Positive Interaction of Thyme (Red) Essential Oil with Human Polymorphonuclear Granulocytes in Eradicating Intracellular Candida albicans

To evaluate the leaf volatile constituents of essential oil of Coleus zeylanicus and evaluate their anti-oxidant and anti-fungal activity. The Chemical composition of Coleus zeylanicus essential oil was determined using GC-MS and FT-IR analytical techniques. The antioxidant activity was evaluated using DPPH scavenging assay. The anti-fungal effect was tested against two potential pathogenic fungal strains - Candida albicans and Malassezia furfur using agar well diffusion method. The essential oil was profiled by the presence of sesquiterpene hydrocarbons 90.67% of their total composition followed by oxygenated monoterpenes and monoterpene hydrocarbons as 5.3% and 2.1% respectively. The GC-MS results showed 14 compounds from Coleus zeylanicus leaf EO representing 98.07% of the total oil composition. The major component was identified as a-Gurjunene (35.94%), a-bisabolol (10.82%) and G-selinene (4.26%). EO showed remarkable antioxidant activity values of IC50 = 59.78± 3.21µg/ml by DPPH scavenging assay. The essential oil showed interesting anti-fungal effects against two pathogenic fungal strains. The most sensible strains to Coleus zeylanicus EO was Malassezia furfur (32.00±0.50mm) compared to that of Candida albicans (15.00±1.25mm). Hence, Coleus zeylanicus EO has potential application against fungal infection and oxidative stress-related diseases. However, further investigations are necessary to isolate and investigate the action mechanism of these bioactive compounds.

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Antifungal Activity of the Essential Oil of Echinops kebericho Mesfin: An In Vitro Study

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/3101324 ◽

2020 ◽

Vol 2020 ◽

pp. 1-7

Author(s):

Tamirat Bekele Beressa ◽

Serawit Deyno ◽

Paul E. Alele

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Essential Oil ◽

Cryptococcus Neoformans ◽

Antioxidant Properties ◽

Pathogenic Fungi ◽

Diffusion Method ◽

Potential Candidate ◽

Standard Drug

Background. Echinops kebericho is an endemic medicinal plant in Ethiopia widely used in the treatment of infectious and noninfectious diseases. Essential oils are known for their antibacterial, antifungal, antiviral, insecticidal, and antioxidant properties. This study evaluated the antifungal activity of essential oil from E. kebericho against four common pathogenic fungi and two standard strains. Methods. The essential oil was obtained by hydrodistillation. The antifungal screening was done by agar well diffusion method. Minimal inhibitory concentrations (MICs) were determined by broth microdilution. Minimal fungicidal concentrations (MFCs) were determined by subculturing fungal strains with no visible growth onto a Sabouraud dextrose agar (SDA) plate. Results. Candida albicans and Cryptococcus neoformans were highly sensitive while Aspergillus flavus did not show sensitivity up to 1 mg/ml of essential oil; MICs ranged from 0.083 mg/ml to 0.208 mg/ml. Concentration and fungal species showed significant dose-dependent associations ( p < 0.0001 ) with antifungal activity. The MICs of essential oil were comparable to those of the standard drug (fluconazole) against C. glabrata and C. krusei. The lowest MFC of the essential oil was observed against Candida parapsilosis (0.145 mg/ml) while the highest MFC was against Candida krusei (0.667 mg/ml). Conclusion. Echinops kebericho essential oil showed noteworthy antifungal activity against Cryptococcus neoformans, Candida albicans, and Candida glabrata and could be a potential candidate for further antifungal drug development.

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Chemical Composition and Preliminary Antimicrobial Activity of the Hydroxylated Sesquiterpenes in the Essential Oil from Piper barbatum Kunth Leaves

Plants ◽

10.3390/plants9020211 ◽

2020 ◽

Vol 9 (2) ◽

pp. 211 ◽

Cited By ~ 3

Author(s):

