Reactive group effects on the photophysical and biological properties of 2-phenyl-1H-phenanthro[9,10-d]imidazole derivatives as fluorescent markers

2018 ◽  
Vol 16 (20) ◽  
pp. 3788-3800 ◽  
Author(s):  
Przemysław Krawczyk ◽  
Przemysław Czeleń ◽  
Piotr Cysewski
Keyword(s):  
Medical Imaging ◽  
Fluorescent Probes ◽  
Biomedical Imaging ◽  
Biological Properties ◽  
Reactive Group ◽  
Disulfide Group ◽  
Imidazole Derivatives ◽  
Fluorescent Markers ◽  
Group Effects

The presence of a pyridyl disulfide group limits the use of phenanthroimidazole derivatives in medical imaging, while the introduction of an N-hydroxysuccinimide ester along with changes to the PhI backbone may be used to create valuable fluorescent probes for applications in biomedical imaging.

scholarly journals Continuous-Wave THz Imaging for Biomedical Samples

2020 ◽  
Vol 11 (1) ◽  
pp. 71
Author(s):  
Yaya Zhang ◽  
Chuting Wang ◽  
Bingxin Huai ◽  
Shiyu Wang ◽  
Yating Zhang ◽  
...  
Keyword(s):  
Medical Imaging ◽  
Biomedical Imaging ◽  
Continuous Wave ◽  
Biological Tissues ◽  
Thz Spectroscopy ◽  
Label Free ◽  
Thz Imaging ◽  
Malignant Tumours ◽  
Advantages And Disadvantages ◽  
Imaging Application

In the past few decades, the applications of terahertz (THz) spectroscopy and imaging technology have seen significant developments in the fields of biology, medical diagnosis, food safety, and nondestructive testing. Label-free diagnosis of malignant tumours has been obtained and also achieved significant development in THz biomedical imaging. This review mainly presents the research status and prospects of several common continuous-wave (CW) THz medical imaging systems and applications of THz medical imaging in biological tissues. Here, we first introduce the properties of THz waves and how these properties play a role in biomedical imaging. Then, we analyse both the advantages and disadvantages of the CW THz imaging methods and the progress of these methods in THz biomedical imaging in recent ten years. Finally, we summarise the obstacles in the way of the application of THz bio-imaging application technology in clinical detection, which need to be investigated and overcome in the future.

Intelligent Computing in Medical Imaging

2021 ◽  
pp. 592-608
Author(s):  
Shouvik Chakraborty ◽  
Sankhadeep Chatterjee ◽  
Amira S. Ashour ◽  
Kalyani Mali ◽  
Nilanjan Dey
Keyword(s):  
Image Analysis ◽  
Medical Imaging ◽  
Biomedical Imaging ◽  
Clinical Analysis ◽  
Biological Species ◽  
Imaging Modalities ◽  
Biomedical Image Analysis ◽  
Accurate Analysis ◽  
Intelligent Computing ◽  
Biomedical Image

Biomedical imaging is considered main procedure to acquire valuable physical information about the human body and some other biological species. It produces specialized images of different parts of the biological species for clinical analysis. It assimilates various specialized domains including nuclear medicine, radiological imaging, Positron emission tomography (PET), and microscopy. From the early discovery of X-rays, progress in biomedical imaging continued resulting in highly sophisticated medical imaging modalities, such as magnetic resonance imaging (MRI), ultrasound, Computed Tomography (CT), and lungs monitoring. These biomedical imaging techniques assist physicians for faster and accurate analysis and treatment. The present chapter discussed the impact of intelligent computing methods for biomedical image analysis and healthcare. Different Artificial Intelligence (AI) based automated biomedical image analysis are considered. Different approaches are discussed including the AI ability to resolve various medical imaging problems. It also introduced the popular AI procedures that employed to solve some special problems in medicine. Artificial Neural Network (ANN) and support vector machine (SVM) are active to classify different types of images from various imaging modalities. Different diagnostic analysis, such as mammogram analysis, MRI brain image analysis, CT images, PET images, and bone/retinal analysis using ANN, feed-forward back propagation ANN, probabilistic ANN, and extreme learning machine continuously. Various optimization techniques of ant colony optimization (ACO), genetic algorithm (GA), particle swarm optimization (PSO) and other bio-inspired procedures are also frequently conducted for feature extraction/selection and classification. The advantages and disadvantages of some AI approaches are discussed in the present chapter along with some suggested future research perspectives.

