Effective immobilization of silver nanoparticles on a regenerated cellulose–chitosan composite membrane and its antibacterial activity

Silver nanoparticles (Ag-NPs) possess excellent antibacterial properties and are considered to be an alternative material for treating antibiotic-resistant bacteria. The present study was aimed at enhancing the antibacterial efficiency of Ag-NPs using visible laser light against Escherichia coli and Staphylococcus aureus in vitro. Four concentrations of Ag-NPs (12.5, 25, 50, and 100 μg/ml), synthesized by the chemical reduction method, were utilized to conduct the antibacterial activity of prepared Ag-NPs. The antibacterial efficiencies of photoactivated Ag-NPs against both bacteria were determined by survival assay after exposure to laser irradiation. The mechanism of interactions between Ag-NPs and the bacterial cell membranes was then evaluated via scanning electron microscopy (SEM) and reactive oxygen species analysis to study the cytotoxic action of photoactivated Ag-NPs against both bacterial species. Results showed that the laser-activated Ag-NP treatment reduced the surviving population to 14% of the control in the E. coli population, while the survival in the S. aureus population was reduced to 28% of the control upon 10 min exposure time at the concentration of 50 μg/ml. However, S. aureus showed lower sensitivity after photoactivation compared to E. coli. Moreover, the effects depended on the concentration of Ag-NPs and exposure time to laser light. SEM images of treated bacterial cells indicated that substantial morphological changes occurred in cell membranes after treatment. The results suggested that Ag-NPs in the presence of visible light exhibit strong antibacterial activity which could be used to inactivate harmful and pathogenic microorganisms.

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BACTERIAL CELLULOSE FROM RICE WASTE WATER AND ITS COMPOSITE WHICH ARE DEPOSITED NANOPARTICLE AS AN ANTIMICROBIAL MATERIAL

ALCHEMY Jurnal Penelitian Kimia ◽

10.20961/alchemy.v12i1.946 ◽

2016 ◽

Vol 12 (1) ◽

pp. 70 ◽

Cited By ~ 1

Author(s):

Eli Rohaeti ◽

Endang W Laksono ◽

Anna Rakhmawati

Keyword(s):

Waste Water ◽

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Antibacterial Activity ◽

Bacterial Cellulose ◽

Nitrate Solution ◽

Diffusion Method ◽

E Coli ◽

Vis Spectroscopy ◽

Chitosan Composite

<pre>Bacterial cellulose (C) and its composites were synthesized from rice waste water with addition of glycerol (G) and chitosan (Ch).Antibacterial activity of the C, the bacterial cellulose-chitosan composite (CCh), and the bacterial cellulose – glycerol - chitosan composite (CGCh) which were deposited silver nanoparticles against S. aureus, E. coli, and yeast C. albicans has been conducted. Silver nanoparticles was prepared by chemical reduction of a silver nitrate solution, a trisodium citrate as a reductor, and a PVA as a stabilizer. The UV-Vis spectroscopy is used to determine the formation of silver nanoparticles. The characterization was conducted on the bacterial celluloses and those composites including the functional groups by the FTIR, the mechanical properties by Tensile Tester, photos surfaces by SEM, and the test of the antibacterial activity against S. aureus, E. coli, and C. albicans by diffusion method. The silver nanoparticle characterization indicates that the silver nanoparticles are formed at a wavelength of 418.80 nm. The antibacterial test showed an inhibitory effect of the C, the CCh, and the CGCh which are deposited the silver nanoparticles against of S. aureus, E. coli, and C.albicans. The CGChs which are deposited silver nanoparticles has the highest antimicrobial activity against the Staphylococcus aureus ATCC 25923. The CGs which are deposited silver nanoparticles provide the highest antimicrobial activity against the E. coli ATCC 25922 and the yeast Candida albicans ATCC 10231.</pre>

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One-Step Synthesis of Silver Nanoparticles Embedded Polyurethane Nano-Fiber/Net Structured Membrane as an Effective Antibacterial Medium

Polymers ◽

10.3390/polym11071185 ◽

2019 ◽

Vol 11 (7) ◽

pp. 1185 ◽

Cited By ~ 9

Author(s):

Bishweshwar Pant ◽

Mira Park ◽

Soo-Jin Park

Keyword(s):

Silver Nanoparticles ◽

Electrospinning Process ◽

Wound Dressings ◽

Ag Nps ◽

E Coli ◽

Antibacterial Performance ◽

Nano Fiber ◽

One Step ◽

Preparation Route ◽

Nanofiber Mats

A new and straightforward route was proposed to incorporate silver nanoparticles (Ag NPs) into the surface of polyurethane nanofibers (PU NFs). Uniform distribution of in situ formed Ag NPs on the surface of PU NFs was achieved by adding AgNO3 and tannic acid in a PU solution prior to the electrospinning process. The synthesized nanofiber mats were characterized with state-of-the-art techniques and antibacterial performances were tested against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) bacteria. The cytocompatibility and cell behavior were studied by using fibroblast cells. Following this preparation route, Ag/PU NFs can be obtained with excellent antibacterial performance, thus making them appropriate for various applications such as water filtration, wound dressings, etc.

