scholarly journals De-novo fabrication of sunlight irradiated silver nanoparticles and their efficacy against E. coli and S. epidermidis

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Hammad Arshad ◽  
Saima Sadaf ◽  
Umer Hassan
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
De Novo ◽  
Cost Effective ◽  
Microbial Pathogens ◽  
Solar Irradiation ◽  
Root Extract ◽  
Salvadora Persica ◽  
Tem Analysis ◽  
E Coli

AbstractSilver nanoparticles (AgNPs) gained significant attention due to their activity against microbial pathogens, cancer cells, and viral particles etc. Traditional fabrication methods require hazardous chemicals as reducing agents and their usage and disposal pose a significant hazard to environmental ecosystem. Here, a de novo, robust, cost effective and an eco-friendly method is reported to fabricate AgNPs irradiated with sunlight (SL) while using Salvadora persica root extract (SPE) as reducing agent. Sunlight (SL) irradiated S. persica silver nanoparticles (SpNPs) i.e., SL-SpNPs were characterized using multiple techniques and their antibacterial efficacy was evaluated. The SL-SpNPs were synthesized in 10 min. Field emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM) analysis revealed their spherical morphology with a size range of 4.5–39.7 nm, while surface plasmon resonance (SPR) peaked at 425 nm. Fourier transform infrared spectroscopy (FTIR) analysis suggested that the reduction of SL-SpNPs was due to the presence of phytochemicals in the SPE. Furthermore, X-ray powder diffraction (P-XRD) pattern depicted the crystal structure of SL-SpNPs, hence proving the presence of AgNPs. Further the antibacterial studies were carried out against Escherichia coli (ATCC 11229) and Staphylococcus epidermidis (ATCC 12228) using Kirby Bauer method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for E. coli were determined to be 1.5 μg/mL and 3.0 μg/mL respectively while MIC and MBC values for S. epidermidis were found to be 12.5 μg/mL and 25 μg/mL respectively. The solar irradiation-based fabrication method and resulting SL-SpNPs can find their utility in many biomedical and environmental applications.

scholarly journals PSIDIUM GUAJAVA: A NOVEL PLANT IN THE SYNTHESIS OF SILVER NANOPARTICLES FOR BIOMEDICAL APPLICATIONS.

2018 ◽  
Vol 11 (1) ◽  
pp. 341
Author(s):  
Sharmila C ◽  
Ranjith Kumar R ◽  
Chandar Shekar B
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Biomedical Applications ◽  
Leaf Extract ◽  
Cost Effective ◽  
Psidium Guajava ◽  
Tem Analysis ◽  
X Ray ◽  
Green Route

 Objective: Synthesis of silver nanoparticles (AgNPs) using a simple, cost-effective and environmentally friendly green route approach and to study the antibacterial activity of AgNPs against human pathogens.Methods: Green route approach is used to synthesize AgNPs using Psidium guajava leaf extract. Fourier transform infrared (FTIR) was used to identify the presence of the functional group. X-ray diffraction (XRD) was used to analyze the structure of prepared AgNPs. Energy dispersive X-ray was used to the characteristic to the composition of the prepared nanoparticles. Size and morphology of the prepared AgNPs were investigated using field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) analysis. Antibacterials efficiency of prepared AgNPs was tested against Escherichia coli and Staphylococcus aureus by well diffusion methods.Results: FTIR study shows the presence of different functional groups present in the leaves mediated AgNPs. The XRD studies yield diffraction peaks corresponding to face-centered cubic structure of Ag crystals. Spherical shaped AgNPs with a particle size of about ~55 nm were evidenced using FESEM and TEM analysis. Energy dispersive spectrum of the synthesized AgNPs confirms the presence of silver in the prepared nanoparticles. From UV-VIS analysis it is shown that the absorption band was red-shifted from 430 nm to 456 nm. The prepared AgNPs shows good antibacterial activity against E. coli and S. aureus.Conclusions: P. guajava leaf extract is a potential reducing agent to synthesize AgNPs. The green synthesis approach provides cost-effective and eco-friendly nanoparticles, which could be used in biomedical applications.

scholarly journals PSIDIUM GUAJAVA: A NOVEL PLANT IN THE SYNTHESIS OF SILVER NANOPARTICLES FOR BIOMEDICAL APPLICATIONS.

