scholarly journals Abscisic acid-determined seed vigour differences do not influence redox regulation during ageing

2019 ◽  
Vol 476 (6) ◽  
pp. 965-974 ◽  
Author(s):  
Christina Schausberger ◽  
Thomas Roach ◽  
Wolfgang Stöggl ◽  
Erwann Arc ◽  
William E. Finch-Savage ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Redox Regulation ◽  
Seed Quality ◽  
Seed Viability ◽  
Seed Storage ◽  
Storage Conditions ◽  
Seed Vigour ◽  
Allelic Differences ◽  
Seed Ageing ◽  
A Minor

Abstract High seed quality is a key trait to achieve successful crop establishment required for optimum yield and sustainable production. Seed storage conditions greatly impact two key seed quality traits; seed viability (ability to germinate and produce normal seedlings) and vigour (germination performance). Accumulated oxidative damage accompanies the loss of seed vigour and viability during ageing, indicating that redox control is key to longevity. Here, we studied the effects of controlled deterioration at 40°C and 75% relative humidity (RH) (‘ageing’) under two different O2 concentrations (21 and 78% O2) in Brassica oleracea. Two B. oleracea genotypes with allelic differences at two QTLs that result in differences in abscisic acid (ABA) signalling and seed vigour were compared. Ageing led to a similar loss in germination speed in both genotypes that was lost faster under elevated O2. In both genotypes, an equal oxidative shift in the glutathione redox state and a minor loss of α-tocopherol progressively occurred before seed viability was lost. In contrast, ABA levels were not affected by ageing. In conclusion, both ABA signalling and seed ageing impact seed vigour but not necessarily through the same biochemical mechanisms.

Evaluation of Physiological Quality of Seeds of Improved Snap Bean Lines under Different Storage

2019 ◽  
pp. 1-10
Author(s):  
Camila Queiroz da Silva Sanfim de Santan ◽  
Luana Barros Freitas ◽  
Tâmara Rebecca Albuquerque de Oliveira ◽  
Jocarla Ambrosim Crevelari ◽  
Derivaldo Pureza da Cruz ◽  
...  
Keyword(s):  
Seed Quality ◽  
Block Design ◽  
Seed Viability ◽  
Seed Storage ◽  
Storage Conditions ◽  
Snap Bean ◽  
Different Types ◽  
Physiological Quality ◽  
And Storage

Seed quality is a key factor to succeed in a yield generation and storage is an important activity to control the physiological seed quality, as it preserves seed viability while maintaining its robustness at a reasonable level between planting and harvest. On this basis, the goal of this study was to evaluate the physiological quality of snap bean seeds stored in different types of packages and storage conditions. Eight genotypes comprising six lines and two controls were analyzed. It was applied a completely randomized block design with four replicates in a factorial scheme (8×2×2×7).Seed storage was performed in permeable and impermeable packages under controlled and uncontrolled conditions. The physiological seed quality was evaluated by means of germination and vigor tests. The different types of package and storage conditions influenced the physiological seed quality. Under controlled storage conditions, the permeable and impermeable packages were more efficient in controlling the physiological quality of snap bean seeds.

High seed viability recorded in an endangered endemic species, 'Isoplexis isabelliana' (Scrophulariaceae), after more than 30 years of storage in a conservation seed bank

2021 ◽  
Vol 42 ◽  
pp. e69341
Author(s):  
Miguel Ángel González Pérez ◽  
Nereida Cabrera-García ◽  
Isabel Cayon-Fernández
Keyword(s):  
Seed Bank ◽  
Germination Rate ◽  
Genetic Material ◽  
Natural Populations ◽  
Seed Viability ◽  
Seed Storage ◽  
Botanical Garden ◽  
Storage Conditions ◽  
Seed Banks ◽  
Ex Situ

