scholarly journals Sonneratia alba Extract Protects the Post Larvae of Tiger Shrimp Penaeus monodon against Vibrio harveyi and Saprolegnia sp.

2020 ◽  
Vol 147 ◽  
pp. 01004
Author(s):  
Gina Saptiani ◽  
Andi Noor Asikin ◽  
Fikri Ardhani
Keyword(s):  
Survival Rate ◽  
Vibrio Harveyi ◽  
Saline Water ◽  
Penaeus Monodon ◽  
Clinical Signs ◽  
Effective Dosage ◽  
Relative Percentage ◽  
Tiger Shrimp ◽  
Percentage Survival ◽  
Sonneratia Alba

The research aimed to study Sonneratia alba leaves extract to inhibit Vibrio harveyi and Saprolegnia sp., and to evaluate its effectiveness to protect the post larvae of tiger shrimp against the infections. The leaves of S. alba were cleaned, chopped, dried, macerated and extracted in three different solvents namely ethanol 80%, freshwater and saline water. The post larvae 8 then immersed with the extract and infected with Vibrio harveyi and Saprolegnia sp. The clinical signs, pathological anatomy, total vibrio count, prevalence, survival rate, and relative percentage of survival were observed. The results showed that S. alba leaves extract inhibited V. harveyi and Saprolegnia sp. on post larvae of tiger shrimp, improve survival rate and relative percentage survival. The most effective dosage was 1,250 mg/L either for ethanolic, freshwater and saline water solvents.

scholarly journals Response of hemocytes profile in the black tiger shrimp (Penaeus monodon) against Vibrio harveyi induced by Xylocarpus granatum leaves extract

2020 ◽  
Vol 13 (4) ◽  
pp. 751-757 ◽  
Author(s):  
Gina Saptiani ◽  
A. Syafei Sidik ◽  
Fikri Ardhani ◽  
Esti Handayani Hardi
Keyword(s):  
Survival Rate ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Water Extract ◽  
Negative Control ◽  
Distilled Water ◽  
Black Tiger Shrimp ◽  
Xylocarpus Granatum ◽  
Tiger Shrimp ◽  
Immersing Method

Aim: The present study investigated hemocytes profile of black tiger shrimp (Penaeus monodon) induced with Xylocarpus granatum leaves extract to protect against Vibrio harveyi infection. Materials and Methods: X. granatum leaves were chopped into small size, air-dried, and extracted with one of the following solvents: Ethanol, distilled water, and seawater, whereas each solvent was given in three different concentrations (750 ppm, 1.000 ppm, and 1.250 ppm, respectively). Extracts were induced to 60 post-larvae shrimp in each treatment (three replicates, 20 shrimp for each) by immersing method and subsequently challenged with V. harveyi. Results: This study demonstrated different effectiveness among solvents used to extract X. granatum leaves, in which distilled water showed the most effective solvent as can be seen from the lowest percentage on anorexia, lethargic, and weakened reflex of shrimp compared with another solvent, positive and negative controls. Pathological symptoms for shrimp induced by X. granatum leaves extract were minimum with the highest survival rate compared with those of positive and negative control. Total hemocyte cells and its cell constituents such as semi-granular, granular, and hyaline cells on treatment group at 1.250 ppm were higher than controls. Conclusion: Leaves extract of X. granatum extract effectively inhibited V. harveyi infection, increased survival rate, and hemocytes cell of the experimental shrimp. Distilled water extract of X. granatum at 1.250 ppm demonstrated the highest protective effect toward V. harveyi infection on P. monodon.

scholarly journals Screening of probiotics bacteria from coral reef using co-culture method for controlling vibriosis in tiger shrimp (Penaeus monodon) larvae

2015 ◽  
Vol 10 (2) ◽  
pp. 174
Author(s):  
Ade Dwi Sasanti ◽  
Widanarni . ◽  
Sukenda .
Keyword(s):  
Coral Reef ◽  
Survival Rate ◽  
16S Rrna ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Culture Method ◽  
In Vivo Test ◽  
Tiger Shrimp

