scholarly journals High particle variability across siliconized and oil-free syringes and needles from the same lots

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Lydianne Lumack do Monte Agra ◽  
Natasha Ferreira Santos da Cruz ◽  
Vaida Linkuviene ◽  
John F. Carpenter ◽  
Michel Eid Farah ◽  
...  
Keyword(s):  
Primary Outcome ◽  
Silicone Oil ◽  
Flow Imaging ◽  
Becton Dickinson ◽  
Significance Level ◽  
Particle Release ◽  
High Particle ◽  
Coefficients Of Variation ◽  
Miller Test ◽  
Number Of Particles

AbstractPrevious studies have reported silicone oil (SO) applied to needles and syringes in the vitreous of patients after intravitreal injections. We evaluated four syringes (SR 1-mL insulin, Saldanha-Rodrigues; BD 1-mL Tuberculin Slip Tip, Becton–Dickinson; BD Ultra-Fine 0.3 mL, HSW Norm-Ject Tuberculin, Henke Sass Wolf) and 10 needles (BD PrecisionGlide 27- and 30-gauge (G); BD Eclipse and JBP Nanoneedle 27-, 30-, 33- and 34-G; TSK Invisible Needle and 27 and 30-G Steriject Control Hub). The protein-free buffer samples injected into the syringes and needles under study were collected in an Eppendorf tube and taken to Flow imaging microscopy, that characterized the concentration and morphology of the microsized particles. The number of particles was analyzed. The coefficients of variation (CV) were the primary outcome. The Feltz and Miller test compared the CVs. The significance level was 5%. Numerous particles and high CVs were associated with both devices, needles and syringes; the comparisons among them did not reach significance. The BD Ultrafine 0.3 mL syringe (149.7%) had the highest CV and the SO-free HSW Norm-Ject (66.4%) syringe the lowest, and the TSK Invisible needle (149.5%) had the highest and the BD Precision Glide 30G needle (35.9%) needle the lowest. In conclusion, particle release, including those with SO morphology, varied greatly among instruments, even from the same lots, which is relevant considering that fewer particles are injected into some eyes compared with others.

Gas Chromatographic Determination of Dioxathion and Chlorfenvinphos in Emulsifiable Formulations and Livestock Dips

1970 ◽  
Vol 53 (3) ◽  
pp. 566-568
Author(s):  
J E Paterson
Keyword(s):  
Quantitative Determination ◽  
Chromatographic Analysis ◽  
Chromatographic Method ◽  
Silicone Oil ◽  
Chromatographic Determination ◽  
Ionization Detector ◽  
Flame Ionization ◽  
Coefficients Of Variation ◽  
Gas Chromatographic Method

Abstract A gas chromatographic method is described for the quantitative determination of dioxathion and chlorfenvinphos in emulsifiable concentrates and livestock dips. A convenient quantity of the emulsifiable formulation is dissolved in xylene and the aqueous dip is extracted with xylene for gas chromatographic analysis, using a mixed silicone oil stationary phase and a flame ionization detector. The coefficients of variation for the dioxathion and chlorfenvinphos determinations in the emulsifiable concentrate are 2.4 and 1.0%, respectively. Recoveries of the former from a fouled dip ranged from 97 to 103% and recoveries of the latter ranged from 92 to 97%.

Micro–Flow Imaging and Resonant Mass Measurement (Archimedes) – Complementary Methods to Quantitatively Differentiate Protein Particles and Silicone Oil Droplets

2013 ◽  
Vol 102 (7) ◽  
pp. 2152-2165 ◽  
Author(s):  
Daniel Weinbuch ◽  
Sarah Zölls ◽  
Michael Wiggenhorn ◽  
Wolfgang Friess ◽  
Gerhard Winter ◽  
...  
Keyword(s):  
Silicone Oil ◽  
Mass Measurement ◽  
Flow Imaging ◽  
Oil Droplets ◽  
Complementary Methods ◽  
Micro Flow ◽  
Protein Particles

An evaluation of kits for measuring thyrotropin in newborns.

