scholarly journals Hepatoprotective Screening of Seriphidium kurramense (Qazilb.) Y.R. Ling

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Maroof Ali ◽  
Hidayat Hussain ◽  
Amjad Hussain ◽  
Abdur Rauf ◽  
Wahid Hussain ◽  
...  
Keyword(s):  
Dna Damage ◽  
Liver Toxicity ◽  
Methanolic Extract ◽  
Therapeutic Potential ◽  
Hepatoprotective Activity ◽  
Nitrate Content ◽  
Serum Samples ◽  
Morphological Alterations ◽  
Ccl4 Administration ◽  
Altered Liver

Investigation on medicinal plants’ therapeutic potential has gained substantial importance in the discovery of novel effective and safe therapeutic agents. The present study is aimed at investigating the hepatoprotective potential of Seriphidium kurramense methanolic extract (SKM) against carbon tetrachloride- (CCl4-) induced hepatotoxicity in rats. S. kurramense is one of the most imperative plants for its various pharmacological activities. Therefore, this study was aimed at evaluating the hepatoprotective potential against CCl4-induced liver toxicity. The serum samples were analyzed for alanine aminotransferase (ALT) and aspartate aminotransferase (AST) together with the oxidative stress mediator levels as nitric oxide (NO), malondialdehyde (MDA), glutathione (GSH), reduced glutathione (GSH), and superoxide dismutase (SOD) as well as peroxidation and H2O2 activity. CCl4 administration resulted in an elevated free radical generation, altered liver marker (AST and ALT) enzymes, reduced antioxidant enzyme, and increased DNA damage. Methanolic extract of S. kurramense decreased CCl4-induced hepatotoxicity by increasing the antioxidant status and reducing H2O2 and nitrate content generation as well as reducing DNA damage. Additionally, SKM reversed the morphological alterations induced by CCl4 in the SKM-treated groups. These results demonstrated that SKM displayed hepatoprotective activity against CCl4-induced hepatic damage in experimental rats.

scholarly journals Methanolic Extract of Dill Leaves Inhibits AGEs Formation and Shows Potential Hepatoprotective Effects in CCl4 Induced Liver Toxicity in Rat

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Ebrahim Abbasi Oshaghi ◽  
Iraj Khodadadi ◽  
Fatemeh Mirzaei ◽  
Mozafar Khazaei ◽  
Heidar Tavilani ◽  
...  
Keyword(s):  
Diabetes Complications ◽  
Liver Toxicity ◽  
Methanolic Extract ◽  
Antioxidant Properties ◽  
Hepatoprotective Activity ◽  
Protein Carbonyl ◽  
Anethum Graveolens ◽  
Treatment Of Diabetes ◽  
Hepatoprotective Effects ◽  
Protein Fragmentation

The research was aimed at evaluating the antiglycation, antioxidant, and hepatoprotective properties of methanolic extract of Anethum graveolens (dill). The antioxidant properties, photochemical characteristics, and antiglycation effects of dill extract were measured. Carbon tetrachloride-induced hepatotoxic rats were used to show the hepatoprotective activity of dill leaves. Different concentration of dill extract (0.032, 0.065, 0.125, 0.25, 0.5, and 1 mg/mL) showed potential antioxidant ability. The extract of dill leaves significantly reduced AGEs formation and also fructosamine and protein carbonyl levels in rats’ liver. Thiol groups’ oxidation, amyloid cross-β, and protein fragmentation (P<0.001) significantly reduced in treated rats. Liver damage markers significantly reduced in dill-treated animals (P<0.05). Dill with potential antioxidant, antiglycation, and hepatoprotective effects can be suggested for treatment of diabetes complications.

scholarly journals OXIDATIVE DNA DAMAGE PROTECTIVE AND HEPATOPROTECTIVE ACTIVITY OF TERMI-NALIA BELLERICA (BAHEDA)