Conservation seed banks are essential for ex-situ conservation of genetic biodiversity. These institutions are especially relevant for threatened species and play a vital role in their conservation by preserving genetic material. However, samples deposited in the seed banks must germinate when necessary to use them (i.e., recovery plans, etc.). This study uses four accessions of the endemic endangered species from Gran Canaria Island (Canary Islands), Isoplexis isabelliana (Webb & Berthel.) Masf. (Scrophulariaceae). Germination tests were carried out to measure seed viability through time and the possible impact of seed storage on their viability. These accessions have been kept in the seed bank for four months to thirty years under different storage conditions. Germination results differed for seeds after 45 days of exposition using 16 hours light and 8 hours darkness at 17 °C. Accessions kept in the seed bank, independently of storage, showed a high germination percentage (89%). Whereas the accessions with rough storage conditions showed a 0% germination rate. The results highlighted the good state of conservation of the material deposited in the Seed Bank of the Botanical Garden "Viera y Clavijo" and the reliability of the temperature and humidity conditions in which the seeds of I. isabelliana have been stored. We consider these results as momentous since several natural populations of I. isabelliana has been affected by the last forest fire on the island.

Effect of NPK on growth, yield and seed quality of hybrid Chilli

2019 ◽  
Vol 9 (1) ◽  
pp. 35-41
Author(s):  
M Hasan ◽  
M Robbani ◽  
R Parvin ◽  
MNH Mehedi ◽  
I Hossain
Keyword(s):  
Seed Yield ◽  
Seed Quality ◽  
Seed Viability ◽  
Growth Yield ◽  
Control Treatment ◽  
Npk Fertilizers ◽  
Seed Vigour ◽  
Female Line ◽  
Long Time

An experiment was carried out at Germplasm Centre and Plant Biotechnology Lab, Department of Horticulture, PSTU to evaluate the growth, seed yield and quality of hybrid F1 chilli (cv. Sonic) crossing male line (LTSL-004-M) and female line (LTSL-004-F) along the viability of productive seeds influenced by NPK fertilizers during the period from November 2015 to October 2016. Ten different treatments of NPK fertilizers were used. Result showed that all the characteristics except primary branches and1000 seed weight were influenced significantly due to the application of NPK fertilizers. The tallest plant (69.88 cm), more leaves plant–1 (2491.33), highest number of secondary branches plant-1, tertiary branches plant-1 (5.00 and 88.00, respectively), number of flowers and hybrid fruits plant–1 (1533.00 and 12.33, respectively) and weight of seeds (4.03 g plant–1) along with required more time (54.67 days) for flowering were obtained from the application of 145 kg N ha–1, 175 kg P ha–1 and 96 kg K ha–1 (T4) which produced the highest final seed yield of chilli (4.03 kg ha–1). Application of T5 and T9 showed the highest number of primary branches (2.33). In seed viability characteristics T9 treated seeds showed the highest germination (100, 97.33 and 94.97%) at 1st, (1 MAH - Month After Harvest), 2nd (2 MAH) and 3rd (4 MAH) observations, respectively. The T3 treated seeds performed well in respect of Seed Vigour Index (SVI) at 1st and 2nd observations (8.00 and 7.48, respectively) but T9 treated seeds showed the highest SVI (6.32) at 3rd observation. Above indicating all characteristics were lowest under only recommended doses of NPK T1 as control treatment except days to first flowering. The observations suggested that production of hybrid chilli seeds could be enhanced by applying T4. While seeds of chilli treated by T9 in field level showed long time viable. Application of 145 kg N ha–1, 175 kg P ha–1 and 96 kg K ha–1 may be suggested for seed production of chilli and 110 kg N ha–1, + 175 kg P ha–1 + 115 kg K ha–1 for keeping the long time seed viability. Int. J. Agril. Res. Innov. & Tech. 9 (1): 35-41, June, 2019

scholarly journals Primed Lettuce Seeds Exhibit Increased Sensitivity to Moisture Content During Controlled Deterioration

HortScience ◽  
2007 ◽  
Vol 42 (6) ◽  
pp. 1436-1439 ◽  
Author(s):  
H.J. Hill ◽  
Jesse D. Cunningham ◽  
Kent J. Bradford ◽  
A.G. Taylor
Keyword(s):  
Moisture Content ◽  
Elevated Temperatures ◽  
Seed Viability ◽  
Seed Storage ◽  
Seed Priming ◽  
Storage Conditions ◽  
Seed Moisture Content ◽  
Moisture Contents ◽  
Lettuce Seeds ◽  
Rate Of Decline

