Seed survival in Chilean Nothofagus in response to desiccation and storage

2005 ◽  
Vol 15 (2) ◽  
pp. 113-123 ◽  
Author(s):  
Pedro León-Lobos ◽  
Richard H. Ellis
Keyword(s):  
Moisture Content ◽  
Seed Quality ◽  
Seed Viability ◽  
Seed Storage ◽  
Forest Tree ◽  
Ex Situ ◽  
Hermetic Storage ◽  
Moisture Contents ◽  
Tree Seeds ◽  
Initial Seed

Nothofagus alpina,N. obliqua,N. glauca,N. leonii,N. dombeyiandN. pumilioseeds exhibited consistent, albeit slight, sensitivity to extreme desiccation, but nevertheless maintained viability at low moisture contents and cool temperatures (–10° to –20°C) over 2 years.Nothofagus alpina,N. obliqua,N. glauca,N. leoniiandN. dombeyiconformed to the seed viability equation of Ellis and Roberts; sensitivity of longevity to temperature was quantitatively similar to that of crop seeds, sensitivity to moisture was somewhat less, and a low-moisture-content limit to the equation was detected at 4.8% moisture content in hermetic storage at 65 °C, and possibly similar moisture contents at 30–40°C. These five species show orthodox seed storage behaviour. Therefore,ex-situconservation of theseNothofagusspecies in seed banks is possible, but the quality of seed lots collected requires attention. Seed storage behaviour was not defined inN. pumilio: initial seed quality was poor and loss of viability was detected over 2 years at 0°, –10° and –20°C at 2.7% moisture content, but not at 5.2%. The results confirm that the economy of nature in seed storage physiology extends to forest tree seeds, but the repeated observation of reduced sensitivity of longevity to moisture in forest tree seeds requires further investigation.

Seed storage behaviour in Elaeis guineensis

1991 ◽  
Vol 1 (2) ◽  
pp. 99-104 ◽  
Author(s):  
R. H. Ellis ◽  
T. D. Hong ◽  
E. H. Roberts ◽  
U. Soetisna
Keyword(s):  
Moisture Content ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Seed Viability ◽  
Seed Storage ◽  
Freezing Damage ◽  
Hermetic Storage ◽  
Moisture Contents ◽  
Storage Temperatures

AbstractSeed viability was maintained in four cultivars of oil palm (Elaeis guineensis Jacq.) during 12 months of hermetic storage at 15°C with 10–12% moisture content (embryo moisture contents of 19–21%). The viability of both these and drier seeds was reduced greatly during this period at cooler storage temperatures of 0°C and −20°C, however. For example, intact seeds at 6.1–7.4% moisture content (embryo moisture contents of 9.1–12.0%, at which freezing damage would not be expected) lost viability more rapidly at 0°C and −20°C than at 15°C. Moreover, desiccation to 4–5% moisture content (4–6% embryo moisture content) reduced seed lot viability in some but not all cultivars. The results confirm earlier reports that oil palm is not recalcitrant, but neither is it orthodox. Thus, seed storage behaviour in oil palm appears to be intermediate between these categories. Additional results are presented which suggest that the seeds of the royal palm (Oreodoxa regia HBK) may also show intermediate seed storage behaviour.

Seed storage and germination studies of Garcinia talbotii Raizada ex Santapau seeds - an endemic tree from Western Ghats of India

2016 ◽  
Vol 23 (2) ◽  
pp. 73-78
Author(s):  
T. Sabu ◽  
P.S. Shameer ◽  
Chitra Rajeswary ◽  
N. Mohanan ◽  
C. Anilkumar
Keyword(s):  
Ex Situ Conservation ◽  
Storage Temperature ◽  
Seed Viability ◽  
Seed Storage ◽  
Ex Situ ◽  
Hermetic Storage ◽  
Western Ghats Of India ◽  
Relationship Of ◽  
The Relationship ◽  
And Storage

Seeds of Garcinia talbotii remained viable hardly for two weeks in open room conditions. As part of ex-situ conservation of Garcinia talbotii, seeds longevity was studied since seeds are the main propagule. For this, the relationship of seed viability with respect to different moisture content and storage temperature were analyzed. Seed storage behaviour is also investigated. Being recalcitrant, seeds are desiccation sensitive as well as chilling sensitive. During hermetic storage of seeds at 300C /70 % RH seeds retained viability about 6 months.