<p>ABSTRACT</p><p><br />This study was carried out to obtain bacteria isolates from coral reef using co-culture method which potentially inhibit Vibrio harveyi growth. A total of 110 isolates were isolated from Acropora sp., Merulina sp., Hystrix sp., Poecillophora sp., Porites sp., and Haliophora sp., and were screened for their antagonistic activity against V. harveyi in in vitro and in vivo test. Five candidate probiotics (5H1 candidate probiotics isolated from Acropora sp., 11I and 11G isolates isolated from Hystrix sp. and 13B and 13G1 isolates isolated from Poecillophora sp., was able to inhibit growth of V. harveyi MR5339 RFR up to 101‒102 cfu/mL. Two isolates (13B and 13G1) were not pathogenic at concentration 106 cfu/mL bacteria and could increase of survival rate of tiger shrimp (Penaeus monodon) larvae in in vivo test. Survival rate of tiger shrimp larvae that treatment with 13B and 13G1 were 86,67% and 88,33%, and have a significant different with positive control (61,67%). Partial sequencing of 16S-rRNA showed that 13G1 isolate was similar to V. alginolyticus.<br />Keywords: vibriosis, Vibrio harveyi, tiger shrimp, probiotic, coral reef</p><p><br />ABSTRAK</p><p><br />Penelitian ini bertujuan untuk mendapatkan bakteri probiotik asal terumbu karang dengan metode kultur bersama untuk pengendalian penyakit vibriosis pada larva udang windu (Penaeus monodon). Sebanyak 110 isolat berhasil diisolasi dari Acropora sp., Merulina sp., Hystrix sp., Poecillophora sp., Porites sp., dan Heliophora sp. dan dilakukan penapisan untuk melihat aktivitas kemampuannya melawan Vibrio harveyi MR 5339 RfR dalam uji in vitro dan in vivo. Sebanyak 56 isolat menghasilkan daya hambat terhadap V. harveyi MR5339 RfR pada metode kultur bersama. Lima isolat kandidat probiotik (isolate 5H1 diisolasi dari Acropora sp., isolat 11I dan 11G diisolasi dari Hystrix sp., serta isolat 13B dan 13G1 yang diisolasi dari Poecillophora sp.), mampu menekan pertumbuhan V. harveyi MR5339 RfR hingga 101–102 cfu/mL. Kedua isolat (13B dan 13G1) terbukti tidak bersifat patogen pada konsentrasi 106 cfu/mL dan mampu meningkatkan sintasan larva udang windu pada uji in vivo. Nilai sintasan larva pada perlakuan yang diberi kandidat probiotik 13B dan 13G1 berturut-turut adalah 86,67% dan 88,33%, namun berbeda nyata dengan kontrol positif (61,67%). Hasil analisis sekuen sebagian gen 16S-rRNA menunjukkan bahwa isolat 13G1 termasuk spesies V. algynoliticus.<br /><br />Kata kunci: vibriosis, Vibrio harveyi, udang windu, probiotik, terumbu karang</p>

scholarly journals Dietary mannan oligosaccharide enhances growth and survival of juvenile tiger shrimp Penaeus monodon against experimental challenge with Vibrio harveyi and white spot syndrome virus

2020 ◽  
Vol 67 (4) ◽  
Author(s):  
N. Felix ◽  
P. Kalaimani Arasi ◽  
K. Manikandan
Keyword(s):  
Weight Gain ◽  
Survival Rate ◽  
White Spot Syndrome Virus ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
White Spot ◽  
Post Challenge ◽  
Feeding Trial ◽  
Growth And Survival ◽  
Tiger Shrimp

