scholarly journals Profiling Analysis of Fatty Acids and Collagens Obtained from Sea Cucumbers

2020 ◽  
Vol 141 ◽  
pp. 03006
Author(s):  
Pascal Budzinski ◽  
Mananya Maimeun ◽  
Parita Mutrakulcharoen ◽  
Benjamaporn Wonganu ◽  
Malinee Sriariyanun
Keyword(s):  
Fatty Acids ◽  
Sea Cucumber ◽  
Functional Foods ◽  
Unsaturated Fatty Acids ◽  
Biological Activities ◽  
Extraction Methods ◽  
Chemical Extraction ◽  
Sea Cucumbers ◽  
Ft Ir ◽  
Profiling Analysis

Investigations of alternative resources for production of functional foods and ingredients containing valuable compounds with biological activities are getting more and more attention. Sea cucumbers are aquatic functional foods with various medical and pharmaceutical effects, such as antioxidant, antibacterial, antifungal, antiviral, anti-inflammatory and neuroprotective activities. This study aimed to conduct profiling analysis of fatty acids and collagens extracted from four different sea cucumbers harvested from Papua New Guinea by using Gas Chromatrography-Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectrometer (FT-IR). Three different extraction methods in combination with various solvents were used to find the best combination for extracting fatty acids. Enzymatic and chemical extraction methods were applied for collagen extraction. The highest fat recovery in this study was 85.32% of theoretical yield with high proportions of unsaturated fatty acids up to 74.54%, and enriched with omega-3 fatty acid. FT-IR chromatogram of sea cucumber protein extracts showed the characteristic of collagen enriched with glycine and proline. The nutritional analysis of sea cucumber extracts demonstrated the potential use as functional foods and ingredients with high benefits to human health.

scholarly journals An Alternative Approach for the Synthesis of Sulfoquinovosyldiacylglycerol

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4275
Author(s):  
Tobias Sitz ◽  
Hendrik Domey ◽  
Judith Fischer ◽  
Sascha Rohn
Keyword(s):  
Fatty Acids ◽  
Functional Foods ◽  
Unsaturated Fatty Acids ◽  
Biological Activities ◽  
Saturated Fatty Acids ◽  
Diverse Range ◽  
Natural Sources ◽  
Model Studies ◽  
Alternative Approach ◽  
Increasing Demand

Sulfoquinovosyldiacylglycerol (SQDG) is a glycolipid ubiquitously found in photosynthetically active organisms. It has attracted much attention in recent years due to its biological activities. Similarly, the increasing demand for vegan and functional foods has led to a growing interest in micronutrients such as sulfolipids and their physiological influence on human health. To study this influence, reference materials are needed for developing new analytical methods and providing enough material for model studies on the biological activity. However, the availability of these materials is limited by the difficulty to isolate and purify sulfolipids from natural sources and the unavailability of chemical standards on the market. Consequently, an alternative synthetic route for the comprehensive preparation of sulfolipids was established. Here, the synthesis of a sulfolipid with two identical saturated fatty acids is described exemplarily. The method opens possibilities for the preparation of a diverse range of interesting derivatives with different saturated and unsaturated fatty acids.

scholarly journals TERIPANG SEBAGAI SALAH SATU SUMBER KOLAGEN

OSEANA ◽  
2020 ◽  
Vol 45 (1) ◽  
pp. 17-27
Author(s):  
Bayu Kumayanjati
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Protein Content ◽  
Sea Cucumber ◽  
Unsaturated Fatty Acids ◽  
High Protein ◽  
Sea Cucumbers ◽  
High Protein Content ◽  
Alternative Sources

Sea cucumbers are one of the Echinoderms that are rich in nutrients. Sea cucumbers contain a lot of minerals, unsaturated fatty acids, amino acids, and quite high protein. Protein content in sea cucumbers can reach 40% depending on the type and habitat of the sea cucumber. High protein content in sea cucumbers has the potential as a source of collagen. Collagen is a constituent protein found in the skin, tendons, cartilage and hard bones, and other tissues. Collagen currently has been widely applied in various industrial fields, such as cosmetics, health, pharmacy and food, so that the requirement for collagen will increase. Collagen currently is still dominated by collagen from cattle and pigs. Collagen from cattle is the most widely used in Indonesia because the halal collagen is important in Indonesia. The requirement for halal collagen will certainly be limited if it only relies on collagen from cattle. Therefore, alternative sources of collagen are needed, one of them is sea cucumbers.

