scholarly journals Evaluation of haematology analyzer CELL-DYN 3700 SL

2003 ◽  
Vol 3 (2) ◽  
pp. 35-41 ◽  
Author(s):  
Enver Suljević ◽  
Muhamed Fazlić ◽  
Jozo Čorić ◽  
Emina Kiseljaković
Keyword(s):  
Correlation Coefficient ◽  
Sensitivity And Specificity ◽  
Blood Count ◽  
White Blood Count ◽  
Full Blood Count ◽  
Distribution Width ◽  
Medical Laboratories ◽  
Analyzer Cell ◽  
Mean Corpuscular Haemoglobin

Research on the parameters of full blood count and differential white blood count is included in the program of all medical laboratories of primary, secondary and tertiary health care levels. Today, all haematological tests are exclusively performed on the haematology analyzers. Automation of haematology laboratories is a result of the huge requires for haematological test performing, timely issuing of the haematological findings, and possibility of the usage of modern techniques.This work is an evaluation of laser haematology analyzer Cell-Dyn 3700 SL. It investigates the reliability of test results throughout the following parameters: precision, accuracy, sensitivity and specificity of determination methods. It also explores the influence of sample transferring and correlation with haematology analyzer MAXM Retti. Haematology parameters that have been investigated are: white blood cell (WBC), neutrophils (NEU), lymphocytes (LXM), monocytes (MONO), eosinophils (EOS), basophils (BASO), red blood cells (RBC), haemoglobin (HGB), haematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCHC) red cell distribution width (RDW), platelet (PLT), mean platelet volume (MPV), plateletocrit (PCT), and platelet distribution width (PDW).The results confirms that precision of analyzer fulfils the reproducibility of testing parameters: WBC, RBC, HGB, MCV, MCH, MCHC, and PLT. Correlation coefficient values (r) gained throughout the statistical analysis, that is linear regression results obtained throughout the comparison of two analyzers are adequate except for MCHC (r = 0.64), what is in accordance with literature data.Accuracy is tested by haematology analyzer method and microscopic differentiating method. Correlation coefficient results for granulocytes, lymphocytes and monocytes point the accuracy of methods. Sensitivity and specificity parameters fulfil the analytical criteria.It is confirmed that haematology analyzer Cell-Dyn 3700 SL is reliable for the determination of full blood count in everyday work. Analyzer and its program for differential white blood count can be used for the research and separation of normal and pathological blood counts with addition of microscopic methods confirming distribution or morphologic changes of leukocytes.

scholarly journals Acute myeloid leukaemia: an unusual cause of biliary strictures

2019 ◽  
Vol 12 (3) ◽  
pp. e227821
Author(s):  
Adele Beck ◽  
Hannah Hunter ◽  
Simon Jackson ◽  
David Sheridan
Keyword(s):  
Bone Marrow ◽  
Obstructive Jaundice ◽  
Acute Myeloid Leukaemia ◽  
Myeloid Leukaemia ◽  
Blood Count ◽  
Extrahepatic Bile Duct ◽  
White Blood Count ◽  
Full Blood Count ◽  
Significant Past Medical History ◽  
Acute Myeloid

A 17-year-old man with no significant past medical history presented with a 2-week history of worsening jaundice, lethargy, anorexia and progressive right upper quadrant abdominal pain. There were no stigmata of chronic liver disease. Initial investigations were suggestive of cholangitis with large intrahepatic and extrahepatic bile duct strictures but otherwise normal hepatic and splenic appearances. A percutaneous transhepatic cholangiogram with the positioning of drains was performed to alleviate the obstructive jaundice. Within 2 weeks of the first presentation, full blood count revealed a significantly raised white blood count and a subsequent peripheral blood smear and bone marrow were consistent with a diagnosis of acute myeloid leukaemia. Chemotherapy was started after partial improvement of his obstructive jaundice. Complete morphological and cytogenetic remission was obtained 4 weeks after the first cycle of chemotherapy (half dose of daunorubicin and full dose of cytarabine, treated off trial) on control bone marrow. The patient remains in remission.

scholarly journals Are Fibrinogen and Complete Blood Count Parameters Predictive in Incarcerated Abdominal Hernia Repair?

