scholarly journals Investigation of antioxidant and anticancer activities of unsaturated oligo-galacturonic acids produced by pectinase of Streptomyces hydrogenans YAM1

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Afrouzossadat Hosseini Abari ◽  
Hamed Amini Rourani ◽  
Seyed Mahdi Ghasemi ◽  
Hyun Kim ◽  
Yun-Gon Kim
Keyword(s):  
Radical Scavenging Activity ◽  
Flow Cytometric Analysis ◽  
Radical Scavenging ◽  
Anticancer Activities ◽  
Pectin Degradation ◽  
New Approach ◽  
Flight Mass Spectrometry ◽  
Highly Active ◽  
Carbohydrate Polymer ◽  
Pectinase Activity

AbstractPectin, a diverse carbohydrate polymer in plants consists of a core of α-1,4-linked D-galacturonic acid units, includes a vast portion of fruit and agricultural wastes. Using the wastes to produce beneficial compounds is a new approach to control the negative environmental impacts of the accumulated wastes. In the present study, we report a pectinase producing bacterium Streptomyces hydrogenans YAM1 and evaluate antioxidative and anticancer effects of the oligosaccharides obtained from pectin degradation. The production of oligosaccharides due to pectinase activity was detected by thin layer chromatography (TLC) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Our results revealed that S. hydrogenans YAM1 can degrade pectin to unsaturated pectic oligo-galacturonic acids (POS) with approximately 93% radical scavenging activity in 20 mg/mL which it is more than 50% of the same concentration of pectin. Flow cytometric analysis revealed that MCF-7 cells viability decreased more than 32 and 92% following treatment with 6 and 20 mg/mL POS after 24 h, respectively. It is suggested that pectin degradation by S. hydrogenans YAM1 is not only a new approach to produce highly active compounds from fruit wastes, but also is an effective method to remove fibrous pollutants from different environments.

scholarly journals Antioxidant and Anticancer Activities of Wampee (Clausena lansium(Lour.) Skeels) Peel

2009 ◽  
Vol 2009 ◽  
pp. 1-6 ◽  
Author(s):  
K. Nagendra Prasad ◽  
Jing Hao ◽  
Chun Yi ◽  
Dandan Zhang ◽  
Shengxiang Qiu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Liver Carcinoma ◽  
Scavenging Activity ◽  
Anticancer Activities

Antioxidant activities of wampee peel extracts using five different solvents (ethanol, hexane, ethyl acetate, butanol and water) were determined by using in-vitro antioxidant models including total antioxidant capability, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, reducing power, and superoxide scavenging activity. Ethyl acetate fraction (EAF) exhibited the highest antioxidant activity compared to other fractions, even higher than synthetic antioxidant butylated hydroxyl toluene (BHT). In addition, the EAF exhibited strong anticancer activities against human gastric carcinoma (SGC-7901), human hepatocellular liver carcinoma (HepG-2) and human lung adenocarcinoma (A-549) cancer cell lines, higher than cisplatin, a conventional anticancer drug. The total phenolic content of wampee fraction was positively correlated with the antioxidant activity. This is the first report on the antioxidant and anticancer activities of the wampee peel extract. Thus, wampee peel can be used potentially as a readily accessible source of natural antioxidants and a possible pharmaceutical supplement.

scholarly journals BIOACTIVE MARINE-DERIVED COMPOUNDS AS POTENTIAL ANTICANCER CANDIDATES

2018 ◽  
Vol 11 (11) ◽  
pp. 464 ◽  
Author(s):  
Mona S Ellithey ◽  
Hanaa H Ahmed
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Radical Scavenging Activity ◽  
Research Work ◽  
Radical Scavenging ◽  
Cancer Cell Lines ◽  
Anticancer Activities ◽  
Vitro Assay ◽  
Safety Margins

