scholarly journals Comparison of oral metabolome profiles of stimulated saliva, unstimulated saliva, and mouth-rinsed water

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Yuki Maruyama ◽  
Yuichiro Nishimoto ◽  
Kouta Umezawa ◽  
Ryosuke Kawamata ◽  
Yuko Ichiba ◽  
...  
Keyword(s):  
Profile Analysis ◽  
Saliva Sample ◽  
Biological Information ◽  
Sample Collection ◽  
Metabolome Analysis ◽  
Flight Mass Spectrometry ◽  
Unstimulated Saliva ◽  
Efficient Alternative ◽  
Metabolome Profiling ◽  
The Relationship

AbstractSaliva includes a substantial amount of biological information, which has enabled us to understand the relationship between oral metabolites and various oral and systemic disorders. However, collecting saliva using a controlled protocol is time-consuming, making saliva an unsuitable analyte in large cohort studies. Mouth-rinsed water (MW), the water used to rinse the mouth, can be collected easily in less time with less difference between subjects than saliva and could be used as an alternative in oral metabolome analyses. In this study, we investigated the potential of MW collection as an efficient alternative to saliva sample collection for oral metabolome profiling. MW, stimulated saliva, and unstimulated saliva were collected from 10 systemically healthy participants. The samples were subjected to metabolome analysis using capillary electrophoresis time-of-flight mass spectrometry, and the types and amounts of metabolites in the samples were compared. Qualitatively, MW contained the same metabolites as unstimulated and stimulated saliva. While the quantity of the metabolites did not drastically change between the sampling methods, all three reflected individual differences, and the features of MW were the same as those of the unstimulated saliva. Overall, these results suggest that MW may be an appropriate alternative to saliva in oral metabolome profile analysis.

scholarly journals Global Metabolome Analysis of Dunaliella tertiolecta, Phaeobacter italicus R11 Co-cultures using Thermal Desoprtion - Comprehensive Two-dimensional Gas Chromatography - Time-of-Flight Mass Spectrometry

2021 ◽  
Author(s):  
Michael D S Armstrong ◽  
Oscar René Arredondo Campos ◽  
Catherine C Bannon ◽  
Paulina de la Mata ◽  
Rebecca J Case ◽  
...  
Keyword(s):  
Chemical Nature ◽  
Growth Conditions ◽  
Dunaliella Tertiolecta ◽  
Two Dimensional ◽  
Metabolomic Profile ◽  
Metabolome Analysis ◽  
Flight Mass Spectrometry ◽  
Microalgae Oil ◽  
The Relationship ◽  
Potential Carbon

Dunaliella tertiolecta is a marine microalgae that has been studied extensively as a potential carbon-neutral biofuel source. Microalgae oil contains high quantities of energy-rich fatty acids and lipids, but is not yet commercially viable as an alternative fuel. Carefully optimised growth conditions, and more recently, algal-bacterial co-cultures have been explored as a way of improving the yield of Dunaliella tertiolecta microalgae oils. The relationship between the host microalgae and bacterial co-cultures is currently poorly understood. Here, a complete workflow is proposed to analyse the global metabolomic profile of co-cultured Dunaliella tertiolectra and Phaeobacter italicus R11, which will enable researchers to explore the chemical nature of this relationship in more detail.

scholarly journals Plasma Metabolome Profiling Identifies Metabolic Subtypes of Pancreatic Ductal Adenocarcinoma

Cells ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1821
Author(s):  
Ujjwal Mukund Mahajan ◽  
Ahmed Alnatsha ◽  
Qi Li ◽  
Bettina Oehrle ◽  
Frank-Ulrich Weiss ◽  
...  
Keyword(s):  
Pancreatic Ductal Adenocarcinoma ◽  
Enrichment Analysis ◽  
Response Prediction ◽  
Ductal Adenocarcinoma ◽  
Pathway Enrichment Analysis ◽  
Metabolome Analysis ◽  
Dismal Prognosis ◽  
Chemotherapeutic Response ◽  
Plasma Metabolome ◽  
Metabolome Profiling

Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers. Developing biomarkers for early detection and chemotherapeutic response prediction is crucial to improve the dismal prognosis of PDAC patients. However, molecular cancer signatures based on transcriptome analysis do not reflect intratumoral heterogeneity. To explore a more accurate stratification of PDAC phenotypes in an easily accessible matrix, plasma metabolome analysis using MxP® Global Profiling and MxP® Lipidomics was performed in 361 PDAC patients. We identified three metabolic PDAC subtypes associated with distinct complex lipid patterns. Subtype 1 was associated with reduced ceramide levels and a strong enrichment of triacylglycerols. Subtype 2 demonstrated increased abundance of ceramides, sphingomyelin and other complex sphingolipids, whereas subtype 3 showed decreased levels of sphingolipid metabolites in plasma. Pathway enrichment analysis revealed that sphingolipid-related pathways differ most among subtypes. Weighted correlation network analysis (WGCNA) implied PDAC subtypes differed in their metabolic programs. Interestingly, a reduced expression among related pathway genes in tumor tissue was associated with the lowest survival rate. However, our metabolic PDAC subtypes did not show any correlation to the described molecular PDAC subtypes. Our findings pave the way for further studies investigating sphingolipids metabolisms in PDAC.