2018 ◽  
Vol 11 (1) ◽  
pp. 341 ◽  
Author(s):  
Sharmila C ◽  
Ranjith Kumar R ◽  
Chandar Shekar B
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Biomedical Applications ◽  
Leaf Extract ◽  
Cost Effective ◽  
Psidium Guajava ◽  
Tem Analysis ◽  
X Ray ◽  
Green Route

 Objective: Synthesis of silver nanoparticles (AgNPs) using a simple, cost-effective and environmentally friendly green route approach and to study the antibacterial activity of AgNPs against human pathogens.Methods: Green route approach is used to synthesize AgNPs using Psidium guajava leaf extract. Fourier transform infrared (FTIR) was used to identify the presence of the functional group. X-ray diffraction (XRD) was used to analyze the structure of prepared AgNPs. Energy dispersive X-ray was used to the characteristic to the composition of the prepared nanoparticles. Size and morphology of the prepared AgNPs were investigated using field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) analysis. Antibacterials efficiency of prepared AgNPs was tested against Escherichia coli and Staphylococcus aureus by well diffusion methods.Results: FTIR study shows the presence of different functional groups present in the leaves mediated AgNPs. The XRD studies yield diffraction peaks corresponding to face-centered cubic structure of Ag crystals. Spherical shaped AgNPs with a particle size of about ~55 nm were evidenced using FESEM and TEM analysis. Energy dispersive spectrum of the synthesized AgNPs confirms the presence of silver in the prepared nanoparticles. From UV-VIS analysis it is shown that the absorption band was red-shifted from 430 nm to 456 nm. The prepared AgNPs shows good antibacterial activity against E. coli and S. aureus.Conclusions: P. guajava leaf extract is a potential reducing agent to synthesize AgNPs. The green synthesis approach provides cost-effective and eco-friendly nanoparticles, which could be used in biomedical applications.

scholarly journals Salvadora persica mediated synthesis of silver nanoparticles and their antimicrobial efficacy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hammad Arshad ◽  
Muhammad A. Sami ◽  
Saima Sadaf ◽  
Umer Hassan
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antimicrobial Properties ◽  
Nano Particles ◽  
Antimicrobial Efficacy ◽  
Root Extract ◽  
Bacterial Cells ◽  
Salvadora Persica ◽  
Nitrogenous Compounds ◽  
Vis Spectroscopy

AbstractSilver nanoparticles (AgNPs) exhibit strong antimicrobial properties against many pathogens. Traditionally employed chemical methods for AgNPs synthesis are toxic for the environment. Here, we report a quicker, simpler, and environmentally benign process to synthesize AgNPs by using an aqueous ‘root extract’ of Salvadora persica (Sp) plant as a reducing agent. The synthesized Salvadora persica nano particles (SpNPs) showed significantly higher antimicrobial efficacy compared to earlier reported studies. We characterized SpNPs using UV–Vis spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), Transmission Electron Microscopy (TEM), Field Emission Scanning Electron Microscopy (FE-SEM), Dynamic Light Scattering (DLS) and X-ray powder diffraction (P-XRD). UV–Vis spectrum showed the highest absorbance at 420 nm. FTIR analysis depicts presence of bond stretching including OH– (3300 cm−1), C=N– (2100 cm−1) and NH– (1630 cm−1) which are attributed in the involvement of phenolics, proteins or nitrogenous compounds in reduction and stabilization of AgNPs. TEM, FE-SEM and DLS analysis revealed the spherical and rod nature of SpNPs and an average size of particles as 37.5 nm. XRD analysis showed the presence of the cubic structure of Ag which confirmed the synthesis of silver nanoparticles. To demonstrate antimicrobial efficacy, we evaluated SpNPs antimicrobial activity against two bacterial pathogens (Escherichia coli (ATCC 11229) and Staphylococcus epidermidis (ATCC 12228)). SpNPs showed a significantly high inhibition for both pathogens and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were found to be 0.39 µg/mL and 0.78 µg/mL for E. coli while 0.19 µg/mL and 0.39 µg/mL for S. epidermidis respectively. Further, Syto 16 staining of bacterial cells provided a supplemental confirmation of the antimicrobial efficacy as the bacterial cells treated with SpNPs stop to fluoresce compared to the untreated bacterial cells. Our highly potent SpNPs will likely have a great potential for many antimicrobial applications including wound healing, water purification, air filtering and other biomedical applications.

