scholarly journals Lysosome repositioning as an autophagy escape mechanism by Mycobacterium tuberculosis Beijing strain

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Thanida Laopanupong ◽  
Pinidphon Prombutara ◽  
Phongthon Kanjanasirirat ◽  
Salisa Benjaskulluecha ◽  
Atsadang Boonmee ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Reference Strain ◽  
Beijing Genotype ◽  
Cell Periphery ◽  
Beijing Strain ◽  
Perinuclear Region ◽  
Rna Seq ◽  
Autophagy Induction ◽  
Infected Cells ◽  
Strain H37rv

AbstractInduction of host cell autophagy by starvation was shown to enhance lysosomal delivery to mycobacterial phagosomes, resulting in the restriction of Mycobacterium tuberculosis reference strain H37Rv. Our previous study showed that strains belonging to M. tuberculosis Beijing genotype resisted starvation-induced autophagic elimination but the factors involved remained unclear. Here, we conducted RNA-Seq of macrophages infected with the autophagy-resistant Beijing strain (BJN) compared to macrophages infected with H37Rv upon autophagy induction by starvation. Results identified several genes uniquely upregulated in BJN-infected macrophages but not in H37Rv-infected cells, including those encoding Kxd1 and Plekhm2, which function in lysosome positioning towards the cell periphery. Unlike H37Rv, BJN suppressed enhanced lysosome positioning towards the perinuclear region and lysosomal delivery to its phagosome upon autophagy induction by starvation, while depletion of Kxd1 and Plekhm2 reverted such effects, resulting in restriction of BJN intracellular survival upon autophagy induction by starvation. Taken together, these data indicated that Kxd1 and Plekhm2 are important for the BJN strain to suppress lysosome positioning towards the perinuclear region and lysosomal delivery into its phagosome during autophagy induction by starvation to evade starvation-induced autophagic restriction.

scholarly journals Fatal congenital tuberculosis due to a Beijing strain in a premature neonate

2010 ◽  
Vol 59 (6) ◽  
pp. 733-735 ◽  
Author(s):  
M. L. Abalain ◽  
O. Petsaris ◽  
G. Héry-Arnaud ◽  
P. Marcorelles ◽  
F. Couturaud ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Early Detection ◽  
Premature Neonate ◽  
Beijing Genotype ◽  
Beijing Strain ◽  
Antituberculous Therapy ◽  
Rapid Improvement ◽  
Specific Nature ◽  
Congenital Tuberculosis ◽  
Urine Specimens

Congenital tuberculosis (TB) remains a rare disease but is fatal if untreated. Early detection is difficult because of the non-specific nature of the symptoms in TB during pregnancy and infancy. This report summarizes a case of congenital TB in a very premature infant, born at 25 weeks gestation. Miliary TB was diagnosed in the mother when the neonate was 20 days old. Antituberculous therapy allowed a rapid improvement in the mother. The infant died at 27 days old. A Beijing genotype strain of Mycobacterium tuberculosis was isolated both in the mother, from pulmonary and urine specimens, and in the infant, from peritoneal fluid.

scholarly journals Differences in Rate and Variability of Intracellular Growth of a Panel of Mycobacterium tuberculosis Clinical Isolates within a Human Monocyte Model

2002 ◽  
Vol 70 (11) ◽  
pp. 6489-6493 ◽  
Author(s):  
Qing Li ◽  
Christopher C. Whalen ◽  
Jeffrey M. Albert ◽  
Rhonda Larkin ◽  
Lynn Zukowski ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Human Monocyte ◽  
Clinical Isolates ◽  
Beijing Strain ◽  
Human Monocytes ◽  
Intracellular Growth ◽  
Strain Family ◽  
Strain H37rv

ABSTRACT Significant differences were observed in the capacities of Mycobacterium tuberculosis clinical isolates to grow within human monocytes. Genotyping indicated that the four most rapidly growing isolates were members of the Beijing strain family. M. tuberculosis strain H37Rv provided more reproducible infection than the clinical isolates or M. tuberculosis Erdman.

