scholarly journals Ecotoxicity assessment of camphor oxime using Microtox assay – preliminary research

2018 ◽  
Vol 44 ◽  
pp. 00066 ◽  
Author(s):  
Ewa Kielka ◽  
Agata Siedlecka ◽  
Mirela Wolf ◽  
Sylwia Stróżak ◽  
Katarzyna Piekarska ◽  
...  

The ecotoxicity assessment of new substances is crucial for their considered launch on the market. Due to various properties of chemical compounds, however, the application of biotests can be challenging. In the case of insoluble compounds, a proper, non-toxic solvent must be applied. The presented preliminary results demonstrate the extremely high ecotoxicity of camphor and camphor oxime, assessed by means of the Microtox system. A 81.9% Basic Test with 14 dilutions was applied. The ecotoxicity of a solvent (i.e. DMSO) was also assessed. The initial concentration of substances was 5%. EC50 values reached 0.00023% and 0.00007% for camphor and camphor oxime, respectively, after 15 min. of incubation of the samples with bioluminescent bacteria. The ecotoxicity of the solvent remained negligible. Due to the extremely high ecotoxicity values of the tested substances, another range of concentrations needs to be applied in future tests to confirm the results.

2002 ◽  
Vol 207 ◽  
pp. 309-311
Author(s):  
G. L. H. Harris ◽  
D. Geisler ◽  
W. E. Harris ◽  
J. E. Hesser

We have obtained CMR photometry for a roughly 1° square region centered on NGC 5128. Preliminary results indicate that the limiting magnitude of the images is ≳ 1 magnitude fainter than the peak of the globular cluster luminosity function (GCLF) at R ⋍ 21.


2016 ◽  
Vol 70 (10) ◽  
pp. 1739-1750
Author(s):  
Carina Svendsen ◽  
Thomas Skov ◽  
Frans W.J. van den Berg

Fluorescence spectroscopy is a sensitive and selective technique, which can be of great value in bioprocesses to provide online, real-time measures of chemical compounds. Although fluorescence spectroscopy is a widely studied method, not much attention has been given to issues concerning intensity variations in the fluorescence landscapes due to pH fluctuations. This study elucidates how pH fluctuations cause intensity changes in fluorescence measurements and thereby decreases the quality of the subsequent quantification. A photo-degradation process of riboflavin was investigated using fluorescence spectroscopy and used as a model system. A two-step modeling approach, combining weighted PARAllel FACtor analysis (PARAFAC) with weighted nonlinear regression of the known reaction kinetics, is suggested as a way of handling the fluorescence intensity shifts caused by the pH changes. The suggested strategy makes it possible to compensate for uncertainties in the shifted data and thereby obtain more reliable concentration profiles for the chemical compounds and kinetic parameters of the reaction.


2011 ◽  
Vol 56 (4) ◽  
pp. 1177-1183
Author(s):  
P. Falkowski ◽  
P. Elert

Application of Water-Thinnable Photopolymerizable Resin for Shaping of Microreactors - Preliminary ResultsThe article presents the preliminary research on application of synthesized water-thinnable polyester resin for shaping of ceramic element by photopolymerization. The ceramic pastes made of alumina (30 vol%) and photocurable resins were used to prepare thin layers (tapes) and then were exposed to UV radiations. The cure depth and König hardness of cured layers were determined as well as the effect of a photoinitiator concentration on polymerization. The density of sintered ceramic samples also were examined. The research showed that the developed pastes with water-thinnable resin might be used to mold the ceramic elements by soft lithography method. The results obtained for polyester resin were compared with results obtained for commercially available photocurable resin.


2017 ◽  
Vol 6 (1) ◽  
pp. 79
Author(s):  
M Haviz

Abstract The purpose of this research was to design and develop a new model of education surau and madrasah minangkabau (SMM) Indonesia. The method used is educational design research with phase: preliminary research, prototyping phase and assessment phase. The participants of this research are 120 social religious activists as practitioner and 3 experts. The instrument used is validation and observation sheets. Means and standard deviation are calcualted for finding the level of validity and observation of implementation of prototype. The result show that the characteristics of prototype are organization, minangkabau knowledge, pedagogical competencies and social awareness. At process of design, a new model education SMM develop based on the as reality and good practical education. At construction of model, a new model education SMM is constructed by spesific program. At modernization of the education system, a new model education SMM must concist Islamic and indigenes Minangkabau value. At flexible pedagogies and intervention, a new model education SMM require new pedagogical ideas or themes and a special intervention from Islamic universities and stakeholders. In conclusion, design and develop a new model SMM Indonesia have the relevancy and internal consistency to stop the "stationary phase or run slowly" of surau and madrasah. Keywords: New Model Of Education, Surau And Madrasah Minangkabau (SMM) Abstrak Penelitian ini bertujuan untuk merancang dan mengembangkan model pendidikan baru surau dan madrasah minangkabau (SMM) Indonesia. Metode yang digunakan adalah penelitian pengembangan, yang terdiri dari 3 fase yaitu preliminary, prototype dan assessment. Partisipan penelitian berjumlah 120 orang aktivis sosial religius sebagai praktisi dan 3 orang ekspert (ahli) Instrumen yang digunakan adalah lembar validai dan observasi. Penghitungan nilai rata-rata dan standar deviasi dilakukan untuk menentukan tingkat validitas dan implementasi prototipe. Hasil penelitian memperlihatkan bahwa karakteristik prototipe penelitian adalah organisasi, pengetahuan adat minangkabau, kompetensi pedagogik dan social awareness. Pada aspek proses perancangan, model baru pendidikan SMM dikembangkan berdasarkan realitas dan prkatik pendidikan yang baik. Pada aspek konstruksi, model pendidikan SMM memuat nilai keislaman dan adat minangkabau. Pada aspek pedagogik dan intrervensi, model baru pendidikan SMM memerlukan pedagogik dengan tema baru, dan intervensi yang khusus dari Perguruan Tinggi Keagamaan Islam dan stakeholder. Sehingga disimpulkan, perancangan dan pengembangan pendidikan model baru SMM Indonesia telah memiliki kriteria relevansi dan konsistensi internal dalam penelitian pengembangan.   Kata Kunci: Model Pendidikan Baru, Surau And Madrasah Minangkabau (SMM)


PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12442
Author(s):  
Kristina Klein ◽  
Sebastian Heß ◽  
Ulrike Schulte-Oehlmann ◽  
Jörg Oehlmann

Weathering of plastics leads to the formation of increasingly smaller particles with the release of chemical compounds. The latter occurs with currently unknown environmental impacts. Leachate-induced effects of weathered microplastics (MPs) are therefore of increasing concern. To investigate the toxicity of the chemical mixtures from such plastics, we exposed the freshwater shrimp Neocaridina palmata to enriched leachates from unweathered and artificially weathered (UV-A/B light) MPs (≤1 mm) from recycled low-density polyethylene (LDPE-R) pellets and from a biodegradable, not fully bio-based starch blend (SB) foil. We analyzed the individual locomotor activity (moved distance and frozen events) on day 1, 3, 7 and 14 of exposure to five leachate concentrations equivalent to 0.40–15.6 g MPs L−1, representing the upper scale of MPs that have been found in the environment. The median moved distance did not change as a function of concentration, except for the unweathered SB treatment on day 14 that indicated hyperactivity with increasing concentrations. Significant impacts were solely detected for few concentrations and exposure days. Generally, no consistent trend was observed across the experiments. We further assessed the baseline toxicity of the samples in the Microtox assay and detected high bioluminescence inhibitions of the bacterium Aliivibrio fischeri. This study demonstrates that neither the recycled nor the biodegradable material are without impacts on test parameters and therefore cannot be seen as safe alternative for conventional plastics regarding the toxicity. However, the observed in vitro toxicity did not result in substantial effects on the behavior of shrimps. Overall, we assume that the two endpoints examined in the atyid shrimp N. palmata were not sensitive to chemicals leaching from plastics or that effects on the in vivo level affect other toxic endpoints which were not considered in this study.


Author(s):  
Manuela Ingaldi

There are many service enterprises on the market, which offers different type of services practically every day. Quality assessment of such services is very difficult. There are many methods that can be used in such a situation, but it should be emphasized that in such researches customers’ opinions are usually used. The purpose of the paper is to present a survey on the use of methods of service quality assessment. In the paper preliminary results of the survey from point of view of the service enterprises are presented. The enterprises were asked to indicate what methods they used, what information they received thanks to such research, what happened to the obtained results and what problems they en-countered. Preliminary research was carried out between September and December 2017. The re-search will be continued throughout 2018. At the same time, the research is conducted from the point of view of the customers of such enterprises. The final results of the research will help the service en-terprises to choose right research method and can be used by various service companies.


Author(s):  
A. Franklin ◽  
G. D. Pickett ◽  
N. A. Holme ◽  
R. L. Barrett

In England the fisheries for both scallops (Pecten maximus) and queens (Chlamys opercularis) expanded rapidly in the 1970s. Considerable research has been undertaken to develop an effective method of surveying stocks of these species in order to assess the impact of this greatly increased fishing effort on commercial populations. Visual photographic surveys seemed likely to provide the best means of assessing populations (Pickett & Franklin, 1975) and this paper outlines the preliminary results obtained from such a system - a towed sledge carrying both television and still cameras, developed at the Plymouth Laboratory.


2018 ◽  
Vol 558 (3) ◽  
pp. 20-23
Author(s):  
Katarzyna Hildt-Ciupińska

Self-assessment of health is an important factor determining caring for health, expressed by health behaviours. Lifestyle and health behaviours play a key role in shaping health as well as the risk of many diseases and mortality. This article presents preliminary results of research on self-assessment of health and caring for health among men active on the labour market. A total of 130 men were surveyed with a questionnaire developed for this project. Men rated high their health and caring for health, and defined these concepts correctly. They had substantial knowledge on health and caring for health. However, this was theoretical knowledge only. It is necessary to examine their health behaviours.


2019 ◽  
Vol 100 ◽  
pp. 00036
Author(s):  
Wojciech Kołodyński ◽  
Katarzyna Piekarska ◽  
Daniel Strub

The bioluminescence inhibition bioassay using marine bacteria Vibrio fischeri is widely used as a tool to assess the toxicity of chemical compounds, because of the many benefits comprising cost and time effectiveness, rapidness, sensitivity, and ease of operation. In addition, the test is used for various types of organic and inorganic compounds. Due to the insolubility of organic compounds in water, a suitable solubilizer should be applied. The ecotoxicity of the solvent should be negligible in relation to marine bacteria. On account of superior human activities the synthesis of new chemical substances is inextricably linked to the emergence of chemical compounds that are a potential threat on environment. A Microtox system utilizing the 81.9% Basic Test with 14 dilutions was applied to pre-evaluate the ecotoxicity of β-cyclocitral oxime, citronellal oxime and perillaldehyde oxime. Substances solutions with an initial concentration of 0.036% in DMSO were prepared. The preliminary results show that the studied scent compounds are characterized by quite high toxicity. In order to confirm the received ecotoxicity values, it is necessary to carry out additional surveys using another range of concentrations and biotests on organisms at each trophic level.


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