Selection of threose nucleic acid aptamers to block PD-1/PD-L1 interaction for cancer immunotherapy

2020 ◽  
Vol 56 (93) ◽  
pp. 14653-14656
Author(s):  
Xintong Li ◽  
Zhe Li ◽  
Hanyang Yu
Keyword(s):  
Nucleic Acid ◽  
Mouse Model ◽  
Cancer Immunotherapy ◽  
Tumour Growth ◽  
Xenograft Mouse Model ◽  
Nucleic Acid Aptamers ◽  
Threose Nucleic Acid ◽  
Selection Of

In vitro selected threose nucleic acid (TNA) aptamers blocked PD-1/PD-L1 interaction and significantly inhibited tumour growth in xenograft mouse model.

Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography

2007 ◽  
Vol 390 (4) ◽  
pp. 1033-1037 ◽  
Author(s):  
Jens Müller ◽  
Osman El-Maarri ◽  
Johannes Oldenburg ◽  
Bernd Pötzsch ◽  
Günter Mayer
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Nucleic Acid ◽  
High Performance ◽  
In Vitro Selection ◽  
Nucleic Acid Aptamers ◽  
Selection Of

[20] In vitro selection of nucleic acid aptamers that bind proteins

1996 ◽  
pp. 336-367 ◽  
Author(s):  
Richard C. Conrad ◽  
Lori Giver ◽  
Yu Tian ◽  
Andrew D. Ellington
Keyword(s):  
Nucleic Acid ◽  
In Vitro Selection ◽  
Nucleic Acid Aptamers ◽  
Selection Of

scholarly journals A novel phosphoramide compound, DCZ0805, shows potent anti-myeloma activity via the NF-κB pathway

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Xuejie Gao ◽  
Bo Li ◽  
Anqi Ye ◽  
Houcai Wang ◽  
Yongsheng Xie ◽  
...  
Keyword(s):  
Mouse Model ◽  
Tumor Burden ◽  
High Rate ◽  
Cell Counting ◽  
Tumor Xenograft ◽  
Luciferase Reporter ◽  
Therapeutic Effects ◽  
Xenograft Mouse Model

Abstract Background Multiple myeloma (MM) is a highly aggressive and incurable clonal plasma cell disease with a high rate of recurrence. Thus, the development of new therapies is urgently needed. DCZ0805, a novel compound synthesized from osalmide and pterostilbene, has few observed side effects. In the current study, we intend to investigate the therapeutic effects of DCZ0805 in MM cells and elucidate the molecular mechanism underlying its anti-myeloma activity. Methods We used the Cell Counting Kit-8 assay, immunofluorescence staining, cell cycle assessment, apoptosis assay, western blot analysis, dual-luciferase reporter assay and a tumor xenograft mouse model to investigate the effect of DCZ0805 treatment both in vivo and in vitro. Results The results showed that DCZ0805 treatment arrested the cell at the G0/G1 phase and suppressed MM cells survival by inducing apoptosis via extrinsic and intrinsic pathways. DCZ0805 suppressed the NF-κB signaling pathway activation, which may have contributed to the inhibition of cell proliferation. DCZ0805 treatment remarkably reduced the tumor burden in the immunocompromised xenograft mouse model, with no obvious toxicity observed. Conclusion The findings of this study indicate that DCZ0805 can serve as a novel therapeutic agent for the treatment of MM.

scholarly journals Hybrid-Type SELEX for the Selection of Artificial Nucleic Acid Aptamers Exhibiting Cell Internalization Activity

Pharmaceutics ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 888
Author(s):  
Hiro Uemachi ◽  
Yuuya Kasahara ◽  
Keisuke Tanaka ◽  
Takumi Okuda ◽  
Yoshihiro Yoneda ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Surface Protein ◽  
Functional Screening ◽  
Nucleic Acid Aptamers ◽  
Hybrid Type ◽  
Cell Internalization ◽  
Promising Target ◽  
A Cell ◽  
Exponential Enrichment

Nucleic acid aptamers have attracted considerable attention as next-generation pharmaceutical agents and delivery vehicles for small molecule drugs and therapeutic oligonucleotides. Chemical modification is an effective approach for improving the functionality of aptamers. However, the process of selecting appropriately modified aptamers is laborious because of many possible modification patterns. Here, we describe a hybrid-type systematic evolution of ligands by exponential enrichment (SELEX) approach for the generation of the artificial nucleic acid aptamers effective against human TROP2, a cell surface protein identified by drug discovery as a promising target for cancer therapy. Capillary electrophoresis SELEX was used for the pre-screening of multiple modified nucleic acid libraries and enrichment of TROP2 binding aptamers in the first step, followed by functional screening using cell-SELEX in the second step for the generation of cell-internalizing aptamers. One representative aptamer, Tac-B1, had a nanomolar-level affinity to human TROP2 and exhibited elevated capacity for internalization by cells. Because of the growing interest in the application of aptamers for drug delivery, our hybrid selection approach has great potential for the generation of functional artificial nucleic acid aptamers with ideal modification patterns in vitro.