Digital RNA-seq transcriptome plus tissue anatomy analyses reveal the developmental mechanism of the calabash-shaped root in Tetrastigma hemsleyanum

2021 ◽  
Author(s):  
Taihe Xiang ◽  
Jiangshan Li ◽  
Shuying Bao ◽  
Zhengxian Xu ◽  
Leizhen Wang ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Chinese Herb ◽  
Acc Oxidase ◽  
Mapk Signaling ◽  
Cell Periphery ◽  
Vascular Bundles ◽  
Rna Seq ◽  
Short Root ◽  
Tetrastigma Hemsleyanum ◽  
Intrinsic Component

Abstract Tetrastigma hemsleyanum is a liana plant with promising medicinal and ornamental values. Its calabash-shaped roots (CRs) are served as a traditional Chinese herb. However, it takes a long growth period to form CRs. In the present study, three types of architectural roots, including fine roots (FRs), bar-shaped roots (BRs) and CRs, were employed as materials, and the characteristics of histo-anatomy and digital RNA-seq transcriptome profiles were analyzed. Among the three types of roots, the vascular bundles in FRs were intact, while some of the vascular bundles degenerated in BRs, and only few traces of vascular bundles existed in CRs. Meanwhile, no obvious cell inclusions were found in the cytoplasm of FRs, while a few inclusions were found in BRs, and abundant inclusions were detected in CRs, which might be the main source of medicinal components in roots. The transcriptome profiles and qRT-PCR validation indicated that seven up-regulated genes encoding xyloglucan glycosyltransferase, ACC oxidase, CYP711A1, SHORT-ROOT transcript factor, galacturonosyltransferas, WAT1 and WRKY, and two down-regulated genes encoded LRR receptor-like serine/threonine-protein kinase and CYP83B1, were probably involved in the formation and development of CRs. Besides, GO terms of intrinsic component of membrane, integral component of membrane, cell periphery, membrane part, plasma membrane, membrane, intrinsic component of plasma membrane, cellular chemical homeostasis, and plasma membrane part were probably related to the formation of CRs. KEGG pathways related to the development of CRs probably included MAPK signaling pathway-plant, plant hormone signal transduction, and circadian rhythm-plant. Our finding suggested a probable mode for the formation of CRs.

Mutations in the regulatory network underlie the recent clonal expansion of a dominant subclone of the Mycobacterium tuberculosis Beijing genotype

2011 ◽  
Vol 11 (3) ◽  
pp. 587-597 ◽  
Author(s):  
Anita C. Schürch ◽  
Kristin Kremer ◽  
Robin M. Warren ◽  
Nguyen V. Hung ◽  
Yanlin Zhao ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Regulatory Network ◽  
Clonal Expansion ◽  
Beijing Genotype

scholarly journals HIV-1 Infection Transcriptomics: Meta-Analysis of CD4+ T Cells Gene Expression Profiles

Viruses ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 244 ◽  
Author(s):  
Antonio Victor Campos Coelho ◽  
Rossella Gratton ◽  
João Paulo Britto de Melo ◽  
José Leandro Andrade-Santos ◽  
Rafael Lima Guimarães ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cells ◽  
Differentially Expressed Genes ◽  
Cd4 T Cells ◽  
Expression Profiles ◽  
Meta Analysis ◽  
Differentially Expressed ◽  
Rna Seq ◽  
Infected Cells ◽  
Hiv 1

HIV-1 infection elicits a complex dynamic of the expression various host genes. High throughput sequencing added an expressive amount of information regarding HIV-1 infections and pathogenesis. RNA sequencing (RNA-Seq) is currently the tool of choice to investigate gene expression in a several range of experimental setting. This study aims at performing a meta-analysis of RNA-Seq expression profiles in samples of HIV-1 infected CD4+ T cells compared to uninfected cells to assess consistently differentially expressed genes in the context of HIV-1 infection. We selected two studies (22 samples: 15 experimentally infected and 7 mock-infected). We found 208 differentially expressed genes in infected cells when compared to uninfected/mock-infected cells. This result had moderate overlap when compared to previous studies of HIV-1 infection transcriptomics, but we identified 64 genes already known to interact with HIV-1 according to the HIV-1 Human Interaction Database. A gene ontology (GO) analysis revealed enrichment of several pathways involved in immune response, cell adhesion, cell migration, inflammation, apoptosis, Wnt, Notch and ERK/MAPK signaling.