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BACTERIAL CELLULOSE FROM RICE WASTE WATER AND ITS COMPOSITE WHICH ARE DEPOSITED NANOPARTICLE AS AN ANTIMICROBIAL MATERIAL

ALCHEMY Jurnal Penelitian Kimia ◽

10.20961/alchemy.12.1.946.70-87 ◽

2016 ◽

Vol 12 (1) ◽

pp. 70 ◽

Cited By ~ 2

Author(s):

Eli Rohaeti ◽

Endang W Laksono ◽

Anna Rakhmawati

Keyword(s):

Waste Water ◽

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Antibacterial Activity ◽

Bacterial Cellulose ◽

Nitrate Solution ◽

Diffusion Method ◽

E Coli ◽

Vis Spectroscopy ◽

Chitosan Composite

<pre>Bacterial cellulose (C) and its composites were synthesized from rice waste water with addition of glycerol (G) and chitosan (Ch).Antibacterial activity of the C, the bacterial cellulose-chitosan composite (CCh), and the bacterial cellulose – glycerol - chitosan composite (CGCh) which were deposited silver nanoparticles against S. aureus, E. coli, and yeast C. albicans has been conducted. Silver nanoparticles was prepared by chemical reduction of a silver nitrate solution, a trisodium citrate as a reductor, and a PVA as a stabilizer. The UV-Vis spectroscopy is used to determine the formation of silver nanoparticles. The characterization was conducted on the bacterial celluloses and those composites including the functional groups by the FTIR, the mechanical properties by Tensile Tester, photos surfaces by SEM, and the test of the antibacterial activity against S. aureus, E. coli, and C. albicans by diffusion method. The silver nanoparticle characterization indicates that the silver nanoparticles are formed at a wavelength of 418.80 nm. The antibacterial test showed an inhibitory effect of the C, the CCh, and the CGCh which are deposited the silver nanoparticles against of S. aureus, E. coli, and C.albicans. The CGChs which are deposited silver nanoparticles has the highest antimicrobial activity against the Staphylococcus aureus ATCC 25923. The CGs which are deposited silver nanoparticles provide the highest antimicrobial activity against the E. coli ATCC 25922 and the yeast Candida albicans ATCC 10231.</pre>

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BACTERIAL CELLULOSE FROM RICE WASTE WATER AND ITS COMPOSITE WHICH ARE DEPOSITED NANOPARTICLE AS AN ANTIMICROBIAL MATERIAL

ALCHEMY Jurnal Penelitian Kimia ◽

10.20961/alchemy.v12i1.788 ◽

2016 ◽

Vol 12 (1) ◽

Author(s):

Eli Rohaeti

Keyword(s):

Waste Water ◽

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Antibacterial Activity ◽

Bacterial Cellulose ◽

Nitrate Solution ◽

Diffusion Method ◽

E Coli ◽

Vis Spectroscopy ◽

Chitosan Composite

<pre>Bacterial cellulose (C) and its composites were synthesized from rice waste water with addition of glycerol (G) and chitosan (Ch).Antibacterial activity of the C, the bacterial cellulose-chitosan composite (CCh), and the bacterial cellulose – glycerol - chitosan composite (CGCh) which were deposited silver nanoparticles against S. aureus, E. coli, and yeast C. albicans has been conducted. Silver nanoparticles was prepared by chemical reduction of a silver nitrate solution, a trisodium citrate as a reductor, and a PVA as a stabilizer. The UV-Vis spectroscopy is used to determine the formation of silver nanoparticles. The characterization was conducted on the bacterial celluloses and those composites including the functional groups by the FTIR, the mechanical properties by Tensile Tester, photos surfaces by SEM, and the test of the antibacterial activity against S. aureus, E. coli, and C. albicans by diffusion method. The silver nanoparticle characterization indicates that the silver nanoparticles are formed at a wavelength of 418.80 nm. The antibacterial test showed an inhibitory effect of the C, the CCh, and the CGCh which are deposited the silver nanoparticles against of S. aureus, E. coli, and C.albicans. The CGChs which are deposited silver nanoparticles has the highest antimicrobial activity against the Staphylococcus aureus ATCC 25923. The CGs which are deposited silver nanoparticles provide the highest antimicrobial activity against the E. coli ATCC 25922 and the yeast Candida albicans ATCC 10231.</pre>

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Comparison of the antibacterial activity against Escherichia coli of silver nanoparticle produced by chemical synthesis with biosynthesis

Materials Science Materials Review ◽

10.18063/msmr.v2i2.679 ◽

2018 ◽

Vol 2 (2) ◽

Author(s):

Nguyen Phuc Quan 1,2 ◽

Tran Quoc Vinh 1 ◽

Kieu Thi My Yen 1 ◽

Le Vu Khanh Trang 2 ◽

Nguyen Minh Ly 2 ◽

...