2014 ◽  
Vol 99 (6) ◽  
pp. 723-728 ◽  
Author(s):  
Sahin Kahramanca ◽  
Oskay Kaya ◽  
Gulay Ozgehan ◽  
Hakan Guzel ◽  
Cem Azili ◽  
...  
Keyword(s):  
Hernia Repair ◽  
Blood Count ◽  
Complete Blood Count ◽  
Abdominal Hernia ◽  
White Blood Count ◽  
Morbidity Rate ◽  
Distribution Width ◽  
Diagnostic Role ◽  
Elective Group ◽  
Group B

Abstract Therapeutic delays in cases of external incarcerated hernias typically result in increasing morbidity, mortality, and health expenditures. We investigated the diagnostic role of blood fibrinogen level, white blood count (WBC), mean platelet volume (MPV), and platelet distribution width (PDW) in patients with incarcerated hernia. Two groups, each containing 100 patients, were studied. Group A underwent elective, and group B underwent incarcerated and urgent external hernia repair. We observed high fibrinogen and WBC levels but low MPV and PDW values for patients in group B. Contrary to our expectations, we found lower MPV and PDW values in the complicated group than in the elective group. The morbidity rate and cost burden were higher in group B, and the results were statistically significant. Early operation should be recommended for patients with incarcerated external hernias if their fibrinogen and WBC levels are high.

scholarly journals Reference limits for routine haematological measurements in pre-school and school-children living at an urban area

2004 ◽  
Vol 23 (1) ◽  
pp. 59-64
Author(s):  
Daniela Ardalic ◽  
Slavica Spasic ◽  
Brankica Gulan
Keyword(s):  
Blood Count ◽  
White Blood Count ◽  
Age Group ◽  
Mean Values ◽  
Factors Affecting ◽  
Significant Difference ◽  
Reference Limits ◽  
Laboratory Results ◽  
Analyzer Cell ◽  
Physiological Variations

The aim of this study is to estimate 95% reference limits for eight haematological constituents of blood in order to provide medically reliable evaluation of haematological laboratory results. Blood counts were performed using an automated haematology analyzer (Cell-Dyn 1500) on capillary blood samples from 1918 children (1?16 years old) residing in a region of Novi Beograd. Physiological variations corresponding to age and sex were studied as the most important factors affecting biological variation in haematological constituents of blood. Children were divided in four groups according to age: 1?3 yrs (281 boys and 241 girls), 4? 6 yrs (196 boys and 150 girls), 7?12 yrs (322 boys and 368 girls) and 13 ?16 yrs (174 boys and 186 girls). In this study all investigated parameters were not sex dependent until the age of 13. Boys between ages 13 and 16 years have higher mean values of haemoglobin (143.81 ? 13.40 g/L) and red blood count (4.81 ? 0.49 ? 1012/L), but lower MCV (86.6 ? 5.27 fL) than girls in same age group (136.81 ? 13.42 g/L, 4.57 ? 0.47 ? 1012/L and 88.0 ? 6.96 fL respectively). There was no significant difference according to sex in the oldest group for mean values of haematocrit (boys 0.42 ? 0.049 L/L and girls 4.57 ? 0.47 L/L), MCH (boys 29.9 ? 1.92 pg and girls 30.2 ? 1.96 pg), MCHC (boys 345.9 ? 18.4 g/L and girls 342.2 ? 19.7 g/L), platelet (boys 219.8 ? 49.6 ? 109/L and girls 225.3 ? 59.2 ? 109/L) and white blood count (boys 8.0 ? 2.60 ? 109/L and girls 8.01 ? 2.08 ? 109/L). Haemoglobin level, red blood count, haematocrit, MCV, MCH and MCHC increased with age. Levels of platelet count and white blood count decreased with age.

scholarly journals The Correlation between Complete Blood Count Parameters and Appendix Diameter for the Diagnosis of Acute Appendicitis

Healthcare ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 39 ◽  
Author(s):  
Emin Daldal ◽  
Hasan Dagmura
Keyword(s):  
Acute Appendicitis ◽  
Laboratory Tests ◽  
Blood Count ◽  
Complete Blood Count ◽  
Lymphoid Hyperplasia ◽  
White Blood Count ◽  
C Reactive Protein ◽  
Distribution Width ◽  
Lymphocyte Ratio ◽  
Reactive Protein

