RNA Cancer Nanomedicine: Nanotechnology-mediated RNA Therapy

Nanoscale ◽

10.1039/d1nr06991h ◽

2022 ◽

Author(s):

Bijan Emiliano Ferdows ◽

Dylan Neal Patel ◽

Wei Chen ◽

Xiangang Huang ◽

Na Kong ◽

...

Keyword(s):

Tumor Progression ◽

Rna Molecules ◽

Cancer Nanomedicine

It has been demonstrated that RNA molecules—mRNA, siRNA, microRNA, and sgRNA—regulate cancer-specific genes, and therefore, RNA-based therapeutics can suppress tumor progression and metastasis by selectively upregulating and silencing these genes....

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Exosome-Mediated Signaling in Epithelial to Mesenchymal Transition and Tumor Progression

Journal of Clinical Medicine ◽

10.3390/jcm8010026 ◽

2018 ◽

Vol 8 (1) ◽

pp. 26 ◽

Cited By ~ 23

Author(s):

Alice Conigliaro ◽

Carla Cicchini

Keyword(s):

Tumor Progression ◽

Intercellular Communication ◽

Epithelial To Mesenchymal Transition ◽

Current Knowledge ◽

Cell Communication ◽

Therapeutic Approaches ◽

Mesenchymal Transition ◽

Rna Molecules ◽

Dna And Rna

Growing evidence points to exosomes as key mediators of cell–cell communication, by transferring their specific cargo (e.g., proteins, lipids, DNA and RNA molecules) from producing to receiving cells. In cancer, the regulation of the exosome-mediated intercellular communication may be reshaped, inducing relevant changes in gene expression of recipient cells in addition to microenvironment alterations. Notably, exosomes may deliver signals able to induce the transdifferentiation process known as Epithelial-to-Mesenchymal Transition (EMT). In this review, we summarize recent findings on the role of exosomes in tumor progression and EMT, highlighting current knowledge on exosome-mediated intercellular communication in tumor-niche establishment, migration, invasion, and metastasis processes. This body of evidence suggests the relevance of taking into account exosome-mediated signaling and its multifaceted aspects to develop innovative anti-tumoral therapeutic approaches.

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Electron Microscopy of the Nucleic Acid of Mouse Mammary Tumor Virus

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100099854 ◽

1968 ◽

Vol 26 ◽

pp. 22-23

Author(s):

N. H. Sarkar ◽

Dan H. Moore

Keyword(s):

Mammary Tumor ◽

Mouse Mammary Tumor Virus ◽

Ammonium Acetate ◽

Dry Weight ◽

Mouse Tumor ◽

Mammary Tumor Virus ◽

Rna Molecules ◽

Mouse Mammary Tumor ◽

Tumor Virus ◽

The Subject

Mouse mammary tumor virus (MTV) is believed to contain about 0.8% single stranded ribonucleic acid (RNA). This value of RNA content was estimated on a dry weight basis. The subject of this report is an attempt to visualize the RNA molecules of MTV particles.MTV particles were isolated from RIII mouse (tumor incidence approximately 80%) milk according to the method described by Lyons and Moore. Purified virions from 5 ml of milk were finally suspended in 0.2 ml of PBS, pH 7.4 and was mixed with an equal volume of pronase (5 mg/ml). This mixture was incubated at 37°C for an hour. RNA was extracted three times using freshly prepared cold phenol. It was then treated three times with cold ethyl ether to remove any trace of phenol. The RNA thus extracted was divided into two parts. One part was diluted four fold with 8M urea to avoid aggregation of the molecules. The other part was left untreated. Both samples were then mixed with an equal volume of 1M ammonium acetate, adjusted to pH 8.0 with NH3 containing chymotrypsin at a concentration of 0.01%.

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Electron Microscope Study of RNA's of Paramyxoviruses

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100094231 ◽

1972 ◽

Vol 30 ◽

pp. 196-197

Author(s):

Ruchama Baum ◽

J.T. Seto

Keyword(s):

Electron Microscopy ◽

Electron Microscope ◽

Electron Microscope Study ◽

Sendai Virus ◽

Sodium Dodecyl ◽

Rna Molecules ◽

Virus Particles ◽

Molecular Weights ◽

Length Measurements

The ribonucleic acid (RNA) of paramyxoviruses has been characterized by biochemical and physiochemical methods. However, paramyxovirus RNA molecules have not been studied by electron microscopy. The molecular weights of these single-stranded viral RNA molecules are not known as yet. Since electron microscopy has been found to be useful for the characterization of single-stranded RNA, this investigation was initiated to examine the morphology and length measurements of paramyxovirus RNA's.Sendai virus Z strain and Newcastle disease virus (NDV), Milano strain, were used. For these studies it was necessary to develop a method of extracting RNA molecules from purified virus particles. Highly purified Sendai virus was treated with pronase (300 μg/ml) at 37°C for 30 minutes and the RNA extracted by the sodium dodecyl sulfate (SDS)-phenol procedure.

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