scholarly journals Reserve mobilization during imbibition of stored Gliricidia sepium (Jacq.) Steud. (Leguminosae - Papilionoideae) seeds

2011 ◽  
Vol 33 (3) ◽  
pp. 549-560
Author(s):  
Renata Conduru Ribeiro Reis ◽  
Bárbara França Dantas ◽  
Renato Delmondez de Castro ◽  
Cimille Gabrielle Cardoso Antunes ◽  
Fabrício Francisco Santos da Silva ◽  
...  
Keyword(s):  
Soluble Sugars ◽  
Seed Viability ◽  
Storage Period ◽  
Seed Storage ◽  
Gliricidia Sepium ◽  
Storage Conditions ◽  
Ambient Conditions ◽  
Total Soluble Sugars ◽  
Drought Tolerant ◽  
Semi Arid Region

Gliricidia sepium is a drought-tolerant species, easily multiplied by seeds, and has been exploited by farmers as a source of forage in the semi-arid region of northeast Brazil. The objective of the present study was to evaluate the effect of seed storage on the mobilization of reserves during imbibition of "Gliricidia" seeds. Freshly-harvested seeds were packed in kraft paper bags and stored for three and six months in the laboratory under ambient conditions (25 º C ± 3 T and 75% ± 3 RH). Cotyledons were isolated from imbibed seeds and macerated for the extraction and quantification of total soluble sugars, reducing sugars, sucrose and starch, as well as of proteins, amino acids and for amylase activity. Storage under these conditions resulted in an increase in seed water content although germination remained at relatively high levels (86%). Seed macromolecule levels showed significant variation with the storage period and imbibition and these variations were associated with a loss in seed viability due to inadequate storage conditions.

scholarly journals Physiological quality of Gliricidia sepium (Jacq.) Steud. (Leguminosae - Papilionoideae) seeds subjected to different storage conditions

2012 ◽  
Vol 36 (2) ◽  
pp. 229-235 ◽  
Author(s):  
Renata Conduru Ribeiro Reis ◽  
Claudinéia Regina Pelacani ◽  
Cimille Gabrielle Cardoso Antunes ◽  
Bárbara França Dantas ◽  
Renato Delmondez de Castro
Keyword(s):  
Water Content ◽  
Seed Viability ◽  
Gliricidia Sepium ◽  
Storage Conditions ◽  
Physiological Processes ◽  
Plastic Bags ◽  
Starting Point ◽  
Cultivated Plant ◽  
Physiological Quality

Studies of seed physiological processes represent the starting point for the sustainable utilization of native or cultivated plant species from the caatinga biome for which germination studies are still scarce. In order to determine adequate methods for the conservation of Gliricidia sepium seeds, the physiological quality of propagules exposed to different conditions and times of storage were examined. Recently collected seeds of G. sepium were analyzed immediately, while the other sample was stored for three, six, nine, or twelve months in plastic or paper bags under refrigeration or at room temperature for further analysis. The germination rates of seeds from each storage regime were determined and these rates compared to their water content. It was observed that germination capacity was directly related to changes in seed humidity. Storing seeds in plastic bags yielded the best conservation, mainly when they were stored at low temperatures. However, even at temperatures near 25°C it was possible to prolong seed viability when their water content was controlled through the use of impermeable packing.

scholarly journals Biological features of seeds of a wild-growing species of Poterium polygamum

2022 ◽  
Vol 354 (11-12) ◽  
pp. 97-99
Author(s):  
M. A. Starostina ◽  
N. G. Lapenko
Keyword(s):  
Natural Population ◽  
Seed Viability ◽  
Storage Period ◽  
Seed Storage ◽  
Scientific Basis ◽  
The Third ◽  
Biological Features ◽  
High Germination ◽  
Grass Stand ◽  
The Subject