scholarly journals Genetic discontinuity analysis of Collared Dove (Streptopelia decaocto)

2017 ◽  
pp. 5-11
Author(s):  
Zoltán Bagi ◽  
Szilvia Kusza
Keyword(s):  
Principal Component ◽  
Dispersion Pattern ◽  
Genetic Isolation ◽  
Sample Collection ◽  
Mitochondrial Coi ◽  
Genetic Structure Of Populations ◽  
Genetic Discontinuity ◽  
Genetic Barriers ◽  
Coi Sequences ◽  
The Relationship

The Collared Dove conquered continent areas within a few decades. Causes and dispersion pattern of expansion has been investigated in several studies. However, the relationship between the geographic distribution and genetic structure of populations has not been researched. We used 152 individuals from 19 countries in this study. We analyze a 650 bp long mitochondrial COI sequences of each individuals. We were performed Spatial Autocorrelation Analysis, Principal Component Analysis and analysis of the genetic discontinuity in this study. Under 2500 km distance was a positive correlation between the genetic differentiation and different geographical areas. Hidden genetic barriers were found only Carpatian Basin. Could not be detected signs of genetic isolation in other regions. This will probably due to the unevenness of the sample collection, because these areas proportionally much fewer sequences were available. Therefore, is worth repeat this analysis after further sample collection, in the future.

Fundamentals of Kinematical X-Ray Scattering Theory

2014 ◽  
pp. 1-14
Keyword(s):  
Scattering Theory ◽  
Profile Analysis ◽  
Reciprocal Lattice ◽  
Line Profile Analysis ◽  
X Ray ◽  
X Ray Scattering ◽  
Basic Concepts ◽  
Lattice Planes ◽  
The Relationship ◽  
Ray Scattering

The broadening of X-ray line profiles is usually described by the kinematical scattering theory. In this chapter, the basic concepts and equations of the kinematical X-ray scattering are presented in order to better understand the theory of line profile analysis. The correlation between the crystal structure and the diffracted intensity distribution is shown. The scattering angles of the diffracted peak maxima are given by the Ewald construction in the reciprocal space. The correspondence between the reciprocal lattice vectors and the lattice planes is also presented, and the relationship between the scattering angle and the lattice plane spacing is given by Bragg’s law.

scholarly journals Salivary sIgA and PRAP-1 Protein in Relation to Dental Caries: A Comparative Study

2020 ◽  
Vol 11 (1) ◽  
pp. 71-76 ◽  
Author(s):  
Aseel Al-ani ◽  
Douglas A. MacDonald ◽  
Maha Ahmad
Keyword(s):  
Dental Caries ◽  
Secretory Iga ◽  
Enzyme Linked Immunosorbent Assay ◽  
Caries Risk ◽  
Confounding Variables ◽  
High Caries Risk ◽  
Caries Management ◽  
Assessment Guidelines ◽  
Unstimulated Saliva ◽  
The Relationship

Aim: Certain individuals are more prone to dental caries than others are. Caries risk may be related in the secretion of certain defensive salivary proteins including secretory IgA (sIgA) and proline-rich acidic protein (PRAP-1). In this study, we investigated the relationship between PRAP-1 and sIgA leading to the susceptibility of development of dental caries in adults and explored the differences in the levels of sIgA and PRAP-1 between men and women. Materials and Methods: Unstimulated saliva samples were collected from 28 patients with high caries risk and 32 control subjects with low caries risk according to caries management by risk assessment guidelines. sIgA and PRAP-1 levels in clarified saliva samples were measured using the enzyme-linked immunosorbent assay. Results: According to our results, sIgA and PRAP-1 levels did not demonstrate statistically significant differences as a function of caries risk or gender, even when potential confounding variables such as age and numbers of teeth were taken into consideration. Estimates of effect size, however, revealed small- to medium-sized effects and suggest that significant results may have been found if larger sample sizes were used. Conclusion: The results of this study indicate that caries risk and salivary levels of sIgA and PRAP-1 do not appear to be significantly associated. Statistically significant findings could emerge if the sample size was larger.

scholarly journals Temporary Relocation during Rest Periods: Relocation Stress and Other Factors Influence Hair Cortisol Concentrations in Horses

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 642
Author(s):  
Jaume Gardela ◽  
Annaïs Carbajal ◽  
Oriol Tallo-Parra ◽  
Sergi Olvera-Maneu ◽  
Manuel Álvarez-Rodríguez ◽  
...  
Keyword(s):  
Hair Growth ◽  
Sudden Change ◽  
Rest Period ◽  
Seasonal Effect ◽  
Hair Cortisol ◽  
Sample Collection ◽  
Rest Periods ◽  
Sport Competition ◽  
Autumn And Winter ◽  
The Relationship

