scholarly journals Label-free quantitative proteomics analysis of jujube (Ziziphus jujuba Mill.) during different growth stages

RSC Advances ◽  
2021 ◽  
Vol 11 (36) ◽  
pp. 22106-22119
Author(s):  
Xiaoli Huang ◽  
Zhaohua Hou
Keyword(s):  
Quantitative Proteomics ◽  
Growth Stage ◽  
Growth Stages ◽  
Label Free ◽  
Flavor Quality ◽  
Chinese Jujube ◽  
Proteomics Analysis ◽  
Zizyphus Jujuba ◽  
Ziziphus Jujuba ◽  
Different Growth Stages

Chinese jujube (Zizyphus jujuba Mill.), a member of the Rhamnaceae family with favorable nutritional and flavor quality, exhibited characteristic climacteric changes during its fruit growth stage.

scholarly journals Comparative Proteomics Analysis of Mouse Habu Nephritis Models with and without Unilateral Nephrectomy

2016 ◽  
Vol 39 (5) ◽  
pp. 1761-1776 ◽  
Author(s):  
Lei Chen ◽  
Yang Lu ◽  
Jun Wen ◽  
Xu Wang ◽  
Lingling Wu ◽  
...  
Keyword(s):  
Renal Injury ◽  
Quantitative Proteomics ◽  
Mesangial Cells ◽  
Unilateral Nephrectomy ◽  
Label Free ◽  
Proteomics Analysis ◽  
Single Kidney ◽  
Regulation Of Actin Cytoskeleton ◽  
Insight Into

Background/Aims: Individuals possessing a single kidney are at greater risk of renal injury upon exposure to harmful stimuli. This study aimed to explore the pathogenesis of renal injury in glomerulonephritis with versus without unilateral nephrectomy (UNX). Methods: Histological analysis and label-free quantitative proteomics were performed on two models—the Habu snake venom-induced glomerulonephritis model with versus without UNX (HabuU and Habu models, respectively). The role of villin 1, a differentially expressed protein (DEP) in mouse mesangial cells, was investigated. Results: Persistent mesangiolysis and focal hypercellularity together with reduced activation of cell proliferation in the HabuU model induced more serious renal injury compared with that in the Habu model. The DEPs between the two models were identified by label-free liquid chromatography-mass spectrometry. The KEGG pathway results indicated that regulation of actin cytoskeleton and focal adhesion were specifically enriched in the HabuU model. The cytoskeleton regulation protein villin 1 was downregulated in the HabuU model, but unchanged in the Habu model. Knockdown of villin 1 promoted apoptosis and inhibited the proliferation of mouse mesangial cells, suggesting villin 1 to be involved in qlomerular lesion self-repair insufficiency. Conclusion: By assessing the proteomic profiles of the two models, this study identified several important differences, particularly villin 1 expression, in regulatory mechanisms between the two models. Our findings provide novel insight into the mechanism of serious renal injury in glomerulonephritis with UNX.

scholarly journals Evaluation of Rice Responses to the Blast Fungus Magnaporthe oryzae at Different Growth Stages

Plant Disease ◽  
2019 ◽  
Vol 103 (1) ◽  
pp. 132-136 ◽  
Author(s):  
Xinglong Chen ◽  
Yulin Jia ◽  
Bo Ming Wu
Keyword(s):  
Disease Severity ◽  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Growth Stage ◽  
Disease Index ◽  
Blast Disease ◽  
Growth Stages ◽  
Japonica Rice ◽  
Rice Cultivars ◽  
Different Growth Stages

