic goats.

scholarly journals A Review of Psychological Issues among Patients and Healthcare Staff during Two Major Coronavirus Disease Outbreaks in China: Contributory Factors and Management Strategies

2020 ◽  
Vol 17 (18) ◽  
pp. 6673 ◽  
Author(s):  
Ka Ming Chow ◽  
Bernard M.H. Law ◽  
Marques S.N. Ng ◽  
Dorothy N.S. Chan ◽  
Winnie K.W. So ◽  
...  
Keyword(s):  
Psychological Symptoms ◽  
Management Strategies ◽  
Mainland China ◽  
Disease Outbreaks ◽  
Well Being ◽  
Healthcare Services ◽  
Healthcare Staff ◽  
Quality Healthcare ◽  
Psychological Issues ◽  
Counselling Services

Outbreaks of severe acute respiratory syndrome (SARS) and coronavirus disease 2019 (COVID-19) have affected populations worldwide. Our literature review summarises the studies reporting psychological issues among healthcare staff and infected patients in mainland China, Hong Kong, and Taiwan during these two outbreaks and the potential strategies for addressing these issues. Our review shows that patients and healthcare staff presented similar psychological symptoms, including anxiety, fear, distress, and depression, which may lead to stress-related complications such as insomnia. In patients, these psychological impairments can be contributed to by being quarantined, perceptions of threats to life, and uncertainty about health status. Quarantine is also a factor for distress among healthcare staff, together with their heavy workload, the fear that they and their families would become infected, witnessing their patients’ poor and deteriorating conditions, and the requirement to wear protective gear. Strategies that are needed to address these factors include providing counselling services, implementing mindfulness-based therapies and optimism interventions, and providing telecommunication facilities for patients to communicate with their families. Healthcare staff should also be provided with these services, together with appropriate and flexible work shift arrangements and morale boosting. These strategies would improve not only the mental well-being of patients and healthcare staff, but also the self-efficacy and competence of the staff to provide quality healthcare services.

Geographic dimorphism in the mountain brushtail possum (Trichosurus caninus): the case for a new species

2002 ◽  
Vol 50 (4) ◽  
pp. 369 ◽  
Author(s):  
D. B. Lindenmayer ◽  
J. Dubach ◽  
K. L. Viggers
Keyword(s):  
New Species ◽  
New South ◽  
New South Wales ◽  
Distinct Species ◽  
Genetic Distances ◽  
Brushtail Possum ◽  
Genetic Characteristics ◽  
Simple Index ◽  
South Wales ◽  
Eastern Australia

The morphological and genetic characteristics of the mountain brushtail possum (Trichosurus caninus) are described for animals from a range of locations throughout its known geographic distribution in eastern Australia. Although there is considerable variation among populations, unequivocal morphological and genetic differences exist between northern and southern populations of the species. Specimens from southern populations (from Victoria) have a significantly (P < 0.001) larger ear conch, a significantly (P < 0.001) longer pes, and a significantly (P < 0.001) shorter tail than do specimens from northern populations (from New South Wales and Queensland). Animals can be clearly distinguished using a simple index based on these three morphological measures, which are gathered from live animals. North–south dimorphism is strongly supported by patterns in genetic data that show genetic distances of 2.7–3.0% between the southern and northern populations. The combined outcomes of morphological and genetic analyses suggest the existence of two distinct species. We recommend that the northern form, distributed from central New South Wales north to central Queensland, retain the name Trichosurus caninus; the southern form from Victoria is described here as Trichosurus cunninghami, sp. nov. The common names of these new species should be the 'short-eared possum' and the 'mountain brushtail possum', respectively.

Heterogeneity among Isolates of Vibrio vulnificus Recovered from Eels (Anguilla anguilla) in Denmark

1998 ◽  
Vol 64 (12) ◽  
pp. 4676-4682 ◽  
Author(s):  
Lise Høi ◽  
Inger Dalsgaard ◽  
Angelo DePaola ◽  
Ronald J. Siebeling ◽  
Anders Dalsgaard
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Vibrio Vulnificus ◽  
Disease Outbreaks ◽  
Anguilla Anguilla ◽  
Phage Typing ◽  
Capsule Type ◽  
Intestinal Contents ◽  
Plasmid Profiling ◽  
Reference Strains

ABSTRACT The findings of this study demonstrate that Vibrio vulnificus isolates recovered from diseased eels in Denmark are heterogeneous as shown by O serovars, capsule types, ribotyping, phage typing, and plasmid profiling. The study includes 85 V. vulnificus isolates isolated from the gills, intestinal contents, mucus, spleen, and kidneys of eels during five disease outbreaks on two Danish eel farms from 1995 to 1997, along with a collection of 12V. vulnificus reference strains. The results showed that more than one serovar may be capable of causing disease in eels and that these isolates are genetically heterogenous as shown by ribotyping. Ribotyping also showed that the same isolates may persist in an eel farm and cause recurrent outbreaks. Phage typing did not correlate with ribotyping or serotyping. However, we observed that 26 of 28 isolates, which were not susceptible to any of the phages, showed the same ribotype, O serovar, and capsule type. This suggests that these isolates may possess features that make them resistant to lysis by the phages used in this study. Ninety-three of 97 isolates harbored between one and three high-molecular-weight plasmids which previously had been suggested to be associated with eel virulence. The subdivision of V. vulnificus into two biotypes based on the indole reaction can no longer be supported, since 82 of 97 isolates in this study were indole positive, and a subdivision into serovars appears to be more correct.

scholarly journals Identification, Phylogenetic Analysis, and Biological Characterization of Serratia marcescens Strains Causing Cucurbit Yellow Vine Disease

2003 ◽  
Vol 93 (10) ◽  
pp. 1233-1239 ◽  
Author(s):  
J. Rascoe ◽  
M. Berg ◽  
U. Melcher ◽  
F. L. Mitchell ◽  
B. D. Bruton ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Serratia Marcescens ◽  
16S Rdna ◽  
Acid Methyl Ester ◽  
Bacterial Strains ◽  
Metabolic Functions ◽  
Distinct Cluster ◽  
Cucurbit Yellow Vine Disease ◽  
Vine Decline ◽  
Reference Strains

A serious vine decline of cucurbits known as cucurbit yellow vine disease (CYVD) is caused by rod-shaped bacteria that colonize the phloem elements. Sequence analysis of a CYVD-specific polymerase chain reaction (PCR)-amplified 16S rDNA product showed the microbe to be a γ-proteobacterium related to the genus Serratia. To identify and characterize the bacteria, one strain each from watermelon and zucchini and several noncucurbit-derived reference strains were subjected to sequence analysis and biological function assays. Taxonomic and phylogenetic placement was investigated by analysis of the groE and 16S rDNA regions, which were amplified by PCR and directly sequenced. For comparison, eight other bacterial strains identified by others as Serratia spp. also were sequenced. These sequences clearly identified the CYVD strains as Serratia marcescens. However, evaluation of metabolic and biochemical features revealed that cucurbit-derived strains of S. marcescens differ substantially from strains of the same species isolated from other environmental niches. Cucurbit strains formed a distinct cluster, separate from other strains, when their fatty acid methyl ester profiles were analyzed. In substrate utilization assays (BIOLOG, Vitek, and API 20E), the CYVD strains lacked a number of metabolic functions characteristic for S. marcescens, failing to catabolize 25 to 30 compounds that were utilized by S. marcescens reference strains. These biological differences may reflect gene loss or repression that occurred as the bacterium adapted to life as an intracellular parasite and plant pathogen.

Sign in / Sign up

Export Citation Format

Share Document