scholarly journals An Investigation of Petrol Metabolizing Bacteria Isolated from Contaminated Soil Samples Collected from Various Fuel Stations

2019 ◽  
Vol 68 (2) ◽  
pp. 193-201 ◽  
Author(s):  
FATIMA MUCCEE ◽  
AMINA EJAZ
Keyword(s):  
Contaminated Soil ◽  
Phylogenetic Trees ◽  
High Efficiency ◽  
Thermophilic Bacteria ◽  
Enrichment Culture ◽  
Bacterial Species ◽  
Soil Samples ◽  
Biochemical Characteristics ◽  
Antibiotic Resistance Profile ◽  
Fermentation Test

The present study aimed to isolate the high-efficiency petrol metabolizing thermophilic bacteria from petrol contaminated soil samples. Isolation was carried out through enrichment culture, serial dilution and pour plate methods using the petrol supplemented minimal salt media. The isolated bacteria were analyzed to document growth behavior, petrol removal efficiencies, antibiotic resistance profile, and biochemical characteristics. The 16S rRNA based phylogenetic analysis helped to reveal the identity of isolated bacterial species and construct the phylogenetic trees. Total nine bacteria were isolated, out of which three (IUBP2, IUBP3, IUBP5) were identified as Brevibacillus formosus, one (IUBP1) was found similar to Brevibacillus agri, four (IUBP7, IUBP8, IUBP13, and IUBP14) shared homology with Burkholderia lata, and one (IUBP15) with Burkholderia pyrrocinia. All the isolates were fast growing and exhibited considerable petrol degradation potential. The highest petrol removal efficiency (69.5% ± 13.44/6 days) was recorded for the strain IUBP15 at a petrol concentration of 0.1% (v/v). All bacteria studied (100%) were positive for esculinase and phosphatase. Many strains exhibited positive responses for arginine dehydrolase (22%), β-naphthylamidase (11%), β-D-glucosaminide (33%), mannitol (55%), sorbitol (66%) and inulin (88%) fermentation test. While all were sensitive to the antibiotics, some of them were found resistant against chloramphenicol and oxacillin. The remarkable biochemical characteristics and considerable petrol removal potential (40–70%) highlights utilization of the bacteria isolated for petrol bioremediation, mineralization of organophosphates, dairy and food industry, and also as biofertilizers and biocontrol agents.

scholarly journals Bacterial Separation and Community Diversity Analysis of Petroleum Contaminated Soil in Yumen Oilfield

2020 ◽  
Vol 71 (3) ◽  
pp. 595-607
Author(s):  
Yonggang Wang ◽  
Shengcai Dou ◽  
Qingfang Zhang ◽  
Abdolghaffar Ebadi ◽  
Jixiang Chen ◽  
...  
Keyword(s):  
Microbial Diversity ◽  
Contaminated Soil ◽  
High Efficiency ◽  
Oil Pollution ◽  
Soil Samples ◽  
Desert Soil ◽  
Western China ◽  
Desert Area ◽  
Soil Microbial ◽  
Degrading Bacteria

