scholarly journals Global Metabolome Analysis of Dunaliella tertiolecta, Phaeobacter italicus R11 Co-cultures using Thermal Desoprtion - Comprehensive Two-dimensional Gas Chromatography - Time-of-Flight Mass Spectrometry

2021 ◽  
Author(s):  
Michael D S Armstrong ◽  
Oscar René Arredondo Campos ◽  
Catherine C Bannon ◽  
Paulina de la Mata ◽  
Rebecca J Case ◽  
...  
Keyword(s):  
Chemical Nature ◽  
Growth Conditions ◽  
Dunaliella Tertiolecta ◽  
Two Dimensional ◽  
Metabolomic Profile ◽  
Metabolome Analysis ◽  
Flight Mass Spectrometry ◽  
Microalgae Oil ◽  
The Relationship ◽  
Potential Carbon

Dunaliella tertiolecta is a marine microalgae that has been studied extensively as a potential carbon-neutral biofuel source. Microalgae oil contains high quantities of energy-rich fatty acids and lipids, but is not yet commercially viable as an alternative fuel. Carefully optimised growth conditions, and more recently, algal-bacterial co-cultures have been explored as a way of improving the yield of Dunaliella tertiolecta microalgae oils. The relationship between the host microalgae and bacterial co-cultures is currently poorly understood. Here, a complete workflow is proposed to analyse the global metabolomic profile of co-cultured Dunaliella tertiolectra and Phaeobacter italicus R11, which will enable researchers to explore the chemical nature of this relationship in more detail.

scholarly journals Comparison of oral metabolome profiles of stimulated saliva, unstimulated saliva, and mouth-rinsed water

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Yuki Maruyama ◽  
Yuichiro Nishimoto ◽  
Kouta Umezawa ◽  
Ryosuke Kawamata ◽  
Yuko Ichiba ◽  
...  
Keyword(s):  
Profile Analysis ◽  
Saliva Sample ◽  
Biological Information ◽  
Sample Collection ◽  
Metabolome Analysis ◽  
Flight Mass Spectrometry ◽  
Unstimulated Saliva ◽  
Efficient Alternative ◽  
Metabolome Profiling ◽  
The Relationship

AbstractSaliva includes a substantial amount of biological information, which has enabled us to understand the relationship between oral metabolites and various oral and systemic disorders. However, collecting saliva using a controlled protocol is time-consuming, making saliva an unsuitable analyte in large cohort studies. Mouth-rinsed water (MW), the water used to rinse the mouth, can be collected easily in less time with less difference between subjects than saliva and could be used as an alternative in oral metabolome analyses. In this study, we investigated the potential of MW collection as an efficient alternative to saliva sample collection for oral metabolome profiling. MW, stimulated saliva, and unstimulated saliva were collected from 10 systemically healthy participants. The samples were subjected to metabolome analysis using capillary electrophoresis time-of-flight mass spectrometry, and the types and amounts of metabolites in the samples were compared. Qualitatively, MW contained the same metabolites as unstimulated and stimulated saliva. While the quantity of the metabolites did not drastically change between the sampling methods, all three reflected individual differences, and the features of MW were the same as those of the unstimulated saliva. Overall, these results suggest that MW may be an appropriate alternative to saliva in oral metabolome profile analysis.

Analysis of synthetic canine training aids by comprehensive two-dimensional gas chromatography–time of flight mass spectrometry

2012 ◽  
Vol 1255 ◽  
pp. 202-206 ◽  
Author(s):  
Sonja Stadler ◽  
Pierre-Hugues Stefanuto ◽  
Jonathan D. Byer ◽  
Michał Brokl ◽  
Shari Forbes ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Time Of Flight ◽  
Two Dimensional ◽  
Flight Mass Spectrometry ◽  
Training Aids ◽  
Canine Training

scholarly journals Template-Free Self-Assembly of Two-Dimensional Polymers into Nano/Microstructured Materials

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3310
Author(s):  
Shengda Liu ◽  
Jiayun Xu ◽  
Xiumei Li ◽  
Tengfei Yan ◽  
Shuangjiang Yu ◽  
...  
Keyword(s):  
Self Assembly ◽  
Recent Progress ◽  
Two Dimensional ◽  
Subsequent Removal ◽  
The Past ◽  
2D Polymers ◽  
Template Free ◽  
Self Assembled ◽  
The Relationship ◽  
Polymer Tubes

In the past few decades, enormous efforts have been made to synthesize covalent polymer nano/microstructured materials with specific morphologies, due to the relationship between their structures and functions. Up to now, the formation of most of these structures often requires either templates or preorganization in order to construct a specific structure before, and then the subsequent removal of previous templates to form a desired structure, on account of the lack of “self-error-correcting” properties of reversible interactions in polymers. The above processes are time-consuming and tedious. A template-free, self-assembled strategy as a “bottom-up” route to fabricate well-defined nano/microstructures remains a challenge. Herein, we introduce the recent progress in template-free, self-assembled nano/microstructures formed by covalent two-dimensional (2D) polymers, such as polymer capsules, polymer films, polymer tubes and polymer rings.