scholarly journals Optimization of biogenic synthesis of silver nanoparticles from flavonoid-rich Clinacanthus nutans leaf and stem aqueous extracts

2020 ◽  
Vol 7 (7) ◽  
pp. 200065 ◽  
Author(s):  
Siti Nur Aishah Mat Yusuf ◽  
Che Nurul Azieyan Che Mood ◽  
Nor Hazwani Ahmad ◽  
Doblin Sandai ◽  
Chee Keong Lee ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Functional Groups ◽  
Silver Ions ◽  
Cost Effective ◽  
Aqueous Extracts ◽  
Tem Analysis ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
The Face ◽  
Clinacanthus Nutans

Background : Silver nanoparticles (AgNPs) are widely used in food industries, biomedical, dentistry, catalysis, diagnostic biological probes and sensors. The use of plant extract for AgNPs synthesis eliminates the process of maintaining cell culture and the process could be scaled up under a non-aseptic environment. The purpose of this study is to determine the classes of phytochemicals, to biosynthesize and characterize the AgNPs using Clinacanthus nutans leaf and stem extracts. In this study, AgNPs were synthesized from the aqueous extracts of C. nutans leaves and stems through a non-toxic, cost-effective and eco-friendly method. Results : The formation of AgNPs was confirmed by UV-Vis spectroscopy, and the size of AgNP-L (leaf) and AgNP-S (stem) were 114.7 and 129.9 nm, respectively. Transmission electron microscopy (TEM) analysis showed spherical nanoparticles with AgNP-L and AgNP-S ranging from 10 to 300 nm and 10 to 180 nm, with average of 101.18 and 75.38 nm, respectively. The zeta potentials of AgNP-L and AgNP-S were recorded at −42.8 and −43.9 mV. X-ray diffraction analysis matched the face-centred cubic structure of silver and was capped with bioactive compounds. Fourier transform infrared spectrophotometer analysis revealed the presence of few functional groups of phenolic and flavonoid compounds. These functional groups act as reducing agents in AgNPs synthesis. Conclusion : These results showed that the biogenically synthesized nanoparticles reduced silver ions to silver nanoparticles in aqueous condition and the AgNPs formed were stable and less toxic.

Application of Laser Deprocessing Techniques in Physical Failure Analysis

2013 ◽  
Author(s):  
H.H. Yap ◽  
P.K. Tan ◽  
J. Lam ◽  
T.H. Ng ◽  
G.R. Low ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Failure Analysis ◽  
Cost Effective ◽  
Large Area ◽  
Top Down ◽  
Tem Analysis ◽  
Defect Identification ◽  
Nanometer Range ◽  
Transmission Electron

Abstract With the scaling of semiconductor devices to nanometer range, ensuring surface uniformity over a large area while performing top down physical delayering has become a greater challenge. In this paper, the application of laser deprocessing technique (LDT) to achieve better surface uniformity as well as for fast deprocessing of sample for defect identification in nanoscale devices are discussed. The proposed laser deprocess technique is a cost-effective and quick way to deprocess sample for defect identification and Transmission Electron Microscopy (TEM) analysis.

Titanium nanoparticles phytosynthesized from Eichhornia crassipes leaf extract and their antimicrobial activity.

MRS Advances ◽  
2020 ◽  
Vol 5 (62) ◽  
pp. 3293-3299
Author(s):  
Monserrat Velázquez-Hernández ◽  
Pablo Schabes-Retchkiman ◽  
Sonia Martínez-Gallegos ◽  
V. Albiter
Keyword(s):  
Electron Microscopy ◽  
Antimicrobial Activity ◽  
Eichhornia Crassipes ◽  
Leaf Extract ◽  
Plate Count ◽  
Tem Analysis ◽  
E Coli ◽  
Solid Agar ◽  
Bacteriological Test ◽  
Titanium Nanoparticles

