scholarly journals Newly discovered Synechococcus sp. PCC 11901 is a robust cyanobacterial strain for high biomass production

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Artur Włodarczyk ◽  
Tiago Toscano Selão ◽  
Birgitta Norling ◽  
Peter J. Nixon
Keyword(s):  
Production Systems ◽  
Cyanobacterial Strain ◽  
Green Biotechnology ◽  
Dry Cell Weight ◽  
Wide Range ◽  
Synechococcus Sp ◽  
Dry Cell ◽  
Short Doubling Time ◽  
Heterotrophic Production

AbstractCyanobacteria, which use solar energy to convert carbon dioxide into biomass, are potential solar biorefineries for the sustainable production of chemicals and biofuels. However, yields obtained with current strains are still uncompetitive compared to existing heterotrophic production systems. Here we report the discovery and characterization of a new cyanobacterial strain, Synechococcus sp. PCC 11901, with promising features for green biotechnology. It is naturally transformable, has a short doubling time of ≈2 hours, grows at high light intensities and in a wide range of salinities and accumulates up to ≈33 g dry cell weight per litre when cultured in a shake-flask system using a modified growth medium − 1.7 to 3 times more than other strains tested under similar conditions. As a proof of principle, PCC 11901 engineered to produce free fatty acids yielded over 6 mM (1.5 g L−1), an amount comparable to that achieved by similarly engineered heterotrophic organisms.

scholarly journals Unprecedented biomass and fatty acid production by the newly discovered cyanobacteriumSynechococcussp. PCC 11901

2019 ◽  
Author(s):  
Artur Włodarczyk ◽  
Tiago Toscano Selão ◽  
Birgitta Norling ◽  
Peter J. Nixon
Keyword(s):  
Production Systems ◽  
Cyanobacterial Strain ◽  
Fatty Acid Production ◽  
Green Biotechnology ◽  
Dry Cell Weight ◽  
Wide Range ◽  
Dry Cell ◽  
Short Doubling Time ◽  
Heterotrophic Production

AbstractCyanobacteria, which use solar energy to convert carbon dioxide into biomass, are potential solar biorefineries for the sustainable production of chemicals and biofuels. However, yields obtained with current strains are still uncompetitive compared to existing heterotrophic production systems. Here we report the discovery and characterization of a new cyanobacterial strain,Synechococcussp. PCC 11901, with potential for green biotechnology. It is transformable, has a short doubling time of ≈2 hours, grows at high light intensities and a wide range of salinities and accumulates up to 18.3 g dry cell weight/L of biomass – 2-3 times more than previously described for cyanobacteria - when grown in a modified medium containing elevated nitrate, phosphate and iron. As a proof of principle, PCC 11901 engineered to produce free fatty acids did so at unprecedented levels for cyanobacteria, with final yields reaching over 6 mM (1.5 g/L), comparable to those achieved by heterotrophic organisms.

scholarly journals Effect of Carbon Sources, Nitrogen Sources and Prebiotics on Growth of Saccharomyces Boulardii

2019 ◽  
Vol 23 (2) ◽  
pp. 101-108
Author(s):  
Xin Yang ◽  
Guowei Shu ◽  
Zhangteng Lei ◽  
Guanli Du ◽  
Zemin Liu ◽  
...  
Keyword(s):  
Yeast Extract ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Gastrointestinal Diseases ◽  
Potassium Nitrate ◽  
Soluble Starch ◽  
Saccharomyces Boulardii ◽  
Dry Cell Weight ◽  
Wide Range ◽  
Dry Cell

Abstract Saccharomyces boulardii (S. boulardii) has been used as a probiotic for the prevention or treatment of various human gastrointestinal diseases for many years. Thus, S. boulardii has a wide range of application prospects in medicine and food industry. The experiments were investigated with effecting of carbon sources (galactose, sucrose, fructose, maltose, lactose, glucose, and soluble starch), nitrogen sources (tryptone, casein, yeast extract, peptone, soy peptone, beef extract and malted milk; ammonium sulfate, urea, diammonium hydrogen citrate, triammonium citrate, ammonium nitrate, ammonium chloride and potassium nitrate), prebiotics (xylo-oligosaccharide, isomaltooligosaccharide, fructo-oligosaccharide, galacto-oligosaccharide, stachyose, raffinose and inulin) on the number of viable cells and dry cell weight of S. boulardii. The optimum concentration of 5 % glucose or sucrose, 2 % peptone or yeast extract, 0.4 % urea, 0.2 % fructo-oligosaccharide and 0.6 % galacto-oligosaccharide for S. boulardii grew better in medium.

