scholarly journals The Resistance of Potatoes by Application of Trichoderma viride Antagonists Fungus

2018 ◽  
Vol 73 ◽  
pp. 06014
Author(s):  
Purwantisari Susiana ◽  
Priyatmojo Achmadi ◽  
P. Sancayaningsih Retno ◽  
S. Kasiamdari Rina ◽  
Budihardjo Kadarwati

Leaf blight disease caused by pathogenic fungus Phytophthora infestans is the major disease in potato that can reduce its production up to 100%. The use of biological agent Trichoderma viride as an inducing potato resistance against leaf blight disease has been considered potential method. The purpose of this study was to evaluate the use of biological agent Trichoderma viride in inducing potato plant resistance. The parameters observed were the growth of the potato plant and leaf blight intensity. Experimental research with complete randomized design with 6 treatments was applied. The results showed that the application of Trichoderma viride could reduce the intensity of leaf blight disease and increase the growth of the potato plant. Trichoderma viride application could improve the systemic resistance of potato plants.

2019 ◽  
Vol 6 (1) ◽  
pp. 27
Author(s):  
Asrul Asrul ◽  
Umrah Umrah

Bacterial leaf blight disease caused by Pantoea ananatis has been reported affects some species of Allium spp. Here, we determined the species of Allium spp. that are not the hosts of the P.ananatis through a host range test. The study designed as a completely randomized design with five replications. Pathogenic bacteria of P. ananatis inoculated to shallot, onion, spring onion, garlic, chives, leek, and cung spring onion in the greenhouse of the Faculty of Agriculture Tadulako University. The incubation period and symptoms of after inoculated by P. ananatis then observed. The results showed that four llium species (onion, onion, scallion, and garlic) were hosts of                P. ananatis while the other three species (chives, leek, and cung spring onion) were not hosts of          P. ananatis.


Author(s):  
Mandah Cicelia Takor ◽  
Ekwa Yawa Monono ◽  
Ojong Agbor Ntane ◽  
Jemimah Evenye Ngale ◽  
Lum A. Fontem

Taro leaf blight (TLB), a major disease of taro plant caused by Phytophthora colocasiae, a pseudo-fungus, that occurs in many taro producing areas, especially in the South West of Cameroon. A Survey was conducted to assess farmers’ knowledge, as well as to determine the incidence and the severity of the disease in taro fields. Structured questionnaires were administered in selected locations (Bokova, Ekona, Muea, Mile 16, Mutengene, and Likomba) of taro production. Two farms were selected with two quadrats of 64 m2 each were carved to assess disease incidence and severity on taro. The number of plants infected with taro leaf blight were counted and the area of the affected leaves measured was used to evaluate the disease incidence and disease severity. Data collected were subjected to ANOVA for complete randomized design and the means were compared using Tukey test at 0.05 probability levels. The results revealed that most of the farmers could identify the disease while practicing cultural methods in controlling the disease. The results also revealed significant differences (p = 0.001) in disease incidence and disease severity in all the localities, with Mile 16 showing the highest percentage mean of 96.53% and 85.59%, respectively. The severity scored showed high infection range of 3 – 3.95 in all locations except in Likomba (2.60).  It could be concluded from the results that there were high prevalence of taro leaf blight disease in Fako Division. Good management strategies are therefore required to control Taro leaf blight and improve taro production in the area.


Author(s):  
Anna Fitriana ◽  
Lukman Hakim ◽  
Marlina Marlina

Potato leaf blight is caused by Phytophthora infestans fungus is one of the important diseases in potato plants. The decrease in potato production due to P. infestans can reach 90%. Until now, P. infestans pathogen attack is an important problem and there is no fungicide that is really effective against the disease. This study aims to examine the effectiveness of endophytic fungi from potato roots in suppressing the development of P. infestans potato leaf blight disease carried out at University Farm Stasiun Riset Bener Meriah (UFBM) Syiah Kuala University Tunyang Village, Timang Gajah District, Bener Meriah Regency from May to October 2014. The method used is the experimental method. The results of this study indicate that endophytic fungi from the roots of potato plants in coffee skin compost media can suppress the development of leaf blight caused by P. infestans, endophytic fungi from potato plant roots in coffee skin compost media. The best results were found in B9 endophytic fungi isolates with the intensity of the pathogen attack P. infestans 48.00%, the intensity of damage to potato plants due to pathogen P. infestans and 2.60%, the weight of healthy tubers 332.4 grams.


2018 ◽  
Author(s):  
Milda Ernita ◽  
trimurti habazar ◽  
jamsari ◽  
nasrun

In modern cultivation processes indiscriminate use of pesticides and fertilizers, has led to substantialpollution of soil, air and water. So, there is an urgent need to solve the problem. Rhizobacteria are bacteria thatcolonize plant roots, and these bacteria are known to stimulate growth and thereby reduce incidence of plantdisease by direct and indirect mechanisms. A total of 136 rhizobacteria isolates were isolated from differentrhizosphere soils in central areas of production of onions in Indonesia. These isolates were screened for theircapability to enhance growth and protect onions against bacterial leaf blight disease-caused by Xanthomonasaxonopodis pv.allii. The results showed that ten isolates can enhance growth and protect onions against bacterialleaf blight diseases. Five isolates were isolated from West Sumatra, four isolates from Java and one isolated fromNorth Sumatra. All isolates produced indol-3-acetic acid with different concentrations. Molecular identificationof ten isolates belong to Bacillus sp, Pseudomonas sp, Stenotrophomonas sp and Serratia sp.