The problem of environmental pollution caused by the development and use of petroleum is increasingly obvious, which is a serious threat to human health. The use of microbial degradation to treat oil pollution is one of the environmentally effective, economical and practical methods.In order to explore the soil microbial diversity in the desert area of Northwest China, this paper analyzes the soil bacterial diversity of soil samples collected from different oil-contaminated areas in Yumen Oilfield for the oil pollution problem in the Yumen Oilfield in the northwest desert area, and selects the high efficiency through pure culture technology. Petroleum degradation bacteria, and research on the biological characteristics of degrading bacteria. The composition, abundance and diversity of bacterial communities in oil-contaminated soil in Yumen Oilfield were analyzed. The culturable bacteria in western oil-contaminated desert soil were separated by coating plate method. The bacterial morphology and 16S rRNA gene system development analysis were studied. The structure and diversity of bacterial community could be cultured, and the oil utilization and degradation ability of the strain could be analyzed. The microbial diversity of Yumen oil-contaminated desert soil was analyzed by Illumina Miseq high-throughput sequencing. Through research, it is found that there are abundant bacterial groups in the oil-contaminated desert soil, and there are obvious diversity. The genetic material in the variable regions of the six soil samples detected a total of 3943 0TU at 97% similarity level, and obtained the soil microbial community. Doors, 48 classes, 78 orders, 179 families and 471 genera, including most common high-efficiency petroleum-degrading bacteria. Petroleum hydrocarbon pollution can change the microbial diversity and community structure of the original soil. The size of microbial diversity in the six soil samples is B2]A1]B1]A2]C1]C2, the diversity of B2 is the highest, the diversity of C2 is the lowest, and the microbial diversity differed greatly between groups, and there was no difference in the group. Among the dominant bacteria isolated from contaminated soil, 8 strains of oil have a degradation rate of more than 30%, including the species of the genus Rhodococcus and Pseudomonas. Soil desertification in western China has a great impact on the local ecological environment. Studying the microbial diversity of desert soils and separating high-efficiency petroleum-degrading strains is of great significance for strengthening the ecological restoration of oil-contaminated environment in desert areas.

scholarly journals Identifying an efficient bacterial species and its genetic erosion for arsenic bioremediation of gold mining soil

2016 ◽  
Vol 42 (3) ◽  
pp. 58-66 ◽  
Author(s):  
Lamyai Neeratanaphan ◽  
Tawatchai Tanee ◽  
Alongklod Tanomtong ◽  
Bundit Tengjaroenkul
Keyword(s):  
Contaminated Soils ◽  
Gold Mining ◽  
High Efficiency ◽  
Bacterial Species ◽  
Genetic Erosion ◽  
Morphological Characteristics ◽  
Soil Samples ◽  
Rrna Gene ◽  
Mining Areas ◽  
Mining Soil

Abstract To improve bioremediation of arsenic (As) contamination in soil, the use of microorganisms to efficiently reduce As and their assessment of genetic erosion by DNA damage using genomic template stability (GTS) evaluation and using RAPD markers were investigated. The five sites examined for microorganisms and contaminated soils were collected from affected gold mining areas. The highest As concentration in gold mining soil is 0.72 mg/kg. Microorganism strains isolated from the gold mining soil samples were tested for As removal capacity. Two bacterial isolates were identified by 16S rRNA gene sequence analysis and morphological characteristics as Brevibacillus reuszeri and Rhodococcus sp. The ability to treat As in nutrient agar (NA) at 1,600 mg/L and contaminated soil samples at 0.72 mg/kg was measured at 168 h, revealing more efficient As removal by B. reuszeri than Rhodococcus sp. (96.67% and 94.17%, respectively). Both species have the capacity to remove As, but B. reuszeri shows improved growth compared to the Rhodococcus sp. B. reuszeri might be suitable for adaptation and use in As treatment. The results are in agreement with their genetic erosion values, with B. reuszeri showing very little genetic erosion (12.46%) of culture in As concentrations as high as 1,600 mg/L, whereas 82.54% genetic erosion occurred in the Rhodococcus sp., suggesting that Rhodococcus sp. would not survive at this level of genetic erosion. Therefore, B. reuszeri has a high efficiency and can be used for soil As treatment, as it is capable to tolerate a concentration of 0.72 mg/kg and as high as 1,600 mg/L in NA.

Study of Ultraviolet Spectrophotometer for Rapid Analysis of Oil Content in the Oil-Contaminated Soil

2013 ◽  
Vol 864-867 ◽  
pp. 930-934 ◽  
Author(s):  
Li Hua Mao ◽  
Xue Mei Han
Keyword(s):  
Contaminated Soil ◽  
Room Temperature ◽  
Oil Content ◽  
High Efficiency ◽  
Extraction Efficiency ◽  
Detection Method ◽  
Ultrasonic Method ◽  
Rapid Analysis ◽  
Soil Samples ◽  
Two Stages