A 45-days feeding trial was conducted on juvenile tiger shrimps Penaeus monodon of size 0.997±0.098 g with diets containing Mannan oligosaccharides (Mos) at three graded levels (0.1, 0.2 and 0.3%). Maximum weight gain (1.353 g) was achieved in shrimps fed with 0.3% Mos concentration, followed by 0.2% Mos (1.224 g). Best feed conversion ratio (FCR) of 2.406 was registered in 0.3% Mos groups. Results indicate that there was a significant difference (p<0.01) in weight gain and FCR among the treatments. However, no statistical differences were observed on the specific growth rate (SGR), mean feed intake and survival rate. Feeding trial was followed by a 10 days challenge study with Vibrio harveyi by intramuscular injection and white spot syndrome virus (WSSV) by feeding WSSV infected tissues. Significantly higher survival rate was observed in the shrimp groups fed with 0.3% Mos (66.67%) than the other treatments when challenged with V. harveyi. Tiger shrimps fed with 0.3% Mos challenged with WSSV showed a significantly higher survival rate (66.67%), followed by 0.2% Mos (50%), 0.1% Mos (25%) and control (25%) on day 5 post-challenge. However On day 10 post-challenge with WSSV, except in the groups fed with 0.3% Mos (16.67% survival), 100% mortality was noticed in all other diet groups. Therefore, it is concluded that dietary administration of Mos at 0.3% would enhance the growth and survival of tiger shrimp against V. harveyi. With respect to WSSV challenge, it appears that, though Mos at 0.3% had a positive effect on the shrimp, higher survival was not observed. Whether additional increment in Mos supplementation in the diet would positively affect the survival of tiger shrimps post-WSSV challenge, needs to be investigated further.

scholarly journals Effects of different doses of skt-b vibrio probiotic bacteria addition on survival and growth rate of tiger shrimp (Penaeus monodon) larva

2010 ◽  
Vol 9 (1) ◽  
pp. 21 ◽  
Author(s):  
. Widanarni ◽  
M.A. Lidaenni ◽  
Dinamella Wahjuningrum
Keyword(s):  
Growth Rate ◽  
Survival Rate ◽  
Pathogenic Bacteria ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Probiotic Bacteria ◽  
Optimum Dose ◽  
Tiger Shrimp ◽  
Survival And Growth ◽  
Different Doses

<p>Probiotic bacteria has been widely used as biocontrol agents in tiger shrimp hatcheries.  <em>Vibrio </em>SKT-b is one of the probiotic bacteria candidates that could suppressed the growth of pathogenic bacteria <em>Vibrio harveyi </em>and could increase survival rate of tiger shrimp larva. This experiment was carried out to study the effects of probiotic bacteria SKT-b <em>Vibrio</em> addition at different doses on survival and growth rate of tiger shrimp larva.  Experiment was conducted with five treatments and three replications, consisted of SKT-b <em>Vibrio</em> probiotic bacteria addition at the doses of 10<sup>3</sup> CFU/ml, 10<sup>4 </sup>CFU/ml, 10<sup>5</sup> CFU/ml, and 10<sup>6</sup> CFU/ml and control (0 CFU/ml).  Results showed that optimum dose of probiotic bacteria for tiger shrimp was 10<sup>4 </sup>CFU/ml with a survival rate of 94.67%. However, the addition of probiotic bacteria at this particular dose did not significantly increase shrimp growth rate as compared with control.</p> <p>Key words: Probiotic bacteria, SKT-b <em>Vibrio</em>, doses, tiger shrimp larva</p> <p> </p> <p>ABSTRAK<br /> Bakteri probiotik telah banyak digunakan sebagai agen biokontrol dalam pembenihan udang windu.  <em>Vibrio </em>SKT-b merupakan salah satu jenis bakteri kandidat probiotik yang telah diuji dapat menekan pertumbuhan bakteri patogen <em>Vibrio harveyi </em>dan dapat meningkatkan kelangsungan hidup larva udang windu.  Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian bakteri probiotik <em>Vibrio</em> SKT-b dengan dosis yang berbeda terhadap kelangsungan hidup dan pertumbuhan larva udang windu. Penelitian ini dilakukan dalam 5 perlakuan dengan masing-masing 3 ulangan, yaitu penambahan bakteri probiotik <em>Vibrio</em> SKT-b dengan dosis 10<sup>3</sup> CFU/ml, 10<sup>4 </sup>CFU/ml, 10<sup>5</sup> CFU/ml, dan 10<sup>6</sup> CFU/ml dan kontrol (0 CFU/ml).  Hasil penelitian menunjukkan bahwa dosis optimal untuk larva udang windu adalah 10<sup>4 </sup>CFU/ml dengan nilai kelangsungan hidup 94,67%. Namun, pemberian bakteri probiotik tersebut belum menghasilkan pertumbuhan yang berbeda nyata dengan kontrol.</p> <p>Kata kunci: Bakteri probiotik, <em>Vibrio</em> SKT-b, dosis, larva udang windu</p>

scholarly journals Inhibitory Mechanism of Robiotic Bacteria on The Growth of Vibrio harveyi in Tiger Shrimp (Penaeus monodon) Larvae