scholarly journals Increased Transmission of Mycobacterium tuberculosis Beijing Genotype Strains Associated with Resistance to Streptomycin: A Population-Based Study

PLoS ONE ◽  
2012 ◽  
Vol 7 (8) ◽  
pp. e42323 ◽  
Author(s):  
Tran N. Buu ◽  
Dick van Soolingen ◽  
Mai N. T. Huyen ◽  
Nguyen T. N. Lan ◽  
Hoang T. Quy ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Population Based ◽  
Beijing Genotype ◽  
Population Based Study

scholarly journals In vivo virulence of Beijing genotype Mycobacterium tuberculosis

2019 ◽  
Vol 9 (1) ◽  
pp. 173-182 ◽  
Author(s):  
J. A. Bespyatykh ◽  
Т. I. Vinogradova ◽  
O. A. Manicheva ◽  
N. V. Zabolotnykh ◽  
M. Z. Dogonadze ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Beijing Genotype

scholarly journals Black Cumin (Nigella sativa) Against Mycobacterium tuberculosis Strain H37RV And MDR-TB

Elkawnie ◽  
2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Mashuri Masri ◽  
Cut Muthiadin ◽  
Masita Masita ◽  
Tri Cahyanto ◽  
Lianah Lianah ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Multidrug Resistance ◽  
Microscopic Observation ◽  
Nigella Sativa ◽  
Drug Susceptibility ◽  
Tuberculosis Strain ◽  
Black Cumin ◽  
Mdr Tb ◽  
Susceptibility Assay ◽  
Strain H37rv

Abstract: Tuberculosis (TB) is a contagious infectious disease caused by Mycobacterium tuberculosis. 10 million people suffer from TB Every year. Although TB is a preventable and treatable disease, 1.5 million people die every year due to TB. Alternative treatments continue to be pursued, and treatment with the latest TB drugs that are continuously being encouraged. Black cumin (Nigella sativa) seed contains essential oils with active compounds such as thymohydroquinone, Oleoresins, flavonoids, alkaloids, saponins, tannins, and terpenoids that act as antibacterial drugs. This study aims to determine the sensitivity of  N. sativa seed extract in inhibiting the growth of  M. tuberculosis strain H37RV and MDR-TB (Multidrug Resistance-TB). This research using Microscopic-Observation and Drug-Susceptibility Assay (MODS) method. Extraction of N. sativa was carried out by the maceration method using 70% methanol as a solvent. The results showed that the M. tuberculosis strain H37RV and MDR-TB were sensitive to N. sativa extract at concentrations of 5 and 10% but resistant to N. sativa extract at concentrations of 1 and 3%.Abstrak: Tuberkulosis (TB) adalah penyakit menular yang disebabkan oleh Bakteri Mycobacterium tuberculosis. Penyakit ini menimbulkan dampak kematian yang cukup mengkhawatirkan.  Penyakit tersebut dapat dicegah dan diobati. Salah satu sumber pengobatannya menggunakan biji jintan hitam (Nigella sativa) yang mengandung minyak atsiri dengan senyawa aktif seperti timohidrokuinon, oleoresin, flavonoid, alkaloid, saponin, tanin, dan terpenoid yang berfungsi sebagai obat antibakteri. Penelitian ini bertujuan untuk mengetahui sensitivitas ekstrak biji N. sativa dalam menghambat pertumbuhan M. tuberculosis strain H37RV and MDR-TB (Multidrug-Resistance-TB). Penelitian ini menggunakan metode Microscopic-Observation and Drug-Susceptibility Assay (MODS). Ekstraksi N. sativa dilakukan dengan metode maserasi menggunakan pelarut metanol 70%. Hasil yang diperoleh menunjukkan bahwa bakteri M. tuberculosis strain H37RV dan TB-MDR, kedua  strain tsb sensitif terhadap ekstrak N. sativa konsentrasi 5 dan 10%,  tetapi resisten terhadap  ekstrak N. sativa konsentrasi 1 dan 3%.

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