Objective: The objective of this research was to evaluate for the 1st time the anticancer activities of sarcophytol M (1), alismol (2), alismoxide (5), 7β-acetoxy-24-methylcholesta-5-24(28)-diene-3,19-diol (7), erythro-N-dodecanoyl-docosasphinga-(4E,8E)-dienine (8), and 24 methylcholesta- 5,24(28)-diene-3β,7β,19-triol (9). Compounds were isolated from the soft coral Lithophyton arboreum and tested in liver (HepG2), lung (A549), and breast (MDA) cancer cell line.Methods: Anticancer activities of the compounds were tested using (XTT) 2,3-bis-(2-methoxy-4-nitro- 5-sulfophenyl)-2H-tetrazolium-5-carboxanilide, Na2) in vitro assay in order to estimate the cytotoxicity and to determine the IC50s. The free radical scavenging activity of the compounds were measured by 1,1-diphenyl-2-picryl-hydrazil (DPPH•). All compounds were screened at 100 μg/ml while the most potent active compounds were assayed at lower concentrations.Results: Compounds (7) and (9) showed a strong cytotoxic effect with IC50 of 6.07, 8.5 μg/ml in HepG2, 6.3, 5.5 μg/ml in MDA cells, and 5.2, 9.3 μg/ml in A549 cancer cell lines, respectively. In addition, moderate cytotoxicity was shown by compound (2) (IC50 16.5, 15, and 13 μg/mL) in HepG2, MDA, and A549 cancer cell lines, respectively.Conclusion: The results obtained in this research work indicated a promising potential cytotoxicity of compounds (7) and (9) compared to its safety margins in Vero cells, and the expected cytostatic effect of compound (2) can be used in drug cocktails for the treatment of the major cancer types’ lung, breast, and liver cancer. 

scholarly journals Characterization of the Phytochemical Constituents of Taif Rose and Its Antioxidant and Anticancer Activities

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
El-Sayed S. Abdel-Hameed ◽  
Salih A. Bazaid ◽  
Mahmood S. Salman
Keyword(s):  
Anticancer Activity ◽  
Total Phenolics ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Anticancer Activities ◽  
High Positive Correlation ◽  
Methanol Extracts ◽  
Natural Agents

Ward Taifi (Taif rose) is considered one of the most important economic products of Taif, Saudi Arabia. In this study both fresh and dry Taif rose were biologically and phytochemically investigated. The 80% methanol extracts andn-butanol fractions of dry and fresh Taif rose had high radical scavenging activity toward artificial 1,1-diphenyl picrylhydrazyl (DPPH)•radical with SC50values range 5.86−12.24 µg/ml whereas the aqueous fractions showed weak activity. All samples hadin vitroanticancer activity toward HepG2 with IC50< 20 µg/ml which fall within the criteria of the American Cancer Institute. High positive correlation appeared between the antioxidant activity and total phenolics whereas there is no correlation between total phenolics and anticancer activity. The LC-ESI(− ve)-MS analysis of all extracts indicate the presence of phenolic compounds belonging to hydrolysable tannins and flavonol glycosides. In conclusion, the presence of this is considered to be the first phytochemical report that identifies the major compounds in dry and fresh roses using HPLC-ESI-MS. The methanol extracts and itsn-butanol and aqueous fractions for both fresh and dry Taif rose could be used as preventive and therapeutic effective natural agents for diseases in which free radicals involved after morein vitroandin vivostudies.

Pyrrolo[1,2-a]azepines Coupled with Benzothiazole and Fluorinated Aryl Thiourea Scaffolds as Promising Antioxidant and Anticancer Agents

2019 ◽  
Vol 19 (15) ◽  
pp. 1855-1862
Author(s):  
Rahul V. Patel ◽  
Bhupendra M. Mistry ◽  
Riyaz Syed ◽  
Nikhil M. Parekh ◽  
Han-Seung Shin
Keyword(s):  
Anticancer Agents ◽  
Mdck Cells ◽  
Radical Scavenging Activity ◽  
Small Cell Lung Carcinoma ◽  
Radical Scavenging ◽  
Colon Adenocarcinoma ◽  
Cytotoxic Agents ◽  
Health Concern ◽  
Anticancer Activities ◽  
Cell Lung Carcinoma

