Effect of germination on in vitro and in vivo activity of Lupinus albus L. and Lupinus angustifolius L. seed extract

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
C Danciu ◽  
E Alexa ◽  
I Zupko ◽  
S Avram ◽  
I Zinuca Pavel ◽  
...  
Keyword(s):  
Lupinus Albus ◽  
Seed Extract ◽  
Lupinus Angustifolius ◽  
Lupinus Albus L

scholarly journals Germinated and Ungerminated Seeds Extract from TwoLupinusSpecies: Biological Compounds Characterization and In Vitro and In Vivo Evaluations

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Bogdan Andor ◽  
Corina Danciu ◽  
Ersilia Alexa ◽  
Istvan Zupko ◽  
Elena Hogea ◽  
...  
Keyword(s):  
Cinnamic Acid ◽  
Therapeutic Potential ◽  
Lupinus Albus ◽  
Biological Evaluation ◽  
Lupinus Angustifolius ◽  
Cinnamic Acid Derivatives ◽  
Natural Agents ◽  
Germinated Seeds

In recent years, nutraceuticals attracted a great amount of attention in the biomedical research due to their significant contribution as natural agents for prevention of various health issues. Ethanolic extracts from the ungerminated and germinated seeds ofLupinus albusL. andLupinus angustifoliusL. were analyzed for the content in isoflavones (genistein) and cinnamic acid derivatives. Additionally, the extracts were evaluated for antimicrobial, antiproliferative, and anti-inflammatory properties, using in vitro and in vivo tests. Germination proved to be a method of choice in increasing the amount of genistein and cinnamic acid derivatives in bothLupinus albusL. andLupinus angustifolius L.seeds. Biological evaluation of all vegetal extracts revealed a weak therapeutic potential for both ungerminated and germinated seeds.

In vitro and in vivo bio-stimulatory properties of a Lupinus albus L. seed suspension

2011 ◽  
Vol 62 (3) ◽  
pp. 189 ◽  
Author(s):  
Elmarie van der Watt ◽  
Johan C. Pretorius
Keyword(s):  
Root Growth ◽  
Respiration Rate ◽  
Seedling Growth ◽  
Lupinus Albus ◽  
Positive Control ◽  
Seedling Root ◽  
Stimulatory Activity ◽  
Lupinus Albus L

During an extended screening program of plant extracts for bio-stimulatory activity, a seed suspension (SS) of Lupinus albus L. cv. Betsuhana White was identified as most promising. By testing a concentration range of SS using three in vitro bio-assays [i.e. respiration rate of monoculture yeast (Saccharomyces cerevisiae) cells, germination rate of seeds from selected crops, and subsequent seedling growth], 5 mg/L was identified as the optimal concentration in terms of in vitro bio-stimulatory activity. A commercially available bio-stimulant, ComCat®, was used as a positive control, while a glucose solution for respiration tests and distilled water for germination tests served as negative controls. Compared with the controls, significant (P < 0.05) increases in the respiration rate of monoculture yeast cells and seedling root growth in the majority of test crops after treatment with SS confirmed its in vitro bio-stimulatory potential. Seedling root growth compared favourably with results obtained with the commercial bio-stimulant. Subsequently, the in vivo yield response of selected crops to foliar treatment with SS was investigated under field conditions using ComCat® as a positive control. All trials were laid out in a complete randomised block design. Foliar applications of SS at 5 mg/L under a drip irrigation system contributed to significant (P < 0.05) yield increases in lettuce (by 20.0 t/ha) and carrots (by 22.2 t/ha), which compared favourably with the positive control, but not in beetroot and cabbage. Beetroot yield was enhanced whereas cabbage yield was decreased markedly, but not significantly, by the SS treatment, compared with the negative control. The results confirmed both the in vitro and in vivo bio-stimulatory potential of a seed suspension of L. albus cv. Betsuhana White in terms of its ability to manipulate early seedling growth and enhance the yield of selected vegetable crops.

scholarly journals Isolamento da globulina majoritária, digestibilidade in vivo e in vitro das proteínas do tremoço-doce (Lupinus albus L.), var. Multolupa

2006 ◽  
Vol 26 (4) ◽  
pp. 832-840 ◽  
Author(s):  
Valdir Augusto Neves ◽  
Sinézio Inácio Silva Jr. ◽  
Maraiza Aparecida da Silva
Keyword(s):  
Lupinus Albus ◽  
Lupinus Albus L

Grape Seed Extract Complex (FFM-GS) Inhibits Postmenopausal Symptoms: In Vitro and In Vivo S

2019 ◽  
Vol 48 (3) ◽  
pp. 297-305
Author(s):  
Minhee Lee ◽  
Da-Eun Nam ◽  
Soo-Jeung Park ◽  
Dakyung Kim ◽  
Jeong-Moon Yun ◽  
...  
Keyword(s):  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Postmenopausal Symptoms

Aqueous extracts of avocado pear (Persea americana Mill.) leaves and seeds exhibit anti-cholinesterases and antioxidant activities in vitro