2018 ◽  
Vol 10 (1) ◽  
pp. 132 ◽  
Author(s):  
Rasna Gupta ◽  
Ram Lakhan Singh ◽  
Andab Pant
Keyword(s):  
Dna Damage ◽  
Hepg2 Cells ◽  
Oxidative Dna Damage ◽  
Therapeutic Potential ◽  
Hepatoprotective Activity ◽  
Dependent Manner ◽  
Fruit Pulp ◽  
Protective Activity ◽  
Trypan Blue Exclusion ◽  
Dna Damage Protective

Objective: To investigate the ethanolic extracts of Terminalia bellerica for its in vitro DNA damage protective activity against hydroxyl radical (OH•) and hepatoprotective activity against CCl4 induced toxicity in HepG2 liver cells.Methods: The DNA damage assay was performed using supercoiled pBR322 plasmid DNA with Fenton’s reagent. Protection of human liver-derived HepG2 cells against CCl4 induced damage was determined by trypan blue exclusion assay and Tetrazolium bromide salt MTT assay. Liver cells were pre-exposed to 0.5 µmol/ml of CCl4 for 60 min at room temperature and screening assay was carried out in HepG2 cells to evaluate the cytotoxicity of tested extracts with concentration 0.001 to 100 µg/ml.Results: The low dose (0.001-0.1 µg/ml) of leaf and bark has an effect on the percentage viability (101±4.04) and 97±4.04) for leaf and bark extracts respectively) of HepG2 cells. Whereas, the percentage cell viability 99±2.89 and 103±4.04 were found for fruit pulp and seed extracts respectively. The uniform DNA damage protective activity was observed in a dose-dependent manner for leaf, fruit pulp, seed and bark extracts of T. bellerica. Conclusion: Leaf and bark of T. bellerica exhibit 10 fold less toxicity compared to fruit pulp and seed during CCl4 exposure on HepG2 cells suggesting that leaf and bark has more therapeutic potential against hepatotoxicity.

scholarly journals Hepatoprotective Activity of Acampe praemorsa

2021 ◽  
pp. 162-168
Author(s):  
Praveen Kumar Uppala ◽  
G. S. N. Koteswara Rao ◽  
A. Rajasekhar Reddy ◽  
K. Umasankar ◽  
C. Joshitha ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Liver Toxicity ◽  
Hepatoprotective Activity ◽  
Alcoholic Extract ◽  
Standard Drug ◽  
Traditional Medicines ◽  
Medicinal Use ◽  
Phytochemical Compounds ◽  
Study Support ◽  
Altered Liver

Introduction: Liver is one of major functional organ in body, its damage can alter body metabolisms and other organs’ function. So, it is very important to maintain the healthy liver. Now a days, different chemicals and inadequate use of medicines are causing liver impairments including alcohol consumption. There is a need to identify safe hepatoprotective drugs against liver diseases from different natural resources including medicinal plants. Several medicinal plants have been using in traditional medicines against several diseases including liver disease and many of them are not scientifically proven. So, the current study was aimed to evaluate hepatoprotective nature of Acampe praemorsa. Methodology: The hepatoprotective activity of A. praemorsa was carried on ethanol-induced liver toxicity on albino wistar rats by evaluating the levels of liver biomarker enzymes such as aspartate aminotransferase (AST), Alanine transaminase (ALT), Alkaline phosphatase (ALP), Total protein (T.ptn), and Total bilirubin (T.Bil). Results: The extracts of Acampe praemorsa were found to be safe at different doses as there were no mortality and physio-psychological changes observed in toxicity study. The extracts of Acampe praemorsa has showed dose dependent hepatoprotective activity on controlling the altered liver biomarker enzymes when compared along with standard drug Liv 52. The hydroalcoholic extract showed better activity compared to ethyl acetate extracts. The percentage protection on liver biomarker enzymes levels of hydro-alcoholic extract at 100, 200, and 400 mg/kg on AST, ALT, ALP, T.ptn, and T.Bil was found to be 62.72%, 60.06%, 61.77%, 63.96% and 58.58% respectively. Conclusion: The results of recent study support traditional medicinal use of Acampe praemorsa and provides the information about its’ hepatoprotective nature. The hepatoprotective activity of A. praemorsa was definitely due to presence of different phytochemical compounds in it as it was compared with Liv 52 which was also an herbal drug.