Oil soil samples were extracted using cyclohexane, petroleum ether and chloroformion by the ultrasonic method. The feasibility of the ultraviolet spectrophotometric method for rapid analysis of the oil content in the oil-contaminated soil was explored. The result indicates that compared with other test methodsultraviolet spectrophotometry can be a simple, quick and steady detection method of oil content in the oil-contaminated soil. The method of oil material extracted by cyclohexane is high efficiency and stable. When the temperature was between room temperature and 40°C, ultrasonic extraction time is 15 min, and the extraction efficiency turned out to be stable (greater than 94%) when solvent dosage exceeded 40mL. Two stages extraction could achieve the best effect with the extraction dosage of 20 mL for each stage.

scholarly journals Biodegradation of spent engine oil by bacteria isolated from the rhizosphere of legumes grown in contaminated soil

2014 ◽  
Vol 3 (2) ◽  
pp. 63-75 ◽  
Author(s):  
HY Ismail ◽  
UJJ Ijah ◽  
ML Riskuwa ◽  
II Allamin
Keyword(s):  
Molecular Weight ◽  
Contaminated Soil ◽  
Rhizosphere Soil ◽  
Enrichment Culture ◽  
Bacterial Species ◽  
Engine Oil ◽  
Klebsiella Pneumonia ◽  
Lablab Purpureus ◽  
Degrading Bacteria ◽  
Spent Engine Oil

Biodegradation of spent engine oil (SEO) by bacteria isolated from the rhizosphere of Cajan cajan and Lablab purpureus was investigated. It was with a view to determining most efficient bacterial species that could degrade SEO in phytoremediation studies. Hydrocarbon degrading bacteria were isolated and identified by enrichment culture technique using oil agar supplemented with 0.1% v/v SEO. Total heterotrophic and oil utilizing bacterial count showed the occurrence of large number of bacteria predominantly in the rhizosphere soil, ranging between 54×108 - 144×108 CFU/g and 4×108- 96×108 CFU/g respectively. Percentage of oil utilizing bacteria ranged between 0% (uncontaminated non rhizosphere soil) to 76% (contaminated rhizosphere). Turbidimetrically, five bacterial species namely Pseudomonas putrefacience CR33, Klebsiella pneumonia CR23, Pseudomonas alcaligenes LR14, Klebsiella aerogenes CR21, and Bacillus coagulans CR31 were shown to grow maximally and degraded the oil at the rate of 68%, 62%, 59%, 58%and 45% respectively. Chromatographic analysis using GC-MS showed the presence of lower molecular weight hydrocarbons in the residual oil (indicating degradation) after 21 days, whereas the undegraded oil (control) had higher molecular weight hydrocarbons after the same period. The species isolated were shown to have high ability of SEO biodegradation and therefore could be important tools in ameliorating SEO contaminated soil. DOI: http://dx.doi.org/10.3126/ije.v3i2.10515 International Journal of the Environment Vol.3(2) 2014: 63-75

scholarly journals Biodegradation of used Engine Oil Contaminated Site using Individual and Mixed bacterial isolates

2020 ◽  
pp. 38-45
Author(s):  
Muh'd A. J. ◽  
Sa'adatu A.Y. ◽  
Surayya M. M. ◽  
Sa'adatu M. I. ◽  
Nafisa B. ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Contaminated Soil ◽  
Bacterial Species ◽  
Soil Samples ◽  
Engine Oil ◽  
Contaminated Site ◽  
Bacterial Isolates ◽  
The World ◽  
Degradation Ability