scholarly journals The Marine Sponge Petrosia ficiformis Harbors Different Cyanobacteria Strains with Potential Biotechnological Application

2020 ◽  
Vol 8 (9) ◽  
pp. 638
Author(s):  
Patrizia Pagliara ◽  
Amilcare Barca ◽  
Tiziano Verri ◽  
Carmela Caroppo
Keyword(s):  
Cultured Cells ◽  
Biological Activities ◽  
Cell Type ◽  
High Sequence Identity ◽  
Toxic Activity ◽  
Bioactive Properties ◽  
Wide Range ◽  
Synechococcus Sp ◽  
Morphological And Molecular Characterization

Marine cyanobacteria are a source of bioactive natural compounds, with a wide range of biotechnological applications. However, information on sponge-associated cyanobacteria are relatively scarce to date. In this paper, we carried out the morphological and molecular characterization of eight cyanobacterial strains, previously isolated from the Mediterranean sponge Petrosia ficiformis, and evaluated their biological activities on epithelial- and neuron-like cultured cells of human and murine origin. The new analysis allowed maintaining the assignment of three strains (Cyanobium sp., Leptolyngbya ectocarpi, and Synechococcus sp.), while two strains previously identified as Synechococcus sp. and Leptolyngbya sp. were assigned to Pseudanabaena spp. One strain, i.e., ITAC104, and the ITAC101 strain corresponding to Halomicronema metazoicum, shared extremely high sequence identity, practically representing two clones of the same species. Finally, for only one strain, i.e., ITAC105, assignment to a specific genus was not possible. Concerning bioactivity analyses, incubation of cyanobacterial aqueous cell supernatants induced variable responses in cultured cells, depending on cell type, with some of them showing toxic activity on human epithelial-like cells and no toxic effects on human and rat neuron-like cells. Future investigations will allow to better define the bioactive properties of these cyanobacteria strains and to understand if they can be useful for (a) therapeutic purpose(s).

scholarly journals Fed-batch production and characterization of polyhydroxybutyrate by Bacillus megaterium LVN01 from residual glycerol

DYNA ◽  
2020 ◽  
Vol 87 (214) ◽  
pp. 111-120
Author(s):  
Javier Ricardo Gómez Cardozo ◽  
Rodrigo Velasco Bucheli ◽  
Nathalia Marín Pareja ◽  
Orlando Simón Ruíz Villadiego ◽  
Guillermo Antonio Correa Londoño ◽  
...  
Keyword(s):  
Bacillus Megaterium ◽  
Operating Conditions ◽  
Fed Batch ◽  
Fermentation Time ◽  
Batch Cultures ◽  
Batch Systems ◽  
Dry Cell Weight ◽  
Phb Production ◽  
Dry Cell

The operating conditions of polyhydroxybutyrate (PHB) production processes are among the factors that most influence yields. In this study, we evaluated PHB production synthesized by Bacillus megaterium LVN01. Batch and fed-batch cultures were used to produce PHB from residual glycerol. For this, dry cell weight (DCW) and PHB productivity were analyzed at a preliminary stage by central composite design using batch systems under different temperature, C/N ratio, and fermentation time conditions. The maximum PHB productivity occurred at 30.8 °C, 44.9 mol mol-1, and 39.9 h. The same conditions were tested for studies in fed-batch culture. Fed-batch experiments were comparable to each other, where the DCW was around 1.9 g L-1, with PHB productivities of 29.5 mg L-1 h-1 and 35.6 mg L-1 h-1 for bioreactors of 5 L and 14 L, respectively. The PHB was characterized by NMR, FTIR, DSC, TGA, and DTG analys

scholarly journals Isolation and Characterization of a Yellow Xanthophyll Pigment-Producing Marine Bacterium, Erythrobacter sp. SDW2 Strain, in Coastal Seawater

