Rapid detection of silver ions based on luminescent carbon nanodots for multicolor patterning, smartphone sensors, and bioimaging applications

2021 ◽  
Author(s):  
Sonaimuthu Mohandoss ◽  
Palanisamy Subramanian ◽  
SangGuan You ◽  
Jae-Jin Shim ◽  
Yong Rok Lee

Photoluminescent nitrogen and sulfur co-doped carbon nanodots (N,S-CNDs) are prepared via single-step hydrothermal carbonization using 2,4-diaminobenzenesulfonic acid (2,4-DABSA) as a sole precursor. The synthesized N,S-CNDs are easily dispersed in aqueous...

2020 ◽  
Vol 56 (8) ◽  
pp. 5041-5052
Author(s):  
Leqi Hu ◽  
Fuchao Jia ◽  
Shuo Wang ◽  
Xingyan Shao ◽  
Xiaomei Wang ◽  
...  

2021 ◽  
Author(s):  
Young Jun Seo ◽  
Tasnima Alam Asa ◽  
Ravi Kumara Guralamatta Siddappa

In this study we developed a very simple and rapid miRNA 21 detection system using a novel quinolinium diethylamino salicylaldehyde (QnDESA) probe for sensing the 22AG hybrid G-quadruplex with single-step...


2002 ◽  
Vol 81 (7) ◽  
pp. 472-476 ◽  
Author(s):  
F.R. Tay ◽  
D.H. Pashley ◽  
M. Yoshiyama

Self-etch adhesives that etch, prime, and bond simultaneously should not exhibit incomplete resin infiltration within hybrid layers. We hypothesized that nanoleakage patterns in these systems are artifacts caused by mineral dissolution in mildly acidic silver nitrate. Resin-dentin interfaces bonded with four single-step, self-etch adhesives were examined for nanoleakage by conventional (pH 4.2) and basic ammoniacal (pH 9.5) silver nitrate and prepared for transmission electron microscopy. All adhesives exhibited a reticular mode of nanoleakage within hybrid layers when conventional silver nitrate was used. With ammoniacal silver nitrate, an additional spotted pattern of nanoleakage was observed within adhesive and hybrid layers. The reticular mode of nanoleakage in self-etch adhesives probably represents sites of incomplete water removal that leads to regional suboptimal polymerization. The spotted pattern identified with the use of ammoniacal silver nitrate probably represents potentially permeable regions in the adhesive and hybrid layers that result from the interaction of the basic diamine silver ions with acidic/hydrophilic resin components.


Author(s):  
Yueying Wang ◽  
Xuetao Hu ◽  
Wenting Li ◽  
Xiaowei Huang ◽  
Zhihua Li ◽  
...  

2019 ◽  
Vol 146 ◽  
pp. 872-880 ◽  
Author(s):  
Waqas Ahmad ◽  
Huanhuan Li ◽  
Md Mehedi Hassan ◽  
Jingjing Wang ◽  
Muhammad Zareef ◽  
...  

2019 ◽  
Vol 5 (2) ◽  
pp. 24 ◽  
Author(s):  
Ganeshlenin Kandasamy

Carbon quantum dots (CQDs)/carbon nanodots are a new class of fluorescent carbon nanomaterials having an approximate size in the range of 2–10 nm. The majority of the reported review articles have discussed about the development of the CQDs (via simple and cost-effective synthesis methods) for use in bio-imaging and chemical-/biological-sensing applications. However, there is a severe lack of consolidated studies on the recently developed CQDs (especially doped/co-doped) that are utilized in different areas of application. Hence, in this review, we have extensively discussed about the recent development in doped and co-doped CQDs (using elements/heteroatoms—e.g., boron (B), fluorine (F), nitrogen (N), sulphur (S), and phosphorous (P)), along with their synthesis method, reaction conditions, and/or quantum yield (QY), and their emerging multi-potential applications including electrical/electronics (such as light emitting diode (LED) and solar cells), fluorescent ink for anti-counterfeiting, optical sensors (for detection of metal ions, drugs, and pesticides/fungicides), gene delivery, and temperature probing.


Author(s):  
Jean Y. H. Lee ◽  
Nickala Best ◽  
Julie McAuley ◽  
Jessica L. Porter ◽  
Torsten Seemann ◽  
...  

2.AbstractIntroductionThe SARS-CoV-2 pandemic of 2020 has resulted in unparalleled requirements for RNA extraction kits and enzymes required for virus detection, leading to global shortages. This has necessitated the exploration of alternative diagnostic options to alleviate supply chain issues.AimTo establish and validate a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of SARS-CoV-2 from nasopharyngeal swabs.MethodologyWe used a commercial RT-LAMP mastermix from OptiGene Ltd in combination with a primer set designed to detect the CDC N1 region of the SARS-CoV-2 nucleocapsid (N) gene. A single-tube, single-step fluorescence assay was implemented whereby as little as 1 μL of universal transport medium (UTM) directly from a nasopharyngeal swab could be used as template, bypassing the requirement for RNA purification. Amplification and detection could be conducted in any thermocycler capable of holding 65°C for 30 minutes and measure fluorescence in the FAM channel at one-minute intervals.ResultsAssay evaluation by assessment of 157 clinical specimens previously screened by E-gene RT-qPCR revealed assay sensitivity and specificity of 87% and 100%, respectively. Results were fast, with an average time-to-positive (Tp) for 93 clinical samples of 14 minutes (SD ±7 minutes). Using dilutions of SARS-CoV-2 virus spiked into UTM, we also evaluated assay performance against FDA guidelines for implementation of emergency-use diagnostics and established a limit-of-detection of 54 Tissue Culture Infectious Dose 50 per ml (TCID50 mL−1), with satisfactory assay sensitivity and specificity. A comparison of 20 clinical specimens between four laboratories showed excellent interlaboratory concordance; performing equally well on three different, commonly used thermocyclers, pointing to the robustness of the assay.ConclusionWith a simplified workflow, N1-STOP-LAMP is a powerful, scalable option for specific and rapid detection of SARS-CoV-2 and an additional resource in the diagnostic armamentarium against COVID-19.3.Data summaryThe authors confirm all supporting data, code and protocols have been provided within the article or through supplementary data files.


2019 ◽  
Vol 551 ◽  
pp. 101-110 ◽  
Author(s):  
Bei-Bei Wang ◽  
Jian-Cheng Jin ◽  
Zi-Qiang Xu ◽  
Zi-Wen Jiang ◽  
Xun Li ◽  
...  

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