2008 ◽  
Vol 7 (2) ◽  
pp. 179
Author(s):  
. Widanarni ◽  
E. Ayuzar ◽  
. Sukenda
Keyword(s):  
Survival Rate ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Vibrio Alginolyticus ◽  
Probiotic Bacteria ◽  
Rrna Gene ◽  
Inhibitory Mechanism ◽  
Water Culture ◽  
Tiger Shrimp

<p>Three probiotics named SKT-b, 1Ub, and Ua had inhibitory activity against the growth of <em>Vibrio harveyi</em>. These strains were mutated by rifampicin resistant. The inhibitory effect of SKT-b,1Ub, and Ua on the growth of <em>V. harveyi</em> was investigated by concomitant incubation of the two bacteria in a culture shrimp larvae. Colony forming unit of <em>V. harveyi</em>, probiotic and total of bacteria in dead, live larvae and water culture was monitored, and survival rate of larvae was investigated. Shrimp inoculated probiotic previously had survival rate higher than control (without probiotic). Number of <em>V. harveyi </em>in treatment without probiotic inoculation also higher compared to treatment with probiotic inoculation in dead, live larvae and water culture.  It demonstrated possible inhibition of probiotic bacteria on <em>V. harveyi</em> through competition for adherence sites or nutrition source. Partial sequencing of 16S-rRNA gene showed that 1Ub was similar to <em>Pseudoalteromonas piscicida</em>, whereas SKT-b and Ua were similar to <em>Vibrio alginolyticus</em>.</p> <p>Keywords: probiotic bacteria, inhibitory mechanism, <em>V. harveyi</em>, tiger shrimp</p> <p> </p> <p>ABSTRAK</p> <p>Tiga isolat bakteri probiotik yaitu 1Ub, SKT-b dan Ua telah diuji memiliki aktivitas penghambatan terhadap pertumbuhan<em> V. harveyi </em>secara <em>in vitro</em>. Ketiga isolat ini kemudian diberi penanda resisten rifampisin (Rf<sup>R</sup>) melalui mutasi spontan untuk mempelajari mekanisme penghambatannya pada larva udang windu.  Efek penghambatan dari 1Ub, SKT-b dan Ua terhadap pertumbuhan <em>V. harveyi</em> diamati melalui pemberian secara bersamaan antara bakteri probiotik dan <em>V. harveyi</em> tersebut dalam air pemeliharaan larva udang.  Jumlah sel bakteri probiotik,<em> V. harveyi</em> dan total bakteri baik pada larva mati, larva hidup dan air pemeliharaan diamati dan kelangsungan hidup larva dihitung.  Nilai kelangsungan hidup udang pada perlakuan yang diinokulasi bakteri probiotik lebih tinggi daripada kontrol (tanpa penambahan bakteri probiotik). Jumlah sel <em>V. harveyi</em> pada perlakuan tanpa penambahan probiotik juga lebih tinggi, dibanding pada perlakuan dengan penambahan probiotik baik pada larva mati, larva hidup maupun air media pemeliharaan. Hal ini menunjukkan adanya penghambatan bakteri probiotik terhadap <em>V. harveyi</em> yang kemungkinan melalui kompetisi tempat pelekatan atau sumber nutrisi. Hasil analisis sekuen sebagian gen 16-rRNA menunjukkan bahwa isolat 1Ub termasuk spesies <em>Pseudoalteromonas piscicida, </em>sedangkan SKT-b dan Ua termasuk spesies <em>Vibrio alginolyticus</em>. </p> <p>Kata kunci: bakteri probiotik, mekanisme penghambatan, <em>V. harveyi</em>, udang windu</p>

scholarly journals ANALYSIS OF IMMUNE RESPONSES ON TRANSGENIC TIGER SHRIMP (Penaeus monodon) AGAINST PATHOGENIC BACTERIUM Vibrio harveyi

