Analysis of three-dimensional biofilms on different material surfaces

2020 ◽  
Vol 8 (12) ◽  
pp. 3500-3510
Author(s):  
Juliane Schiebel ◽  
Jonas Noack ◽  
Stefan Rödiger ◽  
Anne Kammel ◽  
Friederike Menzel ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Three Dimensional ◽  
Epifluorescence Microscopy ◽  
Analysis Software ◽  
Material Surfaces

We developed a combination of an automated epifluorescence microscopy system with the analysis software Open-Biofilm-Altimeter for semi-quantitative analysis of three-dimensional biofilms.

Study on erythrocytes by SEM photogrammetry (SEMP) technique

1990 ◽  
Vol 48 (3) ◽  
pp. 724-725
Author(s):  
Tong Wensheng ◽  
Lu Lianhuang ◽  
Zhang Zhijun
Keyword(s):  
Quantitative Analysis ◽  
Cell Membrane ◽  
Clinical Diagnosis ◽  
Human Body ◽  
Blood Cells ◽  
Three Dimensional ◽  
Normal Person ◽  
Blood Disease ◽  
Computation Technique ◽  
Important Basis

This is a combined study of two diffirent branches, photogrammetry and morphology of blood cells. The three dimensional quantitative analysis of erythrocytes using SEMP technique, electron computation technique and photogrammetry theory has made it possible to push the study of mophology of blood cells from LM, TEM, SEM to a higher stage, that of SEM P. A new path has been broken for deeply study of morphology of blood cells.In medical view, the abnormality of the quality and quantity of erythrocytes is one of the important changes of blood disease. It shows the abnormal blood—making function of the human body. Therefore, the study of the change of shape on erythrocytes is the indispensable and important basis of reference in the clinical diagnosis and research of blood disease.The erythrocytes of one normal person, three PNH Patients and one AA patient were used in this experiment. This research determines the following items: Height;Length of two axes (long and short), ratio; Crevice in depth and width of cell membrane; Circumference of erythrocytes; Isoline map of erythrocytes; Section map of erythrocytes.

scholarly journals Maneuverability of the Scope and Instruments within Three Different Single-Incision Laparoscopic Ports: An Experimental Pilot Study

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1242
Author(s):  
Georg Haider ◽  
Ursula Schulz ◽  
Nikola Katic ◽  
Christian Peham ◽  
Gilles Dupré
Keyword(s):  
Motion Analysis ◽  
Single Incision ◽  
Single Port ◽  
Three Dimensional ◽  
Analysis Software ◽  
Port Access ◽  
Port System ◽  
Sils Port ◽  
Glove Port ◽  
Analysis System

Single-port access systems (SPASs) are currently used in human and veterinary surgeries. However, they pose technical challenges, such as instrument crowding, intra- and extracorporeal instrument collision, and reduced maneuverability. Studies comparing the maneuverability of the scopes and instruments in different SPASs are lacking. This study aimed to compare the maneuverability of three different SPASs: the Covidien SILS-port, Storz Endocone, and glove port. A clear acrylic box with artificial skin placed at the bottom was used to mimic the abdominal wall and cavity. The three SPASs were placed from below, and a 10-mm endoscope and 5-mm instrument were introduced. A motion analysis system consisting of 18 cameras and motion analysis software were used to track the movement of the endoscope and instrument, to determine the volume of the cone-shaped, three-dimensional figures over which movement was possible, with higher values indicating greater maneuverability. The Mann–Whitney U test was used for the analysis. The maneuverability of the endoscope alone was significantly higher in the glove port system than in the other two SPASs. When inserting an additional instrument, the maneuverability significantly decreased in the SILS-port and Endocone, but not in the glove port. The highest maneuverability overall was found in the glove port.

scholarly journals A quantitative analysis software tool for mass spectrometry–based proteomics

2008 ◽  
Vol 5 (4) ◽  
pp. 319-322 ◽  
Author(s):  
Sung Kyu Park ◽  
John D Venable ◽  
Tao Xu ◽  
John R Yates
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Analysis ◽  
Software Tool ◽  
Analysis Software