Rice blast, caused by the fungus Magnaporthe oryzae, is the most damaging disease for rice worldwide. However, the reactions of rice to M. oryzae at different growth stages are largely unknown. In the present study, two temperate japonica rice cultivars, M-202 and Nipponbare, were inoculated synchronously at different vegetative growth stages, V1 to V10. Plants of M-202 at each stage from V1 to reproductive stage R8 were inoculated with M. oryzae race (isolate) IB-49 (ZN61) under controlled conditions. Disease reactions were recorded 7 days postinoculation by measuring the percentage of diseased area of all leaves, excluding the youngest leaf. The results showed that the plants were significantly susceptible at the V1 to V4 stages with a disease severity of 26.7 to 46.8% and disease index of 18.62 to 37.76 for M-202. At the V1 to V2 stages, the plants were significantly susceptible with a disease a severity of 28.6 to 39.3% and disease index of 23.65 to 29.82 for Nipponbare. Similar results were observed when plants of M-202 were inoculated at each growth stage with a disease severity of 29.7 to 60.6% and disease index of 21.93 to 59.25 from V1 to V4. Susceptibility decreased after the V5 stage (severity 4.6% and index 2.17) and became completely resistant at the V9 to V10 stages and after the reproductive stages, suggesting that plants have enhanced disease resistance at later growth stages. These findings are useful for managing rice blast disease in commercial rice production worldwide.

Irrigation of field peas (Pisum sativum): response to timing at different crop growth stages

1999 ◽  
Vol 132 (4) ◽  
pp. 417-424 ◽  
Author(s):  
C. M. KNOTT
Keyword(s):  
Pisum Sativum ◽  
Vegetative Growth ◽  
Growth Stage ◽  
Crop Growth ◽  
Yield Response ◽  
Growth Stages ◽  
Field Peas ◽  
Crop Growth Stages ◽  
Different Growth Stages

The response of two cultivars of dry harvest field peas (Pisum sativum), Solara and Bohatyr, to irrigation at different growth stages was studied on light soils overlying sand in Nottinghamshire, England in 1990, when the spring was particularly dry, in 1991 which had a dry spring and summer and in contrast, 1992, when rainfall was greater compared with the long-term (40 year) mean.Solara, short haulmed and semi-leafless was more sensitive to drought than the tall conventional-leaved cultivar Bohatyr and gave a greater yield response to irrigation, particularly at the vegetative growth stage in the first two dry years 1990 and 1991, of 108% and 55% respectively, compared with unirrigated plots. Bohatyr was less sensitive to the timing of single applications.In all years, peas irrigated throughout on several occasions produced the highest yields, but this was the least efficient use of water.

Nutrient digestibility of alfalfa at different growth stages on sheep and goat

2003 ◽  
Vol 2003 ◽  
pp. 193-193
Author(s):  
M. M. Moeini ◽  
M. Souri ◽  
F. Hozabri ◽  
M. R. Sanjabi
Keyword(s):  
Dry Matter ◽  
Growth Stage ◽  
Animal Feed ◽  
Maximum Yield ◽  
Nutrient Digestibility ◽  
Growth Stages ◽  
Preparation Methods ◽  
Nutritive Values ◽  
High Forage ◽  
Different Growth Stages

The nutritive values of animal feed are dependents on plant species, stages of maturity, harvesting and preparation methods. Legumes provide maximum yield, high forage quality (protein, mineral and digestible energy). Legumes decrease in protein and digestible dry matter and increase in fibre as they increase in growth or in maturity (Hochensmith et al., 1997). Alfalfa (medica sativa) is world unique forage in livestock food. This study was conducted to examine the chemical composition and nutrient digestibility of Hamadanian alfalfa forage at different growth stage on two local Iranian sheep and goat breeds.

scholarly journals Salmonella in healthy pigs: prevalence, serotype diversity and antimicrobial resistance observed during 1998–1999 and 2004–2005 in Japan

2007 ◽  
Vol 136 (8) ◽  
pp. 1118-1123 ◽  
Author(s):  
K. FUTAGAWA-SAITO ◽  
S. HIRATSUKA ◽  
M. KAMIBEPPU ◽  
T. HIROSAWA ◽  
K. OYABU ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Growth Stage ◽  
Growth Stages ◽  
Finishing Pigs ◽  
Weaned Pigs ◽  
Farm Level ◽  
Faecal Samples ◽  
National Estimates ◽  
Fattening Pigs ◽  
Different Growth Stages