Background: Cancer remains a major health concern throughout history and is responsible for huge numbers of deaths globally. The sensitivity of cancer cells to anticancer drugs is a crucial factor for developing effective treatments. Methods: Pyrrolo[1,2-a]azepines coupled with benzothiazole and fluorinated aryl thiourea scaffolds have been synthesized, and their potential as cytotoxic agents was investigated against different cancer cell lines such as human ovarian cancer (SK-OV-3), cervical cancer (HeLa), colon adenocarcinoma (HT-29) and non-small-cell lung carcinoma (A549). Cytotoxicity of new compounds was confirmed using SRB assay against non-cancer MDCK cell line. In addition, free radical scavenging activity of new pyrrolo[1,2-a]azepines was examined by adopting DPPH and ABTS assays. Results: The results concluded that the presence and position of fluorine atom(s) on the thiourea unit played a significant role in order to gain anticipated efficacies. Results of the cytotoxic assay against non-cancer MDCK cells showed that these new derivatives are safe to study further. New structures were confirmed using spectral and elemental analyses. Conclusion: Pyrrolo[1,2-a]azepines endowed with a benzothiazole entity and fluorinated aryl thiourea substituents were derived aiming to furnish remarkable antioxidant and anticancer activities. New molecules generated showed interesting biological result correlated with the structure and substituent of the final derivatives. Specifically, numbers and position of fluorine atoms on the thiourea unit influenced the biological profile of the mentioned compounds.

scholarly journals Comprehensive Investigation of the Effects of Brewing Conditions in Sample Preparation of Green Tea Infusions

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1735 ◽  
Author(s):  
Yan Jin ◽  
Jing Zhao ◽  
Eun Mi Kim ◽  
Ki Hyun Kim ◽  
Seulgi Kang ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Green Tea ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Careful Consideration ◽  
Biological Investigation ◽  
Preparation Conditions ◽  
Flight Mass Spectrometry ◽  
Tea Infusions

Chemical and biological investigation of green tea has been generally performed while using different infusions that are prepared without consideration of the effects of sample preparation conditions. In this study, for the first time, the effects of green tea brewing conditions on the antioxidant activity and chemical profiles of metabolome and catechin compounds were examined at 60 °C and 95 °C for a period of 5–300 min. The antioxidant capacities of the tea infusions, which were assessed as per 2,2-diphenyl-1-picryl-hydrazyl hydrate (DPPH) radical scavenging activity, depended more on temperature than time. Metabolomics study that was based on ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UHPLC-QTOF/MS) revealed that the metabolic profiles, including 33 differential metabolites, were significantly changed by temperature and time, with the effects of time being more evident at 95 °C starting after 30 min. Infusions that were brewed at 95 °C for greater than 30 min yielded distinct profiles in the hierarchical clustering analysis. The quantification of eight catechins by UHPLC-QqQ/MS showed that the total catechin level peaked at 95 °C brewing at 10 min, after which the levels of four epi-forms of catechins decreased and those of four non-epi-forms increased, implying the epimerization of catechins over time. These results suggest that the brewing conditions for sample preparation of green tea should be put into careful consideration in studies where green tea extracts are applied as aqueous infusions.

scholarly journals Antioxidant Activities of Artemisia Absinthium and Citrus Paradisi Extracts Repress Viability of Aggressive Liver Cancer Cell Line

2021 ◽  
Author(s):  
Muhammad Ali ◽  
Rida Iqbal ◽  
Muhammad Safdar ◽  
Sehrish Murtaza ◽  
Ghulam Mustafa ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cell Line ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Citrus Paradisi ◽  
Anticancer Activities ◽  
Artemisia Absinthium ◽  
Cancerous Cell ◽  
Dpph Radical Scavenging

Abstract Numerous reports show that herbal medicines can be utilized in the treatment of different liver disorders. In this study, antioxidant, antibacterial, and anticancer activities of individual as well as combined ethanolic extracts of Artemisia absinthium leaves and Citrus paradisi peels were investigated. Values of total phenolic contents (TPC), total flavonoid contents (TFC), DPPH-radical scavenging activity, and ferric reducing antioxidant power (FRAP) were measured to explore the antioxidant capacity. To assess antibacterial activity, four bacterial strains were used. For evaluation of anticancer activity Huh-7 (liver cancer) and Vero (non-cancerous) cell lines were utilized. FRAP activity of combined plants extract was higher as compared to their individual effect; the trend did not hold in case of DPPH-radical scavenging activity. Disk diffusion method revealed that only combined extract of plants express antibacterial activity against one strain (E. coli). MTT results indicated that both plants have cytotoxic effect on Huh-7 cell line and did not show any effect on Vero cell line. Our data showed a strong negative correlation between the amount of TPC, TFC, & DPPH radicals-scavenging activity and cell viability of cancerous cell line. Whereas, no effect was shown on non-cancerous cell line. Hence, these plants can be used in treatment of liver cancer because of their antioxidant, antibacterial, and anticancer activities.