scholarly journals Characterization and Biological Activities of Seed Oil Extracted from Berberis dasystachya Maxim. by the Supercritical Carbon Dioxide Extraction Method

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1836
Author(s):  
Lijuan Han ◽  
Qingqing Han ◽  
Yongjing Yang ◽  
Honglun Wang ◽  
ShuLin Wang ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Supercritical Carbon Dioxide ◽  
Extraction Method ◽  
Unsaturated Fatty Acids ◽  
Biological Activities ◽  
Maximum Yield ◽  
Dependent Manner ◽  
Supercritical Carbon

Characterization of the structure and pharmacological activity of Berberis dasystachya Maxim., a traditional Tibetan medicinal and edible fruit, has not yet been reported. In this study, central composite design (CCD) combined with response surface methodology (RSM) was applied to optimize the extraction conditions of B. dasystachya oil (BDSO) using the supercritical carbon dioxide (SC-CO2) extraction method, and the results were compared with those obtained by the petroleum ether extraction (PEE) method. The chemical characteristics of BDSO were analyzed, and its antioxidant activity and in vitro cellular viability were studied by DPPH, ABTS, reducing power assay, and MTT assay. The results showed that the maximum yield of 12.54 ± 0.56 g/100 g was obtained at the optimal extraction conditions, which were: pressure, 25.00 MPa; temperature 59.03 °C; and CO2 flow rate, 2.25 SL/min. The Gas chromatography (GC) analysis results showed that BDSO extracted by the SC-CO2 method had higher contents of unsaturated fatty acids (85.62%) and polyunsaturated fatty acids (57.90%) than that extracted by the PEE method. The gas chromatography used in conjunction with ion mobility spectrometry (GC–IMS) results showed that the main volatile compounds in BDSO were aldehydes and esters. BDSO also exhibited antioxidant ability in a dose-dependent manner. Moreover, normal and cancer cells incubated with BDSO had survival rates of more than 85%, which indicates that BDSO is not cytotoxic. Based on these results, the BDSO extracted by the SC-CO2 method could potentially be used in other applications, e.g., those that involve using berries of B. dasystachya.

scholarly journals CULTIVATION OF COTTON IN CHINA AND IRAN WITH CONSIDERING BIOLOGICAL ACTIVITIES AND ITS HEALTH BENEFITS

2020 ◽  
Vol 53 (1) ◽  
pp. 105-120 ◽  
Author(s):  
W. SUN ◽  
M. H. SHAHRAJABIAN ◽  
M. KHOSHKHARAM ◽  
H. SHEN ◽  
Q. CHENG
Keyword(s):  
Fatty Acids ◽  
Seed Oil ◽  
Unsaturated Fatty Acids ◽  
Animal Feed ◽  
Biological Activities ◽  
Saturated Fatty Acids ◽  
Health Benefits ◽  
Cattle Feed ◽  
Important Health ◽  
Pharmaceutical Industries

Cotton (Gossypium L.) is one of the most important commercial crops and it is famous as white gold. Cotton has a diversity of applications, principally medicinal and many other usages, such as pigments, derivatives for cattle feed, different uses of the oil extracts and etc. Cottonseed oil has a ration of 2:1 of polyunsaturated to saturated fatty acids and generally consists of 65-70% unsaturated fatty acids, including 18-24% monounsaturated (oleic) and 42-52% polyunsaturated (linoleic), and 26-35% saturated (palmitic and stearic). The most important health benefits of cotton is treat respiratory diseases, treat skin problems, treat wounds, beneficial for breastfeeding mothers, a good cure for rat bite, an appropriate cure for scorpion bite, for joint and eye pains, for swollen legs, for removing bacteria in teeth, and alternative medicine for various diseases such as cancer, HIV and etc. Cotton seed oil mostly extracted from Gossypium hirsutum and Gossypium herbaceum, that are also grown for cotton fiber and animal feed. Gossypol is one of the most effective ingredients, both in traditional pharmaceutical practices and alternative modern medicinal preparations. It is a toxic polyphenolic bisesquiterpene, which may have antifertility and antiviral properties. The obtained findings suggest potential of cotton as a natural resource in pharmaceutical industries.

scholarly journals Identification of circRNA-Associated-ceRNA Networks Involved in Milk Fat Metabolism under Heat Stress