Horse transportation for temporary relocation during rest periods is a common and widespread practice among horse owners, either from sport competition or working tasks. This study aimed to determine the effect of a relocation period and the multiple factors associated with a rest period on hair cortisol concentrations (HCCs) in horses. Additionally, this study reports the seasonal effect on HCCs and hair growth over a year. Thirteen police horses, Pure Spanish stallions of various ages (5–13 y), were selected to participate in this study. Hair sample collection was carried out approximately every 30 d for seven months (Study 1) and a year (Study 2). Cortisol determinations were performed by enzyme immunoassay. Interestingly, Study 1 revealed that relocated horses (n = 4) exhibited elevated HCCs compared with control horses (n = 4) after the relocation period (p < 0.05). Study 2 (n = 5) showed higher HCCs during summer compared with autumn and winter, and higher hair growth rates in winter compared with the other seasons (p < 0.05). Relocated horses had higher HCCs, suggesting a change in their welfare status, probably related to the sudden change in their surrounding conditions. However, these results should be interpreted cautiously due to the low sample size used. The nature of the relationship between HCCs and horse welfare needs to be further examined.

scholarly journals Zonisamide Administration Improves Fatty Acid β-Oxidation in Parkinson’s Disease

Cells ◽  
2018 ◽  
Vol 8 (1) ◽  
pp. 14 ◽  
Author(s):  
Shin-Ichi Ueno ◽  
Shinji Saiki ◽  
Motoki Fujimaki ◽  
Haruka Takeshige-Amano ◽  
Taku Hatano ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Disease Severity ◽  
Rating Scale ◽  
Statistical Significance ◽  
Experimental Studies ◽  
Time Of Flight ◽  
Metabolome Analysis ◽  
Flight Mass Spectrometry

Although many experimental studies have shown the favorable effects of zonisamide on mitochondria using models of Parkinson’s disease (PD), the influence of zonisamide on metabolism in PD patients remains unclear. To assess metabolic status under zonisamide treatment in PD, we performed a pilot study using a comprehensive metabolome analysis. Plasma samples were collected for at least one year from 30 patients with PD: 10 without zonisamide medication and 20 with zonisamide medication. We performed comprehensive metabolome analyses of plasma with capillary electrophoresis time-of-flight mass spectrometry and liquid chromatography time-of-flight mass spectrometry. We also measured disease severity using Hoehn and Yahr (H&Y) staging and the Unified Parkinson’s Disease Rating Scale (UPDRS) motor section, and analyzed blood chemistry. In PD with zonisamide treatment, 15 long-chain acylcarnitines (LCACs) tended to be increased, of which four (AC(12:0), AC(12:1)-1, AC(16:1), and AC(16:2)) showed statistical significance. Of these, two LCACs (AC(16:1) and AC(16:2)) were also identified by partial least squares analysis. There was no association of any LCAC with age, disease severity, levodopa daily dose, or levodopa equivalent dose. Because an upregulation of LCACs implies improvement of mitochondrial β-oxidation, zonisamide might be beneficial for mitochondrial β-oxidation, which is suppressed in PD.

scholarly journals Analysis of Vancomycin-Resistant Enterococci in Hemato-Oncological Patients

Antibiotics ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 785
Author(s):  
Kristýna Hricová ◽  
Taťána Štosová ◽  
Pavla Kučová ◽  
Kateřina Fišerová ◽  
Jan Bardoň ◽  
...  
Keyword(s):  
Surface Protein ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Vancomycin Resistant Enterococci ◽  
Oncological Patients ◽  
Genes Encoding ◽  
Unique Restriction ◽  
Vancomycin Resistant ◽  
Relationship Of ◽  
The Relationship

Enterococci are important bacterial pathogens, and their significance is even greater in the case of vancomycin-resistant enterococci (VRE). The study analyzed the presence of VRE in the gastrointestinal tract (GIT) of hemato-oncological patients. Active screening using selective agars yielded VRE for phenotypic and genotypic analyses. Isolated strains were identified with MALDI-TOF MS, (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry) their susceptibility to antibiotics was tested, and resistance genes (vanA, vanB, vanC-1, vanC2-C3) and genes encoding virulence factors (asa1, gelE, cylA, esp, hyl) were detected. Pulsed-field gel electrophoresis was used to assess the relationship of the isolated strains. Over a period of three years, 103 VanA-type VRE were identified in 1405 hemato-oncological patients. The most frequently detected virulence factor was extracellular surface protein (84%), followed by hyaluronidase (40%). Unique restriction profiles were observed in 33% of strains; clonality was detected in 67% of isolates. The study found that 7% of hemato-oncological patients carried VRE in their GIT. In all cases, the species identified was Enterococcus faecium. No clone persisted for the entire 3-year study period. However, genetically different clusters were observed for shorter periods of time, no longer than eight months, with identical VRE spreading among patients.

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