Acute appendicitis is one of the most common causes of acute abdominal diseases seen between the ages of 10 and 19, mostly seen in males. The lifetime risk of developing acute appendicitis is 8.6% for males and 6.7% for females. We aimed to investigate the efficacy of the complete blood count parameters, C-reactive protein, and Lymphocyte-C-reactive Protein Ratio laboratory tests in the diagnosis of acute appendicitis, as well as their relationship with appendix diameter. We retrospectively examined all patients who underwent appendectomy between 1 January 2012 and 30 June 2019 in the General Surgery Clinic of Gaziosmanpasa University Faculty of Medicine. Laboratory tests, imaging findings, age, and gender were recorded. Lymphoid hyperplasia is considered as normal appendix—in other words, as negative appendicitis. The distribution of Lymphoid hyperplasia and appendicitis rates were statistically different in the groups formed according to appendix diameter (≤6 and >6 mm) (p < 0.001). We found a significant correlation between appendix diameter and WBC (White blood count), Lymphocyte, Neutrophil, RDW(Red blood cell distribution width), NLR(Neutrophil to lymphocyte ratio), and PLT/L (Platelet to lymphocyte ratio), MPV (Mean platelet volume) and RDW were significantly different in patients with an appendix diameter of ≤6 mm (p = 0.007, p = 0.006, respectively). WBC, Neutrophil, PDW, and NLR values were significantly different between appendicitis and hyperplasia groups in patients with an appendix diameter of >6 mm. The sensitivity of the NLR score (cutoff = 2.6057) in the diagnosis of appendicitis was 86.1% and selectivity was 50% in these patients. Complete blood count parameters evaluation with the clinical findings revealed that NLR is an important parameter that may help the diagnosis of acute appendicitis with an appendix diameter of >6 mm. In patients whose pathological results indicated acute appendicitis but who had a diameter of ≤6 mm, we found an elevated MPV and low RDW values.

scholarly journals Can CRP Levels Predict Infection in Presumptive Aseptic Long Bone Non-Unions? A Prospective Cohort Study

2021 ◽  
Vol 10 (3) ◽  
pp. 425
Author(s):  
Theodoros H. Tosounidis ◽  
Colin Holton ◽  
Vasileios P. Giannoudis ◽  
Nikolaos K. Kanakaris ◽  
Robert M. West ◽  
...  
Keyword(s):  
Revision Surgery ◽  
Blood Count ◽  
Clinical Signs ◽  
Long Bone ◽  
White Blood Count ◽  
Full Blood Count ◽  
Low Grade ◽  
Risk Of Infection ◽  
C Reactive Protein ◽  
Reactive Protein

Nonunion remains a major complication of the management of long bone fractures. The primary aim of the present study was to investigate whether raised levels of C-reactive protein (CRP) and white blood cell count (WBC), in the absence of clinical signs, are correlated with positive intraoperative tissue cultures in presumptive aseptic long-bone nonunions. Infection was classified as positive if any significant growth of microorganisms was observed from bone/tissue samples sent from the theater at the time of revision surgery. Preoperatively all patients were investigated with full blood count, white blood count differential as well as C-reactive protein (CRP). A total of 105 consecutive patients (59 males) were included in the study, with an average age of 46.76 years (range 16–92 years) at the time of nonunion diagnosis. The vast majority were femoral (56) and tibial (37) nonunions. The median time from the index surgical procedure to the time of nonunion diagnosis was 10 months (range 9 months to 10 years). Positive cultures revealed a mixed growth of microorganisms, with coagulase-negative Staphylococcus (56.4%) being the most prevalent microorganism, followed by Staphylococcusaureus (20.5%). Pseudomonas, Methicillin-Resistant Staphylococcus aureus (MRSA), coliforms and micrococcus were present in the remainder of the cases (23.1%). Overall, the risk of infection with normal CRP levels (<10 mg/L) was 21/80 = 0.26. Elevated CRP levels (≥10 mg/L) increased the risk of infection to 0.72. The relative risk given a positive CRP test was RR = 0.72/0.26 = 2.74. Overall, the WBC count was found to be an unreliable marker to predict infection. Solid union was achieved in all cases after an average of 6.5 months (3–24 months) from revision surgery. In patients with presumed aseptic long bone nonunion and normal CRP levels, the risk of underlying low-grade indolent infection can be as high as 26%. Patients should be made aware of this finding, which can complicate their treatment course and outcomes.

scholarly journals The Diagnostic Patterns of Iron Deficiency and Iron Deficiency Anaemia in Children of 6 to 59 Months in Cameroon

2020 ◽  
Vol 2 (4) ◽  
Author(s):  
S. D. Agokeng ◽  
A. L. Njunda ◽  
C. T. Tayou ◽  
C. N. J. Assob
Keyword(s):  
Iron Deficiency ◽  
Mean Corpuscular Volume ◽  
Iron Deficiency Anaemia ◽  
Blood Count ◽  
Ferritin Level ◽  
Full Blood Count ◽  
Corpuscular Volume ◽  
Deficiency Anaemia ◽  
Corpuscular Haemoglobin ◽  
Mean Corpuscular Haemoglobin