1984 ◽  
Vol 30 (11) ◽  
pp. 1854-1856 ◽  
Author(s):  
E A Pierson ◽  
B L Therrell ◽  
S F Franz ◽  
A J Rudenstein ◽  
P E Dziuk
Keyword(s):  
United States ◽  
Filter Paper ◽  
Whole Blood ◽  
The United States ◽  
Medical Systems ◽  
Becton Dickinson ◽  
Screening Programs ◽  
Coefficients Of Variation ◽  
Blood Specimens

Abstract Several commercial radioimmunoassay kits are now marketed specifically for determination of thyrotropin (TSH) from whole-blood specimens collected on filter paper in neonatal screening programs. We have compared five kits in use in such screening programs in the United States. The reagent kits from Becton Dickinson, Neometrics, and Nuclear Medical Systems gave similar satisfactory results. That from Pharmacia was somewhat more difficult to use and gave greater coefficients of variation. Diagnostic Products' kit appeared to perform satisfactorily, but the analytical values obtained were significantly low, which may suggest erroneous calibrator values within the kit.

89 Comparative Quantification of Plasma Progesterone Through Radioimmumoassay and Enzyme-Linked Fluorescent Assay Techniques in Cattle

2018 ◽  
Vol 30 (1) ◽  
pp. 184
Author(s):  
J. M. R. Pérsico ◽  
C. Bianchi ◽  
C. Tapia ◽  
S. Raggio ◽  
I. A. Marchetti
Keyword(s):  
Predictive Value ◽  
Prostaglandin F2α ◽  
Standard Technique ◽  
Diagnostic Assay ◽  
Becton Dickinson ◽  
Bovine Reproduction ◽  
Coefficients Of Variation ◽  
In Vitro Diagnostic ◽  
Group B

Progesterone (P4) is an important component of oestrous cyclicity and is critical to fertility. A concentration >1 ng mL−1 reflects the function of the corpus luteum (CL) and is considered indicative of a cyclic cow. Recent publications have shown that P4 at the onset of synchronization programs is critical to pregnancy outcomes in primiparous cows (Stevenson et al. 2015 J. Anim. Sci. 93, 2111-2123) and cows with P4 <5 ng mL−1 on Day 14 could predict pregnancy loss (Kenyon et al. 2013 Anim. Reprod. Sci. 136, 223-230). Currently, the gold standard technique to quantify P4 is radioimmunoassay (RIA). However, new techniques are emerging. The objective of this study was to evaluate a new commercial in vitro diagnostic assay to quantify P4 based on enzyme immunoassay by competition with detection of final fluorescence (enzyme-linked fluorescence assay, ELFA). A total of 30 cows were synchronized on Day 0 with an intravaginal device (IVD) containing 500 mg of P4 (Cronipres, Biogénesis Bagó, Argentina). On Day 7 and Day 8 all cows received 150 mg of prostaglandin F2α (Enzaprost, Biogénesis Bagó). All IVD were removed on Day 8. A total of 95 blood samples were taken at Days 0, 9, 9.5, and 10 using BD Vacutainer (Becton-Dickinson, Franklin Lakes, NJ, USA) with sodium heparin by jugular venipuncture and centrifuged at 3000 × g for 30 min for plasma separation, which was frozen at –20°C until analysis. Samples were measured in duplicate by IM1188-Progesterone-RIA (Beckman Coulter, Brea, CA, USA) and VIDAS-PRG-ELFA (Biomerieux, Marcy l’Étoile, France). Concentrations of P4 obtained by RIA were classified in 2 groups: (A) P4 <1 ng mL−1, and (B) P4 ≥1 ng mL−1 and matched with P4 concentrations obtained by ELFA. Kappa (κ) test was used to determine agreement between both techniques, intra-assay coefficients of variation was determined for RIA and ELFA; and sensitivity (SE), specificity (SP), positive predictive value (PPV), and negative predictive value (NPV) were determined for ELFA. There was very good agreement between the RIA and ELFA techniques, κ = 0.95, to determine P4 concentrations for group A (62 and 62 samples, respectively) and group B (33 and 33 samples, respectively). The intra-assay coefficients of variation were 5% (RIA) and 2.9% (ELFA). Values for SE = 0.97, SP = 0.98, PPV = 0.97, and NPV = 0.98 were obtained for ELFA. We were able to quantify P4 in bovine plasma in all samples using the ELFA technique with similar reliability to the RIA technique. Only 2 samples (2.1%) differed in their concentrations and clinical interpretation. There was a slight discrepancy between the results found for both techniques with an excellent SE and SP in ELFA compared with RIA. Based on the analytical results, we believe that this in vitro diagnostic assay developed for use with an autoanalyzer could be useful for routine bovine reproduction programs. Further studies should be carried out to strengthen these conclusions.