Relevance. Biological features (morphometric indicators, viability and germinating power) of Poterium polygamum seeds found in natural communites of the Stavropol Territory have been studied. The conservation of wild-growing plant species in the natural population and the rational use of their potential are relevant.Methods. Years of study — 2013–2021. The subject of the study is Poterium polygamum seeds. Seeds were collected in 2013. Studies were carried out according to the methods of studying seed material. Freshly harvested seeds and seeds of different shelf life were germinated in petri dishes at a temperature of 18–20 °C.Results. The collected seeds of Poterium polygamum are quite large. The length of the seeds is 4–5 mm, the width is about 2.5 mm. The mass of 1000 pieces of seeds on average was 10.59 grams. Complete absence of viability of freshly harvested Poterium polygamum seeds was revealed. After three months, seed viability was 97%. This is a shallow physiological rest associated with the post-harvest maturation period. During the storage of seeds, their high germination was established — from 70 to 100%. The duration of seed storage is 8 years. The largest number of germinated seeds and their germinating power were noted in the third year of their storage (100%). Seeds germinated on the third or fourth day. The differences between the viability of seeds and their germinating power are not great. In the eighth year of storage of Poterium polygamum seeds, viability and germinating power were slightly reduced. The storage period of seeds, in which they retain the ability to germinate and the germinating power, is long. It has been found that the wild species Poterium polygamum belongs to plants with a high (70 to 100%) viability. This species is regularly resumed in the natural population. The revealed biological features of Poterium polygamum seeds can become the scientific basis during the reconstruction of degraded natural grass stand and in selection work.

High seed viability recorded in an endangered endemic species, 'Isoplexis isabelliana' (Scrophulariaceae), after more than 30 years of storage in a conservation seed bank

2021 ◽  
Vol 42 ◽  
pp. e69341
Author(s):  
Miguel Ángel González Pérez ◽  
Nereida Cabrera-García ◽  
Isabel Cayon-Fernández
Keyword(s):  
Seed Bank ◽  
Germination Rate ◽  
Genetic Material ◽  
Natural Populations ◽  
Seed Viability ◽  
Seed Storage ◽  
Botanical Garden ◽  
Storage Conditions ◽  
Seed Banks ◽  
Ex Situ

Conservation seed banks are essential for ex-situ conservation of genetic biodiversity. These institutions are especially relevant for threatened species and play a vital role in their conservation by preserving genetic material. However, samples deposited in the seed banks must germinate when necessary to use them (i.e., recovery plans, etc.). This study uses four accessions of the endemic endangered species from Gran Canaria Island (Canary Islands), Isoplexis isabelliana (Webb & Berthel.) Masf. (Scrophulariaceae). Germination tests were carried out to measure seed viability through time and the possible impact of seed storage on their viability. These accessions have been kept in the seed bank for four months to thirty years under different storage conditions. Germination results differed for seeds after 45 days of exposition using 16 hours light and 8 hours darkness at 17 °C. Accessions kept in the seed bank, independently of storage, showed a high germination percentage (89%). Whereas the accessions with rough storage conditions showed a 0% germination rate. The results highlighted the good state of conservation of the material deposited in the Seed Bank of the Botanical Garden "Viera y Clavijo" and the reliability of the temperature and humidity conditions in which the seeds of I. isabelliana have been stored. We consider these results as momentous since several natural populations of I. isabelliana has been affected by the last forest fire on the island.

scholarly journals Primed Lettuce Seeds Exhibit Increased Sensitivity to Moisture Content During Controlled Deterioration

HortScience ◽  
2007 ◽  
Vol 42 (6) ◽  
pp. 1436-1439 ◽  
Author(s):  
H.J. Hill ◽  
Jesse D. Cunningham ◽  
Kent J. Bradford ◽  
A.G. Taylor
Keyword(s):  
Moisture Content ◽  
Elevated Temperatures ◽  
Seed Viability ◽  
Seed Storage ◽  
Seed Priming ◽  
Storage Conditions ◽  
Seed Moisture Content ◽  
Moisture Contents ◽  
Lettuce Seeds ◽  
Rate Of Decline