Sight distance red zones on combined horizontal and sag vertical curves

1998 ◽  
Vol 25 (4) ◽  
pp. 621-630 ◽  
Author(s):  
Yasser Hassan ◽  
Said M Easa
Keyword(s):  
Quantitative Analysis ◽  
Three Dimensional ◽  
The Other ◽  
Two Dimensional ◽  
Horizontal Curve ◽  
Sight Distance ◽  
Stopping Sight Distance ◽  
Three Dimensional Models ◽  
Highway Alignment ◽  
Current Standards

Coordination of highway horizontal and vertical alignments is based on subjective guidelines in current standards. This paper presents a quantitative analysis of coordinating horizontal and sag vertical curves that are designed using two-dimensional standards. The locations where a horizontal curve should not be positioned relative to a sag vertical curve (called red zones) are identified. In the red zone, the available sight distance (computed using three-dimensional models) is less than the required sight distance. Two types of red zones, based on stopping sight distance (SSD) and preview sight distance (PVSD), are examined. The SSD red zone corresponds to the locations where an overlap between a horizontal curve and a sag vertical curve should be avoided because the three-dimensional sight distance will be less than the required SSD. The PVSD red zone corresponds to the locations where a horizontal curve should not start because drivers will not be able to perceive it and safely react to it. The SSD red zones exist for practical highway alignment parameters, and therefore designers should check the alignments for potential SSD red zones. The range of SSD red zones was found to depend on the different alignment parameters, especially the superelevation rate. On the other hand, the results showed that the PVSD red zones exist only for large values of the required PVSD, and therefore this type of red zones is not critical. This paper should be of particular interest to the highway designers and professionals concerned with highway safety.Key words: sight distance, red zone, combined alignment.

scholarly journals Quantitative analysis of normal fetal brain volume and flow by three-dimensional power Doppler ultrasound

2013 ◽  
Vol 76 (9) ◽  
pp. 504-509 ◽  
Author(s):  
Ju-Chun Hsu ◽  
Yi-Cheng Wu ◽  
Peng-Hui Wang ◽  
Hsing-I Wang ◽  
Chi-Mou Juang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Doppler Ultrasound ◽  
Brain Volume ◽  
Power Doppler ◽  
Three Dimensional ◽  
Fetal Brain ◽  
Power Doppler Ultrasound

Quantitative analysis of the three dimensional fetoplacental vascular tree in normal, term placenta

Placenta ◽  
2017 ◽  
Vol 57 ◽  
pp. 239-240 ◽  
Author(s):  
Andrew Melbourne ◽  
Rosalind Pratt ◽  
Ciaran Hutchinson ◽  
Owen Arthurs ◽  
Neil J. Sebire ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Three Dimensional ◽  
Vascular Tree ◽  
Term Placenta ◽  
Normal Term

scholarly journals A microscopic approach to investigate bacteria under in situ conditions in sea-ice samples

2001 ◽  
Vol 33 ◽  
pp. 304-310 ◽  
Author(s):  
Karen Junge ◽  
Christopher Krembs ◽  
Jody Deming ◽  
Aaron Stierle ◽  
Hajo Eicken
Keyword(s):  
Sea Ice ◽  
Pore Space ◽  
Three Dimensional ◽  
Epifluorescence Microscopy ◽  
Microbial Populations ◽  
Microbial Life ◽  
Dimensional Network ◽  
In Situ Conditions ◽  
Fluorescent Stain

AbstractMicrobial populations and activity within sea ice have been well described based on bulk measurements from melted sea-ice samples. However, melting destroys the micro-environments within the ice matrix and does not allow for examination of microbial populations at a spatial scale relevant to the organism. Here, we describe the development of a new method allowing for microscopic observations of bacteria localized within the three-dimensional network of brine inclusions in sea ice under in situ conditions. Conventional bacterial staining procedures, using the DNA-specific fluorescent stain DAPI, epifluorescence microscopy and image analysis, were adapted to examine bacteria and their associations with various surfaces within microtomed sections of sea ice at temperatures from −2° to −15°C. The utility and sensitivity of the method were demonstrated by analyzing artificial sea-ice preparations of decimal dilutions of a known bacterial culture. When applied to natural, particle-rich sea ice, the method allowed distinction between bacteria and particles at high magnification. At lower magnifications, observations of bacteria could be combined with those of other organisms and with morphology and particle content of the pore space. The method described here may ultimately aid in discerning constraints on microbial life at extremely low temperatures.

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