THE BIOLOGICAL PROPERTIES OF 3,6-EPIDITHIADIKETO-PIPERAZINES. DEGRADATION OF GLIOTOXIN-B BY BACILLUS SUBTIL IS (HLX 373)

1967 ◽  
Vol 13 (12) ◽  
pp. 1577-1589 ◽  
Author(s):  
D. Brewer ◽  
A. Taylor
Keyword(s):  
Bacillus Subtilis ◽  
Biological Properties ◽  
Chemical Degradation ◽  
Available Nitrogen ◽  
Disulfide Group ◽  
Number Of Cells

Like sporidesmin, gliotoxin catalyzes the decomposition of azide in the presence of iodine, and this catalysis can be used for its estimation. The presence of respiring cells of Bacillus subtilis does not interfere with the estimation. Thus it was possible to show that the concentration of gliotoxin decreased when solutions of the antibiotic were incubated with respiring cells. The rate of the decomposition was independent of the gliotoxin concentration in the range 2–25 μg/ml, but was dependent on the number of cells, the pH, and the available nitrogen in the suspending medium. Loss of gliotoxin was most rapid at pH 5.5 and continued when the supernatant was reincubated after centrifugal removal of the cells. No loss of gliotoxin occurred when solutions were incubated in the absence of cells at pH 5.5. By contrast, chemical degradation at pH 8.0 was inhibited in the presence of cells. These results are consistent with the hypothesis that B. subtilis secretes an enzyme which degrades the disulfide group in gliotoxin.

scholarly journals A Review of Tools Applied in Processing of Medical Images

2021 ◽  
pp. 42-49
Author(s):  
Anandakumar Haldorai ◽  
Shrinand Anandakumar
Keyword(s):  
Medical Imaging ◽  
Operating Systems ◽  
Medical Image ◽  
Biomedical Imaging ◽  
Software Tool ◽  
Detailed Examination ◽  
Speed Up ◽  
Different Levels ◽  
Therapy Treatment ◽  
Analyze Data

The segmentation step of therapy treatment includes a detailed examination of medical imaging. In diagnosis, clinical research, and patient management, medical pictures are mainly utilized as radiographic methods. Image processing software for medical imaging is also crucial. It is possible to improve and speed up the analysis of a medical picture using a bioMIP technique. This article presents a biomedical imaging software tool that aims to provide a similar level of programmability while investigating pipelined processor solutions. These tools mimic entire systems made up of many of the recommended processing segment within the setups categorized by the schematic framework. In this paper, 15 biomedical imaging technologies will be evaluated on a number of different levels. The comparison's primary goal is to collect and analyze data in order to suggest which medical image program should be used when analyzing various kinds of imaging to users of various operating systems. The article included a result table that was reviewed.

scholarly journals Highly Fluorescent Distyrylnaphthalene Derivatives as a Tool for Visualization of Cellular Membranes

Materials ◽  
2020 ◽  
Vol 13 (4) ◽  
pp. 951
Author(s):  
Justyna Suwara ◽  
Beata Lukasik ◽  
Remigiusz Zurawinski ◽  
Roza Pawlowska ◽  
Arkadiusz Chworos
Keyword(s):  
Fluorescent Probes ◽  
Cell Biology ◽  
Biological Properties ◽  
Fluorescent Imaging ◽  
Staining Procedure ◽  
Complete Medium ◽  
Cellular Membranes ◽  
Suspension Cells ◽  
Cellular Processes ◽  
Wide Range

Fluorescent imaging, which is an important interdisciplinary field bridging research from organic chemistry, biochemistry and cell biology has been applied for multi-dimensional detection, visualization and characterization of biological structures and processes. Especially valuable is the possibility to monitor cellular processes in real time using fluorescent probes. In this work, conjugated oligoelectrolytes and neutral derivatives with the distyrylnaphthalene core (SN-COEs) were designed, synthetized and tested for biological properties as membrane-specific fluorescent dyes for the visualization of membrane-dependent cellular processes. The group of tested compounds includes newly synthesized distyrylnaphthalene derivatives (DSNNs): a trimethylammonium derivative (DSNN-NMe3+), a phosphonate derivative (DSNN-P), a morpholine derivative (DSNN-Mor), a dihydroxyethylamine derivative (DSNN-DEA), a phosphonate potassium salt (DSNN-POK), an amino derivative (DSNN-NH2) and pyridinium derivative (DSNN-Py+). All compounds were tested for their biological properties, including cytotoxicity and staining efficiency towards mammalian cells. The fluorescence intensity of SN-COEs incorporated into cellular structures was analyzed by fluorescence activated cell sorting (FACS) and photoluminescence spectroscopy. The cytotoxicity results have shown that all tested SN-COEs can be safely used in the human and animal cell studies. Fluorescence and confocal microscopy observations confirm that tested COEs can be applied as fluorescent probes for the visualization of intracellular membrane components in a wide range of different cell types, including adherent and suspension cells. The staining procedure may be performed under both serum free and complete medium conditions. The presented studies have revealed the interesting biological properties of SN-COEs and confirmed their applicability as dyes for staining the membranous structures of eukaryotic cells, which may be useful for visualization of wide range of biological processes dependent of the extra-/intracellular communications and/or based on the remodeling of cellular membranes.