2013 ◽  
Vol 13 (2) ◽  
pp. 167-178
Author(s):  
Zurai Resti ◽  
Trimurti Habazar ◽  
Deddi Prima Putra ◽  
Nasrun Nasrun

Screening and identification of endophytic bacteria to control bacterial leaf blight disease on Shallot. The experiment was conducted in Laboratory and Green House, from January to June 2012.   Laboratory experiment consisted of three steps:  (1) isolation of endophytic bacteria from healthy onion roots, (2)In planta /screening of endophytic isolates capable of reducing bacterial leaf blight disease, and (3) molecular identification of potential endophytic isolates.  Treatments of  in planta test were arranged in Completely Randomized Design.  Collected isolates were tested for their capability in controlling bacterial leaf blight disease on shallot.  The variables observed were disease incidence, disease severity, and shallot yield. The results showed that out of 82 isolates successfully isolated, 56 isolates (68.29%) were Gram positive, and 26 isolate (31.71%) were Gram negative. All isolates were HR negative and pathogenicity negative. Six endophytic isolates showed  better performance in inducing resistance and increasing onion yield. Based on 16S rRNA sequence the six isolates were  Bacillus cereus strain P14 , Bacillus cereus strain Se07, Bacillus sp H1, Bacillus sp SJ1 and Serratia marcescens strain PPM4.


2019 ◽  
Vol 1 (2) ◽  
pp. 229-244
Author(s):  
Anggi Anwar Hendra Nurdika ◽  
Suhartiningsih Dwi Nurcahyanti

Soybean leaf blight (Pseudomonas syringae pv glycinea) is one of the important diseases that has the potential to cause production losses of around 11-20%. This study aims to determine the potential of Pseudomonas fluorescens bacteria that is applied through seed encapsulation methods to stimulate soybean plant growth and surpress the development of leaf blight disease. This research was conducted at the Agrotechnopark Jubung greenhouse, University of Jember using a Completely Randomized Design consisting of 5 treatments namely control (P0), encapsulation of P.fluorescens bacteria with compost (P1) formulation, compost + kaolin (P2), compost + talc (P3), and compost + zeolite (P4). The results showed that seed encapsulation with P.fluorescens bacteria made from compost and inorganic carriers was able to reduce the severity of soybean blight disease by up to 40 HSI. Seed encapsulation with P.fluorescens compost + zeolite formulation can increase seed germination, plant height, and number of leaves. The use of talc carrier materials is able to maintain a bacterial population of 6.0 x 103 cfu / ml for up to 28 days of storage. Keywords:  formulation, leaf blight, Pseudomonas fluorescens, Pseudomonas syringae, seed encapsulation


2018 ◽  
Vol 6 (2) ◽  
pp. 134
Author(s):  
Endang Kantikowati ◽  
Ridwan Haris ◽  
Saiful Anwar

The Experiment  was aimed to assess influence of concentration and application of biological agent Paenibacillus polymixa to bacterial leaf blight disease and  black rice yields  (Oryza sativa L.) local varieties The research was conducted in P4S Al Mukhlis   Kiangroke Village, Bandung District, West Java. In December 2016 until April 2017. The experiment used a split-plot design with two factor : 3 application period (W) as the main plot and 4 concentration level (K) as the subplot, thus obtained 12 treatment combinations and each combination was repeated 3 times. Then obtained 36 plot of experiment. Factor I main plot (W) : W1 = morning application (10, 20, 30, 40, 50, 60, 70 days after planting), W2 = daytime (10, 20, 30, 40, 50, 60, 70 days after planting), W3 = afternoon application (10, 20, 30, 40, 50, 60, 70 days after planting) and factor II (K) : K1 = Control, K2 = 5 ml L-1, K3 = 10 ml L-1, K4 = 15 ml L-1. The result showed that the effect of concentration  and time of application of biological agent of Paenibacillus polymixa gave no significant effect on plant height, disease intensity, number of productive tillers, number of the dry grain harvest, number of unhulled grain per panicle, and weight of 1000 filled grains. There was an interaction between concentration and time of application of biological agent of Paenibacillus polymixa to bacterial leaf blight disease intensity at age of 95 DAP, treatment of K3 (10ml L-1) with W1 (morning application)   gave more resistance effect to bacterial leaf blight intensity. 


2015 ◽  
Vol 1 (1) ◽  
pp. 81
Author(s):  
Milda Ernita, Trimurti Habazar, Nasrun Jamsari

In modern cultivation processes indiscriminate use of pesticides and fertilizers, has led to substantialpollution of soil, air and water. So, there is an urgent need to solve the problem. Rhizobacteria are bacteria thatcolonize plant roots, and these bacteria are known to stimulate growth and thereby reduce incidence of plantdisease by direct and indirect mechanisms. A total of 136 rhizobacteria isolates were isolated from differentrhizosphere soils in central areas of production of onions in Indonesia. These isolates were screened for theircapability to enhance growth and protect onions against bacterial leaf blight disease-caused by Xanthomonasaxonopodis pv.allii. The results showed that ten isolates can enhance growth and protect onions against bacterialleaf blight diseases. Five isolates were isolated from West Sumatra, four isolates from Java and one isolated from North Sumatra. All isolates produced indol-3-acetic acid with different concentrations. Molecular identification of ten isolates belong to Bacillus sp, Pseudomonas sp, Stenotrophomonas sp and Serratia sp.


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