Synergism of antioxidant action of vitamins E, C and quercetin is related to formation of molecular associations in biomembranes

We investigated the antioxidant action of a solid preparation of Xingnaojing (XNJ) and ascorbic acid (AA) in stroke-prone spontaneously hypertensive rats (SHRSP). The total antioxidant states in the plasma, systolic blood pressure, and heart rate were measured every 2 weeks, and lipid peroxidation, expressed as thiobarbituric acid-reactive substances in plasma, was measured in the 6th week. The results showed that AA and XNJ significantly increased the total antioxidant status in plasma and reduced malondialdehyde in the plasma. These data suggest that during 6-week administration, XNJ has antioxidant action on SHRSP, which may relate to its generalized inhibition of lipid peroxidation and promotion of the total antioxidant state. These results demonstrated that orally treated XNJ has an antioxidant effect on SHRSP plasma.

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Effects of resveratrol on alpha-amanitin-induced nephrotoxicity in BALB/c mice

Human & Experimental Toxicology ◽

10.1177/0960327119888271 ◽

2019 ◽

Vol 39 (3) ◽

pp. 328-337

Author(s):

MA Arici ◽

A Sahin ◽

Z Cavdar ◽

BU Ergur ◽

C Ural ◽

...

Keyword(s):

Normal Saline ◽

Antioxidant Status ◽

Kidney Damage ◽

Amanita Phalloides ◽

Simultaneous Administration ◽

Antioxidant Action ◽

Treatment Groups ◽

Biochemical Analyses ◽

Alpha Amanitin ◽

Late Stages

Alpha-amanitin (α-AMA), the primary toxin of Amanita phalloides, is known to cause nephrotoxicity and hepatotoxicity. Resveratrol is an antioxidant that has shown efficacy in many nephrotoxicity models. The aim of this study was to investigate the effects of resveratrol against the early and late stages of α-AMA-induced nephrotoxicity, compared to those of silibinin, a well-known antidote for poisoning by α-AMA-containing mushrooms. Mice kidney tissues were obtained from five groups: (1) α-AMA + NS (simultaneous administration of α-AMA and normal saline), (2) α-AMA + SR (simultaneous administration of α-AMA and resveratrol), (3) α-AMA + 12R (resveratrol administration 12 h after α-AMA administration), (4) α-AMA + 24R (resveratrol administration 24 h after α-AMA administration), and (5) α-AMA + Sil (simultaneous administration of α-AMA and silibinin). Histomorphological and biochemical analyses were performed to evaluate kidney damage and oxidant–antioxidant status in the kidney. Scores of renal histomorphological damage decreased significantly in the early resveratrol treatment groups (α-AMA + SR and α-AMA + 12R), compared to those in the α-AMA + NS group ( p < 0.05). Catalase levels increased significantly in the α-AMA + SR group, compared to those in the α-AMA + NS group ( p < 0.001). Early resveratrol administration within 12 h after α-AMA ingestion may reverse the effects of α-AMA-induced nephrotoxicity, partly through its antioxidant action, thereby suggesting its potential as a treatment for poisoning by α-AMA-containing mushrooms.

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M.654 Antioxidant status and endothelium dysfunction markers in patients with diabetic retinopathy

Atherosclerosis ◽

10.1016/s0021-9150(04)90652-1 ◽

2004 ◽

Vol 5 (1) ◽

pp. 151

Author(s):

K SIEMIANOWICZ

Keyword(s):

Diabetic Retinopathy ◽

Antioxidant Status ◽

Endothelium Dysfunction

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Association between calcitriol and paricalcitol with oxidative stress in patients with hemodialysis

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000641 ◽

2020 ◽

pp. 1-8

Author(s):

Hasan Haci Yeter ◽

Berfu Korucu ◽

Elif Burcu Bali ◽

Ulver Derici

Keyword(s):