The Ellis-Roberts seed viability equation is used to predict seed survival after storage at specified temperatures and moisture contents. Seed priming, which can break dormancy and accelerate germination, can also reduce seed storage life. Because primed seeds were not used in developing the Ellis-Roberts equation, the reciprocal nature of specific seed moisture content (MC, fresh weight basis) and temperatures that applies to nonprimed lettuce (Lactuca sativa L.) seeds may not apply to primed seeds. To determine how priming affects lettuce seeds in relation to the viability equation, an experiment was conducted using two cultivars, ‘Big Ben’ and ‘Parris Island Cos’. Seeds primed in polyethylene glycol 8000 (–1.45 MPa, 24 h at 15 °C) and nonprimed seeds were first adjusted to 6% and 9% moisture contents and then stored at 48 and 38 °C for up to 30 days, respectively. These storage conditions (6% MC and 48 °C; 9% MC and 38 °C) were predicted by the viability equation to result in equal longevities. Subsequent viability assays at 20 °C revealed that nonprimed seeds in both storage environments exhibited similar losses in viability over time, thus validating the Ellis-Roberts equation and the use of these conditions to apply different but equal aging stress. Primed seeds of both cultivars deteriorated faster than nonprimed seeds as expected. However, primed seeds did exhibit different rates of deterioration between the storage environments. Primed seeds stored at 9% MC and 38 °C deteriorated faster than primed seeds stored at 6% MC and 48 °C. The rate of decline in probit viability percentage was three times greater in primed ‘Big Ben’ seeds stored at 9% MC and 38 °C than for those stored at 6% MC and 48 °C (–1.34 versus –0.26 probits per day, respectively). ‘Parris Island Cos’ seeds stored at 9% MC and 38 °C had twice the rate of deterioration that those stored at 6% MC and 48 °C (–1.19 and –0.49 probits per day, respectively). The results indicate that primed lettuce seeds were more sensitive to the adverse effects of higher seed MC than were nonprimed seeds during storage at elevated temperatures.

scholarly journals Effect of Packaging and Storage on Quality Characteristics of Dried Wild Pomegranate Arils (Anardana) Prepared in Solar Tunnel Drier

2020 ◽  
pp. 8-23
Author(s):  
Abhimanyu Thakur ◽  
N. S. Thakur ◽  
Hamid . ◽  
Pradeep Kumar ◽  
Kanchan Bhatt
Keyword(s):  
Value Added ◽  
Storage Period ◽  
Titratable Acidity ◽  
Punica Granatum ◽  
Storage Condition ◽  
Storage Conditions ◽  
Quality Characteristics ◽  
Ambient Conditions ◽  
Hydroxymethyl Furfural ◽  
And Storage

Wild pomegranate (Punica granatum L.) fruits were procured from Karsog area of Mandi district of Himachal Pradesh, India (1265 m above mean sea level). The arils extracted from the fruits were dehydrated in a solar tunnel (30-45°C) drier to prepare value added product Anardana. The developed product was packed in three different packaging materials viz. gunny bags, aluminium laminated pouches (ALP) and aluminium laminated pouches with vacuum (ALPV) and stored under ambient and refrigerated temperature for a period of 12 months so as to evaluate overall effect of storage period, storage condition and packaging material. After 12 months of storage period, higher retention of various quality characteristics like TSS, titratable acidity, ascorbic acid, total sugars, anthocyanins content, sensory characteristic scores with less increase in moisture content, NEB (non-enzymatic browning),HMF (hydroxymethyl furfural) and furfural content was observed in Anardana packed in ALPV followed by ALP and gunny bags. The changes in the quality characteristics of the Anardana were slower in refrigerated storage conditions as compared to ambient conditions.

scholarly journals Studies on Storage Quality Evaluation of Dried Wild Pomegranate Arils (Anardana) Prepared in Mechanical Cabinet Drier

2020 ◽  
pp. 79-91
Author(s):  
Abhimanyu Thakur ◽  
N. S. Thakur ◽  
.` Hamid ◽  
Pradeep Kumar ◽  
Sunakshi Gautam
Keyword(s):  
Acid Content ◽  
Storage Period ◽  
Storage Conditions ◽  
Quality Characteristics ◽  
Himachal Pradesh ◽  
Soluble Solids ◽  
Appreciable Amount ◽  
Refrigerated Storage ◽  
Ambient Conditions ◽  
Jammu And Kashmir