Berlin prehospital or usual delivery of acute stroke care – Study protocol

2017 ◽  
Vol 12 (6) ◽  
pp. 653-658 ◽  
Author(s):  
Martin Ebinger ◽  
Peter Harmel ◽  
Christian H Nolte ◽  
Ulrike Grittner ◽  
Bob Siegerink ◽  
...  
Keyword(s):  
Sample Size ◽  
Functional Outcome ◽  
Primary Outcome ◽  
Stroke Care ◽  
Control Group ◽  
Modified Rankin Scale ◽  
Acute Stroke Care ◽  
Significance Level ◽  
Regular Care ◽  
Prehospital Triage

Rationale Prehospital stroke care in specialized ambulances increases thrombolysis rates, reduces alarm-to-treatment times, and improves the prehospital triage. Preliminary analyses suggest cost-effectiveness. However, scientific proof of better functional outcome compared to usual care is still lacking. Aim To prove better functional outcomes after deployment of the Stroke Emergency Mobile compared to regular ambulances. Sample size estimates A sample size of 686 patients will be required in each arm (Stroke Emergency Mobile group vs. regular care) to detect a difference regarding the primary outcome with 80% power at a two-sided significance level of 0.05. Methods and design This is a pragmatic, prospective study with blinded outcome assessment. Primary outcome will be functional status as defined by modified Rankin Scale score three months after the incident event. We will include cerebral ischemia patients within a predefined catchment area in Berlin, Germany. The study population consists of patients who might be candidates for acute recanalizing treatments, with onset-to-alarm time ≤4 h, symptoms not resolved at time of ambulance arrival, and able to walk without assistance prior to the qualifying incident. About 45% of Stroke Emergency Mobile dispatches are expected to be handled by regular ambulances, since Stroke Emergency Mobile will be already in operation creating the control group. Primary outcome Functional outcome after three months measured by the modified Rankin Scale over the entire range. Discussion The results will inform decision makers on the effectiveness of Stroke Emergency Mobile.

scholarly journals Particle-Size Distribution of Pulverized Snow

1959 ◽  
Vol 3 (26) ◽  
pp. 512-521
Author(s):  
H. H. G. Jellinek ◽  
Warren A. Schlueter
Keyword(s):  
Particle Size ◽  
Particle Size Distribution ◽  
Size Distribution ◽  
Silicone Oil ◽  
Glass Slide ◽  
Microscopic Method ◽  
Cross Sectional ◽  
Micrometer Eyepiece ◽  
Number Of Particles

Abstract A microscopic method for the determination of particle-size distribution of pulverized snow was worked out. The method gives satisfactory distribution curves, presenting the number of particles as a function of their cross-sectional “areas”. The measurements were made by means of a filar micrometer eyepiece, the snow particles being placed on a ruled glass slide, which was submerged in silicone oil to prevent evaporation. The time for the determination of a distribution can be appreciably shortened by estimating the size of the particles instead of measuring them, though the accuracy is not so high in this case.

