Coagulation Changes with intravenous Administration of Fat after Blocking the RES

1966 ◽  
Vol 15 (03/04) ◽  
pp. 381-389
Author(s):  
B Kommerell ◽  
P Barth ◽  
Th Pfleiderer
Keyword(s):  
Platelet Count ◽  
Intravenous Administration ◽  
Death Rate ◽  
Coagulation System ◽  
India Ink ◽  
High Death Rate ◽  
High Death ◽  
Group A ◽  
Group B

SummaryRabbits were treated in 3 groups : Group A received 20 ml/kg Lipofundin, group B received 150 mg/kg india ink and 20 ml/kg Lipofundin, group C received 150 mg/kg india ink and 20 ml/kg isotonic glucose. Group A and B showed a decrease of activity of the factors II, V, VII, serumcomplex and a decrease of the platelet count. The changes were more pronounced in group B and 6 of 8 animals of this group died after the infusion. In group A only 1 animal died. All controll animals (group C) survived. There were no significant changes in the coagulation system in group C. The contact factors showed an activation in group B, little activation in group A and inconsistent activation in group C. In vitro india ink was able to produce a moderate activation of the contact system. The high death rate after administration of india ink and fat is thought to be due to a consumptive coagulopathy.

scholarly journals Antiparasitic Activity of Single Solanum ferox Extract and Concoction with Zingiber zerumbet and Boesenbergia pandurata Extracts to Control Argulus sp. on Goldfish (Cyprinus carpio)

2021 ◽  
Author(s):  
Esti Handayani Hardi ◽  
Rudy Agung Nugroho ◽  
Irawan wijaya kusuma ◽  
gina Saptiani ◽  
agustina agustina ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Death Rate ◽  
In Vitro Testing ◽  
Testing Time ◽  
Antiparasitic Activity ◽  
Zingiber Zerumbet ◽  
High Death Rate ◽  
High Death

Argulus is an ectoparasite that frequently infects goldfish, with severe effects and a high death rate. The use of plant extracts has been widely reported to have antiparasitic activities, including one of the solasodine compounds in sour eggplant extracts. This research evaluated the antiparasitic activities of Compositions A (Solanum ferox and Zingiber zerumbet extract at 400 and 200 ppm, respectively), B (SFE and Boesenbergia pandurata at 900 ppm), and C (single Solanum ferox at 400 ppm). The research stages comprised 1) isolation and analysis of solasodine content in sour eggplant extracts and its extract composition, 2) in vitro antiparasitic activities on Argulus sp. at doses of 50 and 100 ppm with a testing time of 60–240 minutes, and 3) in vivo antiparasitic activities on Argulus sp. on goldfish at a dose of 100 ppm and observation for 2–12 hours of medication. The results showed that sour eggplant extracts had a solasodine concentration of 7.151 mg/L, whereas the derivative compositions A, B, and C were 656, 485, and 295 ppm, respectively. The in vitro testing demonstrated that composition A was effective for killing approximately 80–100% of Argulus, whereas compositions B and C killed 80–90% and 60–70%, respectively. Viewed from the effectiveness for killing parasites, the result was excellent (above 50%). The in vivo medication test was continued using three extract compositions at a dose of 100 ppm. Compositions A, B, and C were found to be capable of releasing 81.33, 75.67, and 71.00 arguli, respectively, per fish. We concluded that the single SFE extract and a concoction with BPE and ZZE had reasonable antiparasitic activity, whereas the concoction of SFE and ZZE killed more Argulus parasites at a higher rate.

