Biodegradation of heptadecane in hydrocarbon polluted dune sands using a newly-isolated thermophilic bacterium, Brevibacillus borstelensis TMU30: statistical evaluation and process optimization

RSC Advances ◽  
2015 ◽  
Vol 5 (42) ◽  
pp. 33414-33422 ◽  
Author(s):  
Bahram Khazra ◽  
Seyyed Mohammad Mousavi ◽  
Sadaf Mehrabi ◽  
Maryam Hashemi ◽  
Seyed Abbas Shojaosadati
Keyword(s):  
Process Optimization ◽  
Contaminated Soil ◽  
Statistical Evaluation ◽  
Petroleum Refinery ◽  
Enrichment Culture ◽  
Thermophilic Bacterium ◽  
Soil Samples ◽  
Dune Sands ◽  
Degrading Bacterium

An enrichment culture was established to isolate a thermophilic hydrocarbon-degrading bacterium from contaminated soil samples from the Tehran Petroleum Refinery.

scholarly journals An Investigation of Petrol Metabolizing Bacteria Isolated from Contaminated Soil Samples Collected from Various Fuel Stations

2019 ◽  
Vol 68 (2) ◽  
pp. 193-201 ◽  
Author(s):  
FATIMA MUCCEE ◽  
AMINA EJAZ
Keyword(s):  
Contaminated Soil ◽  
Phylogenetic Trees ◽  
High Efficiency ◽  
Thermophilic Bacteria ◽  
Enrichment Culture ◽  
Bacterial Species ◽  
Soil Samples ◽  
Biochemical Characteristics ◽  
Antibiotic Resistance Profile ◽  
Fermentation Test

The present study aimed to isolate the high-efficiency petrol metabolizing thermophilic bacteria from petrol contaminated soil samples. Isolation was carried out through enrichment culture, serial dilution and pour plate methods using the petrol supplemented minimal salt media. The isolated bacteria were analyzed to document growth behavior, petrol removal efficiencies, antibiotic resistance profile, and biochemical characteristics. The 16S rRNA based phylogenetic analysis helped to reveal the identity of isolated bacterial species and construct the phylogenetic trees. Total nine bacteria were isolated, out of which three (IUBP2, IUBP3, IUBP5) were identified as Brevibacillus formosus, one (IUBP1) was found similar to Brevibacillus agri, four (IUBP7, IUBP8, IUBP13, and IUBP14) shared homology with Burkholderia lata, and one (IUBP15) with Burkholderia pyrrocinia. All the isolates were fast growing and exhibited considerable petrol degradation potential. The highest petrol removal efficiency (69.5% ± 13.44/6 days) was recorded for the strain IUBP15 at a petrol concentration of 0.1% (v/v). All bacteria studied (100%) were positive for esculinase and phosphatase. Many strains exhibited positive responses for arginine dehydrolase (22%), β-naphthylamidase (11%), β-D-glucosaminide (33%), mannitol (55%), sorbitol (66%) and inulin (88%) fermentation test. While all were sensitive to the antibiotics, some of them were found resistant against chloramphenicol and oxacillin. The remarkable biochemical characteristics and considerable petrol removal potential (40–70%) highlights utilization of the bacteria isolated for petrol bioremediation, mineralization of organophosphates, dairy and food industry, and also as biofertilizers and biocontrol agents.

Lipopeptide biosurfactant production bacteria Acinetobacter sp. D3-2 and its biodegradation of crude oil

2014 ◽  
Vol 16 (4) ◽  
pp. 897-903 ◽  
Author(s):  
Mutai Bao ◽  
Yongrui Pi ◽  
Lina Wang ◽  
Peiyan Sun ◽  
Yiming Li ◽  
...  
Keyword(s):  
Crude Oil ◽  
Contaminated Soil ◽  
Potential Effect ◽  
Soil Samples ◽  
Biosurfactant Production ◽  
Degrading Bacterium ◽  
Petroleum Contaminated Soil ◽  
Lipopeptide Biosurfactant ◽  
Acinetobacter Sp

In this work, a hydrocarbon-degrading bacterium D3-2 isolated from petroleum contaminated soil samples was investigated for its potential effect in biodegradation of crude oil. The strain was identified as Acinetobacter sp.

