scholarly journals Effects of Different Selenium Concentrations on Physiology of Prunus davidiana Seedlings

2019 ◽  
Vol 136 ◽  
pp. 06003
Author(s):  
Liu Yang ◽  
Qinyuan Li ◽  
Junjiang Shu ◽  
Ling Xiao ◽  
Xinyu Gao ◽  
...  

To study the effects of selenium on physiology, Prunus davidiana seedlings were treated with the irrigation of different selenium concentrations solution. The result showed that the photosynthetic pigment content was relatively higher at selenium concentrations of 0, 0.05 and 0.1 mg/L except for carotenoid content which was the highest at 0.1 mg/L but the lowest in control seedlings. In terms of antioxidant enzyme activity, the activities of peroxidase (POD), catalase (CAT) and superoxide dismutase (SOD) in selenium-treated seedlings were all higher than that in control seedlings and especially at the selenium concentration of 1 mg/L. The soluble protein content accumulated most at 0.25 mg/L selenium concentration. The content of malondialdehyde (MDA), as membrane lipid peroxidation index, was the lowest at selenium concentration of 0.5 mg/L. In a word, irrigating lower selenium concentrations could increase the photosynthetic pigment content and higher selenium concentrations enhanced the antioxidant enzyme activity to increase cell protection and kept the P. davidiana seedlings good growth and resistance to adverse environments.

2019 ◽  
Vol 136 ◽  
pp. 06002
Author(s):  
Liu Yang ◽  
Bixia Zheng ◽  
Xiao Zhang ◽  
Tonghao Cui ◽  
Xianmin Xia ◽  
...  

In order to study the changes of the photosynthetic pigment content, antioxidant enzyme activity and osmotic adjuster content, the Prunus davidiana seedlings were cultured into Hoagland nutrient solution which added various concentrations of iron solution. Then the results showed that photosynthetic pigment content and antioxidant enzyme activity were all higher in iron-treated P. davidiana seedlings, compared with that in control seedlings. When increased iron concentration up to 10 mg/L, the chlorophyll a, chlorophyll b and the total chlorophyll content gradually rose, but decreased at iron concentrations of 20, 30, 40 and 60 mg/L. Irrigating iron concentration of 60 mg/L was the best way to increase the activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and malondialdehyde (MDA) content. But for chlorophyll a/b ratio, it was the highest in no-iron seedlings. Moreover, the soluble protein content was the highest at iron concentration of 30 mg/L, but the lowest at 10 mg/L. Thus, the study concluded that irrigating iron solution could improve the growth and resistance to adverse circumstances of P. davidiana seedlings.


2020 ◽  
Vol 218 ◽  
pp. 03007
Author(s):  
Renyan Liao ◽  
Qian Chen

A pot experiment was conducted to study the effects of cross-hybridization on biomass, photosynthetic pigment content, antioxidant enzyme activity and soluble protein content of Solanum nigrum and Solanum diphyllum. The results showed that under cadmium (Cd) stress, the plant biomass, photosynthetic pigment content, antioxidant enzyme activity and soluble protein content of hybrid ND (S. nigrum male × S. diphyllum female) and DN (S. diphyllum male × S. nigrum female) were all higher than those of S. nigrum. S. diphyllum was optimal, and hybrid ND was superior to hybrid DN. The POD activity of S. nigrum was higher than that of S. diphyllum, and the POD activity of hybrid ND and DN was significantly higher than that of parent.


2021 ◽  
Author(s):  
A. I. Muhammad ◽  
A. M. Dalia ◽  
T. C. Loh ◽  
H. Akit ◽  
A. A. Samsudin

Abstract This study compares the effects of sodium selenite, selenium yeast, and enriched bacterial organic selenium protein on antioxidant enzyme activity, serum biochemical profiles, and egg yolk, serum, and tissue selenium concentration in laying hens. In a 112-d experiment, 144 Lohman Brown Classic hens, 23-wks old were divided into four equal groups, each has six replicates. They were assigned to 4 treatments: 1) a basal diet (Con), 2) Con plus 0.3 mg/kg feed sodium selenite (SS); 3) Con plus 0.3 mg/kg feed Se-yeast (SY): 4) Con plus 0.3 mg/kg feed bacterial enriched organic Se protein (ADS18) from Stenotrophomonas maltophilia bacteria. On d 116, hens were euthanized (slaughtered) to obtain blood (serum), liver organ, and breast tissue to measure antioxidant enzyme activity, biochemical profiles, and selenium concentration. The results show that antioxidant enzyme activity of hens was increased when fed bacterial organic Se (ADS18), resulting in a significant (P < 0.05) increase in serum GSH-Px, SOD, and CAT activity compared to other treatment groups. However, ADS18 and SY supplementation increase (P < 0.05) hepatic TAC, GSH-Px, and CAT activity, unlike the SS and Con group. Similarly, dietary Se treatment reduced total cholesterol and serum triglycerides concentrations significantly (P < 0.05) compared to the Con group. At 16 and 18 weeks, selenium concentration in hen egg yolks supplemented with dietary Se was higher (P < 0.05) than in Con, with similar patterns in breast tissue and serum. Supplementation with bacterial organic Se (ADS18) improved antioxidant enzyme activity, decreased total serum cholesterol and serum lipids, and increased Se deposition in egg yolk, tissue, and serum. Hence, organic Se may be considered a viable source of Se in laying hens.


2003 ◽  
Vol 14 (03) ◽  
pp. 134-143 ◽  
Author(s):  
James J. Klemens ◽  
Robert P. Meech ◽  
Larry F. Hughes ◽  
Satu Somani ◽  
Kathleen C.M. Campbell

This study's purpose was to determine if a correlation exists between cochlear antioxidant activity changes and auditory function after induction of aminoglycoside (AG) ototoxicity. Two groups of five 250-350 g albino guinea pigs served as subjects. For 28 days, albino guinea pigs were administered either 200 mg/kg/day amikacin, or saline subcutaneously. Auditory brainstem response testing was performed prior to the first injection and again before sacrifice, 28 days later. Cochleae were harvested and superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase, glutathione reductase activities and malondialdehyde levels were measured. All antioxidant enzymes had significantly lower activity in the amikacin group (p ≤ 0.05) than in the control group. The difference in cochlear antioxidant enzyme activity between groups inversely correlated significantly with the change in ABR thresholds. The greatest correlation was for the high frequencies, which are most affected by aminoglycosides. This study demonstrates that antioxidant enzyme activity and amikacin-induced hearing loss significantly covary.


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