scholarly journals Evaluation of in vivo Hepatoprotective activity of Mimosa rubicaulis (LAM.) against CCL4 Induced Liver Toxicity

2021 ◽  
Vol 69 (1) ◽  
Author(s):  
Ashpak M. Tamboli ◽  
Kiran A. Wadkar
Keyword(s):  
Antioxidant Enzymes ◽  
Wistar Rats ◽  
Liver Toxicity ◽  
Chemical Constituents ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Treated Animal ◽  
Ccl4 Administration ◽  
Female Wistar Rats

The current study was conducted to assess the hepatoprotective activity of different extracts of M. rubicaulis (Lam.) at (200 and 400 mg/kg) doses b.w. against CCl4 induced liver intoxication in Albino Wistar rats. Male or female Wistar rats about 150 and 200 gm body weight were selected for present study. The animals were divided into thirteen groups, six rats in each group. The extracts and Silymarin-treated animal groups significantly reduced the activities of various biochemical markers such as SGPT, SGOT, ALP, and TB which were elevated by carbon tetrachloride (CCl4) intoxication. The extracts of M. rubicaulis (Lam.) showed a dose dependent hepatoprotection activity. Among all the extracts, ethanolic extract produced maximum hepatoprotection (SGPT-83.15 %, SGOT76.82%, ALP-79.33%, TB-80.00%) at a 400mg/kg dose. After CCl4 administration, the levels of hepatic-antioxidant enzymes such as Glutathione (GSH) and Catalase (CAT) were reduced, whereas the level of hepatic lipid peroxidation (-LPO) increased. These hepatic antioxidant enzymes were also restored to normal levels by extracts and Silymarin treatment. The results of the present investigation indicate that all the extracts of M. rubicaulis (Lam.) possess hepatoprotective activity which may be due to the presence of various chemical constituents.

Hepatoprotective Potential of Elsholtzia densa Against Acute and Chronic Models of Liver Damage in Wistar Rats

Drug Research ◽  
2018 ◽  
Vol 68 (10) ◽  
pp. 567-575 ◽  
Author(s):  
Ovais Zargar ◽  
Rohina Bashir ◽  
Showkat Ganie ◽  
Akbar Masood ◽  
Mohammad Zargar ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Liver Toxicity ◽  
Methanolic Extract ◽  
Serum Levels ◽  
Hepatoprotective Activity ◽  
Control Group ◽  
Dependent Manner ◽  
Protein Levels ◽  
Chronic Models ◽  
Dose Dependent

AbstractThe aim of the present study was to evaluate the hepatoprotective activity of methanolic extract of Elsholtzia densa against experimentally induced acute (CCl4) and chronic (paracetamol) liver injury in albino wistar rats. Activity was measured by monitoring the serum levels of ALT, ALP AST and LDH, total protein levels, bilirubin and albumin. The results of the CCl4 and paracetamol-induced liver toxicity experiments showed that the rats treated with the methanolic extract of Elsholtzia densa exhibited a significant decrease in biochemical parameters as well as the proteins, which were all elevated in the CCl4 and paracetamol group. The extract at a concentration of 300 mg/kg body wt. showed a significant decline (P≤0.05) in the levels of AST, ALT, ALP and LDH to 69.50±2.23IU/L, 60.01±2.25IU/L,46.20±2.24 IU/L and 150.21±5.68IU/L in CCl4 injected animals and 51.12±2.20 IU/L,49.15±3.25 IU/L, 44.12±2.56 IU/L and 125.15±4.45 IU/L in paracetamol-treated animals when compared to the control group. The activities of tissue antioxidants GSH, GPx, GR, GST and CAT was significantly (P≤0.05) restored in dose dependent manner in animals treated with extracts as with acute and chronic hepatotoxic models. The current study confirmed the hepatoprotective effect of methanolic extract of Elsholtzia densa against the model hepatotoxicant CCl4 and paracetamol.