Oxidative Stress ◽

Vitamin D ◽

Antioxidant Status ◽

Total Antioxidant Status ◽

Stress Index ◽

Total Oxidant Status ◽

Vitamin D Analog ◽

Total Antioxidant ◽

Oxidant Status ◽

And Oxidative Stress

Abstract. Background: The pathophysiological basis of chronic kidney disease and its complications, including cardiovascular disease, are associated with chronic inflammation and oxidative stress. We investigated the effects of active vitamin D (calcitriol) and synthetic vitamin D analog (paricalcitol) on oxidative stress in hemodialysis patients. Methods: This cross-sectional study was composed of 83 patients with a minimum hemodialysis vintage of one year. Patients with a history of any infection, malignancy, and chronic inflammatory disease were excluded. Oxidative markers (total oxidant and antioxidant status) and inflammation markers (C-reactive protein and interleukin-6) were analyzed. Results: A total of 47% (39/83) patients were using active or analog vitamin D. Total antioxidant status was significantly higher in patients with using active or analog vitamin D than those who did not use (p = 0.006). Whereas, total oxidant status and oxidative stress index were significantly higher in patients with not using vitamin D when compared with the patients who were using vitamin D preparation (p = 0.005 and p = 0.004, respectively). On the other hand, total antioxidant status, total oxidant status, and oxidative stress index were similar between patients who used active vitamin D or vitamin D analog (p = 0.6; p = 0.4 and p = 0.7, respectively). Conclusion: The use of active or selective vitamin D analog in these patients decreases total oxidant status and increases total antioxidant status. Also, paricalcitol is as effective as calcitriol in decreasing total oxidant status and increasing total antioxidant status in patients with chronic kidney disease.

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Pro-oxidant effects of a high α-tocopherol dose on kidney antioxidant biomarkers and histopathological aspects

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000512 ◽

2017 ◽

Vol 87 (3-4) ◽

pp. 179-190

Author(s):

Amel Kanane ◽

Fayrouz Rouaki ◽

Mohamed Brahim Errahmani ◽

Abdenour Laraba ◽

Hayet Mesbah ◽

...

Keyword(s):

Sunflower Oil ◽

Antioxidant Status ◽

Basal Diet ◽

Inflammatory Cells ◽

Significant Rise ◽

Control Group ◽

Histopathological Changes ◽

Group 5 ◽

Oxidant Effect ◽

Higher Doses

Abstract. The aim of this study is to evaluate the effect of α-tocopherol supplementation at two doses (600 and 1200 mg × kg–1) on kidney antioxidant status and the histopathological changes in Wistar rats after 12 weeks of exposure at different diets. Forty rats has been divided into 4 groups of 10 rats each, the control group received basal diet with 5 % fresh sunflower oil (FSO), the second group: 5 % oxidized sunflower oil (OSO), the third group: 5 % OSO supplemented with 600 mg × kg–1 α-tocopherol and the fourth group: 5 % OSO supplemented with 1200 mg × kg–1 α-tocopherol. In OSO groups, the results showed highly significant increases of LPO (from 31.3 ± 0.9 to 53.8 ± 1.2 nmol of MDA formed/min/mg protein, p < 0.0001) with a significant decrease (p < = 0.001) of the antioxidant enzymatic activities (CAT, SOD, GPX, GR and G6PDH), body weight (339 ± 9 to 290 ± 3 g) and α-tocopherol levels (13.6 ± 0.6 to 6.5 ± 0.4 μg/mg protein). In OSO groups with 600 mg × kg–1 α-tocopherol, an antioxidant effect was found, reflected by a return of the parameters to values similar to those of the control group. However, higher doses of α-tocopherol (1200 mg × kg–1) induced a depletion of antioxidant status, α-tocopherol levels (6.0 ± 0.3 μg/mg protein, p < 0.001) and a very highly significant rise (p < 0.0001) of LPO content (54.86 ± 0.01 nmol of MDA formed/min/mg protein). The kidney tissues also showed changes in glomerular, severe inflammatory cells infiltration, and formation of novel vessels. So, we can conclude that the oxidative stress is attenuated by a moderate administration of 600 mg × kg–1 α-tocopherol, while a pro-oxidant effect occurs at 1200 mg × kg–1 α-tocopherol.

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