Paco Noriega ◽

José Ballesteros ◽

Alejandra De la Cruz ◽

Tatiana Veloz

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Chemical Composition ◽

Essential Oil ◽

Forest Ecosystem ◽

Raw Materials ◽

The Andes ◽

Ancestral Knowledge ◽

Antifungal Potential

This study evaluates the antimicrobial and antifungal potential of the essential oil extracted from a species located in the Andes of Ecuador, Piper barbatum Kunth, known as “cordoncillo” or “allupa”, used by the Quichua people as an antibacterial plant for washing female genitalia in cases of infection. The most abundant molecules in the essential oil were: α- phellandrene (43.16%), limonene (7.04%); some oxygenated sesquiterpenes such as: trans-sesquisabinene hydrate (8.23%), elemol (7.21%) and others. The evaluation of antimicrobial activity showed activity in all the strains analyzed; however, those in which MIC values are considered to be very strong (less than 500 µg/mL) are: Staphylococcus aureus 264 µg/mL, Streptococcus mutans 132 µg/mL, Candida albicans 132 µg/mL and Candida tropicalis 264 µg/mL. Antimicrobial bioautography defines which molecules are responsible for the activity; thus, it was possible to establish the chromatographic regions of = 0.02 and Rf = 0.04, as those with active molecules. It was established that 4 hydroxylated sesquiterpene molecules are involved: elemol (7.21%), trans-sesquisabinene hydrate (8.23%), β–eudesmol (3.49%) and 10-epi-γ-eudesmol (1.07%); the last two being the most active. The aim of this manuscript is to analyze both the ancestral knowledge of the Quichua people of Ecuador, and the chemical-biodiversity of the Andean forest ecosystem, in order to provide new raw materials of pharmaceutical interest.

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Satureja khuzistanica Jamzad essential oil and its anti-candidal activities against clinical isolates of Candida albicans isolated from women with candidiasis

Infectio ◽

10.22354/in.v23i1.750 ◽

2018 ◽

Vol 23 (1) ◽

pp. 16 ◽

Cited By ~ 1

Author(s):

Mohaddese Mahboubi ◽

Bahareh Attaran

Keyword(s):

Candida Albicans ◽

Essential Oil ◽

Germ Tube ◽

Folk Medicine ◽

Tube Formation ◽

Clinical Isolates ◽

Analgesic Agent ◽

Aerial Parts ◽

Main Component ◽

Cytoplasmic Structures

Satureja khuzistanica Jamzad is known as antiseptic and analgesic agent in folk medicine. The aim of this investigation was to evaluate the anti-candidal activity of S. khuzistanica aerial parts essential oil against clinical isolates of Candida albicans, which were isolated from women with chronic recurrent candidiasis. For this purpose, the chemical composition of hydro-distilled essential oil was determined by GC and GC-MS analysis. Then, the anti-candidal activity of essential oil and its main component (carvacrol) were determined. Carvacrol (94.1%) was the main component of essential oil, followed by β-bisabolene, p-cymene and γ-terpinene. S. khuzistanica essential oil had strong anti-candidal activity against clinical isolates of C. albicans via inhibition of germ tube formation and induction the huge punctures in the cytoplasmic structures. The cell membranes were intact in presence of essential oil or carvacrol. S. khuzistanica essential oil as the main source of carvacrol can be used for treatment of C. albicans related infections.

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Mexican oregano (Lippia graveolens) essential oil-in-water emulsions: impact of emulsifier type on the antifungal activity of Candida albicans

Food Science and Biotechnology ◽

10.1007/s10068-018-0499-6 ◽

2018 ◽

Vol 28 (2) ◽

pp. 441-448 ◽

Cited By ~ 3

Author(s):

S. E. Herrera-Rodríguez ◽

R. J. López-Rivera ◽

E. García-Márquez ◽

M. Estarrón-Espinosa ◽

H. Espinosa-Andrews

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Essential Oil ◽

Oil In Water

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In vitro Antimicrobial Properties and Chemical Composition of Santolina chamaecyparissus Essential Oil from Algeria

Natural Product Communications ◽

10.1177/1934578x1200700735 ◽

2012 ◽

Vol 7 (7) ◽

pp. 1934578X1200700 ◽

Cited By ~ 2

Author(s):

Samah Djeddi ◽

Khadidja Djebile ◽

Ghania Hadjbourega ◽

Zoubida Achour ◽

Catherine Argyropoulou ◽

...

Keyword(s):

Antimicrobial Activity ◽

Candida Albicans ◽

Chemical Composition ◽

Essential Oil ◽

Antimicrobial Properties ◽

Klebsiella Pneumonia ◽

Aerial Parts

The chemical composition of the essential oil obtained from the aerial parts of Santolina chamaecyparissus L., growing in Algeria, was investigated by GC-MS analyses. A total of 36 compounds were identified, accounting for 91.7% of the essential oil obtained. Camphor (31.1%) and cubenol (17.0%) were the predominant compounds. The potential of the antimicrobial activity was also investigated and the tested sample proved to be very active against Klebsiella pneumonia and Candida albicans (34.1 ± 0.02 mm and 35.0 ± 0.01 mm, respectively). Transverse sections of the leaf and stem of the plant suggest that the essential oil is localized in endogenous and exogenous sites.

