scholarly journals Super-resolution for 2.5D height data of microstructured surfaces using the vdsr network

2020 ◽  
Vol 238 ◽  
pp. 06014
Author(s):  
Stefan Siemens ◽  
Markus Kästner ◽  
Eduard Reithmeier
Keyword(s):  
Laser Scanning ◽  
Signal To Noise Ratio ◽  
Similarity Index ◽  
Super Resolution ◽  
Structural Similarity ◽  
Confocal Laser Scanning Microscope ◽  
Confocal Laser ◽  
Microstructured Surfaces ◽  
Resolution Imaging ◽  
Height Data

In this work super-resolution imaging is used to enhance 2.5D height data of thermal sprayed Al2O3 ceramics with stochastically microstructured surfaces. The data is obtained by means of a confocal laser scanning microscope. By implementing and training a Very Deep Super-Resolution neural network to generate residual images an improvement of the peak signal-to-noise ratio and structural similarity index can be observed when compared to classic interpolation methods.

3-D imaging of cells using a confocal laser scanning microscope and digital image processing

1988 ◽  
Vol 46 ◽  
pp. 96-97
Author(s):  
Thomas M. Jovin ◽  
Michel Robert-Nicoud ◽  
Donna J. Arndt-Jovin ◽  
Thorsten Schormann
Keyword(s):  
Laser Scanning ◽  
Confocal Laser Scanning Microscope ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Scanning Microscope ◽  
Laser Scanning Microscope ◽  
Biochemical Processes ◽  
Adjacent Structures

Light microscopic techniques for visualizing biomolecules and biochemical processes in situ have become indispensable in studies concerning the structural organization of supramolecular assemblies in cells and of processes during the cell cycle, transformation, differentiation, and development. Confocal laser scanning microscopy offers a number of advantages for the in situ localization and quantitation of fluorescence labeled targets and probes: (i) rejection of interfering signals emanating from out-of-focus and adjacent structures, allowing the “optical sectioning” of the specimen and 3-D reconstruction without time consuming deconvolution; (ii) increased spatial resolution; (iii) electronic control of contrast and magnification; (iv) simultanous imaging of the specimen by optical phenomena based on incident, scattered, emitted, and transmitted light; and (v) simultanous use of different fluorescent probes and types of detectors.We currently use a confocal laser scanning microscope CLSM (Zeiss, Oberkochen) equipped with 3-laser excitation (u.v - visible) and confocal optics in the fluorescence mode, as well as a computer-controlled X-Y-Z scanning stage with 0.1 μ resolution.

3-Dimensional immunolabeling and in situ hybridization detection using fluorescence photooxidation and intermediate-voltage Electron Microscopy

1994 ◽  
Vol 52 ◽  
pp. 164-165
Author(s):  
Thomas J. Deerinck ◽  
Maryann E. Martone ◽  
Varda Lev-Ram ◽  
David P. L. Green ◽  
Roger Y. Tsien ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Reactive Oxygen ◽  
Confocal Laser Scanning Microscope ◽  
Fluorescent Dye ◽  
Confocal Laser ◽  
3 Dimensional ◽  
Voltage Electron ◽  
Dimensional Distribution ◽  
Electron Microscopes ◽  
New Generation

The confocal laser scanning microscope has become a powerful tool in the study of the 3-dimensional distribution of proteins and specific nucleic acid sequences in cells and tissues. This is also proving to be true for a new generation of high contrast intermediate voltage electron microscopes (IVEM). Until recently, the number of labeling techniques that could be employed to allow examination of the same sample with both confocal and IVEM was rather limited. One method that can be used to take full advantage of these two technologies is fluorescence photooxidation. Specimens are labeled by a fluorescent dye and viewed with confocal microscopy followed by fluorescence photooxidation of diaminobenzidine (DAB). In this technique, a fluorescent dye is used to photooxidize DAB into an osmiophilic reaction product that can be subsequently visualized with the electron microscope. The precise reaction mechanism by which the photooxidation occurs is not known but evidence suggests that the radiationless transfer of energy from the excited-state dye molecule undergoing the phenomenon of intersystem crossing leads to the formation of reactive oxygen species such as singlet oxygen. It is this reactive oxygen that is likely crucial in the photooxidation of DAB.

A confocal laser scanning microscope for fluorescence and reflection at video rates

1989 ◽  
Vol 47 ◽  
pp. 134-135
Author(s):  
P.M. Houpt ◽  
A. Draaijer
Keyword(s):  
Light Source ◽  
Laser Scanning ◽  
Focal Point ◽  
Confocal Laser Scanning Microscope ◽  
Confocal Laser ◽  
Point Light Source ◽  
Volume Of Interest ◽  
Ion Laser ◽  
Point Light ◽  
Point Detector

In confocal microscopy, the object is scanned by the coinciding focal points (confocal) of a point light source and a point detector both focused on a certain plane in the object. Only light coming from the focal point is detected and, even more important, out-of-focus light is rejected.This makes it possible to slice up optically the ‘volume of interest’ in the object by moving it axially while scanning the focused point light source (X-Y) laterally. The successive confocal sections can be stored in a computer and used to reconstruct the object in a 3D image display.The instrument described is able to scan the object laterally with an Ar ion laser (488 nm) at video rates. The image of one confocal section of an object can be displayed within 40 milliseconds (1000 х 1000 pixels). The time to record the total information within the ‘volume of interest’ normally depends on the number of slices needed to cover it, but rarely exceeds a few seconds.