Marine Drugs ◽  
2022 ◽  
Vol 20 (1) ◽  
pp. 73
Author(s):  
Sun Wook Jeong ◽  
Jung Eun Yang ◽  
Yong Jun Choi
Keyword(s):  
Bioactive Compounds ◽  
Marine Bacterium ◽  
Industrial Applications ◽  
Yellow Pigment ◽  
Coastal Seawater ◽  
Isolation And Characterization ◽  
Dry Cell Weight ◽  
Dry Cell ◽  
Xanthophyll Pigment

Xanthophylls, a yellow pigment belonging to the carotenoid family, have attracted much attention for industrial applications due to their versatile nature. We report the isolation of a homo xanthophyll pigment-producing marine bacterium, identified as the Erythrobacter sp. SDW2 strain, from coastal seawater. The isolated Erythrobacter sp. SDW2 strain can produce 263 ± 12.9 mg/L (89.7 ± 5.4 mg/g dry cell weight) of yellow xanthophyll pigment from 5 g/L of glucose. Moreover, the xanthophyll pigment produced by the SDW2 strain exhibits remarkable antioxidative activities, confirmed by the DPPH (73.4 ± 1.4%) and ABTS (84.9 ± 0.7%) assays. These results suggest that the yellow xanthophyll pigment-producing Erythrobacter sp. SDW2 strain could be a promising industrial microorganism for producing marine-derived bioactive compounds with potential for foods, cosmetics, and pharmaceuticals.

Quantification of autolysis inPenicillium chrysogenumby semiautomated image analysis

2001 ◽  
Vol 47 (4) ◽  
pp. 315-321 ◽  
Author(s):  
M McIntyre ◽  
J K Eade ◽  
P W Cox ◽  
C R Thomas ◽  
S White ◽  
...  
Keyword(s):  
Image Analysis ◽  
Previous Method ◽  
Analysis Method ◽  
Image Analysis Method ◽  
Active Biomass ◽  
Additional Information ◽  
Dry Cell Weight ◽  
Automated Measurements ◽  
Dry Cell

An image analysis method is described for the characterization of empty (autolyzed and inactive) regions within the mycelia of filamentous fungi. It extends a previous method that characterized only regions filled with cytoplasm or vacuoles (i.e., the active biomass). The method is semiautomatic, requiring some manual editing before automated measurements. When the method was used for samples from a batch fermentation of an industrial strain of Penicillium chrysogenum, the empty regions were observed to constitute up to 15% (by projected area) of the biomass during the growth phase. After nutrient exhaustion, however, the proportion of empty regions rose rapidly, eventually representing more than 50% of the biomass by the end of fermentation. The increase in the percentage of empty regions coincided with a decrease in biomass (as measured by dry cell weight) and a fall in penicillin titre. Further morphological analysis revealed that fragmentation of mycelia, particularly clumps, coincided with increases in the levels of empty regions. This new image analysis method gave additional information on hyphal differentiation and a measure of autolysis. It was also a useful indicator of the processes leading to autolysis.Key words: autolysis, penicillin, differentiation, double grey.

Density and morphological characterization of bacteria in integrated and exclusive production systems

2020 ◽  
Vol 13 (11) ◽  
pp. 1
Author(s):  
A. R. B. Zanco ◽  
A. Ferreira ◽  
G. C. M. Berber ◽  
E. N. Gonzaga ◽  
D. C. C. Sabino
Keyword(s):  
Bacterial Community ◽  
Rainy Season ◽  
Production Systems ◽  
Cropping System ◽  
Morphological Characterization ◽  
Native Forest ◽  
Exclusive Production ◽  
Colony Forming Units

The different integrated production systems can directly interfere with its bacterial community. The present study aimed to assess density, bacterial diversity and the influence of dry and rainy season in different integrated and an exclusive production system. The fallow and a native forest area was assessed to. Samples were collected in 2012 March and September. The isolation were carried out into Petri dishes containing DYGS medium. The number of colony forming units (CFU) was counted after 48 hours and. The bacterial density ranged between 106 and 107 CFU g-1 soil. The crop system affected the dynamics of the bacterial community only in the rainy season. The rainy season showed greater density of total bacteria when compared to the dry period regardless of the cropping system. The dendrograms with 80 % similarity showed thirteen and fourteen groups in the rainy and dry seasons. Isolates with the capacity to solubilize phosphate in vitro were obtained from all areas in the two seasons, but this feature has been prevalent in bacteria isolated during the rainy season