scholarly journals Paraburkholderia phymatum Homocitrate Synthase NifV Plays a Key Role for Nitrogenase Activity during Symbiosis with Papilionoids and in Free-Living Growth Conditions

Cells ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 952
Author(s):  
Paula Bellés-Sancho ◽  
Martina Lardi ◽  
Yilei Liu ◽  
Sebastian Hug ◽  
Marta Adriana Pinto-Carbó ◽  
...  
Keyword(s):  
Nitrogenase Activity ◽  
Root Nodules ◽  
Growth Conditions ◽  
Lysine Biosynthesis ◽  
Metabolome Analysis ◽  
Plant Host ◽  
Free Living ◽  
Bacterial Enzyme ◽  
Homocitrate Synthase ◽  
Iron Molybdenum Cofactor

Homocitrate is an essential component of the iron-molybdenum cofactor of nitrogenase, the bacterial enzyme that catalyzes the reduction of dinitrogen (N2) to ammonia. In nitrogen-fixing and nodulating alpha-rhizobia, homocitrate is usually provided to bacteroids in root nodules by their plant host. In contrast, non-nodulating free-living diazotrophs encode the homocitrate synthase (NifV) and reduce N2 in nitrogen-limiting free-living conditions. Paraburkholderia phymatum STM815 is a beta-rhizobial strain, which can enter symbiosis with a broad range of legumes, including papilionoids and mimosoids. In contrast to most alpha-rhizobia, which lack nifV, P. phymatum harbors a copy of nifV on its symbiotic plasmid. We show here that P. phymatum nifV is essential for nitrogenase activity both in root nodules of papilionoid plants and in free-living growth conditions. Notably, nifV was dispensable in nodules of Mimosa pudica despite the fact that the gene was highly expressed during symbiosis with all tested papilionoid and mimosoid plants. A metabolome analysis of papilionoid and mimosoid root nodules infected with the P. phymatum wild-type strain revealed that among the approximately 400 measured metabolites, homocitrate and other metabolites involved in lysine biosynthesis and degradation have accumulated in all plant nodules compared to uninfected roots, suggesting an important role of these metabolites during symbiosis.

EQUIVALENCE OF TWO-DIMENSIONAL GRAVITIES

1990 ◽  
Vol 05 (16) ◽  
pp. 1251-1258 ◽  
Author(s):  
NOUREDDINE MOHAMMEDI
Keyword(s):  
Gauge Theory ◽  
Explicit Solution ◽  
Light Cone ◽  
Equations Of Motion ◽  
Two Dimensional ◽  
Induced Gravity ◽  
Light Cone Gauge ◽  
Dimensional Gravity ◽  
The Relationship ◽  
Chern Simons

We find the relationship between the Jackiw-Teitelboim model of two-dimensional gravity and the SL (2, R) induced gravity. These are shown to be related to a two-dimensional gauge theory obtained by dimensionally reducing the Chern-Simons action of the 2+1 dimensional gravity. We present an explicit solution to the equations of motion of the auxiliary field of the Jackiw-Teitelboim model in the light-cone gauge. A renormalization of the cosmological constant is also given.

“Beholding the Man”: Viewing (or is it Marking?) John’s Trial Scene alongside Kitsch Art

2016 ◽  
Vol 24 (2) ◽  
pp. 245-264
Author(s):  
Andrew P. Wilson
Keyword(s):  
Two Dimensional ◽  
Devotional Art ◽  
The People ◽  
Subject And Object ◽  
Ecce Homo ◽  
Small Stories ◽  
John's Gospel ◽  
Relationship Of ◽  
The Relationship ◽  
Point Of Departure

One of the grand scenes of the Passion narratives can be found in John’s Gospel where Pilate, presenting Jesus to the people, proclaims “Behold the man”: “Ecce Homo.” But what exactly does Pilate mean when he asks the reader to “Behold”? This paper takes as its point of departure a roughly drawn picture of Jesus in the “Ecce Homo” tradition and explores the relationship of this picture to its referent in John’s Gospel, via its capacity as kitsch devotional art. Contemporary scholarship on kitsch focuses on what kitsch does, or how it functions, rather than assessing what it is. From this perspective, when “beholding” is understood not for what it reveals but for what it does, John’s scene takes on a very different significance. It becomes a scene that breaks down traditional divisions between big and small stories, subject and object as well as text and context. A kitsch perspective opens up possibilities for locating John’s narrative in unexpected places and experiences. Rather than being a two-dimensional departure from the grandeur of John’s trial scene, kitsch “art” actually provides a lens through which the themes and dynamics of the narrative can be re-viewed with an expansiveness somewhat lacking from more traditional commentary.


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