Use of confocal laser scanning microscopy and a computer model to understand ink cavitation and filamentation

TAPPI Journal ◽  
2010 ◽  
Vol 9 (10) ◽  
pp. 7-15
Author(s):  
HANNA KOIVULA ◽  
DOUGLAS BOUSFIELD ◽  
MARTTI TOIVAKKA
Keyword(s):  
Laser Scanning ◽  
Confocal Laser Scanning Microscope ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Print Quality ◽  
Length Scale ◽  
Offset Printing ◽  
Significant Difference ◽  
Printing Speed

In the offset printing process, ink film splitting has an important impact on formation of ink filaments. The filament size and its distribution influence the leveling of ink and hence affect ink setting and the print quality. However, ink filaments are difficult to image due to their short lifetime and fine length scale. Due to this difficulty, limited work has been reported on the parameters that influence filament size and methods to characterize it. We imaged ink filament remains and quantified some of their characteristics by changing printing speed, ink amount, and fountain solution type. Printed samples were prepared using a laboratory printability tester with varying ink levels and operating settings. Rhodamine B dye was incorporated into fountain solutions to aid in the detection of the filaments. The prints were then imaged with a confocal laser scanning microscope (CLSM) and images were further analyzed for their surface topography. Modeling of the pressure pulses in the printing nip was included to better understand the mechanism of filament formation and the origin of filament length scale. Printing speed and ink amount changed the size distribution of the observed filament remains. There was no significant difference between fountain solutions with or without isopropyl alcohol on the observed patterns of the filament remains.

ANALISIS SENSITIVITAS VIDEO MPEG-4 BERDASARKAN STRUKTUR FRAME PADA TRANSMISI DVB-T

2020 ◽  
Vol 25 (2) ◽  
pp. 86-97
Author(s):  
Sandy Suryo Prayogo ◽  
Tubagus Maulana Kusuma
Keyword(s):  
Deep Learning ◽  
Bit Error Rate ◽  
Error Rate ◽  
Signal To Noise Ratio ◽  
Similarity Index ◽  
Structural Similarity ◽  
Signal To Noise ◽  
Structural Similarity Index ◽  
Noise Ratio

DVB merupakan standar transmisi televisi digital yang paling banyak digunakan saat ini. Unsur terpenting dari suatu proses transmisi adalah kualitas gambar dari video yang diterima setelah melalui proses transimisi tersebut. Banyak faktor yang dapat mempengaruhi kualitas dari suatu gambar, salah satunya adalah struktur frame dari video. Pada tulisan ini dilakukan pengujian sensitifitas video MPEG-4 berdasarkan struktur frame pada transmisi DVB-T. Pengujian dilakukan menggunakan simulasi matlab dan simulink. Digunakan juga ffmpeg untuk menyediakan format dan pengaturan video akan disimulasikan. Variabel yang diubah dari video adalah bitrate dan juga group-of-pictures (GOP), sedangkan variabel yang diubah dari transmisi DVB-T adalah signal-to-noise-ratio (SNR) pada kanal AWGN di antara pengirim (Tx) dan penerima (Rx). Hasil yang diperoleh dari percobaan berupa kualitas rata-rata gambar pada video yang diukur menggunakan metode pengukuran structural-similarity-index (SSIM). Dilakukan juga pengukuran terhadap jumlah bit-error-rate BER pada bitstream DVB-T. Percobaan yang dilakukan dapat menunjukkan seberapa besar sensitifitas bitrate dan GOP dari video pada transmisi DVB-T dengan kesimpulan semakin besar bitrate maka akan semakin buruk nilai kualitas gambarnya, dan semakin kecil nilai GOP maka akan semakin baik nilai kualitasnya. Penilitian diharapkan dapat dikembangkan menggunakan deep learning untuk memperoleh frame struktur yang tepat di kondisi-kondisi tertentu dalam proses transmisi televisi digital.

scholarly journals 3D super-resolved imaging in live cells using sub-diffractive plasmonic localization of hybrid nanopillar arrays

Nanophotonics ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 2847-2859
Author(s):  
Soojung Kim ◽  
Hyerin Song ◽  
Heesang Ahn ◽  
Seung Won Jun ◽  
Seungchul Kim ◽  
...  
Keyword(s):  
Signal To Noise Ratio ◽  
Imaging Techniques ◽  
Optical Loss ◽  
Super Resolution ◽  
Living Cells ◽  
Live Cells ◽  
Complex Biological System ◽  
Observation Volume ◽  
Resolution Imaging ◽  
Nanopillar Arrays