2014 ◽  
Vol 9 (1) ◽  
pp. 23
Author(s):  
Andi Parenrengi ◽  
Bunga Rante Tampangallo ◽  
Andi Tenriulo
Keyword(s):  
Immune Response ◽  
Immune Responses ◽  
Vibrio Harveyi ◽  
Gene Transfection ◽  
Penaeus Monodon ◽  
Pathogenic Bacterium ◽  
Shrimp Culture ◽  
Tiger Shrimp ◽  
Randomized Design ◽  
Study Results

Vibriosis is one of main diseases of the black tiger shrimp Penaeus monodon infected by pathogenic bioluminous bacterium Vibrio harveyi that can cause mass mortalities in shrimp culture. The bacteria can also trigger the disease white spot syndrome virus (WSSV). An effort to produce shrimp disease-resistant strains has been done through transgenesis technology with antiviral gene transfection. By this technology, it is expected an increase in the immune response of shrimp in a variety of diseasecausing pathogens. This study aimed to determine the immune responses (total haemocytes, haemocyte differentiation, and phenoloxydase activity) of transgenic tiger shrimp against pathogenic bacterium V. harveyi. Research using completely randomized design, which consists of two treatments and three replications. Test animals being used were transgenic and non-transgenic shrimp with size, weight 3.93±1.25 g and a total length of 7.59±0.87 cm. Treatments being tested were the injection of bacterium V. harveyi (density of 5x106 cfu/mL) of 0.1 mL/individual on transgenic (A) and non-transgenic shrimp (B). Immune response parameters such as total haemocytes, haemocyte differentiation, and phenoloxydase activity were observed on day 1, 3, and 6 days after challenging. Data were analyzed using t-test by SPSS software. The results showed that the total haemocyte of transgenic shrimp was not significantly different (P>0.05) from non-transgenic shrimp, but haemocyte differentiation and phenoloxydase activity were significantly different (P<0.05) especially on sixth days after being exposed to the bioluminescent bacteria. The study results implied that transgenic shrimp has a better immune response compared than non-transgenic shrimp.

scholarly journals Efektivitas Penggunaan Whole Cell Dari Vibrio alginolyticus Sebagai Vaksin Oral Melalui Artemia Pada Benih Ikan Kerapu Tikus (Chromileptes altivelis) [Effectiveness Of Using Vibrio alginolyticus Whole Cell As Oralvaccine With Artemia To Humpback Grouper Fry (Chromileptes altivelis) ]

2019 ◽  
Vol 2 (2) ◽  
pp. 113
Author(s):  
Titik Desi Sukmawati, Hari Suprapto
Keyword(s):  
Survival Rate ◽  
Dissolved Oxygen ◽  
Water Temperature ◽  
High Mortality ◽  
Oral Vaccine ◽  
Vibrio Alginolyticus ◽  
Water Salinity ◽  
Whole Cell ◽  
Relative Percentage ◽  
Percentage Survival

Abstract Humpback grouper is the one of marine fish which can culture. The major problem of humpback grouper culture is high mortality at short time caused by pathogen infection include virus, bacterial, parasite and fungi. Pathogen infection at humpback grouper fry caused by bacterial from genus Vibrio spp. There was exceeding with vaccination. This research use oral vaccination with whole cell. Vibrio alginolyticus Whole cell as oral vaccine is suggested to prevent high mortality at humpback grouper fry. The purpose of this research is to know the successful about using Vibrio alginolyticus Whole Cell as Oral Vaccine to prevent high mortality of humpback grouper fry (Chromileptes altivelis). The experiment was done June until December 2009 at Gastroenteritis of Laboratory Tropical Disease Centre (TDC) of Airlangga University Surabaya and PT Benur Puteri Situbondo. The methods of this research is descriptive. Major parameter observed in this research was survival rate (%) and Relative Percentage Survival (RPS) of humpback grouper fry. Other parameter observed in this research was water quality include water temperature, pH, water salinity and Dissolved Oxygen (DO). Result of research shows that there are difference of survival rate between fingerling fish vaccination and fingerling fish non vaccination (control). Survival rate of humpback grouper fry which vaccine of Whole Cell 66 % even though survival rate of humpback grouper fry which non vaccine of Whole Cell (control) 46 %. Relative Percentage Survival (RPS) of humpback grouper fry which vaccine of Whole Cell 37 %. The value of RPS was resulted at this research shows low value so that must to perfecting and must to do again research. Water temperature 27-29oC, pH 7,3-7,5, water salinity 31-32 ppt and dissolved oxygen 5-6 ppm.