The Ellis-Roberts seed viability equation is used to predict seed survival after storage at specified temperatures and moisture contents. Seed priming, which can break dormancy and accelerate germination, can also reduce seed storage life. Because primed seeds were not used in developing the Ellis-Roberts equation, the reciprocal nature of specific seed moisture content (MC, fresh weight basis) and temperatures that applies to nonprimed lettuce (Lactuca sativa L.) seeds may not apply to primed seeds. To determine how priming affects lettuce seeds in relation to the viability equation, an experiment was conducted using two cultivars, ‘Big Ben’ and ‘Parris Island Cos’. Seeds primed in polyethylene glycol 8000 (–1.45 MPa, 24 h at 15 °C) and nonprimed seeds were first adjusted to 6% and 9% moisture contents and then stored at 48 and 38 °C for up to 30 days, respectively. These storage conditions (6% MC and 48 °C; 9% MC and 38 °C) were predicted by the viability equation to result in equal longevities. Subsequent viability assays at 20 °C revealed that nonprimed seeds in both storage environments exhibited similar losses in viability over time, thus validating the Ellis-Roberts equation and the use of these conditions to apply different but equal aging stress. Primed seeds of both cultivars deteriorated faster than nonprimed seeds as expected. However, primed seeds did exhibit different rates of deterioration between the storage environments. Primed seeds stored at 9% MC and 38 °C deteriorated faster than primed seeds stored at 6% MC and 48 °C. The rate of decline in probit viability percentage was three times greater in primed ‘Big Ben’ seeds stored at 9% MC and 38 °C than for those stored at 6% MC and 48 °C (–1.34 versus –0.26 probits per day, respectively). ‘Parris Island Cos’ seeds stored at 9% MC and 38 °C had twice the rate of deterioration that those stored at 6% MC and 48 °C (–1.19 and –0.49 probits per day, respectively). The results indicate that primed lettuce seeds were more sensitive to the adverse effects of higher seed MC than were nonprimed seeds during storage at elevated temperatures.

scholarly journals Reserve mobilization during imbibition of stored Gliricidia sepium (Jacq.) Steud. (Leguminosae - Papilionoideae) seeds

2011 ◽  
Vol 33 (3) ◽  
pp. 549-560
Author(s):  
Renata Conduru Ribeiro Reis ◽  
Bárbara França Dantas ◽  
Renato Delmondez de Castro ◽  
Cimille Gabrielle Cardoso Antunes ◽  
Fabrício Francisco Santos da Silva ◽  
...  
Keyword(s):  
Soluble Sugars ◽  
Seed Viability ◽  
Storage Period ◽  
Seed Storage ◽  
Gliricidia Sepium ◽  
Storage Conditions ◽  
Ambient Conditions ◽  
Total Soluble Sugars ◽  
Drought Tolerant ◽  
Semi Arid Region

Gliricidia sepium is a drought-tolerant species, easily multiplied by seeds, and has been exploited by farmers as a source of forage in the semi-arid region of northeast Brazil. The objective of the present study was to evaluate the effect of seed storage on the mobilization of reserves during imbibition of "Gliricidia" seeds. Freshly-harvested seeds were packed in kraft paper bags and stored for three and six months in the laboratory under ambient conditions (25 º C ± 3 T and 75% ± 3 RH). Cotyledons were isolated from imbibed seeds and macerated for the extraction and quantification of total soluble sugars, reducing sugars, sucrose and starch, as well as of proteins, amino acids and for amylase activity. Storage under these conditions resulted in an increase in seed water content although germination remained at relatively high levels (86%). Seed macromolecule levels showed significant variation with the storage period and imbibition and these variations were associated with a loss in seed viability due to inadequate storage conditions.

scholarly journals EFFECT OF STORAGE CONTAINERS AND SEED TREATMENTS ON SEED GERMINATION AND VIGOUR OF BLACK GRAM (VIGNA MUNGO (L.) HEPPER)

2020 ◽  
Vol 21 (supplement 1) ◽  
Author(s):  
J.Johnny subakar ivin ◽  
Y.Anbuselvam subakar ivin ◽  
M.Surendhar J.R. Jerish ◽  
M.Surendhar J.R. Jerish
Keyword(s):  
Shelf Life ◽  
Seed Quality ◽  
Seed Viability ◽  
Seed Storage ◽  
Wire Mesh ◽  
Monthly Interval ◽  
Black Gram ◽  
Neem Oil ◽  
The Poor ◽  
Vigour Index