AbstractAnalysing dynamics of a single biomolecule using high-resolution imaging techniques has been had significant attentions to understand complex biological system. Among the many approaches, vertical nanopillar arrays in contact with the inside of cells have been reported as a one of useful imaging applications since an observation volume can be confined down to few-tens nanometre theoretically. However, the nanopillars experimentally are not able to obtain super-resolution imaging because their evanescent waves generate a high optical loss and a low signal-to-noise ratio. Also, conventional nanopillars have a limitation to yield 3D information because they do not concern field localization in z-axis. Here, we developed novel hybrid nanopillar arrays (HNPs) that consist of SiO2 nanopillars terminated with gold nanodisks, allowing extreme light localization. The electromagnetic field profiles of HNPs are obtained through simulations and imaging resolution of cell membrane and biomolecules in living cells are tested using one-photon and 3D multiphoton fluorescence microscopy, respectively. Consequently, HNPs present approximately 25 times enhanced intensity compared to controls and obtained an axial and lateral resolution of 110 and 210 nm of the intensities of fluorophores conjugated with biomolecules transported in living cells. These structures can be a great platform to analyse complex intracellular environment.

Joint Encryption and Compression-Based Watermarking Technique for Security of Digital Documents

2021 ◽  
Vol 21 (1) ◽  
pp. 1-20
Author(s):  
A. K. Singh ◽  
S. Thakur ◽  
Alireza Jolfaei ◽  
Gautam Srivastava ◽  
MD. Elhoseny ◽  
...  
Keyword(s):  
Signal To Noise Ratio ◽  
Similarity Index ◽  
Structural Similarity ◽  
Digital Data ◽  
The Internet ◽  
Discrete Wavelet ◽  
Compression Performance ◽  
Strong Compression ◽  
Multiple Watermarks ◽  
Value Decomposition

Recently, due to the increase in popularity of the Internet, the problem of digital data security over the Internet is increasing at a phenomenal rate. Watermarking is used for various notable applications to secure digital data from unauthorized individuals. To achieve this, in this article, we propose a joint encryption then-compression based watermarking technique for digital document security. This technique offers a tool for confidentiality, copyright protection, and strong compression performance of the system. The proposed method involves three major steps as follows: (1) embedding of multiple watermarks through non-sub-sampled contourlet transform, redundant discrete wavelet transform, and singular value decomposition; (2) encryption and compression via SHA-256 and Lempel Ziv Welch (LZW), respectively; and (3) extraction/recovery of multiple watermarks from the possibly distorted cover image. The performance estimations are carried out on various images at different attacks, and the efficiency of the system is determined in terms of peak signal-to-noise ratio (PSNR) and normalized correlation (NC), structural similarity index measure (SSIM), number of changing pixel rate (NPCR), unified averaged changed intensity (UACI), and compression ratio (CR). Furthermore, the comparative analysis of the proposed system with similar schemes indicates its superiority to them.

scholarly journals Nonlinear absorption and scattering of a single plasmonic nanostructure characterized by x-scan technique

2019 ◽  
Vol 10 ◽  
pp. 2182-2191 ◽  
Author(s):  
Tushar C Jagadale ◽  
Dhanya S Murali ◽  
Shi-Wei Chu
Keyword(s):  
Laser Scanning ◽  
Detection System ◽  
Nonlinear Behavior ◽  
Optical Nonlinearity ◽  
Research Area ◽  
Confocal Laser Scanning Microscope ◽  
Confocal Laser ◽  
Thin Sample ◽  
Channel Detection ◽  
Novel Method

Nonlinear nanoplasmonics is a largely unexplored research area that paves the way for many exciting applications, such as nanolasers, nanoantennas, and nanomodulators. In the field of nonlinear nanoplasmonics, it is highly desirable to characterize the nonlinearity of the optical absorption and scattering of single nanostructures. Currently, the common method to quantify optical nonlinearity is the z-scan technique, which yields real and imaginary parts of the permittivity by moving a thin sample with a laser beam. However, z-scan typically works with thin films, and thus acquires nonlinear responses from ensembles of nanostructures, not from single ones. In this work, we present an x-scan technique that is based on a confocal laser scanning microscope equipped with forward and backward detectors. The two-channel detection offers the simultaneous quantification for the nonlinear behavior of scattering, absorption and total attenuation by a single nanostructure. At low excitation intensities, both scattering and absorption responses are linear, thus confirming the linearity of the detection system. At high excitation intensities, we found that the nonlinear response can be derived directly from the point spread function of the x-scan images. Exceptionally large nonlinearities of both scattering and absorption are unraveled simultaneously for the first time. The present study not only provides a novel method for characterizing nonlinearity of a single nanostructure, but also reports surprisingly large plasmonic nonlinearities.

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