AbstractIn this study, we reported an environmentally friendly technique for the synthesis of titanium nanoparticles using Eichhornia crassipes leaf extract as a non-toxic reducing agent and efficient stabilizer. Also the antimicrobial activity TiNPs against E. coli. Bacteriological test were performed on solid agar plates with different concentrations of TiNPs. On the other hand TiNPs were characterized by UV-visible spectroscopy, EDS (Dispersive Energy X-ray Spectroscopy), SEM (Scanning Electron Microscopy), and TEM (Transmission Electron Microscopy). It was found that TiNPs exhibit high crystallinity whit rutile titania structure. TEM analysis shows the nanoparticle size in the range from 22 to 44 nm, antimicrobial study was performed by plate count technique witch showed >99% mortality for E. coli bacteria studied after 24 h of incubation.

scholarly journals Green Synthesis of Silver Nanoparticles Using Astragalus tribuloides Delile. Root Extract: Characterization, Antioxidant, Antibacterial, and Anti-Inflammatory Activities

Nanomaterials ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2383
Author(s):  
Majid Sharifi-Rad ◽  
Pawel Pohl ◽  
Francesco Epifano ◽  
José M. Álvarez-Suarez
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Total Phenolics ◽  
Cost Effective ◽  
Root Extract ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
High Efficient ◽  
Anti Inflammatory ◽  
Average Size

Today, the green synthesis of metal nanoparticles is a promising strategy in material science and nanotechnology. In this research, silver nanoparticles (AgNPs) were synthesized through the high-efficient, cost-effective green and facile process, using the Astragalus tribuloides Delile. root extract as a bioreduction and capping agent at room temperature. UV–Vis spectroscopy was applied for the investigation of the reaction proceedings. To characterize the greenly synthesized AgNPs, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and transmission electron microscopy (TEM) analyses were utilized. In addition, the total phenolics and flavonoids contents, antioxidant, antibacterial, and anti-inflammatory activities of the greenly synthesized AgNPs and the A. tribuloides root extract were evaluated. The results indicated that the AgNPs had spherical morphology and crystalline structure with the average size of 34.2 ± 8.0 nm. The total phenolics and flavonoids contents of the greenly synthesized AgNPs were lower than those for the A. tribuloides root extract. The resultant AgNPs exhibited the appropriate antioxidant activity (64%) as compared to that for the A. tribuloides root extract (47%). The antibacterial test approved the higher bactericidal activity of the resulting AgNPs on the Gram-positive and Gram-negative bacteria in comparison to the A. tribuloides root extract. Considering the anti-inflammatory activity, the greenly synthesized AgNPs showed a stranger effect than the A. tribuloides root extract (82% versus 69% at 500 μg/mL). Generally, the AgNPs that were fabricated by using the A. tribuloides root extract had appropriate antioxidant, antibacterial, and anti-inflammatory activities and, therefore, can be considered as a promising candidate for various biomedical applications.

scholarly journals Biological Synthesis of Silver Nanoparticles by Cell-Free Extract ofSpirulina platensis

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Gaurav Sharma ◽  
Nakuleshwar Dut Jasuja ◽  
Manoj Kumar ◽  
Mohammad Irfan Ali
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Large Scale ◽  
Cost Effective ◽  
Biological Synthesis ◽  
Cell Free Extract ◽  
Tem Analysis ◽  
High Antibacterial Activity ◽  
Vis Spectroscopy ◽  
Average Size

The present study explores biological synthesis of silver nanoparticles (AgNPs) using the cell-free extract ofSpirulina platensis. Biosynthesised AgNPs were characterised by UV-Vis spectroscopy, SEM, TEM, and FTIR analysis and finally evaluated for antibacterial activity. Extracellular synthesis using aqueous extract ofS. platensisshowed the formation of well scattered, highly stable, spherical AgNPs with an average size of 30–50 nm. The size and morphology of the nanoparticles were confirmed by SEM and TEM analysis. FTIR and UV-Vis spectra showed that biomolecules, proteins and peptides, are mainly responsible for the formation and stabilisation of AgNPs. Furthermore, the synthesised nanoparticles exhibited high antibacterial activity against pathogenic Gram-negative, that is,Escherichia coli, MTCC-9721;Proteus vulgaris, MTCC-7299;Klebsiella pneumoniae, MTCC-9751, and Gram-positive, that is,Staphylococcus aureus, MTCC-9542;S. epidermidis, MTCC-2639;Bacillus cereus, MTCC-9017, bacteria. The AgNPs had shown maximum zone of inhibition (ZOI) that is31.3±1.11inP. vulgaris. Use of such a microalgal system provides a simple, cost-effective alternative template for the biosynthesis of nanomaterials of silver in a large scale that could be of great use in biomedical applications.