Keyword(s):

Escherichia Coli ◽

Silver Nanoparticles ◽

Antibacterial Activity ◽

Color Change ◽

Chemical Reduction ◽

Spherical Shape ◽

Average Diameter ◽

Ag Nps ◽

E Coli ◽

Vis Spectroscopy

The synthesis of silver nanoparticles (Ag NPs) has been carried out using different methods, mainly by biological and chemical methods; however, comparing antibacterial activity of Ag NPs synthesized by these methods has not been conducted before. In this study, silver nanoparticles (Ag NPs) were synthesized by methods using reducing agent NaBH4/carboxymethyl cellulose (CMC) and fungal strain Trichoderma asperellum (T.asperellum). The formation of silver nanoparticles was observed visually by color change and identified by Ultraviolet-visible (UV – vis) spectroscopy. The transmission electron microscopy (TEM) image illustrated almost nanoparticles with spherical shape and average diameter of 4.1 ± 0.2 nm and 2.1 ± 0.2 nm of samples produced from chemical reduction and biosynthesis respectively. Both samples after 180 days storing have been separated lightly, but the agglomeration and absorbance peak shifting were not observed which proved the high stability of synthesized Ag NPs. Antimicrobial activity against human bacterial pathogen Escherichia coli (E. coli) showed that the inhibition zone produced by “biosynthesis” and “chemical reduction” Ag NPs were 3.17 cm and 2.42 cm respectively. With nanoparticles size smaller than 2 mm, antibacterial activity of “biosynthesis” Ag NPs against E. coli was 31 % higher than “chemical reduction” Ag NPs, although the concentration of Ag NPs produced by biosynthesis was about 10-fold less.

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Antibacterial activity of royal jelly-mediated green synthesized silver nanoparticles

AMB Express ◽

10.1186/s13568-021-01213-9 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Susanna Gevorgyan ◽

Robin Schubert ◽

Mkrtich Yeranosyan ◽

Lilit Gabrielyan ◽

Armen Trchounian ◽

...

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Green Synthesis ◽

Royal Jelly ◽

Fluorescence Emission ◽

Spherical Particles ◽

Ag Nps ◽

Gram Negative ◽

Green Synthesized Silver Nanoparticles ◽

Higher Sensitivity

AbstractThe application of green synthesis in nanotechnology is growing day by day. It’s a safe and eco-friendly alternative to conventional methods. The current research aimed to study raw royal jelly’s potential in the green synthesis of silver nanoparticles and their antibacterial activity. Royal jelly served as a reducing and oxidizing agent in the green synthesis technology of colloidal silver nanoparticles. The UV–Vis maximum absorption at ~ 430 nm and fluorescence emission peaks at ~ 487 nm confirmed the presence of Ag NPs. Morphology and structural properties of Ag NPs and the effect of ultrasound studies revealed: (i) the formation of polydispersed and spherical particles with different sizes; (ii) size reduction and homogeneity increase by ultrasound treatment. Antibacterial activity of different concentrations of green synthesized Ag NPs has been assessed on Gram-negative S. typhimurium and Gram-positive S. aureus, revealing higher sensitivity on Gram-negative bacteria.

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Green Synthesized Silver Nanoparticles Immobilized on Activated Carbon Nanoparticles: Antibacterial Activity Enhancement Study and Its Application on Textiles Fabrics

Molecules ◽

10.3390/molecules26133790 ◽

2021 ◽

Vol 26 (13) ◽

pp. 3790

Author(s):

Pratama Jujur Wibawa ◽

Muhammad Nur ◽

Mukhammad Asy’ari ◽

Wijanarka Wijanarka ◽

Heru Susanto ◽

...