Wild pomegranate (Punica granatum L.), fruit is widely found in hilly slopes of Himachal Pradesh, Uttarakhand and Jammu and Kashmir. It contains higher amount of acid content along with other quality characteristics. To exploit the appreciable amount of acid content in this fruit, a popular dried product known as anardana was prepared in mechanical cabinet drier from the fruits procured from Karsog area of Mandi district of Himachal Pradesh, India (1265 m above mean sea level). The anardana was packed in aluminium laminated pouch with vacuum (ALPV), aluminium laminated pouch (ALP) and gunny bags which were stored under ambient and refrigerated storage conditions so as to study the effect of packaging and storage on its quality. During storage, moisture, water activity, reducing sugars, NEB (Non enzymatic browning), HMF (Hydroxymethyl furfural) and furfural content increased whereas, TSS (Total soluble solids), titratable acidity, total sugars, ascorbic acid, anthocyanins, starch, total fibre and residual SO2 decreased during storage. After 12 months of storage period, higher retention of various quality characteristics was observed in anardana packed in ALPV followed by ALP and gunny bags. However, changes were slower in refrigerated storage conditions as compared to that under ambient conditions.

scholarly journals Seed quality of Amburana cearensis (Allemão) A.C. Sm. (Fabaceae) is influenced by storage condition

2017 ◽  
Vol 39 (4) ◽  
pp. 401-409 ◽  
Author(s):  
Marcelo do Nascimento Araujo ◽  
Marisol Ferraz ◽  
Fabiana Karla Araújo Américo ◽  
Fabrício Francisco Santos Silva ◽  
Bárbara França Dantas ◽  
...  
Keyword(s):  
Low Temperatures ◽  
Reducing Sugars ◽  
Seed Quality ◽  
Germination Rate ◽  
Seedling Emergence ◽  
Soluble Sugars ◽  
Storage Condition ◽  
Storage Conditions ◽  
Total Soluble Sugars

Abstract: The aim of this study was to evaluate the effects of storage conditions on the germination of A. cearensis seeds. The experimental design was completely randomized into split plots over time with four replicates. The storage conditions of the airtight containers in the refrigerator and laboratory, paper bags in the laboratory and liquid nitrogen were assessed for 27 months. In the laboratory, we evaluated the germination, the germination rate, uniformity of germination, and total soluble and reducing sugars in the radicle. In the greenhouse, we evaluated seedling emergence, emergence rate and height of 30-day-old seedlings. Seeds stored in the refrigerator maintained a high initial germination rate, which decreased from the 21st month. Seeds stored in paper bags in the laboratory showed low emergence and small seedlings. Total soluble sugars and reducing sugars were mobilized when the seeds were stored at low temperatures. Thus, it is not advisable to store A. cearensis seeds in a laboratory environment without airtight containers. A. cearensis seeds kept in a refrigerated environment maintained their viability for at least two years.

CHANGES IN WATER AND CARBOHYDRATE STATUS OF THREE NURSERY SPECIES IN CONTAINERS DURING OVERWINTER STORAGE IN THREE ENVIRONMENTS

1979 ◽  
Vol 59 (3) ◽  
pp. 747-755 ◽  
Author(s):  
CALVIN CHONG ◽  
R. L. DESJARDINS ◽  
C. S. LIN
Keyword(s):  
Starch Content ◽  
Water Status ◽  
Soluble Sugars ◽  
Single Layer ◽  
Storage Period ◽  
Storage Conditions ◽  
Clear Cut ◽  
Picea Pungens ◽  
Juniperus Sabina ◽  
Carbohydrate Status

During overwinter storage in an open coldframe, a shaded single-layer polyhouse, and an unshaded double-layer polyhouse, changes in water potentials, moisture content, soluble sugars, and starch were compared in shoots of Colorado spruce (Picea pungens Egnelm.) and Ramlosa juniper (Juniperus sabina L. ’Ramlosa’), both container-grown for one season, and also of globe cedar (Thuja occidentalis L. ’Woodwardii’), field-potted 1 mo prior to testing. Higher water status and higher sugar and starch content were observed in all species in the polyhouse than in the coldframe. Differences between effects of the polyhouses were less clear-cut. During the storage period, higher water status and higher soluble sugars were generally observed in spruce than in juniper and cedar. The sugar to starch ratio (an index of metabolic activity) remained relatively constant for juniper and cedar but decreased during the period of storage for spruce. Extent of winter injury, as indicated by post-winter storage conditions of plants, was 10 and 40% less for cedar and juniper, respectively, overwintered in the polyhouses than in the coldframe; spruce survived equally well in all three environments.