Trillium govanianum Wall. Ex. Royle rhizomes extract-medicated silver nanoparticles and their antimicrobial activity

2020 ◽  
Vol 9 (1) ◽  
pp. 503-514 ◽  
Author(s):  
Khaleeq Uz-Zaman ◽  
Jehan Bakht ◽  
Bates Kudaibergenova Malikovna ◽  
Eman R. Elsharkawy ◽  
Anees Ahmed Khalil ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cost Effective ◽  
Face Centered Cubic ◽  
Synthesis Of Nanoparticles ◽  
E Coli ◽  
Area Of Interest ◽  
Cost Effective Technique ◽  
Face Centered ◽  
Trillium Govanianum ◽  
Average Size

AbstractSynthesis of nanoparticles is a fast-growing area of interest in the current development in science and technology. Nanoparticles are also used in biomedical applications. Green synthesis of nanoparticles is an environmental friendly and cost-effective technique. Trillium govanianum Wall. Ex. Royle crude extract was used for the eco-friendly genesis of silver nanoparticles (AgNPs). Aromatic amines were the functional groups involved in the bio-fabrication and synthesis of the AgNPs. The production of AgNPs was established by the appearance of brown color. The manufactured AgNPs were characterized by UV-Vis spectrophotometer, X-ray diffractometer, and FTIR spectrophotometer. AgNPs were face-centered cubic in nature with an average size of 9.99 nm. The produced AgNPs (18 µL disc−1) showed substantial antibacterial (53.74, 52.75, 51.61, 43.00, 36.84, and 36.84%) and antifungal (54.05, 42.11, 41.10, 40.85, 30.55, and 29.73%) potential against the tested bacterial (X. campestris, P. aeruginosa, S. aureus, E. coli, B. subtilis, and K. pneumoniae) and fungal (A. alternaria, Paecilomyces, C. albicans, Curvularia, A. niger, and Rhizopus) strains, respectively.

scholarly journals Bacterial Mediated Rapid and Facile Synthesis of Silver Nanoparticles and Their Antimicrobial Efficacy against Pathogenic Microorganisms

Materials ◽  
2021 ◽  
Vol 14 (10) ◽  
pp. 2615
Author(s):  
Md. Amdadul Huq ◽  
Shahina Akter
Keyword(s):  
Electron Microscopy ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Field Emission ◽  
Spherical Shape ◽  
Xrd Analysis ◽  
Gram Positive ◽  
Tem Analysis ◽  
Gram Negative ◽  
X Ray

In the present study, silver nanoparticles (AgNPs), biosynthesized using culture supernatant of bacterial strain Paenarthrobacter nicotinovorans MAHUQ-43, were characterized and their antimicrobial activity was investigated against both Gram-positive Bacillus cereus and Gram-negative bacteria Pseudomonas aeruginosa. Bacterial-mediated synthesized AgNPs were characterized by UV-Visible (UV-Vis) spectrophotometer, field emission-transmission electron microscopy (FE-TEM), energy dispersive X-ray (EDX), X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and dynamic light scattering (DLS) analysis. The UV-Vis spectral analysis showed the absorption maxima at 466 nm which assured the synthesis of AgNPs. The FE-TEM analysis revealed the spherical shape of nanoparticles with the size range from 13 to 27 nm. The EDX and XRD analysis ensured the crystalline nature of biosynthesized AgNPs. The FTIR analysis revealed the involvement of different biomolecules for the synthesis of AgNPs as reducing and capping agents. The bacterial-mediated synthesized AgNPs inhibited the growth of pathogenic strains B. cereus and P. aeruginosa and developed a clear zone of inhibition (ZOI). The MIC and MBC for both pathogens were 12.5 µg/mL and 25 µg/mL, respectively. Moreover, field emission scanning electron microscopy analysis revealed that the synthesized AgNPs can destroy the outer membrane and alter the cell morphology of treated pathogens, leading to the death of cells. This study concludes the eco-friendly, facile and rapid synthesis of AgNPs using P. nicotinovorans MAHUQ-43 and synthesized AgNPs showed excellent antimicrobial activity against both Gram-positive and Gram-negative pathogens.

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