2016 ◽  
Vol 27 (2) ◽  
Author(s):  
Ganiyu Oboh ◽  
Veronica O. Odubanjo ◽  
Fatai Bello ◽  
Ayokunle O. Ademosun ◽  
Sunday I. Oyeleye ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Inhibitory Effect ◽  
Seed Extract ◽  
Persea Americana ◽  
Dependent Manner ◽  
Natural Treatment

AbstractAvocado pear (The inhibitory effects of extracts on AChE and BChE activities and antioxidant potentials (inhibition of FeThe extracts inhibited AChE and BChE activities and prooxidant-induced TBARS production in a dose-dependent manner, with the seed extract having the highest inhibitory effect and the leaf extract exhibiting higher phenolic content and radical scavenging abilities, but lower Fe chelation ability compared with that of the seed. The phytochemical screening revealed the presence of saponins, alkaloids, and terpenoids in both extracts, whereas the total alkaloid profile was higher in the seed extract than in the leaf extract, as revealed by GC-FID.The anti-cholinesterase and antioxidant activities of avocado leaf and seed could be linked to their phytoconstituents and might be the possible mechanisms underlying their use as a cheap and natural treatment/management of AD. However, these extracts should be further investigated in vivo.

Phytochemical Screening and Anti-Diabetic Efficacy of O. Sanctum Seed Extract on Streptozotocin Induced Diabetic Rats

2017 ◽  
Vol 54 (3) ◽  
pp. 336
Author(s):  
Kavitha K. ◽  
Ponne S.
Keyword(s):  
Body Weight ◽  
Digestive Enzymes ◽  
Fasting Blood Glucose ◽  
Inhibitory Effect ◽  
Diabetic Rats ◽  
Seed Extract ◽  
Phytochemical Screening ◽  
Weight Changes

The present study was designed to assess the in vitro and in vivo anti-diabetic efficacy of <em>O. sanctum</em> seed and its phytochemical screening. In vitro inhibitory effect on carbohydrate digestive enzymes like α-amylase and α-glucosidase and in vivo parameters such as fasting blood glucose and body weight changes were studied, a potent inhibitory effect was observed on activities of digestive enzymes and a marked decrease in the glucose level in the <em>O. sanctum</em> seed extract treated streptozotocin induced diabetic rats was noted. Further a marked reduction in body weight was also observed.

Missing pieces in protein deposition and mobilization inside legume seed storage vacuoles: calcium and magnesium ions

2012 ◽  
Vol 22 (4) ◽  
pp. 249-258 ◽  
Author(s):  
Cláudia N. Santos ◽  
Marta M. Alves ◽  
Isabel T. Bento ◽  
Ricardo B. Ferreira
Keyword(s):  
Alkaline Earth ◽  
Storage Proteins ◽  
Lupinus Albus ◽  
Physiological Role ◽  
Experimental Conditions ◽  
Protein Storage Vacuoles ◽  
Aggregation And Disaggregation ◽  
Alkaline Earth Cations

AbstractDuring the maturation of dicotyledonous seeds, organic carbon, nitrogen and sulphur are stored in protein storage vacuoles (PSVs) as storage globulins. Several studies point to the coexistence of storage proteins with proteases responsible for their degradation inside PSVs. Different mechanisms have been proposed to explain why there is no proteolysis during this period. Protein aggregation to form large supramolecular structures resistant to proteolytic attack could be the reason. However, during germination, and particularly following its completion, the globulin aggregates must undergo disintegration to allow protease attack for protein reserve mobilization. Based on the well-described concentration-dependent ability of Ca2+ and Mg2+ to promote in vitro aggregation and disaggregation of globulins, we explored a possible role for these alkaline earth cations in globulin packaging and mobilization. Ca2+ and Mg2+ measurements in purified PSVs [6.37 μmol and 43.9 μmol g− 1 dry weight (DW) of cotyledons, respectively] showed the presence of these two alkaline earth cations within this compartment. To our knowledge, this is the first time that Ca2+ and Mg2+ have been quantified in purified PSVs from Lupinus albus seeds. Considering the importance of these two alkaline earth cations inside PSVs, which represent 14.6% and 60.7% of the total seed Mg2+and Ca2+, respectively, globulin aggregation and disaggregation profiles were assayed using experimental conditions closer to those that are physiologically present (proportion of Ca2+ and Mg2+, and acidic pH). Based on: (1) the high in vivo abundance of Ca2+ and Mg2+ inside PSVs; and (2) globulin aggregation and disaggregation profiles, together with structural and physiological evidence already reported in the literature, an important physiological role for Ca2+ and Mg2+ in globulin packaging and mobilization inside PSVs is suggested.

scholarly journals Grape Seed Extract Inhibits In vitro and In vivo Growth of Human Colorectal Carcinoma Cells

2006 ◽  
Vol 12 (20) ◽  
pp. 6194-6202 ◽  
Author(s):  
Manjinder Kaur ◽  
Rana P. Singh ◽  
Mallikarjuna Gu ◽  
Rajesh Agarwal ◽  
Chapla Agarwal
Keyword(s):  
Colorectal Carcinoma ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Carcinoma Cells ◽  
Human Colorectal Carcinoma ◽  
In Vivo Growth
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