A Cross sectional multicentre study was carried out to determine the prevalence of Iron deficiency (ID) and Iron deficiency anaemia (IDA) in children using Ferritin and full blood count. Full blood count was done with Mindray Bc-2800 and Ferritin test with Enzyme Linked Immuno-Sorbent Assay. Authorization was granted by Cameroonian national Ethical Committee. Data were analysed using Statistical Package of Social Sciences 21.0 significant results were considered for P< 0.05. ID varied from 4.2% to 9.6% for cut-offs of 30 and 50μg/L. IDA ranged from 4.2 to 8.5% at these 2 cut-offs. Considering red cells indices, Mean Corpuscular Volume and Mean Corpuscular Haemoglobin blood count currently used should be interpreted cautiously as only Mean Corpuscular Volume and Mean Corpuscular Haemoglobin corroborate with a Ferritin level lower than 30 μg/L.

scholarly journals Comparison of routine hematological indicators of liver and kidney function, blood count and lipid profile in healthy people and stroke patients

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8545
Author(s):  
Xiaofang Cui ◽  
Wei Wei ◽  
Xiao Qin ◽  
Fei Hou ◽  
Jin Zhu ◽  
...  
Keyword(s):  
Renal Function ◽  
Ischemic Stroke ◽  
Liver Function ◽  
Blood Cells ◽  
Blood Count ◽  
Full Blood Count ◽  
Stroke Patients ◽  
Clinical Indicators ◽  
Distribution Width ◽  
Hematological Indicators

Background and methods Stroke has become a major public health problem worldwide. In this article, we carried out statistical analysis, correlation analysis and principal component analysis (PCA) to evaluate the clinical value of routine hematological indicators in early diagnosis of ischemic stroke using R language. Results For the full blood count comparisons, stroke patients had obvious differences in the distribution width of red blood cells (RDW-CV), average distribution width of red blood cells (RDW-SD), mean hemoglobin concentrations, platelet large cell ratio, mean platelet volume and lymphocytes. Patients with ischemic stroke also exhibited different degrees of abnormalities in liver function test. With respect to renal function, stroke patients had obvious changes in uric acid and urea levels. Finally, when comparing the lipid profile, triglyceride concentrations were increased and high-density lipoprotein cholesterol concentrations were reduced in stroke patients. In addition, correlation analysis among these clinical indicators indicated that there were both common characteristics and differences between patients and health controls. Furthermore, the results of PCA indicated that these clinical indicators could distinguish patients from the healthy controls. Conclusion Conventional hematological clinical indicators, such as liver function, renal function, full blood count and lipid concentration profiles highly correlated with the occurrence of ischemic stroke. Therefore, the detection and analyzation of these clinical indicators are of great significance for the prediction of ischemic stroke.

scholarly journals Perforation rate of appendicitis and negative appendectomies in children in Mankweng Hospital

2020 ◽  
Vol 3 (6) ◽  
pp. 991-995
Author(s):  
Mirza Mzu Bhuiyan
Keyword(s):  
Acute Appendicitis ◽  
Blood Count ◽  
Limpopo Province ◽  
White Blood Count ◽  
Full Blood Count ◽  
Midline Incision ◽  
Normal Appendix ◽  
Perforation Rate ◽  
Number Of Children ◽  
Perforated Appendix

Introduction: Appendectomy is a common emergency surgical procedure performed in children. Distances to surgical departments in Limpopo, delays in transportation impose unintentionally conservative initial managements before patients arrive at theatres. Aim: To find out how many children (under of age 15 years) had histologically proven appendicitis,  how many had a normal appendix & what was the outcome of treatment in respect of mortality and wound infections. Method: A retrospective study from January 2004 to December 2007 carried out in the Mankweng Hospital. Cases of laparotomies for appendicitis of all children were retrieved from the hospital archives. Full blood count was done in all patients. Results: The total number of children included in this study was 71, male: 46, female: 25. Histology: acute appendicitis reported in 65 cases, mucocele 1, schistosomiasis 1 and normal appendix in 4 cases. White Blood Cell count was raised in 58 patients (56 in patients with appendicitis, 2 in patients with a normal appendix). Perforated appendix with peritonitis was found in 30 cases. Midline incision was done in 30 patients and local incisions in 41 cases. Wound infection occurred in 18 patients (25.35%). The time of referral to Mankweng hospital: 2 days to more than 7 days from the beginning of symptoms. Conclusions: Many paediatric patients with appendicitis in Limpopo Province came to Mankweng hospital very late; at least 2 days after symptoms began. The perforation rate of appendix was 42% and a normal appendix 5.63% .White Blood Count was raised in 86% in the group of patients with acute appendicitis and in 50% of those with a normal appendix

scholarly journals Impact of various reagents during blood collection from poultry to analyze morpho-biochemical indicators