scholarly journals The impact of temperature patterns during storage of Scots pine and Norway spruce seeds on their germination and fungal infection rates

2021 ◽  
Vol 182 (1) ◽  
pp. 157-167
Author(s):  
M. A. Nikolaeva ◽  
E. Yu. Varentsova ◽  
G. F. Safina
Keyword(s):  
Seed Germination ◽  
Seed Viability ◽  
Seed Storage ◽  
Pathogenic Fungi ◽  
Forest Tree ◽  
Ex Situ ◽  
Seed Infection ◽  
Germination Capacity ◽  
Long Term Storage ◽  
The Impact

Relevance of the study. One of the ways to maintain the genetic diversity of forest-forming species is to preserve highquality seed material ex situ. However, the relationship between the diversity of pathogenic mycobiota and the duration and methods of forest tree seed storage remains underexplored. The results of research into this problem can be used in forest seed production and forest phytopathology.Materials and methods. For our study we used seeds ofРinus sylvestris L. and Picea abies (L.) Karst. of the orthodox type, harvested in the period of 1996–2011 and stored under different temperatures: +20°С, +4°С, –18°С, and in liquid nitrogen vapor (–182°С) since 2011. Prior to their storage, seed samples were dried to a moisture content of 4.2–4.4% and hermetically packed. Seed germination was tested before and after three, five and eight years of storage, following GOST 13056.6-97 standards. The level of seed infection and the composition of pathogenic fungi were assessed.Results. After eight years of storage at +20°С, pine and spruce seed germination capacity decreased by 13–60%, depending on the year of harvesting. Seed storage at –18°С and –182°С allowed us to prevent seed infection and preserve seed viability. In most cases, the germination energy and germination capacity were negatively correlated with the level of seed infection. The diversity of pathogenic (mold) fungi on the surface of seeds was represented by ten genera; the most common were saprotrophs: Aspergillus P. Micheli, Penicillium Link, Rhizopus Ehrenb., Scopulariopsis Bainier.Conclusion. At the present stage of research, the success of seed storage at low and ultralow temperatures was demonstrated. We recommend cryopreservation for the long-term storage of improved and valuable seeds.

scholarly journals Primed Lettuce Seeds Exhibit Increased Sensitivity to Moisture Content During Controlled Deterioration

HortScience ◽  
2007 ◽  
Vol 42 (6) ◽  
pp. 1436-1439 ◽  
Author(s):  
H.J. Hill ◽  
Jesse D. Cunningham ◽  
Kent J. Bradford ◽  
A.G. Taylor
Keyword(s):  
Moisture Content ◽  
Elevated Temperatures ◽  
Seed Viability ◽  
Seed Storage ◽  
Seed Priming ◽  
Storage Conditions ◽  
Seed Moisture Content ◽  
Moisture Contents ◽  
Lettuce Seeds ◽  
Rate Of Decline

The Ellis-Roberts seed viability equation is used to predict seed survival after storage at specified temperatures and moisture contents. Seed priming, which can break dormancy and accelerate germination, can also reduce seed storage life. Because primed seeds were not used in developing the Ellis-Roberts equation, the reciprocal nature of specific seed moisture content (MC, fresh weight basis) and temperatures that applies to nonprimed lettuce (Lactuca sativa L.) seeds may not apply to primed seeds. To determine how priming affects lettuce seeds in relation to the viability equation, an experiment was conducted using two cultivars, ‘Big Ben’ and ‘Parris Island Cos’. Seeds primed in polyethylene glycol 8000 (–1.45 MPa, 24 h at 15 °C) and nonprimed seeds were first adjusted to 6% and 9% moisture contents and then stored at 48 and 38 °C for up to 30 days, respectively. These storage conditions (6% MC and 48 °C; 9% MC and 38 °C) were predicted by the viability equation to result in equal longevities. Subsequent viability assays at 20 °C revealed that nonprimed seeds in both storage environments exhibited similar losses in viability over time, thus validating the Ellis-Roberts equation and the use of these conditions to apply different but equal aging stress. Primed seeds of both cultivars deteriorated faster than nonprimed seeds as expected. However, primed seeds did exhibit different rates of deterioration between the storage environments. Primed seeds stored at 9% MC and 38 °C deteriorated faster than primed seeds stored at 6% MC and 48 °C. The rate of decline in probit viability percentage was three times greater in primed ‘Big Ben’ seeds stored at 9% MC and 38 °C than for those stored at 6% MC and 48 °C (–1.34 versus –0.26 probits per day, respectively). ‘Parris Island Cos’ seeds stored at 9% MC and 38 °C had twice the rate of deterioration that those stored at 6% MC and 48 °C (–1.19 and –0.49 probits per day, respectively). The results indicate that primed lettuce seeds were more sensitive to the adverse effects of higher seed MC than were nonprimed seeds during storage at elevated temperatures.