Release of silicone oil and the off-label use of syringes in ophthalmology

2019 ◽  
Vol 104 (2) ◽  
pp. 291-296 ◽  
Author(s):  
Gustavo Barreto Melo ◽  
Geoffrey Guy Emerson ◽  
Celso Souza Dias Jr ◽  
Fábio Barreto Morais ◽  
Acacio de Souza Lima Filho ◽  
...  
Keyword(s):  
Intravitreal Injection ◽  
Silicone Oil ◽  
Fluid Temperature ◽  
Molecular Compound ◽  
Off Label Use ◽  
Becton Dickinson ◽  
Off Label ◽  
Different Temperatures ◽  
The Usa ◽  
The Impact

Background/aimsTo assess silicone oil (SO) release by different brands of syringes used for intravitreal injection under different handling conditions.MethodsEight syringes were analysed: from the USA, Terumo 0.5 mL, Becton-Dickinson (BD) Tuberculin 1 mL, BD Luer-lok 1 mL, BD Ultra-Fine 0.3 mL and Exel Insulin 0.3 mL; from Germany, Braun Omnifix-F 1 mL and Braun Injekt-F 1 mL and from Spain, BD Plastipak 1 mL. The impact of air, priming the plunger, agitation by flicking and fluid temperature on SO release were assessed by light microscopy. Fourier transform infrared spectroscopy (FTIR) was performed to identify the molecular compound in each syringe.ResultsFive hundred and sixty syringes were analysed. Terumo 0.5 mL and BD Ultra-Fine 0.3 mL released more SO than all others. BD Luer-lok 1 mL, BD Plastipak and Braun Omnifix-F 1 mL released little SO; BD Tuberculin 1 mL, Exel 0.3 mL and Braun Injekt-F 1 mL released the least SO. Priming the syringe and different temperatures did not significantly affect SO release. Agitation by flicking caused a significantly higher proportion of samples to have SO droplets and an increased number of oil droplets. Air had an additive effect on the release of oil in the agitation groups. FTIR identified polysiloxane in all syringes but Injekt-F.ConclusionSyringes commonly used for intravitreal injections frequently release SO droplets, especially when agitated by flicking. To avoid unnecessary ocular risks, syringes should not be agitated before intravitreal injection. It is desirable that syringes be manufactured specifically for ophthalmic use.

scholarly journals Development and Verification of an Adjustment Factor for Determining the Axial Length Using Optical Biometry in Silicone Oil-Filled Eyes

Diagnostics ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 163
Author(s):  
Gerd U. Auffarth ◽  
Tadas Naujokaitis ◽  
Louise Blöck ◽  
Anna Daghbashyan ◽  
Jan Meis ◽  
...  
Keyword(s):  
Axial Length ◽  
Silicone Oil ◽  
Systematic Difference ◽  
Partial Coherence ◽  
Prospective Clinical Study ◽  
Partial Coherence Interferometry ◽  
Coefficients Of Variation ◽  
Strong Linear Correlation ◽  
The Mean ◽  
Iolmaster 700

The aim of this prospective clinical study was to establish and verify an adaptation for axial length (AL) measurement in silicone oil (SO)-filled pseudophakic eyes with a Scheimpflug and partial coherence interferometry (PCI)-based biometer. The AL was measured with a Pentacam AXL (OCULUS Optikgeräte GmbH, Wetzler, Germany) and IOLMaster 700 (Carl Zeiss Meditec, Jena, Germany). The coefficients of variation (CoV) and the mean systematic difference (95% confidence interval (CI)) between the devices were calculated. After implementing a setting for measuring AL in tamponaded eyes with a Pentacam based on data of 29 eyes, another 12 eyes were examined for verification. The mean AL obtained with the Pentacam was 25.53 ± 1.94 mm (range: 21.70 to 30.76 mm), and with IOLMaster, 24.73 ± 1.97 mm (ranged 20.84 to 29.92 mm), resulting in a mean offset of 0.80 ± 0.08 mm (95% CI: 0.77, 0.83 mm), p < 0.001. The AL values of both devices showed a strong linear correlation (r = 0.999). Verification data confirmed good agreement, with a statistically and clinically non-significant mean difference of 0.02 ± 0.04 (95% CI: −0.01, 0.05) mm, p = 0.134. We implemented a specific adaptation for obtaining reliable AL values in SO-filled eyes with the Pentacam AXL.

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