scholarly journals Hepatoprotective activity of Cuscuta reflexa aqueous and alcoholic extracts against CCl4 induced toxicity in rats

2020 ◽  
pp. 463-466
Author(s):  
Rakesh RANJAN ◽  
Manoj KUMAR ◽  
Amar KUMAR ◽  
Manoranjan Prasad SINHA
Keyword(s):  
Low Dose ◽  
Total Bilirubin ◽  
Hepatoprotective Activity ◽  
Albino Rats ◽  
High Dose ◽  
Alcoholic Extract ◽  
Cuscuta Reflexa ◽  
Ccl4 Administration ◽  
Altered Liver ◽  
The Impact

Introduction In the present study the impact of aqueous and alcoholic extracts of Cuscuta reflexa were investigated on rats intoxicated with carbon tetrachloride (CCl4). CCl4 is known to intoxicate the liver of rats which can be easily be observed by examining the total protein, total bilirubin, alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine transaminase ALT). Cuscuta reflexa have been used in traditional medicine culture from time immemorial. In this study both the aqueous and alcoholic extracts of Cuscuta reflexa were found to have curative impact on liver profile of CCl4 intoxicated rats. Materials and Methods. The stem of Cuscuta reflexa were collected from host bougainvillea, dried in shade and were subjected to alcoholic (ethanolic), and aqueous extraction. Albino rats were intoxicated with CCl4 to induce hepatotoxicity. The CCl4 intoxicated rats were treated with low dose and high dose of both the extracts to assess the hepatoprotective impact on intoxicated rats. The results clearly revealed that the CCl4 administration altered liver profile. The altered liver profile parameters recovered to normal after administration of aqueous and alcoholic extracts. Results and Discussion. Administration of CCl4 induced hepatotoxicity in albino rats, which was evident with the results of serum analysis. Post CCl4 administration the liver profile parameters were altered. The CCl4 intoxicated rats were then treated with aqueous and alcoholic extracts of Cuscuta reflexa. Post treatment with the extracts the liver profile parameters recovered to normal. Conclusion: Present study reveals that aqueous and alcoholic extracts of Cuscuta reflexa were found to have curative impact on liver profile of CCl4 intoxicated rats. Keywords: Custuca reflexa, CCl4, hepatoprotective, liver profile, alcoholic extract, aqueous extract,

scholarly journals In-vivo Hepatoprotective and Antioxidant Activities of Sphaeranthus amaranthoides Burm.f. Against Anti-Tubercular Drugs Induced Hepatotoxicity in Rats

2021 ◽  
pp. 15-23
Author(s):  
M. R. Vinayakamurthi ◽  
J. Anbu Jeba Sunilson ◽  
A. V. Anita Gnana Kumari ◽  
U. Aathilakshmi
Keyword(s):  
Antioxidant Activity ◽  
Liver Toxicity ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Liver Homogenate ◽  
Hepatoprotective Activity ◽  
Thiobarbituric Acid Reactive Substance ◽  
Enzymatic Antioxidants ◽  
Drug Induced