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Determination of the effects of cinnamon bark fractions on Candida albicans and oral epithelial cells

BMC Complementary and Alternative Medicine ◽

10.1186/s12906-019-2730-2 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 6

Author(s):

Marie-Pier Veilleux ◽

Daniel Grenier

Keyword(s):

Candida Albicans ◽

Epithelial Cells ◽

Essential Oil ◽

Biofilm Formation ◽

Epithelial Barrier ◽

Cinnamon Oil ◽

Cinnamon Bark ◽

Oral Epithelial Cells ◽

Oral Epithelial

Abstract Background Candida albicans is an opportunistic pathogen that causes oral candidiasis and denture stomatitis. It has also been reported to infect oral mucositis lesions in patients who suffer from cancer affecting the head and neck and who receive chemotherapy and radiotherapy treatments. This study aimed to investigate the effects of two cinnamon bark fractions, i.e., an essential oil and an aqueous extract enriched in proanthocyanidins (Cinnulin PF®) on growth, biofilm formation, and adherence properties of C. albicans as well as on oral epithelial cells (barrier integrity, inflammatory response). Methods A microplate dilution assay was used to determine antifungal and anti-biofilm properties. A fluorescent assay was used to determine C. albicans adherence to oral epithelial cells. Cytotoxicity toward oral epithelial cells was assessed by determination of cell metabolic activity. Tight junction integrity of gingival keratinocytes was assessed by determination of transepithelial electrical resistance. IL-6 and IL-8 secretion by TNFα-stimulated oral epithelial cells was quantified by ELISA. Results While Cinnulin PF® did not reduce C. albicans growth, the cinnamon bark oil exhibited high antifungal activity with minimum inhibitory concentrations and minimum fungicidal concentrations in the range of 0.039 to 0.078%. The cinnamon oil was also active against a pre-formed C. albicans biofilm. Interestingly, Cinnulin PF® prevented biofilm formation by C. albicans and attenuated its adherence to oral epithelial cells. At their effective concentrations, the cinnamon oil and the Cinnulin PF® displayed no significant cytotoxicity against oral epithelial cells. In an in vitro model, both cinnamon fractions reinforced the integrity of the oral epithelial barrier. Lastly, Cinnulin PF® inhibited the secretion of interleukin-6 and interleukin-8 by oral epithelial cells stimulated with TNF-α. Conclusion By their ability to attenuate growth, biofilm formation and adherence property of C. albicans, to reinforce the epithelial barrier function, and to exert anti-inflammatory properties the two cinnamon fractions (essential oil, Cinnulin PF®) investigated in the present study may be promising agents for treating oral infections involving C. albicans.

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Essential Oil Composition and Antimicrobial Activity of Aerial Parts and Ripe Fruits of Echinophora spinosa (Apiaceae) from Italy

Natural Product Communications ◽

10.1177/1934578x1300800431 ◽

2013 ◽

Vol 8 (4) ◽

pp. 1934578X1300800

Author(s):

Daniele Fraternale ◽

Salvatore Genovese ◽

Donata Ricci

Keyword(s):

Antimicrobial Activity ◽

Candida Albicans ◽

Chemical Composition ◽

Essential Oil ◽

Intestinal Microflora ◽

Central Italy ◽

Essential Oil Composition ◽

Oil Composition ◽

Aerial Parts ◽

Eubacterium Limosum

The chemical composition and antimicrobial activity of the essential oils obtained from the flowering aerial parts and ripe fruits of Echinophora spinosa L. (Apiaceae) from central Italy were analyzed by GC/MS. The major constituents of the oil from the aerial parts were β-phellandrene (34.7%), myristicin (16.5%), δ3-carene (12.6%), α-pinene (6.7%) and α-phellandrene (6.2%), and of the oil from the ripe fruits p-cymene (50.2%), myristicin (15.3%), α-pinene (15.1%) and α-phellandrene (8.1%). The two oils showed good antimicrobial activity against Clostridium difficile, C perfringens, Enterococcus faecalis, Eubacterium limosum, Peptostreptococcus anaerobius and Candida albicans with MIC values respectively of 0.25, 0.25, 0.25, 0.25, 2.25, and 0.50%, v/v, and 0.13, 0.13, 0.13, 0.13, 2.25, 0.50%, v/v, for aerial parts and ripe fruits respectively. A less significant antimicrobial activity against bifidobacteria and lactobacilli, very important in the intestinal microflora, was also detected, with MIC values higher than 4.0%, v/v.

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