2021 ◽  
Vol 262 ◽  
pp. 02009
Author(s):  
Vladimir Vertiprakhov ◽  
Alena Grozina ◽  
Vladimir Fisinin ◽  
Natalya Ovchinnikova ◽  
Anatoly Bittirov
Keyword(s):  
Broiler Chickens ◽  
Blood Count ◽  
Sodium Citrate ◽  
Medical Science ◽  
White Blood Count ◽  
Blood Collection ◽  
Blood Samples ◽  
Biochemical Indicators ◽  
The Impact

The work provides experimental data on the impact of anticoagulants (K3 - EDTA, 3.8% sodium citrate and lithium heparin) and coagulation activator on morpho-biochemical indicators in the blood samples of broiler chickens of cross-selection of Agricultural Center “Smena” at the age of 35 days. The studies were carried out with the use of semi-automatic flow analyzer Sinnowa BS-3000P (SINNOWA Medical Science & Technology Co., Ltd, China) and veterinary automatic blood analyzer DF-50 by Dymind Biotech (PRC) with the use of original reagents. The results showed that the most optimal reagent for determination of morphological indicators of the poultry blood was EDTA. The average white blood count (WBC) is lower in blood samples with citrate and heparin in contrast to EDTA by 30.5% and 24.1%, respectively. The average red blood count (RBC) is lower in tubes with sodium citrate (by 37.9 %) and lithium heparin (by 13.8 %) in contrast to K3-EDTA. When the sodium citrate anticoagulant is used, the blood biochemistry results for all positions of the experiment, excluding calcium, are lower than in heparin, and its closest values are lower than in coagulation activator, excluding trypsin activity. That’s why, the most optimal reagents for biochemistry, including for blood enzyme tests, should be considered the tubes with heparin or coagulation activator.

scholarly journals Reference Range of Complete Blood Count in Healthy Term Newborns 1 Week after Birth

2021 ◽  
Vol 9 (A) ◽  
pp. 1565-1569
Author(s):  
Harapan Parlindungan Ringoringo
Keyword(s):  
Birth Weight ◽  
Standard Deviation ◽  
Blood Count ◽  
Reference Range ◽  
Complete Blood Count ◽  
White Blood Count ◽  
Inclusion Criteria ◽  
Term Newborns ◽  
Prospective Analyses

BACKGROUND: Determination of the complete blood count (CBC) and reticulocyte reference range helps diagnose a disease related to changes in erythrocyte indices, white blood count, platelets, and reticulocytes, especially in newborns. AIM: The aim study is to establish a reference range for CBC and reticulocyte in healthy term newborns 1 week after birth. MATERIAL AND METHODS: The study method is prospective analyses of CBC and reticulocyte in term newborns within 1 week after birth. Inclusion criteria are term newborn (gestational age 37–42 weeks) with good tone, breathing, or crying immediately, do not have resuscitation, and the birth weight is ≥2500 g. RESULTS: Two hundred and seventy-seven term newborns met the inclusion criteria. There were 145 (52.35%) male infants and 132 (47.65%) female infants. The reference range of newborns for hemoglobin (Hb) at P2.5–P97.5, P3–P97, P5–P95, and mean ± 2 standard deviation (SD) was 11.70–19.60 g/dL, 11.90–19.57 g/dL, 12.20–19.20 g/dL, and 11.35–19.63 g/dL, respectively. In addition, reference range of male newborns for Hb at P2.5–P97.5, P3–P97, P5–P95, and mean ± 2SD was 12.20–20.14 g/dL, 12.28–19.91 g/dL, 12.63–19.38 g/dL, and 11.47–20.15 g/dL, respectively. The reference range of female newborns for Hb at P2.5–P97.5, P3–P97, P5–P95, and mean ± 2 SD was 11.37–19.20 g/dL, 11.50–19.20 g/dL, 11.80–19.20 g/dL, and 11.37–18.93 g/dL, respectively. CONCLUSION: The reference range of CBC and reticulocyte for healthy term newborns, male newborns, and female newborns in this study can be used as a benchmark.

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