2020 ◽  
Vol 21 (11) ◽  
pp. 4162
Author(s):  
Dongyang Wang ◽  
Zujing Chen ◽  
Xiaona Zhuang ◽  
Junyi Luo ◽  
Ting Chen ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Heat Stress ◽  
Mammary Gland ◽  
Dairy Cows ◽  
Milk Fat ◽  
Unsaturated Fatty Acids ◽  
Negative Impact ◽  
Biological Activities ◽  
Fat Metabolism ◽  
Milk Quality

Summer temperatures are generally high in Southern China, and cows are likely to suffer a heat stress reaction. Heat stress will have a negative impact on the performance of dairy cows; however, the mechanism by which high temperature affects lactation is not clear. CircRNA is a type of non-coding RNA discovered in recent years, which performs a crucial function in many biological activities. However, the effects of circRNA on lactation function of dairy cows under heat stress is unknown. The present study aimed to explore the expression levels of circRNA in the mammary gland tissue of cows under heat stress. Firstly, we collected blood and milk samples of summer and winter cows and evaluated lactation performance using serum indicators, milk production, and milk composition. Incorporating the calculation of the temperature and humidity index, we conformed the heat stress status of cows in summer. Heat stress increased the concentration of HSP70 and decreased the concentration of SOD and PRL. Heat stress not only reduced milk yield but also affected milk quality, with milk lactose and milk protein decreasing with increased temperature. The analysis of the fatty acid composition in summer milk found significantly reduced concentrations of unsaturated fatty acids, especially long-chain unsaturated fatty acids. Sequencing of the cow’s mammary gland transcriptome revealed that compared to the appropriate temperature (ST) group, the heat stress (HS) group had a total of 2204 upregulated and 3501 downregulated transcripts. GO enrichment and KEGG pathway analysis showed that these genes were mainly related to milk fat metabolism. In addition, 19 upregulated and 19 downregulated circRNA candidates were found in response to heat stress. We used Pearson’s test to establish the correlation of circRNA-mRNA and identified four pairs of circRNA-miRNA networks between four circRNAs, six miRNAs, and the CD36 gene. In this study, we revealed the possible role of circRNAs in lactation of dairy cows and identified that circRNA-miRNA-mRNA networks might exist in the cow’s mammary glands, providing valuable experience for dairy lactation and milk quality.

An overview of dietary supplements on obesity and type 2 diabetes: efficacy and mechanisms

2021 ◽  
Vol 22 ◽  
Author(s):  
Xiaohong Liu ◽  
Xin Zeng ◽  
Wen Liu ◽  
Yanrong Lu ◽  
Jingqiu Cheng ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Fatty Acids ◽  
Dietary Supplements ◽  
Unsaturated Fatty Acids ◽  
Biological Activities ◽  
Dietary Factors ◽  
Dietary Therapy ◽  
Β Cell ◽  
Bioactive Ingredients

: Obesity is a common nutritional disorder, associated with a variety of chronic diseases, among them, type 2 diabetes (T2DM) has emerged as a serious worldwide health problem. Insulin resistance and β cell dysfunction are the main pathological characteristics of T2DM, and obesity and hyperlipidemia are the critical causal factors. It is commonly accepted that dietary factors are of paramount importance in the management of obesity and T2DM. Particularly, many botanic products and their extracts are endowed with a wide spectrum of biological activities, making them extensively studied as anti-obesity and anti-diabetes dietary supplements or new drug candidates. In this review, we aimed to summarize the effects, related mechanisms, and safety issues of dietary continents on obesity and T2DM, to provide theoretical support for better research and development of dietary therapy strategy for the treatment of obesity and T2DM. Based on a bunch of clinical investigations, specific carbohydrates and fatty acids, such as dietary fibers, polysaccharides, unsaturated fatty acids, have hypoglycemic and hypolipidemic effects. Vitamin D plays important role in metabolism and immunity modulation. Apart from them, natural bioactive ingredients from plants, such as flavonoids, polyphenols, alkaloids, terpenoids, polysaccharides, and quinones are efficient in helping weight loss and improving insulin sensitivity and glycemic control. They can protect β cell function by anti-inflammation, anti-oxidation, and anti-apoptosis properties, as well as regulating lipid metabolism. Therefore, promoting the consumption of diverse natural bioactive ingredients-rich products could be an effective nutritional strategy to benefit patients with obesity and type 2 diabetes.