High Parasitic Contamination of Soil Samples in the North of Iran: a Potential Risk of Parasitic Infection for Tourists

2020 ◽  
Vol 20 ◽  
Author(s):  
Beheshteh Haghparast-kenari ◽  
Tooran Nayeri ◽  
Shahabeddin Sarvi ◽  
Mohammad Taghi Rahimi ◽  
Ehsan Ahmadpour ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Urban Areas ◽  
Intestinal Parasites ◽  
Parasitic Infection ◽  
Animal Husbandry ◽  
Soil Samples ◽  
Human Society ◽  
Public Parks ◽  
The North ◽  
North Of Iran

Background: Soil-transmitted parasites (STPs) are significant intestinal parasites that infect humans and animals and impose considerable burdens on human society and animal husbandry industries. Therefore, the present study aimed to determine the prevalence of parasitic elements of soil samples collected from the north of Iran. Methods: A total of 256 soil samples were collected from public parks, public places, vegetable gardens, sand heaps, and shadow areas near houses in the north of Iran and examined using the sucrose flotation method. Results: Out of 256 examined samples, 131 (51.2%) ones showed parasitic contamination including larvae (43%), oocysts (14.1%), and different eggs (6.6%). According to the results, the most and least common parasites observed in the samples were larvae (43%), as well as Toxascaris leonina, and Trichuris trichiura (0.4%), respectively. Moreover, the most and least contaminated locations were sand heaps (62.5%) and shadow areas near houses (45.6%), respectively. Regarding cities, Behshahr (68.3%) and Sari (67.5%) had the highest contaminated soil samples, whereas Chalus (37.5%) showed the lowest contamination. On the other hand, rural samples showed more contamination, compared to urban areas (P< 0.05). Conclusion: The findings of the present study indicate that the overall prevalence of STPs in examined areas and highly contaminated soil samples can be considered as a potential source of human contamination particularly tourists with STPs.

Evaluation of Nanotechniques and Conventional Techniques for the Removal of Dioxins

2018 ◽  
Vol 9 (1) ◽  
pp. 79-84
Author(s):  
Vaishali V. Shahare ◽  
Rajni Grover ◽  
Suman Meena
Keyword(s):  
Soil Sample ◽  
Human Health ◽  
Surface Area ◽  
Contaminated Soil ◽  
Conventional Treatment ◽  
Volume Ratio ◽  
Soil Samples ◽  
Dioxins And Furans ◽  
Palladized Iron ◽  
The Impact

Background: The persistent dioxins/furans has caused a worldwide concern as they influence the human health. Recent research indicates that nonmaterial may prove effective in the degradation of Dioxins/furans. The nanomaterials are very reactive owing to their large surface area to volume ratio and large number of reactive sites. However, nanotechnology applications face both the challenges and the opportunities to influence the area of environmental protection. Objective: i) To study the impact of oil mediated UV-irradiations on the removal of 2,3,7,8-TCDD, 2,3,7,8-TCDF, OCDD and OCDF in simulated soil samples. ii) To compare the conventional treatment methods with the modern available nanotechniques for the removal of selected Dioxins/furans from soil samples. Methods: The present work has investigated an opportunity of the degradation of tetra and octachlorinated dioxins and furans by using oil mediated UV radiations with subsequent extraction of respective dioxins/furans from soils. The results have been compared with the available nanotechniques. Results: The dioxin congeners in the simulated soil sample showed decrease in concentration with the increase in the exposure time and intensity of UV radiations. The dechlorination of PCDD/Fs using palladized iron has been found to be effective. Conclusion: Both the conventional methods and nanotechnology have a dramatic impact on the removal of Dioxins/furans in contaminated soil. However, the nanotechniques are comparatively costlier and despite the relatively high rates of PCDDs dechlorination by Pd/nFe, small fraction of the dioxins are recalcitrant to degradation over considerable exposure times.