Changes in seed quality during seed development and maturation in tomato

1992 ◽  
Vol 2 (2) ◽  
pp. 81-87 ◽  
Author(s):  
I. Demir ◽  
R. H. Ellis
Keyword(s):  
Lycopersicon Esculentum ◽  
Moisture Content ◽  
Developmental Stage ◽  
Seed Development ◽  
Seed Quality ◽  
Seed Viability ◽  
Dry Weight ◽  
Seed Filling ◽  
Moisture Contents ◽  
Seed Moisture

AbstractChanges in tomato (Lycopersicon esculentumMill.) seed quality were monitored during seed development and maturation in glasshouse experiments in 2 years. The end of the seedfilling period (mass maturity) occurred 35–41 d after anthesis (differing among trusses) in 1989 and 42 d after anthesis in 1990. Seed moisture contents at this developmental stage were 53–72% (wet basis), while the onset of ability to germinate (during 21-d tests at 20°/30°C) and the onset of tolerance to rapid enforced desiccation occurred just before (1990) or just after (1989) mass maturity. In 1989, seed quality was assessed primarily by seedling size in a glasshouse experiment; maximum mean seedling dry weight 25 d after sowing was not achieved until 24–40 d after mass maturity. In 1990, seed quality was assessed primarily by germination following storage; maximum normal germination after 35 d in storage at 40 °C with 14 ± 0.5% moisture content was attained 23 d after mass maturity, but with little difference among seed lots harvested 10 d earlier or up to 30 d later. The results contradict the hypothesis that maximum seed quality is attained at the end of the seed-filling period and that seed viability and vigour begin to decline immediately thereafter.

scholarly journals Effect of simulated flooding during rice seed development and maturation on subsequent seed quality

2018 ◽  
Vol 28 (1) ◽  
pp. 72-81 ◽  
Author(s):  
Sujittra Tejakhod ◽  
Richard H. Ellis
Keyword(s):  
Moisture Content ◽  
Seed Development ◽  
Seed Quality ◽  
Seed Viability ◽  
Dry Weight ◽  
Rice Seed ◽  
Hermetic Storage ◽  
Dry Storage ◽  
Sprouted Seeds ◽  
Harvest Maturity

AbstractThe resilience of seed quality in rice (Oryza sativaL.) to flooding was investigated. Pot-grown plants of thejaponicacv. Gleva, theindicacv. IR64, and the introgressed line IR64-Sub1 were submerged in water, to simulate flooding, for 3‒5 days at different stages of seed development and maturation. Mean seed weight, pre-harvest sprouting, ability to germinate, and subsequent longevity in air-dry storage were assessed. Whereas seed quality in both IR64 and IR64-Sub1 was resilient to submergence, in Gleva the longer the duration of submergence and the later in development when plants were submerged the greater the pre-harvest sprouting. Thousand seed dry weight was reduced more by submergence in Gleva than IR64 or IR64-Sub1. At harvest maturity, few pre-harvest sprouted seeds were able to germinate upon rehydration after desiccation to 11‒12% moisture content. Seed longevity of the non-sprouted seed fraction in air-dry hermetic storage (40°C, 15% moisture content) was not affected greatly by submergence, but longevity of thejaponicarice was less than that of theindicarices due to the former's steeper seed survival curves. Longevity of the twoindicarices was predicted well by the seed viability equation and previously published estimates of viability constants for rice. The greater dormancy of IR64 and IR64-Sub1, compared with Gleva, enhanced resilience to pre-harvest sprouting and reduced thousand seed dry weight from plant submergence. There was little or no effect of plant submergence on subsequent air-dry storage longevity of non-sprouted seeds in any genotype.

scholarly journals Pola Penurunan Viabilitas dan Pengembangan Metode Pendugaan Vigor Daya Simpan Benih Pepaya (Carica Papaya L.)