scholarly journals RESPONS IMUN UDANG WINDU Penaeus monodon TERHADAP VAKSIN dsRNA VP-24 PADA DOSIS BERBEDA

2018 ◽  
Vol 13 (1) ◽  
pp. 77
Author(s):  
Sri Redjeki Hesti Mulyaningrum ◽  
Andi Parenrengi ◽  
Bunga Rante Tampangallo ◽  
Ike Trismawanti
Keyword(s):  
Immune Response ◽  
Survival Rate ◽  
Saline Solution ◽  
Penaeus Monodon ◽  
Challenge Test ◽  
Injection Method ◽  
Tiger Shrimp ◽  
Total Hemocyte Count ◽  
Hemocyte Count

Peningkatan produksi udang windu Penaeus monodon terus diupayakan, salah satunya dengan peningkatan respons imun udang terhadap infeksi penyakit WSSV. Penelitian ini bertujuan untuk mengetahui respons imun udang terhadap pemberian vaksin dsRNA VP-24 pada berbagai dosis. Konstruksi vaksin dsRNA VP-24 dilakukan menggunakan Megascript kit dengan DNA genom VP-24 sebagai template. Vaksinasi dilakukan dengan metode injeksi pada udang windu yang berukuran rata-rata 15,88 ± 3,50 g; dosis vaksin yang diujikan adalah 0,02 µg; 0,2 µg; 2 µg; dan sebagai kontrol adalah udang yang tidak diberi vaksin. Penelitian terdiri atas empat perlakuan dosis vaksin dengan masing-masing dua ulangan dan dipelihara selama lima hari. Uji tantang dilakukan selama enam hari dengan menginjeksi virus WSSV dalam saline solution (1:3 v/v). Pengamatan terhadap sintasan udang windu dilakukan setiap hari, sedangkan penghitungan total hemocyte (THC) dan ProPO diamati pada hari I, III, dan VI setelah diinfeksi WSSV. Pada akhir pengujian dilakukan pengambilan jaringan hepatopankreas untuk analisis histopatologi. Analisis data dilakukan secara statistik dengan analisis ragam (ANOVA). Hasil yang diperoleh memperlihatkan bahwa injeksi vaksin dsRNA VP-24 dengan dosis 0,2 µg memiliki pengaruh yang signifikan terhadap sintasan dan respons imun udang windu (P<0,05). Vaksin dsRNA VP-24 dengan dosis 0,2 µg mampu memberikan sintasan udang windu P. monodon sebesar 65% dan meningkatkan respons imun udang dengan THC (1.550 x 10t sel/mL) dan ProPO (0,042 Abs).One of the efforts to increase the production of tiger shrimp Penaeus monodon is increasing the immune response against WSSV disease. This study aims to evaluate shrimp immune response to dsRNA VP-24 vaccination at various doses. The construction of dsRNA VP-24 vaccine was performed using Megascript kit with the VP-24 DNA genome as a template. The vaccination was done by injection method on shrimp sized 15.88 ± 3.50 g. The tested vaccine doses (treatments) were 0.02 ¼g; 0.2 ¼g; 2 ¼g; and unvaccinated shrimp as the control. The study consisted of four treatments of vaccine doses with two replicates for each treatment. The challenge test was performed by injecting the WSSV virus in saline solution (1:3 v/v). The observation on shrimp survival rate was done daily, while the total hemocyte count (THC) and ProPO observation were performed on the 1st day, 3rd day, and 6th day after WSSV infection. At the end of the experiment, samplings of hepatopancreas for analysis were performed. Data were statistically analyzed using ANOVA. The present study indicated that the injection of 0.2 ¼ g dsRNA VP-24 vaccine had a significant effect on the survival rate and immune response of shrimp (P<0.05). The dose of 0.2 ¼g dsRNA VP-24 had resulted in 65% of survival rate and increased immune response of P. monodon with THC (1,550 x 10t cell/mL) and ProPO (0.042 Abs).

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