Oil is most widely distributed source of energy in the world and large-scaled environmental pollutant. Oil, oil products, and oil containing industrial waste pollution is ranked second place after radioactive pollution on account of their harmful action to ecosystems. Contamination of soil by organic chemicals (mostly hydrocarbons) is prevalent in oil producing and industrialized countries of the world. Biodegradation, a strategy that uses biological means (i.e microbes) to degrade, stabilize and remove soil contaminants is an alternative green technology remediation of hydrocarbon contaminated soil. This study was carried out to isolate and screen Bacteria capable of degrading used engine oil from oil contaminated site. Soil samples were collected from oil contaminated site. Bacterial species were isolated from the collected soil samples by serial dilution and agar methods. Different bacterial species were isolated but only four were oil degrading isolates. The identity of the various genera of bacterial contaminants were determined by a combination of cultural, morphological as well as preliminary biochemical characteristics of the isolates. The four oil degrading bacteria (AJ1, AJ2, AJ3 and AJ4) were preliminarily identified as Bacillus cereus, Pseudomonas aeruginosa, Bacillus subtillis and Micrococcus spp respectively. The degradation ability of the bacterial isolates was screened and maximum degradation was recorded by AJ 5 (Mixed culture) with 66.9 %, followed by Bacillus cereus (50.3 %), Bacillus subtilis (44 %), Pseudomonas aeruginosa (37.9 %) and the least was seen in Micrococcus spp (35.3 %). These findings revealed that some bacteria species are capable of utilizing the oil and used it as sole source of carbon and energy and the mixed consortia of the bacteria have rapid degradation ability. Biological degradation of hydrocarbon contaminated soil offers a better and more environmentally friendly technique that if properly explored can bring our environment into a better place for both plant and animal.

Biodegradation of heptadecane in hydrocarbon polluted dune sands using a newly-isolated thermophilic bacterium, Brevibacillus borstelensis TMU30: statistical evaluation and process optimization

RSC Advances ◽  
2015 ◽  
Vol 5 (42) ◽  
pp. 33414-33422 ◽  
Author(s):  
Bahram Khazra ◽  
Seyyed Mohammad Mousavi ◽  
Sadaf Mehrabi ◽  
Maryam Hashemi ◽  
Seyed Abbas Shojaosadati
Keyword(s):  
Process Optimization ◽  
Contaminated Soil ◽  
Statistical Evaluation ◽  
Petroleum Refinery ◽  
Enrichment Culture ◽  
Thermophilic Bacterium ◽  
Soil Samples ◽  
Dune Sands ◽  
Degrading Bacterium

An enrichment culture was established to isolate a thermophilic hydrocarbon-degrading bacterium from contaminated soil samples from the Tehran Petroleum Refinery.

Sidrophore Production and Phosphate Solubilization by Bacillus cereus and Pseudomonas fluorescens Isolated from Iraqi Soils and Soil Characterization

2018 ◽  
Vol 9 (4) ◽  
Author(s):  
Zaid Raad Abbas ◽  
Aqeel Mohammed Majeed Al-Ezee ◽  
Sawsan H
Keyword(s):  
Bacillus Cereus ◽  
Pseudomonas Fluorescens ◽  
Phosphate Solubilization ◽  
Bacterial Species ◽  
Nutrient Status ◽  
Bacterial Count ◽  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Clear Zone ◽  
Zone Formation

This study was conducted to explore the ability of Pseudomonas fluorescens and Bacillus cereus to solubilizing a phosphate in soil for enhancing the planting growth and, its relation with soill characterization. The isolates were identified as P.fluorescens and B. cereus using convential analysis and, its phosphate solubilization ability and sidrophore was shown by the clear zone formation on National Botanical Research Institute���s Phosphate medium. Moreover, Pseudomonas fluorescens isolates (n = 9) and three of B. cereus isolated from agricultural area in Baghdad university, Mustansiriyah university and Diyala bridge. Results displayed that bacterial count were varied in soil samples according to their region, and ranging from 30 to 60 *10 2 CFU/g in Baghdad university soil to 10���20 *10 2 CFU/g in Mustansiriyah university soil, the Baghdad soil macronutrient which included: NH4, NO3, P, and K were, 8.42, 20.53, 19.09, 218.73 respectively, While the physio analysis revealed that the mean of pH was 7.3 and EC was 8.63. on the other hand the micronutrient analysis indicated that the soil samples were included Ca, Fe, Mn, Zn and Cu which gave their mean 5025.9, 8.9, 4.9, 0.5 and 1.5 respectevily. Results revealed that all isolated bacteria (9 isolates of P.fluorescens and three isolates of B. cereus gave ahalo zone which mean their ability to be phosphate solubilizing bacteria at 100%. Results revealed that all isolated bacteria were detected a ability to produce high levels from chelating agents (siderophores)) by P.fluorescens and. B cereus at 100%, when appeared ahalo clear zone. Furthermore, the high levels of phosphate solubilization and siderophore production were grouped in bacterial species isolated from Iraqi soils. might be attributed to many soil factors such as soil nutrient status, soil acidity, water content, organic matter and soil enzyme activities.