scholarly journals Comparison of Radical Scavenging Activity, Cytotoxic Effects and Apoptosis Induction in Human Melanoma Cells by Taiwanese Propolis from Different Sources

2004 ◽  
Vol 1 (2) ◽  
pp. 175-185 ◽  
Author(s):  
Chia-Nan Chen ◽  
Meng-Shih Weng ◽  
Chia-Li Wu ◽  
Jen-Kun Lin
Keyword(s):  
Radical Scavenging Activity ◽  
Flow Cytometric Analysis ◽  
Radical Scavenging ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Scavenging Activity ◽  
Human Melanoma Cells ◽  
Antioxidant Agents ◽  
Trigger Activity ◽  
High Level

Propolis is a sticky substance that is collected from plants by honeybees. We previously demonstrated that propolins A, B, C, D, E and F, isolated from Taiwanese propolis (TP), could effectively induce human melanoma cell apoptosis and were strong antioxidant agents. In this study, we evaluated TP for free radical scavenging activity by DPPH (1,2-diphenyl-2-picrylhydrazyl). The phenolic concentrations were quantified by the Folin–Ciocalteu method. The apoptosis trigger activity in human melanoma cells was evaluated. TP contained a higher level of phenolic compounds and showed strong capability to scavenge free radicals. Additionally, TP1g, TP3, TP4 and TP7 exhibited a cytotoxic effect on human melanoma cells, with an IC50 of 2.3, 2.0, 3.3 and 3.3 μg/ml, respectively. Flow cytometric analysis for DNA fragmentation indicated that TP1g, TP2, TP3 and TP7 could induce apoptosis in human melanoma cells and there is a marked loss of cells from the G2/M phase of the cell cycle. To address the mechanism of the apoptosis effect of TP, we evaluated its effects on induction of apoptosis-related proteins in human melanoma cells. The levels of procaspase-3 and PARP [poly(ADP-ribose) polymerase] were markedly decreased. Furthermore, propolins A, B, C, D, E and F in TP were determined using HPLC. The results indicate that TP is a rich source of these compounds. The findings suggest that TP induces apoptosis in human melanoma cells due to its high level of propolins.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN PADA EKSTRAK ETANOL DAUN JAMBU METE (Anacardium occidentale L.)

2018 ◽  
Vol 5 (1) ◽  
pp. 47
Author(s):  
Dina Soes Putri ◽  
Muti’ah Muti’ah ◽  
Yunita Arian Sani Anwar
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Active Fraction ◽  
Antioxidant Assay ◽  
Water Fraction ◽  
Highly Active ◽  
Ic50 Value ◽  
Dpph Method

Tujuan dari penelitian ini adalah mengetahui metabolit sekunder apa saja yang berpotensi sebagai antioksidan dari ekstrak etanol daun jambu mete dan mengetahui aktivitas antioksidan dari ekstrak etanol daun jambu mete. Pada penelitian ini telah dilakukan penapisan fitokimia dan uji aktivitas antioksidan pada ekstrak etanol dan fraksi-fraksi ekstrak etanol daun jambu mete muda yang berasal dari daerah Kayangan, Lombok Utara. Berdasarkan uji fitokimia diketahui bahwa ekstrak etanol dari daun jambu mete muda mengandung metabolit sekunder jenis polifenol, tanin, flavonoid, dan steroid. Dari hasil uji aktivitas antioksidan dengan metode DPPH disimpulkan bahwa ekstrak etanol dan fraksi-fraksinya bersifat aktif sebagai antioksidan terhadap radikal bebas DPPH. Dimana, fraksi etanol-air merupakan fraksi yang paling aktif dengan nilai IC­50 sebesar 0,26 ppm, sedangkan fraksi yang paling kurang aktif adalah fraksi n-heksan dengan IC50 sebesar 10,72 ppm. The aims of this research were to find out which secondary metabolite that potential as antioxidant from ethanol extract of cashew leaf and to find out the radical scavenging activity from ethanol extract of cashew leaf. The sample of this research was the young leaf of cashew taken from Kayangan, North Lombok. Phytochemical screening and antioxidant assay have been conducted to ethanol extract and its fractions. The result of phytochemical screening shown that etanol extract of young cashew leaf contain of polyphenol, tannin, flavonoid, and steroid. Based on antioxidant assay using DPPH method, it can be concluded that ethanol extract and its fractions were highly active as scaveging agent compared to synthetic antioxidant BHA. Where, ethanol-water fraction is the most active fraction with IC50 value of 0,26 ppm, while the least active fraction is n-hexane fraction with IC50 value of 10.72 ppm.