One of the most important basic needs for higher agricultural production is quality seed, characterized by high viability and vigour. Maintenance of seed viability and vigour from Harvest till the next growing season is of the utmost importance in a seed production programme. During seed storage, qualitative and quantitative losses of up to 20% have been reported in India. The poor seed quality may also be due to the poor storability which is very often being decided by the internal and external factors. In pulses, the major cause for seed deterioration during storage is bruchid damage. In this context, evolving an improved storage strategy to prolong the shelf life of seeds under ambient storage conditions with easily available cost-effective resources was carried out. The experiment was conducted at the Department of Genetics and Plant Breeding, Faculty of Agriculture, Annamalai University, Annamalai nagar. Freshly harvested seeds of black gram cv. ADT 3 were cleaned and graded using BSS, 8x8 wire mesh sieve. Then seeds were dried under sunlight to bring the moisture content to required level (8.5%). The seeds were treated with Thiram @ 2g kg-1, Neem oil @ 10ml kg-1 and Malathion dust @ 200 mg kg-1. Two hundred and fifty grams of seeds from all treatments along with control were packed in two containers viz. cloth bag and polythene bag (700 gauges) with three replication and stored in at ambient temperature (30o c – 35o c) and relative humidity of 52-80%. Seed samples were drawn from each replication at monthly interval for assessing the viability and vigour. Germination test was conducted in between paper method and seedlings were evaluated on seventh day for vigour index bruchid infestation. The untreated seeds recorded lower germination per cent and vigour index after seven months of storage. There was no bruchid damage on seeds treated with neem oil @ 10ml kg-1 and thiram @ 2g kg-1. It was concluded that the shelf life of black gram seeds could be increased by

Seed survival in Chilean Nothofagus in response to desiccation and storage

2005 ◽  
Vol 15 (2) ◽  
pp. 113-123 ◽  
Author(s):  
Pedro León-Lobos ◽  
Richard H. Ellis
Keyword(s):  
Moisture Content ◽  
Seed Quality ◽  
Seed Viability ◽  
Seed Storage ◽  
Forest Tree ◽  
Ex Situ ◽  
Hermetic Storage ◽  
Moisture Contents ◽  
Tree Seeds ◽  
Initial Seed

Nothofagus alpina,N. obliqua,N. glauca,N. leonii,N. dombeyiandN. pumilioseeds exhibited consistent, albeit slight, sensitivity to extreme desiccation, but nevertheless maintained viability at low moisture contents and cool temperatures (–10° to –20°C) over 2 years.Nothofagus alpina,N. obliqua,N. glauca,N. leoniiandN. dombeyiconformed to the seed viability equation of Ellis and Roberts; sensitivity of longevity to temperature was quantitatively similar to that of crop seeds, sensitivity to moisture was somewhat less, and a low-moisture-content limit to the equation was detected at 4.8% moisture content in hermetic storage at 65 °C, and possibly similar moisture contents at 30–40°C. These five species show orthodox seed storage behaviour. Therefore,ex-situconservation of theseNothofagusspecies in seed banks is possible, but the quality of seed lots collected requires attention. Seed storage behaviour was not defined inN. pumilio: initial seed quality was poor and loss of viability was detected over 2 years at 0°, –10° and –20°C at 2.7% moisture content, but not at 5.2%. The results confirm that the economy of nature in seed storage physiology extends to forest tree seeds, but the repeated observation of reduced sensitivity of longevity to moisture in forest tree seeds requires further investigation.

scholarly journals Alleviate Seed Ageing Effects in Silybum marianum by Application of Hormone Seed Priming

2015 ◽  
Vol 7 (3) ◽  
pp. 316-321
Author(s):  
Seyed Ata SIADAT ◽  
Seyed Amir MOOSAVI ◽  
Mehran SHARAFIZADEH
Keyword(s):  
Seed Germination ◽  
Seed Quality ◽  
Morphological Structure ◽  
Seed Priming ◽  
Silybum Marianum ◽  
Duration Of Treatment ◽  
Seed Vigour ◽  
Germination Characteristics ◽  
Poor Seed ◽  
Seed Ageing