2017 ◽  
Vol 7 (3) ◽  
pp. 146
Author(s):  
Astryani Rosyad ◽  
M. Rahmad Suhartanto ◽  
Abdul Qadir
Keyword(s):  
Moisture Content ◽  
Aging Time ◽  
Seed Quality ◽  
Initial Moisture Content ◽  
Accelerated Aging ◽  
Storage Period ◽  
Seed Storage ◽  
Seed Vigor ◽  
Develop Model ◽  
Papaya Seed

<p>ABSTRACT<br />Information of seed quality during storage can be determined through the actual storage and storability vigor estimation. This study aimed at comparing effective accelerated aging method<br />between physical and chemical, and studying the seed deterioration during storage in ambient (T =28-30 0C, RH=75-78%) and AC (T =18-20 0C, RH =51-60%) condition with three levels of initial moisture content (8-10%, 10-12%, and 12-14%) for 20 weeks. The final objective of this research<br />was to develop model for storability vigor of papaya seed. Two experiments, accelerated aging and seed storage were conducted at Seed Laboratory, Department of Agronomy and Horticulture, Bogor Agricultural University from October 2015 to May 2016. A completely randomized design with nested factors and four replications was applied to both experiments. The results showed that physical accelerated aging using IPB 77-1 MMM machine was more effective than chemical accelerated aging using IPB 77-1 MM machine for papaya seed. The viability of seed stored in AC condition remained high until the end of the storage period, whereas it declined at 16 week storage period in the ambient condition. The viability of seed with initial moisture content of 12-14% declined faster than that of initial moisture content of 8-10% after 18 week storage periode. The model used to estimate the storability vigor of papaya seed accurately was the equation y = a + b expcx where y : storability vigor estimation, x : aging time and a,b,c : constant value. Simulation of storability vigor estimation with constant value of a, b, c and input of aging time can estimate storability seed vigor in actual storage.<br />Keywords: accelerated aging, IPB 77-1 MM machine, IPB 77-1 MMM machine, seed storage, simulation</p><p>ABSTRAK<br />Informasi mutu benih selama penyimpanan dapat diketahui melalui penyimpanan secara aktual dan pendugaan vigor daya simpan. Penelitian ini bertujuan untuk membandingkan metode<br />pengusangan cepat yang efektif antara fisik dengan kimia serta mempelajari pola penurunan viabilitas benih selama penyimpanan aktual pada kondisi simpan kamar (suhu =28-30 0C, RH =75-78%) dan AC (suhu =18-20 0C, RH =51-60%) dengan tiga tingkat kadar air awal (8-10%, 10-12%, dan 12-14%) selama 20 minggu. Tujuan akhirnya adalah membangun model vigor daya simpan benih pepaya. Penelitian pengusangan cepat dan penyimpanan dilakukan pada bulan Oktober 2015 sampai Mei 2016 di Laboratorium Benih, Departemen Agronomi dan Hortikultura, Institut Pertanian<br />Bogor. Kedua penelitian menggunakan rancangan acak lengkap tersarang dengan empat ulangan. Hasil penelitian menunjukkan bahwa pengusangan cepat secara fisik dengan alat IPB 77-1 MMM lebih efektif daripada pengusangan kimia dengan alat IPB 77-1 MM untuk benih pepaya. Viabilitas benih yang disimpan pada kondisi AC tetap tinggi hingga akhir periode simpan, sedangkan pada kondisi kamar penurunan viabilitas dimulai pada periode simpan 16 minggu. Benih yang disimpan dengan tingkat KA awal sebesar 12-14% lebih cepat mengalami penurunan viabilitas mulai periode simpan 18 minggu dibandingkan dengan benih dengan KA awal 8-10%. Hasil penelitian juga menunjukkan terdapat korelasi yang erat antara pola kemunduran benih pada pengusangan cepat dan penyimpanan aktual, sehingga model pendugaan vigor daya simpan (y) berdasarkan waktu pengusangan (x) dapat disusun dengan persamaan y = a + b expcx. Simulasi pendugaan vigor daya simpan dengan nilai konstanta a, b, dan c serta input waktu pengusangan dapat menduga vigor daya simpan benih selama penyimpanan aktual.<br />Kata kunci: alat IPB 77-1 MM, alat IPB 77-1 MMM, pengusangan cepat, penyimpanan benih,<br />simulasi</p>