High Parasitic Contamination of Soil Samples in the North of Iran: a Potential Risk of Parasitic Infection for Tourists

2020 ◽  
Vol 20 ◽  
Author(s):  
Beheshteh Haghparast-kenari ◽  
Tooran Nayeri ◽  
Shahabeddin Sarvi ◽  
Mohammad Taghi Rahimi ◽  
Ehsan Ahmadpour ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Urban Areas ◽  
Intestinal Parasites ◽  
Parasitic Infection ◽  
Animal Husbandry ◽  
Soil Samples ◽  
Human Society ◽  
Public Parks ◽  
The North ◽  
North Of Iran

Background: Soil-transmitted parasites (STPs) are significant intestinal parasites that infect humans and animals and impose considerable burdens on human society and animal husbandry industries. Therefore, the present study aimed to determine the prevalence of parasitic elements of soil samples collected from the north of Iran. Methods: A total of 256 soil samples were collected from public parks, public places, vegetable gardens, sand heaps, and shadow areas near houses in the north of Iran and examined using the sucrose flotation method. Results: Out of 256 examined samples, 131 (51.2%) ones showed parasitic contamination including larvae (43%), oocysts (14.1%), and different eggs (6.6%). According to the results, the most and least common parasites observed in the samples were larvae (43%), as well as Toxascaris leonina, and Trichuris trichiura (0.4%), respectively. Moreover, the most and least contaminated locations were sand heaps (62.5%) and shadow areas near houses (45.6%), respectively. Regarding cities, Behshahr (68.3%) and Sari (67.5%) had the highest contaminated soil samples, whereas Chalus (37.5%) showed the lowest contamination. On the other hand, rural samples showed more contamination, compared to urban areas (P< 0.05). Conclusion: The findings of the present study indicate that the overall prevalence of STPs in examined areas and highly contaminated soil samples can be considered as a potential source of human contamination particularly tourists with STPs.

Evaluation of Nanotechniques and Conventional Techniques for the Removal of Dioxins

2018 ◽  
Vol 9 (1) ◽  
pp. 79-84
Author(s):  
Vaishali V. Shahare ◽  
Rajni Grover ◽  
Suman Meena
Keyword(s):  
Soil Sample ◽  
Human Health ◽  
Surface Area ◽  
Contaminated Soil ◽  
Conventional Treatment ◽  
Volume Ratio ◽  
Soil Samples ◽  
Dioxins And Furans ◽  
Palladized Iron ◽  
The Impact

Background: The persistent dioxins/furans has caused a worldwide concern as they influence the human health. Recent research indicates that nonmaterial may prove effective in the degradation of Dioxins/furans. The nanomaterials are very reactive owing to their large surface area to volume ratio and large number of reactive sites. However, nanotechnology applications face both the challenges and the opportunities to influence the area of environmental protection. Objective: i) To study the impact of oil mediated UV-irradiations on the removal of 2,3,7,8-TCDD, 2,3,7,8-TCDF, OCDD and OCDF in simulated soil samples. ii) To compare the conventional treatment methods with the modern available nanotechniques for the removal of selected Dioxins/furans from soil samples. Methods: The present work has investigated an opportunity of the degradation of tetra and octachlorinated dioxins and furans by using oil mediated UV radiations with subsequent extraction of respective dioxins/furans from soils. The results have been compared with the available nanotechniques. Results: The dioxin congeners in the simulated soil sample showed decrease in concentration with the increase in the exposure time and intensity of UV radiations. The dechlorination of PCDD/Fs using palladized iron has been found to be effective. Conclusion: Both the conventional methods and nanotechnology have a dramatic impact on the removal of Dioxins/furans in contaminated soil. However, the nanotechniques are comparatively costlier and despite the relatively high rates of PCDDs dechlorination by Pd/nFe, small fraction of the dioxins are recalcitrant to degradation over considerable exposure times.

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