Most of the medicinal plants have seed dormancy or poor seed germination. This is due to their natural compounds or their morphological structure. Existence of such mechanisms makes the seeds able to endure harsh environments, stressful conditions or natural ageing. Different seed enhancement treatments were proposed in order to improve seed germination. In this study, it was examined Silybum marianum seed longevity (0, 48 and 72 hour of ageing) and the response to seed priming when using different concentrations of cytokinin (50, 200, 350 and 500 ppm) and different duration of treatment (8, 12 and 24 hour). Results revealed that ageing could be successfully alleviated using seed priming. Seed ageing significantly affected seed germination and vigour. Application of cytokinin considerably improved seed vigour in aged and non aged seeds. The most effective seed priming treatment was with 500 ppm cytokine, with the duration of 24 hours. In conclusion, it can be recommend the used of plant growth regulators like cytokine, as a good priming agent, to recover losses of seed quality and improve germination characteristics.

Effect of Cone Storage on Pine Seed Storage Potential

1991 ◽  
Vol 15 (4) ◽  
pp. 216-221 ◽  
Author(s):  
F. T. Bonner
Keyword(s):  
Seed Quality ◽  
Germination Rate ◽  
Seed Viability ◽  
Seed Storage ◽  
Slash Pine ◽  
White Pine ◽  
Storage Container ◽  
Percent Germination ◽  
Pinus Taeda L ◽  
One Year

Abstract The effect of cone storage on seed viability during 5 years of storage at 35°F was tested with seeds from studies with loblolly (Pinus taeda L.), slash (P. elliottii Engelm.), longleaf (P. palustris Mill.), and eastern white (P. strobus L.) pines. Results showed that: (1) with the exception of slash pine, seeds from cones stored for the longer periods retained quality during storage better than seeds from cones that were stored only short periods; (2) all species exhibited slight, but statistically significant, decreases in seed quality after 3 years of storage, and with the exception of loblolly, more substantial decreases in germination rate at 5 years; (3) with the exception of eastern white pine, declining rates of germination indicated losses of seed quality at least one year before total percent germination decreased, and (4) slash pine seeds from cones in the center of 20-bushel crates performed better in storage than those from cones at the top of the crates or from cones stored in bags, but storage container made no difference for loblolly. South. J. Appl. For. 15(4):216-221

Watching the days go by: Aging during sunflower seed storage under distinct oxygen availability

2021 ◽  
Vol 45 ◽  
Author(s):  
Rafael Agostinho Ferreira ◽  
Alisson Alves de Oliveira ◽  
Victor Navarro da Silva ◽  
Aline Aparecida Silva Pereira ◽  
Mateus Moreira Bernardes ◽  
...  
Keyword(s):  
Aging Process ◽  
Seed Viability ◽  
Seed Storage ◽  
Storage Conditions ◽  
Oxygen Availability ◽  
Low Oxygen ◽  
Experimental Conditions ◽  
Oxygen Conditions ◽  
Mda Content ◽  
Over Time

ABSTRACT The maintenance of seed viability is widely studied since preserving the physiological characteristics that will allow efficient germination and adequate field occupation is broadly pursued. However, even under optimal storage conditions, the aging process is inherent to the seed’s life. In order to understand the effects of storage under low and normal oxygen conditions, this work sought to evaluate the physiological responses of two seed lots of two sunflower hybrids stored under different oxygen availability (normoxia and hypoxia) over a 360-day period. Aiming to investigate the effects of storage, the activities of the enzymatic antioxidant metabolism, hydrogen peroxide and MDA content, and the performance of viability, and vigor tests (tetrazolium test and electrolyte leakage) were performed with the stored seeds every 60 days. The hypoxia conditions were not able to keep seed viability over time, probably affecting negatively the embryonic axis. Throughout the evaluations, the viability tests demonstrated that the storage in the two experimental conditions was not able to contain the aging of the seeds. The increased content of H2O2 and MDA, associated with the enhanced electrical conductivity over time, indicate that there were losses by lipid peroxidation and that the aging process was not contained by storage under low oxygen availability.

Sign in / Sign up

Export Citation Format

Share Document