SUMMARYTo determine prevalence, serotype diversity and antimicrobial resistance of Salmonella in healthy pigs, faecal samples from 6771 pigs on 73 farms collected during 1998–1999 and 2004–2005 were examined. Salmonella isolates were serotyped and tested for susceptibility to 22 antimicrobials: benzylpenicillin, ampicillin, amoxicillin, cefazolin, cephaloridine, gentamicin, kanamycin, streptomycin, fradiomycin, colistin, tetracycline, chlortetracycline, oxytetracycline, chloramphenicol, thiamphenicol, sulfadimethoxine, sulfamethoxazole, sulfamethoxypyridazine, trimethoprim, sulfamethoxazole/trimethoprim, norfloxacin and ofloxacin. Farm-level and pig-level Salmonella prevalences were 35·5% and 2·2% in 1998–1999, and 35·7% and 3·3% in 2004–2005. Prevalence by growth stage was 2·4% for sows, 3·3% for weaned pigs, 2·7% for fattening pigs and 3·8% for finishing pigs. The predominant serotypes identified were Agona (28·4%), Typhimurium (17·9%) and Infantis (16·4%) in 1998–1999, and Typhimurium (32·5%), Anatum (24·6%) and Infantis (13·5%) in 2004–2005. Compared with the 1998–1999 isolates, the 2004–2005 isolates showed significantly higher rates of resistance to all the antimicrobials except tetracyclines (P<0·01 to P<0·05) and resistance to ⩾2 antimicrobials [19·4% (13/67) vs. 39·7% (50/126), P<0·01]. This study provides national estimates of Salmonella prevalence in healthy pigs of different growth stages in Japan.

scholarly journals The effect of lipocalin-2 (LCN2) on apoptosis: a proteomics analysis study in an LCN2 deficient mouse model

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Dongming Wu ◽  
Xiaopeng Wang ◽  
Ye Han ◽  
Yayun Wang
Keyword(s):  
Knockout Mice ◽  
Quantitative Proteomics ◽  
Energy Homeostasis ◽  
Metabolic Diseases ◽  
Differentially Expressed Proteins ◽  
Label Free ◽  
Lipocalin 2 ◽  
Wild Type ◽  
Proteomics Analysis ◽  
Associated Proteins

Abstract Background Recent studies have shown that lipocalin-2 (LCN2) has multiple functions involved in various biological and pathological processes including energy homeostasis, cancer, inflammation, and apoptosis. We aimed to investigate the effect of LCN2 on apoptosis that influences the pathogenetic process of metabolic diseases and cancer. Methods We performed a proteomics analysis of livers taken from LCN2-knockout mice and wild type mice by using label-free LC-MS/MS quantitative proteomics. Results Proteomic analysis revealed that there were 132 significantly differentially expressed proteins (49 upregulated and 83 downregulated) among 2140 proteins in the liver of LCN2-knockout mice compared with wild type mice. Of these, seven apoptosis-associated proteins were significantly upregulated and seven apoptosis-associated proteins downregulated. Conclusion Proteomics demonstrated that there were seven upregulated and seven downregulated apoptosis-associated proteins in liver of LCN2-knockout mice. It is important to clarify the effect of LCN2 on apoptosis that might contribute to the pathogenesis of insulin resistance, cancer, and various nervous system diseases.

scholarly journals Effect of Different Durations of Water-Logging at Different Growth Stages on Seed Yield of Sesame

2018 ◽  
Vol 1 (2) ◽  
pp. p68
Author(s):  
M. H. Ali
Keyword(s):  
Seed Yield ◽  
Growth Stage ◽  
Field Trials ◽  
Growth Stages ◽  
Differential Effects ◽  
Growing Period ◽  
Water Logging ◽  
Different Growth Stages

In Bangladesh, sesame suffers from water-logging during its growing period. Multi-year and multi-location field trials were carried out to study the effect of different durations of water-logging at different growth stages on seed yield of sesame. From two years results, it is revealed that the effects of water-logging during a particular growth stage or particular duration of water-logging on seed yield depends on pre- and/or post water-logging from the rainfall. Differential effects of the cultivars were also observed. The cultivar Binatil-2 and Binatil-3 showed reasonable seed yield under water-logging at flowering and mid pod-formation stages for 24 to36 hours.

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