Current Advances in the Synthesis and Biological Evaluation of Pharmacologically Relevant 1,2,4,5-Tetrasubstituted-1H-Imidazole Derivatives

2019 ◽  
Vol 23 (19) ◽  
pp. 2016-2101 ◽  
Author(s):  
Renzo Rossi ◽  
Maurizio Ciofalo
Keyword(s):  
Molecular Targets ◽  
Research Area ◽  
Biological Properties ◽  
Biological Evaluation ◽  
Imidazole Derivatives ◽  
Recent Developments ◽  
The Subject ◽  
Valuable Compounds ◽  
Synthesis And Biological Evaluation ◽  
Potential Use

: In recent years, the synthesis and evaluation of the biological properties of 1,2,4,5-tetrasubstituted-1H-imidazole derivatives have been the subject of a large number of studies by academia and industry. In these studies it has been shown that this large and highly differentiated class of heteroarene derivatives includes high valuable compounds having important biological and pharmacological properties such as antibacterial, antifungal, anthelmintic, anti-inflammatory, anticancer, antiviral, antihypertensive, cholesterol-lowering, antifibrotic, antiuricemic, antidiabetic, antileishmanial and antiulcer activities. : The present review with 411 references, in which we focused on the literature data published mainly from 2011 to 2017, aims to update the readers on the recent developments on the synthesis and biological evaluation of pharmacologically relevant 1,2,4,5-tetrasubstituted-1H-imidazole derivatives with an emphasis on their different molecular targets and their potential use as drugs to treat various types of diseases. Reference was also made to substantial literature data acquired before 2011 in this burgeoning research area.

scholarly journals Effect of the Chloro-Substitution on Electrochemical and Optical Properties of New Carbazole Dyes

Materials ◽  
2021 ◽  
Vol 14 (11) ◽  
pp. 3091
Author(s):  
Przemysław Krawczyk ◽  
Beata Jędrzejewska ◽  
Klaudia Seklecka ◽  
Joanna Cytarska ◽  
Krzysztof Z. Łączkowski
Keyword(s):  
Optical Properties ◽  
Fluorescent Probes ◽  
Quantum Chemical ◽  
Biological Properties ◽  
Biologically Active ◽  
In Vitro Tests ◽  
Naturally Occurring ◽  
Active Substances

Carbazole derivatives are the structural key of many biologically active substances, including naturally occurring and synthetic ones. Three novel (E)-2-(2-(4-9H-carbazol-9-yl)benzylidene)hydrazinyl)triazole dyes were synthesized with different numbers of chlorine substituents attached at different locations. The presented research has shown the influence of the number and position of attachment of chlorine substituents on electrochemical, optical, nonlinear, and biological properties. The study also included the analysis of the use of the presented derivatives as potential fluorescent probes for in vivo and in vitro tests. Quantum-chemical calculations complement the conducted experiments.

scholarly journals Robust and Bright Genetically Encoded Fluorescent Markers for Highlighting Structures and Compartments in Mammalian Cells

2017 ◽  
Author(s):  
Anna O Chertkova ◽  
Marieke Mastop ◽  
Marten Postma ◽  
Nikki van Bommel ◽  
Sanne van der Niet ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Mammalian Cells ◽  
Protein Domains ◽  
Spectroscopic Properties ◽  
Living Cells ◽  
Cellular Structures ◽  
Signal Sequences ◽  
Fluorescent Marker ◽  
Fluorescent Markers

To increase our understanding of the inner working of cells, there is a need for specific markers to identify biomolecules, cellular structures and compartments. One type of markers comprises genetically encoded fluorescent probes that are linked with protein domains, peptides and/or signal sequences. These markers are encoded on a plasmid and they allow straightforward, convenient labeling of cultured mammalian cells by introducing the plasmid into the cells. Ideally, the fluorescent marker combines favorable spectroscopic properties (brightness, photostability) with specific labeling of the structure or compartment of interest. Here, we report our ongoing efforts to generate robust and bright genetically encoded fluorescent markers for highlighting structures and compartments in living cells. The plasmids are distributed by addgene: https://www.addgene.org/browse/article/28189953/

scholarly journals Triazole-imidazole (TA-IM) derivatives as ultrafast fluorescent probes for selective Ag+ detection

2018 ◽  
Vol 16 (42) ◽  
pp. 7801-7805 ◽  
Author(s):  
Qi Lai ◽  
Qing Liu ◽  
Ying He ◽  
Kai Zhao ◽  
Chiyu Wei ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Silver Ion ◽  
Ion Detection ◽  
Imidazole Derivatives

1,2,3-Triazole-imidazole derivatives (TA-IM) were prepared as fluorescent probes for silver ion detection.

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