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Activated Carbon ◽

Aloe Vera ◽

Particle Size Analysis ◽

Size Analysis ◽

E Coli ◽

Transmission Electron ◽

Average Size ◽

Green Synthesized Silver Nanoparticles

This research aimed to enhance the antibacterial activity of silver nanoparticles (AgNPs) synthesized from silver nitrate (AgNO3) using aloe vera extract. It was performed by means of incorporating AgNPs on an activated carbon nanoparticle (ACNPs) under ultrasonic agitation (40 kHz, 2 × 50 watt) for 30 min in an aqueous colloidal medium. The successful AgNPs synthesis was clarified with both Ultraviolet-Visible (UV-Vis) and Fourier Transform Infrared (FTIR) spectrophotometers. The successful AgNPs–ACNPs incorporation and its particle size analysis was performed using Transmission Electron Microscope (TEM). The brown color suspension generation and UV-Vis’s spectra maximum wavelength at around 480 nm confirmed the existence of AgNPs. The particle sizes of the produced AgNPs were about 5 to 10 nm in the majority number, which collectively surrounded the aloe vera extract secondary metabolites formed core-shell like nanostructure of 8.20 ± 2.05 nm in average size, while ACNPs themselves were about 20.10 ± 1.52 nm in average size formed particles cluster, and 48.00 ± 8.37 nm in average size as stacking of other particles. The antibacterial activity of the synthesized AgNPs and AgNPs-immobilized ACNPs was 57.58% and 63.64%, respectively (for E. coli); 61.25%, and 93.49%, respectively (for S. aureus). In addition, when the AgNPs-immobilized ACNPs material was coated on the cotton and polyester fabrics, the antibacterial activity of the materials changed, becoming 19.23% (cotton; E. coli), 31.73% (polyester; E. coli), 13.36% (cotton; S. aureus), 21.15% (polyester; S. aureus).

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Interior vs. Exterior Incorporation of Silver Nanoparticles in Cotton Fiber and Washing Durability

AATCC Journal of Research ◽

10.14504/ajr.8.6.1 ◽

2021 ◽

Vol 8 (6) ◽

pp. 1-12

Author(s):

Sunghyun Nam ◽

Sonia E. Chavez ◽

Matthew B. Hillyer ◽

Brian D. Condon ◽

Hongqing Shen ◽

...

Keyword(s):

Silver Nanoparticles ◽

Cotton Fabric ◽

Cotton Fiber ◽

Detergent Solution ◽

Ag Nps ◽

Antibacterial Performance ◽

The Poor ◽

Washing Durability ◽

Textile Products

The popular use of silver nanoparticles (Ag NPs) in commercial textile products that inhibit odor- and/or infection-causing bacteria has continuously raised concerns about their washing durability. The poor durability not only deteriorates the antibacterial performance, but also results in unwanted leaching of NPs into washing solutions. In this study, we showed how the incorporation location of Ag NPs—interior vs. exterior of cotton fiber—governs their durability against consecutive simulated home launderings. The superior washing durability of interior NPs was confirmed. The Ag losses after 50 laundering cycles for interior and exterior systems were 16% and 63% in water and 24% and 78% in detergent solution, respectively. The cotton fabric containing interior NPs predominantly released ionic Ag, whereas the fabric containing exterior NPs released particulate Ag.

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Synthesis of zinc oxide and silver nanoparticles using ficus palmata - Forssk leaf extracts and assessment of antibacterial activity

Environmental Engineering Research ◽

10.4491/eer.2020.454 ◽

2020 ◽

Vol 26 (6) ◽

pp. 200454-0

Author(s):

Sabaoon Shamshad ◽

Jamshaid Rashid ◽

Ihsan-ul-haq ◽

Naseem Iqbal ◽

Saif Ullah Awan

Keyword(s):

Staphylococcus Aureus ◽

Silver Nanoparticles ◽

Zinc Oxide ◽

Antibacterial Activity ◽

Zinc Oxide Nanoparticles ◽

Inhibitory Concentration ◽

Oxide Nanoparticles ◽

Leaf Extracts ◽

E Coli ◽

Ficus Palmata

Multidrug resistance of bacteria is an emerging human health hazard and warrants development of novel antibacterial agents with more effective mode of action. Here, zinc oxide and silver nanomaterials were prepared using Ficus palmata Forssk leaf extract with efficient antibacterial activity. SEM coupled with EDS confirmed the spherical symmetry with average particle diameter 50 to 65 nm while the XRD confirmed crystalline face centered cubic structure of silver and hexagonal crystallize phase of zinc oxide nanoparticles. Antibacterial activity was evaluated for 8 pathogenic bacterial strains including 3 drug resistant pathogenic strains. The nanoparticles showed enhanced growth inhibition for resistant strains in comparison with the broad-spectrum antibiotics i.e. roxithromycin and cefixime. Minimum inhibitory concentration in μg.mL-1 of silver nanoparticles was found to be as low as 33.3 for resistant Streptococcus haemolyticus; 11.1 for Staphylococcus aureus and E Coli; and 3.7 μg.mL-1 for resistant Pseudomonas aeruginosa. Similarly, the minimum inhibitory concentration of zinc oxide nanoparticles was found to be 100 μg.mL-1 against resistant Streptococcus haemolyticus and Staphylococcus aureus; 11.1 μg.mL-1 for resistant Pseudomonas aeruginosa; and 3.7 μg.mL-1 against resistant E coli. Ficus palmata Forssk leaf extracts can be explored effectively for synthesizing active antibacterial nanomaterials as a non-toxic and environmentally benign synthesis route.

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