scholarly journals Evaluation of the Effects of Some Brazilian Medicinal Plants on the Production of TNF-αand CCL2 by THP-1 Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Grasielle S. Gusman ◽  
Priscilla R. V. Campana ◽  
Luciano C. Castro ◽  
Rachel O. Castilho ◽  
Mauro M. Teixeira ◽  
...  
Keyword(s):  
Inflammatory Diseases ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Leukemia Cell ◽  
Leukemia Cell Line ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Monocytic Leukemia ◽  
Concentration Dependent Manner ◽  
Highly Active

Several plant species are traditionally used in Brazil to treat various inflammatory diseases. Tumor necrosis factor- (TNF-)αand chemokine (C-C motif) ligand 2 (CCL2) are key inflammatory mediators in diseases like rheumatoid arthritis and atherosclerosis, respectively; nevertheless, only a few extracts have been assayed against these targets. We herein report the effect of 19 plant extracts on TNF-αand CCL2 release by lipopolysaccharide- (LPS-) stimulated THP-1 cells, a human monocytic leukemia cell line, along with their radical scavenging activity on DPPH. The extracts ofCaryocar brasiliense,Casearia sylvestris,Coccoloba cereifera, andTerminalia glabrescensinhibited TNF-αproduction in a concentration-dependent manner. Fractionation of these extracts potentiated the anti-TNF-αeffect, which was shown to concentrate in polar fractions, mainly composed by polyphenols. Significant CCL2 inhibition was elicited byLippia sidoidesandTerminalia glabrescensextracts, whose fractionation resulted in highly active low polar fractions. All assayed extracts showed strong radical scavenging activity, but antioxidant activity did not correlate with inhibition of TNF-αor CCL2 production. Our results allowed identifying extracts with selective capacity to block cytokine production; therefore, further purification of these extracts may yield molecules that could be useful in the treatment of chronic inflammatory diseases.

scholarly journals Synthesis of 4,4′-(arylmethylene)bis(3-methyl-1-phenyl-1H-pyrazol-5-ols) and evaluation of their antioxidant and anticancer activities

BMC Chemistry ◽  
2021 ◽  
Vol 15 (1) ◽  
Author(s):  
José Eduardo Cadena-Cruz ◽  
Luis M. Guamán-Ortiz ◽  
Juan Carlos Romero-Benavides ◽  
Natalia Bailon-Moscoso ◽  
Kevin E. Murillo-Sotomayor ◽  
...  
Keyword(s):  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Carcinoma Cells ◽  
Structural Motif ◽  
Scavenging Activity ◽  
Anticancer Activities ◽  
Different Types ◽  
Simple Filtration

Abstract Background Pyrazoles have attracted particular attention due to the diverse biological activities associated with this heterocyclic system, and some have been shown to be cytotoxic to several human cell lines. Several drugs currently on the market have this heterocycle as the key structural motif, and some have been approved for the treatment of different types of cancer. Results 4,4ʹ-(Arylmethylene)bis(1H-pyrazol-5-ols) derivatives 3a–q were synthetized by a three components reaction of 3-methyl-1-phenyl-5-pyrazolone (1) with various benzaldehydes 2 catalyzed by sodium acetate at room temperature. The structures of all synthesized compounds were characterized by physicochemical properties and spectral means (IR and NMR) and were evaluated for their radical scavenging activity by DPPH assay and tested in vitro on colorectal RKO carcinoma cells in order to determine their cytotoxic properties. All 4,4ʹ-(arylmethylene)bis(1H-pyrazol-5-ols) derivatives 3a–q were synthetized in high to excellent yield, and pure products were isolated by simple filtration. All compounds have good radical scavenging activity, and half of them are more active than ascorbic acid used as standard. Conclusion Several derivatives proved to be cytotoxic in the RKO cell line. In particular, compound 3i proved to be a very potent scavenger with an IC50 of 6.2 ± 0.6 µM and exhibited an IC50 of 9.9 ± 1.1 μM against RKO cell. Autophagy proteins were activated as a survival mechanism, whereas the predominant pathway of death was p53-mediated apoptosis.

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