Seed Longevity and Germination Behaviour in Eugenia roxburghii

2021 ◽  
Vol 27 (3) ◽  
pp. 167-171
Author(s):  
Shareef Muhammed ◽  
Chitra Rajeswary ◽  
Anil Chandran
Keyword(s):  
Storage Temperature ◽  
Seed Viability ◽  
Seed Longevity ◽  
Ex Situ ◽  
Hermetic Storage ◽  
Germination Behaviour ◽  
Garden Plant ◽  
Relationship Of ◽  
The Relationship ◽  
And Storage

Eugenia roxburghii is an evergreen graceful shrub with a tremendous potential as garden plant. As a part of ex-situ conservation and popularization of the species, seed longevity was studied by understanding the relationship of seed viability with respect to different moisture contents and storage temperature. Seeds are recognized as recalcitrant, being desiccation as well as chilling sensitive. During hermetic storage, seeds stored at 300C/70%RH retained viability for about 5 months and 4 months in 200C/20% RH. Seeds can be best stored for five months in laboratory conditions.

High seed viability recorded in an endangered endemic species, 'Isoplexis isabelliana' (Scrophulariaceae), after more than 30 years of storage in a conservation seed bank

2021 ◽  
Vol 42 ◽  
pp. e69341
Author(s):  
Miguel Ángel González Pérez ◽  
Nereida Cabrera-García ◽  
Isabel Cayon-Fernández
Keyword(s):  
Seed Bank ◽  
Germination Rate ◽  
Genetic Material ◽  
Natural Populations ◽  
Seed Viability ◽  
Seed Storage ◽  
Botanical Garden ◽  
Storage Conditions ◽  
Seed Banks ◽  
Ex Situ

Conservation seed banks are essential for ex-situ conservation of genetic biodiversity. These institutions are especially relevant for threatened species and play a vital role in their conservation by preserving genetic material. However, samples deposited in the seed banks must germinate when necessary to use them (i.e., recovery plans, etc.). This study uses four accessions of the endemic endangered species from Gran Canaria Island (Canary Islands), Isoplexis isabelliana (Webb & Berthel.) Masf. (Scrophulariaceae). Germination tests were carried out to measure seed viability through time and the possible impact of seed storage on their viability. These accessions have been kept in the seed bank for four months to thirty years under different storage conditions. Germination results differed for seeds after 45 days of exposition using 16 hours light and 8 hours darkness at 17 °C. Accessions kept in the seed bank, independently of storage, showed a high germination percentage (89%). Whereas the accessions with rough storage conditions showed a 0% germination rate. The results highlighted the good state of conservation of the material deposited in the Seed Bank of the Botanical Garden "Viera y Clavijo" and the reliability of the temperature and humidity conditions in which the seeds of I. isabelliana have been stored. We consider these results as momentous since several natural populations of I. isabelliana has been affected by the last forest fire on the island.

Reimposition of conditional dormancy during air-dry storage of prechilled Sitka spruce seeds

1998 ◽  
Vol 8 (2) ◽  
pp. 113-122 ◽  
Author(s):  
Steve K. Jones ◽  
Peter G. Gosling ◽  
Richard H. Ellis
Keyword(s):  
Moisture Content ◽  
Water Potential ◽  
Seed Storage ◽  
Sitka Spruce ◽  
Picea Sitchensis ◽  
Seed Moisture Content ◽  
Moisture Contents ◽  
Dry Storage ◽  
Low Water Potential ◽  
Negative Exponential

AbstractPrechilling seeds of Sitka spruce (Picea sitchensis[Bong.] Carr.) at 4°C with 30% moisture content for 12–14 weeks (84–98 d) removed conditional dormancy (i.e. they were then able to germinate at 10°C). The non-dormant status was preserved after redrying to 6% moisture content. However, conditional dormancy was gradually reimposed during subsequent air-dry storage at 4°C and 6% seed moisture content in all five seed lots tested. Further investigations with one seed lot showed that reimposition was reversed by a second prechill treatment, but was reimposed again during subsequent air-dry storage. The trend of dormancy reimposition within seed lots over time was quantified by negative exponential relations between ability to germinate at 10°C and duration of air-dry storage. The progress of dormancy reimposition was influenced by seed storage moisture content and was most rapid at 4–10%. At higher moisture contents (15 and 20%) the rate of the reimposition of conditional dormancy was much reduced, while at moisture contents of 25 and 30% further loss in dormancy occurred. Thus it is clear that dormancy reimposition occurred during storage at low water potential rather than solely during desiccation from high to low water potential.

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