Anticancer effect of arsenic trioxide on cholangiocarcinoma: in vitro experiments and in vivo xenograft mouse model

2013 ◽  
Vol 14 (2) ◽  
pp. 215-224 ◽  
Author(s):  
Eun-Young Kim ◽  
Sang Soo Lee ◽  
Ji Hoon Shin ◽  
Soo Hyun Kim ◽  
Dong-Ho Shin ◽  
...  
Keyword(s):  
Mouse Model ◽  
Arsenic Trioxide ◽  
In Vitro Experiments ◽  
Anticancer Effect ◽  
Xenograft Mouse Model

Dimethyl fumarate and vitamin D derivatives cooperatively enhance VDR and Nrf2 signaling in differentiating AML cells in vitro and inhibit leukemia progression in a xenograft mouse model

2019 ◽  
Vol 188 ◽  
pp. 8-16 ◽  
Author(s):  
Matan Nachliely ◽  
Aviram Trachtenberg ◽  
Boris Khalfin ◽  
Karen Nalbandyan ◽  
Merav Cohen-Lahav ◽  
...  
Keyword(s):  
Vitamin D ◽  
Mouse Model ◽  
Dimethyl Fumarate ◽  
Xenograft Mouse Model

scholarly journals Functional Gene Knockout of NRF2 Increases Chemosensitivity of Human Lung Cancer A549 Cells In Vitro and in a Xenograft Mouse Model

2018 ◽  
Vol 11 ◽  
pp. 75-89 ◽  
Author(s):  
Pawel Bialk ◽  
Yichen Wang ◽  
Kelly Banas ◽  
Eric B. Kmiec
Keyword(s):  
Lung Cancer ◽  
Mouse Model ◽  
Human Lung ◽  
Gene Knockout ◽  
A549 Cells ◽  
Functional Gene ◽  
Human Lung Cancer ◽  
Xenograft Mouse Model

scholarly journals Adenovirus-Mediated Expression of the p14 Fusion-Associated Small Transmembrane Protein Promotes Cancer Cell Fusion and Apoptosis In Vitro but Does Not Provide Therapeutic Efficacy in a Xenograft Mouse Model of Cancer

PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0151516 ◽  
Author(s):  
Carmen M. Wong ◽  
Kathy L. Poulin ◽  
Grace Tong ◽  
Carin Christou ◽  
Michael A. Kennedy ◽  
...  
Keyword(s):  
Mouse Model ◽  
Cell Fusion ◽  
Cancer Cell ◽  
Therapeutic Efficacy ◽  
Transmembrane Protein ◽  
Xenograft Mouse Model

scholarly journals Dihydroisotanshinone I as a Treatment Option for Head and Neck Squamous Cell Carcinomas

2021 ◽  
Vol 22 (16) ◽  
pp. 8881
Author(s):  
Cheng-Ming Hsu ◽  
Ming-Yu Yang ◽  
Ming-Shao Tsai ◽  
Geng-He Chang ◽  
Yao-Hsu Yang ◽  
...  
Keyword(s):  
Adverse Effects ◽  
Mouse Model ◽  
Head And Neck ◽  
Squamous Cell ◽  
Salvia Miltiorrhiza ◽  
Chinese Herb ◽  
Squamous Cell Carcinomas ◽  
Xenograft Mouse Model ◽  
Chemotherapy Drugs

Head and neck squamous cell carcinomas (HNSCCs) are the most common cancers of the head and neck, and their prevalence is rapidly increasing. HNSCCs present a clinical challenge because of their high recurrence rate, therapeutic resistance to radiation and chemotherapy drugs, and adverse effects. Hence, traditional Chinese herbal treatment may be advantageous to therapeutic strategies for HNSCCs. Danshen (Salvia miltiorrhiza), a well-known Chinese herb, has been extensively applied in treatments for various diseases, including cancer, because of its high degree of safety and low rate of adverse effects despite its unclear mechanism. Thus, we aimed to explore the possible anticancer effects and mechanisms of dihydroisotanshinone I (DT), a compound in danshen (extract from danshen), on HNSCCs. Three HNSCCs cell lines were used for in vitro studies, and a Detroit 562 xenograft mouse model was chosen for in vivo studies. Our in vitro results showed that DT could initiate apoptosis, resulting in cell death, and the p38 signaling partially regulated DT-initiated cell apoptosis in the Detroit 562 model. In the xenograft mouse model, DT reduced tumor size with no obvious adverse effect of hepatotoxicity. The present study suggests that DT is a promising novel candidate for anti-HNSCCs therapy.

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