scholarly journals Screening for drought tolerance in cultivars of the ornamental genusTagetes(Asteraceae)

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2133 ◽  
Author(s):  
Raluca Cicevan ◽  
Mohamad Al Hassan ◽  
Adriana F. Sestras ◽  
Jaime Prohens ◽  
Oscar Vicente ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Drought Tolerance ◽  
Plant Growth ◽  
Growth Parameters ◽  
Soluble Sugars ◽  
Stem Length ◽  
Total Phenolic ◽  
Total Soluble Sugars ◽  
Drought Tolerant ◽  
Components Analysis

Drought tolerance was evaluated in twelve cultivars of three ornamentalTagetesspecies (T. patula,T. tenuifoliaandT. erecta). A stress treatment was performed by completely stopping watering of plants maintained in controlled greenhouse conditions. After three weeks, several plant growth parameters (stem length (SL), fresh weight (FW) and water content (WC)), photosynthetic pigments (chlorophylls and carotenoids (Car)), osmolytes (proline (Pro), glycine betaine (GB) and total soluble sugars (TSS)), an oxidative stress maker (malondialdehyde (MDA)) and antioxidants (total phenolic compounds (TPC) and total flavonoids (TF)) were measured. Considerable differences in the evaluated traits were found among the control and drought-stressed plants. Drought stress generally caused a marked reduction in plant growth and carotenoid pigments, and an increase in soluble solutes and oxidative stress. For most cultivars, proline levels in stressed plants increased between 30 and 70-fold compared to the corresponding controls. According to the different measured parameters, on averageT. erectaproved to be more tolerant to drought thanT. patulaandT. tenuifolia. However, a considerable variation in the tolerance to drought was found within each species. The traits with greater association to drought tolerance as well as the most tolerant cultivars could be clearly identified in a principal components analysis (PCA). Overall, our results indicate that drought tolerant cultivars ofTagetescan be identified at early stages using a combination of plant growth and biochemical markers.

scholarly journals Model Dinamik Vigor Daya Simpan Benih Kedelai pada Penyimpanan Terbuka

2016 ◽  
Vol 34 (3) ◽  
pp. 219
Author(s):  
Ari Wahyuni ◽  
M. R. Suhartanto ◽  
Abdul Qadir
Keyword(s):  
Moisture Content ◽  
Initial Moisture Content ◽  
Seed Viability ◽  
Soybean Seed ◽  
Storage Period ◽  
Seed Storage ◽  
Seed Moisture Content ◽  
Seed Moisture ◽  
Input Model ◽  
Seed Storability

<p>Soybean seed viability declines during seed storage. Soybean seed deteriorates rapidly, affected by its high protein content and often high humidity in the tropical environment. This research was aimed to develop dynamic model of soybean seed viability in an open storage. The study was conducted in three stages, namely: 1) desk study, 2) seed storage experiment, 3) development of seed storage model, simulation and verification of the model. The second stage of the experiment consisted of soybean seed storing and germination testing using completely randomized design. Treatments were three initial moisture content (7-8%, 9-10% and 11-12%) and four varieties of soybean (Anjasmoro, Wilis, Detam-1 and Detam-2). The results showed that the seed behaviour during storage period were affected by initial seed moisture content, initial viability, varieties and environmental condition. Therefore, seed moisture content, initial viability and varieties may be used as input model. Moisture content, integreting seed respiration, electric conductivity and seed storability vigor (VDSDB) were as model output. Simulation of Seed Storability Vigor Prediction Model with Model Construction Layer-Stella (MCLS) using relative humidity (RH), temperature, seed permeability, initial moisture content and initial viability as input model could logically predict the seed moisture content and seed storability vigor (VDSDB).</p>

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