scholarly journals Identifying Hidden Viable Bacterial Taxa in Tropical Forest Soils Using Amplicon Sequencing of Enrichment Cultures

Biology ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 569
Author(s):  
Chakriya Sansupa ◽  
Sara Fareed Mohamed Wahdan ◽  
Terd Disayathanoowat ◽  
Witoon Purahong
Keyword(s):  
Bacterial Community ◽  
Forest Soil ◽  
Culture Media ◽  
Enrichment Culture ◽  
Sequence Similarity ◽  
Illumina Miseq ◽  
Amplicon Sequencing ◽  
Soil Samples ◽  
Enrichment Cultures ◽  
Total Community

This study aims to estimate the proportion and diversity of soil bacteria derived from eDNA-based and culture-based methods. Specifically, we used Illumina Miseq to sequence and characterize the bacterial communities from (i) DNA extracted directly from forest soil and (ii) DNA extracted from a mixture of bacterial colonies obtained by enrichment cultures on agar plates of the same forest soil samples. The amplicon sequencing of enrichment cultures allowed us to rapidly screen a culturable community in an environmental sample. In comparison with an eDNA community (based on a 97% sequence similarity threshold), the fact that enrichment cultures could capture both rare and abundant bacterial taxa in forest soil samples was demonstrated. Enrichment culture and eDNA communities shared 2% of OTUs detected in total community, whereas 88% of enrichment cultures community (15% of total community) could not be detected by eDNA. The enrichment culture-based methods observed 17% of the bacteria in total community. FAPROTAX functional prediction showed that the rare and unique taxa, which were detected with the enrichment cultures, have potential to perform important functions in soil systems. We suggest that enrichment culture-based amplicon sequencing could be a beneficial approach to evaluate a cultured bacterial community. Combining this approach together with the eDNA method could provide more comprehensive information of a bacterial community. We expected that more unique cultured taxa could be detected if further studies used both selective and non-selective culture media to enrich bacteria at the first step.

scholarly journals Factorial design study of total petroleum contaminated soil treatment using land farming technique

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Ehizonomhen S. Okonofua ◽  
Kayode H. Lasisi ◽  
Eguakhide Atikpo
Keyword(s):  
Moisture Content ◽  
Factorial Design ◽  
Contaminated Soil ◽  
Soil Samples ◽  
Optimization Techniques ◽  
Cow Dung ◽  
Optimum Point ◽  
Input Parameters ◽  
Substrate Moisture ◽  
Land Farming

AbstractLand farming technique was used to treat hydrocarbon contaminated soil collected from a crude oil spill sites in Edo State, Nigeria. Calibrated standard auger was used to collect soil samples from the site at depth below 30 cm. The samples were characterized and classified. Cow dung and NPK fertilizer were added as additives to complement the nutriments of the soil samples before total petroleum hydrocarbon (TPH) quantification and remediation procedures. Factorial design was applied to vary the input parameters such as pH, mass of substrate, moisture content and turning times of land farming so to ascertain the optimal conditions for the procedure. The result revealed that the in-situ TPH value was 5000 mg kg− 1 on the average and after 90 d of treatment, TPH reduced to 646 mg kg− 1. The turning rate, pH, moisture content and mass of substrate hade 83, 4.36, 0.48 and 0.046% contribution, respectively, for the degradation process using land farming treatment. Numerical optimization techniques applied in the optimum point for land farming input parameters to achieve predicted maximum removal of 99% were evaluated as pH, mass of substrate, moisture content and turning rate to be 6.01, 1 kg, 10% and 5 times in a week, respectively. TPH removed at this optimum point was 98% reducing from 5000 to 636 mg kg− 1. The high coefficient of determination (r2 = 0.9865) as observed in the closeness of predicted and experimental values reflects the reliability of the model and hence, land farming practice with close attention on turning rate as revealed by this study, is recommended for TPH contaminated soil remediation.

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