Optimum Level of Dietary n-3 Highly Unsaturated Fatty Acids for Juvenile Sea Cucumber,Apostichopus japonicus

2015 ◽  
Vol 46 (6) ◽  
pp. 642-649 ◽  
Author(s):  
Mingling Liao ◽  
Tongjun Ren ◽  
Wei Chen ◽  
Zhiqiang Jiang ◽  
Huan Yang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Sea Cucumber ◽  
Unsaturated Fatty Acids ◽  
Apostichopus Japonicus ◽  
Optimum Level ◽  
Highly Unsaturated Fatty Acids

scholarly journals Composition of Fatty Acids in Evaluation of Sea Cucumber Potency for Nutraceutical Product Development

2017 ◽  
Vol 11 (2) ◽  
pp. 69
Author(s):  
Rini Susilowati ◽  
Asri Pratitis ◽  
Hedi Indra Januar
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Product Development ◽  
Sea Cucumber ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Resonance Method ◽  
Raw Material ◽  
Nuclear Magnetic Resonance Method ◽  
Fatty Acids Composition

Unsaturated fatty acid in food has an effect to improve human health, while saturated fatty acid has the opposite impact. Ratio between both types of fatty acids is important to evaluate the biological material for nutraceutical product development. This paper aimed to evaluate the nutraceutical potency of sea cucumber from Kendari Bay Waters, South East Sulawesi, based on their fatty acids composition. The analysis was conducted by the 1H-NMR (Hidrogen-Nuclear Magnetic Resonance) method. Several species within 4 genera (Holothuria, Bohadschia, Actinopyga, and Stichopus) were selected as the subject of the study. Comparison of fatty acid composition has been done in genera level, the preliminary study found that variation between species among the same genera was insignificant (p >0.05). Results of the study detected that Holothuria contained with an even ratio of Poly Unsaturated Fatty Acids (PUFA) and Saturated Fatty Acids (SFA), but Actinopyga was detected with low PUFA/SFA ratio. It may suggest that Holothuria from this region is the most potential raw material for nutraceutical product development. Meanwhile, precaution is needed for genera that contained with low PUFA/SFA ratio. Furthermore, comparison with other studies detected that fatty acids composition is not morphological specific, but vary, according to food viability, environmental and geographic location. Therefore, preliminary screening is important in evaluation of sea cucumber material for nutraceutical product development.  

scholarly journals Chemical prospection of moringa oil and bromatological quality of the pie from different types of grain processing

2020 ◽  
Vol 9 (11) ◽  
pp. e82291110599
Author(s):  
Bárbara Lemes Outeiro Araújo ◽  
Ednilton Tavares de Andrade ◽  
Jaqueline Damiany Portela ◽  
Rafael Peron Castro ◽  
Pedro Castro Neto
Keyword(s):  
Fatty Acids ◽  
Crude Protein ◽  
Extraction Methods ◽  
Chemical Extraction ◽  
Grain Processing ◽  
Air Temperatures ◽  
Different Temperatures ◽  
Moringa Oil ◽  
Mechanical Extraction

Moringa oleifera Lam. is a drought-resistant plant and able to survive in poor soils, obtaining up to three harvests per year. The objective of this work was to study the chemical prospecting of the oil and the bromatological quality of the moringa cake amog different temperatures of drying (40, 55, and 70 ºC) as well as from the oil chemical and mechanical extraction methods. The extracted oils were qualitatively evaluated for acidity, peroxide and iodine levels, as well as the chemical composition of fatty acids by gas chromatography, of samples dried at different drying air temperatures. The pies from mechanical extractions were evaluated for water content, ether extract, crude protein, ash and fibers in neutral detergent. The drying air temperatures of 40, 55, and 70 ºC significantly affected the physical-chemical quality of the oil and the moringa cake, with the best result being the samples from the dry grains at 40 °C. The composition of the main fatty acids was not altered according to the statistical method applied, these being oleic fatty acid (73.60 to 77.07%), erucic (5.65 to 6.67%) and palmitoleic (4.90 to 5.72%). The chemical extraction of oil, although more efficient than the mechanical one, presented higher levels of acidity and peroxide. The content of fibers in neutral detergent and crude protein of the pie decreased significantly for dried grains with drying air temperature above 40 °C.

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