Aim: To evaluate the hepatoprotective and antioxidant activity of Sphaeranthus amaranthoides Burm.f. against isoniazid (INH) and rifampicin (RIF) induced hepatotoxicity in rats. Study Design: Experimental study. Place and Duration: Research lab, Department of Siddha Medicine, Tamil University, Thanjavur, India, between March 2018 and November 2019. Methodology: Liver toxicity was induced by antitubercular drugs (Isoniazid; INH+Rifampicin; RIF) at a dose level of 50+100 mg/kg each, p.o for 15 days. Petroleum ether, Chloroform, Methanol, Aqueous extracts of Sphaeranthus amaranthoides Burm.f. (S. amaranthoides) (200 and 400 mg/kg bt.wt.) were administered orally once daily for 15 days. The hepatoprotective activity was assessed using various biochemical parameters SGOT, SGPT, ALP, bilirubin, total protein, albumin, total cholesterol, total bilirubin, direct bilirubin and LDH. The antioxidant activities such as the enzymatic activity of superoxide dismutase (SOD), and catalase (CAT), and the level of lipidperoxidation as thiobarbituric acid reactive substance (TBA-RS) were measured in liver homogenates and histological examinations were carried out to assess hepatoprotective activity. For Statistical analysis, the values were subjected to one way analysis of variance (ANOVA) followed by Tukey multiple compare test. Results were considered statistically significant when P<0.05. Results: The treatment with methanolic extract (400 mg/kg bt.wt.) of S. amaranthoides significantly prevented drug-induced increase in serum levels of liver enzymes (P<0.001). The antioxidant activity of a dose of 400 mg/kg of S. amaranthoides significantly prevented the decreases in the activity of enzymatic antioxidants (CAT & SOD) (P<0.01 and P<0.001) and inhibited the elevation of lipid peroxidation (TBA-RS) in the liver homogenate. Histopathology of liver tissue showed that S. amaranthoides attenuated the hepatocellular necrosis, regeneration and repair of cells toward normal. Conclusion: The methanolic extract of S. amaranthoides showed significant hepatoprotectivity and antioxidant activity against INH + RIF Anti TB drugs.

Antioxidant and hepatoprotective activity of Tragopogon porrifolius methanolic extract

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
M Mroueh ◽  
C Daher ◽  
M El Sibai ◽  
C Tenkerian
Keyword(s):  
Methanolic Extract ◽  
Hepatoprotective Activity

Hepatoprotective activity of the wild carrot chloroform-based fraction against CCl4-induced liver toxicity in mice

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
W Shebaby ◽  
M El-Sibai ◽  
M Mroueh ◽  
K Bodman-Smith ◽  
R Taleb ◽  
...  
Keyword(s):  
Liver Toxicity ◽  
Hepatoprotective Activity ◽  
Wild Carrot

Liver Toxicity in Rats Fed A Mixture of Pcb Congeners and 2,3,7,8-Tcdd

1997 ◽  
Vol 3 (S2) ◽  
pp. 51-52
Author(s):  
B.J. Cornell ◽  
A. Singh ◽  
I. Chu
Keyword(s):  
Aromatic Compounds ◽  
Liver Toxicity ◽  
Corn Oil ◽  
Morphological Changes ◽  
Test Substance ◽  
Sprague Dawley ◽  
Morphological Alterations ◽  
Pcb Congeners ◽  
Transmission Electron ◽  
Sprague Dawley Rats

Polyhalogenated aromatic compounds such as polychlorinated biphenyls (PCBs) and polychlorinated dibenzo-p-dioxins (PCDDs) continue to be environmental contaminants because of their bioaccumulation in the food chain and high resistance to biodegradation. The current study was undertaken to determine if a mixture of PCB congeners (WHO-IPCS) were interactive with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in producing morphological changes in the rat liver. Both compounds are known to produce a broad range of biochemical and morphological alterations including enzyme induction.Groups (N=5) of female Sprague-Dawley rats were administered TCDD (0, 2.5, 25, 250, 1000 ng/kg bw/day) or PCB (0, 2, 20 μg/kg bw/day) alone, or in combination with each concentration of both compounds. Incorrect concentrations were published in a previous abstract. The test substance was mixed with corn oil and given by gavage at 2 ml/kg daily for 28 days. At the end of the experiment, the rats were killed and liver samples were prepared for transmission electron microscopy.

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