Polymer Chemistry

2004 ◽  
Keyword(s):  
Conducting Polymers ◽  
Liquid Crystalline ◽  
Polymer Chemistry ◽  
Chain Growth ◽  
Crystalline Polymers ◽  
Wide Range ◽  
Cyclic Oligomers ◽  
Step Growth

Polymer Chemistry: A Practical Approach in Chemistry has been designed for both chemists working in and new to the area of polymer synthesis. It contains detailed instructions for preparation of a wide-range of polymers by a wide variety of different techniques, and describes how this synthetic methodology can be applied to the development of new materials. It includes details of well-established techniques, e.g. chain-growth or step-growth processes together with more up-to-date examples using methods such as atom-transfer radical polymerization. Less well-known procedures are also included, e.g. electrochemical synthesis of conducting polymers and the preparation of liquid crystalline elastomers with highly ordered structures. Other topics covered include general polymerization methodology, controlled/"living" polymerization methods, the formation of cyclic oligomers during step-growth polymerization, the synthesis of conducting polymers based on heterocyclic compounds, dendrimers, the preparation of imprinted polymers and liquid crystalline polymers. The main bulk of the text is preceded by an introductory chapter detailing some of the techniques available to the scientist for the characterization of polymers, both in terms of their chemical composition and in terms of their properties as materials. The book is intended not only for the specialist in polymer chemistry, but also for the organic chemist with little experience who requires a practical introduction to the field.

scholarly journals Organelle Genetics in Plants

2021 ◽  
Vol 22 (4) ◽  
pp. 2104
Author(s):  
Pedro Robles ◽  
Víctor Quesada
Keyword(s):  
Rna Editing ◽  
Posttranscriptional Regulation ◽  
Genome Engineering ◽  
Plant Mitochondria ◽  
Plastid Dna ◽  
Chloroplast Genomes ◽  
Wide Range ◽  
Organelle Genetics ◽  
Selection Of

Eleven published articles (4 reviews, 7 research papers) are collected in the Special Issue entitled “Organelle Genetics in Plants.” This selection of papers covers a wide range of topics related to chloroplasts and plant mitochondria research: (i) organellar gene expression (OGE) and, more specifically, chloroplast RNA editing in soybean, mitochondria RNA editing, and intron splicing in soybean during nodulation, as well as the study of the roles of transcriptional and posttranscriptional regulation of OGE in plant adaptation to environmental stress; (ii) analysis of the nuclear integrants of mitochondrial DNA (NUMTs) or plastid DNA (NUPTs); (iii) sequencing and characterization of mitochondrial and chloroplast genomes; (iv) recent advances in plastid genome engineering. Here we summarize the main findings of these works, which represent the latest research on the genetics, genomics, and biotechnology of chloroplasts and mitochondria.

scholarly journals Diversity of Cowpea [Vigna unguiculata (L.) Walp] Landraces in Mozambique: New Opportunities for Crop Improvement and Future Breeding Programs

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 991
Author(s):  
Ana Maria Figueira Gomes ◽  
David Draper ◽  
Nascimento Nhantumbo ◽  
Rafael Massinga ◽  
José C. Ramalho ◽  
...  
Keyword(s):  
Vigna Unguiculata ◽  
Production Systems ◽  
Crop Improvement ◽  
Morphological Diversity ◽  
Ecological Zones ◽  
Nutrition Security ◽  
Food And Nutrition ◽  
High Level ◽  
Nutrition Sensitive

Cowpea (Vigna unguiculata) is a neglected crop native to Africa, with an outstanding potential to contribute to the major challenges in food and nutrition security, as well as in agricultural sustainability. Two major issues regarding cowpea research have been highlighted in recent years—the establishment of core collections and the characterization of landraces—as crucial to the implementation of environmentally resilient and nutrition-sensitive production systems. In this work, we have collected, mapped, and characterized the morphological attributes of 61 cowpea genotypes, from 10 landraces spanning across six agro-ecological zones and three provinces in Mozambique. Our results reveal that local landraces retain a high level of morphological diversity without a specific geographical pattern, suggesting the existence of gene flow. Nevertheless, accessions from one landrace, i.e., Maringué, seem to be the most promising in terms of yield and nutrition-related parameters, and could therefore be integrated into the ongoing conservation and breeding efforts in the region towards the production of elite varieties of cowpea.

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