Factor II Related Antigen and Antithrombin III Levels as Indicators of Liver Failure in Consumption Coagulopathy

1982 ◽  
Vol 47 (03) ◽  
pp. 218-220 ◽  
Author(s):  
P Sié ◽  
E Letrenne ◽  
C Caranobe ◽  
M Genestal ◽  
B Cathala ◽  
...  
Keyword(s):  
Liver Failure ◽  
Antithrombin Iii ◽  
Coagulation Factors ◽  
Consumption Coagulopathy ◽  
Blood Coagulation Factors ◽  
Factor Ii ◽  
At Iii ◽  
Group A ◽  
Group B

SummaryIn order to detect impaired synthesis of blood coagulation factors associated to consumption coagulopathy, a simultaneous evaluation of factor II-related antigen (II rAg) and of antithrombin III (AT III) was carried out in 16 patients affected with severe defibrination. An in vitro preliminary study on plasma and serum demonstrated that the levels of II rAg and of AT III, assessed by the Laurell technique with Behring antisera, were not reduced by the coagulation process. The patients were, a posteriori, classified into two groups according to the absence (group A) or the presence (group B) of factors predisposing to liver failure such as metastasis, cirrhosis, and prolonged shock. II rAg and AT III levels are significantly correlated; they are in the normal range in group A but reduced in group B. Thus II rAg or AT III level determinations are useful markers in the detection of liver failure associated to the consumption phenomenon. These results also suggest that part of the decreased AT III levels reported in severe cases of disseminated intravascular coagulation may be the consequence of an associated liver failure.

scholarly journals The Impact of Left Atrial Size in Catheter Ablation of Atrial Fibrillation Using Remote Magnetic Navigation

2018 ◽  
Vol 2018 ◽  
pp. 1-8
Author(s):  
Xiao-yu Liu ◽  
Hai-feng Shi ◽  
Jie Zheng ◽  
Ku-lin Li ◽  
Xiao-xi Zhao ◽  
...  
Keyword(s):  
Atrial Fibrillation ◽  
Catheter Ablation ◽  
Left Atrial ◽  
Magnetic Navigation ◽  
Remote Magnetic Navigation ◽  
X Ray ◽  
Group A ◽  
Group B ◽  
The Impact

Objective. The objective of this study was to investigate the impact of left atrial (LA) size for the ablation of atrial fibrillation (AF) using remote magnetic navigation (RMN). Methods. A total of 165 patients with AF who underwent catheter ablation using RMN were included. The patients were divided into two groups based on LA diameter. Eighty-three patients had small LA (diameter <40 mm; Group A), and 82 patients had a large LA (diameter ≥40 mm; Group B). Results. During mapping and ablation, X-ray time (37.0 (99.0) s vs. 12 (30.1) s, P<0.001) and X-ray dose (1.4 (2.7) gy·cm2 vs. 0.7 (2.1) gy·cm2, P=0.013) were significantly higher in Group A. No serious complications occurred in any of the patients. There was no statistical difference in the rate of first anatomical attempt of pulmonary vein isolation between the two groups (71.1% vs. 57.3%, P=0.065). However, compared with Group B, the rate of sinus rhythm was higher (77.1% vs. 58.5%, P<0.001) during the follow-up period. More patients in Group A required a sheath adjustment (47/83 vs. 21/82, P<0.001), presumably due to less magnets positioned outside of the sheath. In vitro experiments with the RMN catheter demonstrated that only one magnet exposed created the sheath affects which influenced the flexibility of the catheter. Conclusions. AF ablation using RMN is safe and effective in both small and large LA patients. Patients with small LA may pose a greater difficulty when using RMN which may be attributed to the fewer magnets beyond the sheath. As a result, the exposure of radiation was increased. This study found that having at least two magnets of the catheter positioned outside of the sheath can ensure an appropriate flexibility of the catheter.

Salivary oestradiol and progesterone after in vitro fertilization and embryo transfer using different luteal support regimens

1990 ◽  
Vol 2 (4) ◽  
pp. 351 ◽  
Author(s):  
YF Wong ◽  
EP Loong ◽  
KR Mao ◽  
PP Tam ◽  
NS Panesar ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Transfer ◽  
Luteal Phase ◽  
Biologically Active ◽  
Luteal Support ◽  
Vitro Fertilization ◽  
Group A ◽  
Group B ◽  
Ivf Et

Salivary oestradiol (E2) and progesterone (P) levels have been shown to reflect the biologically active fractions in the serum. The luteal-phase status of stimulated cycles was investigated after in vitro fertilization and embryo transfer (IVF-ET). Thirty patients were randomly allocated to one of three luteal therapy groups: group A had no support, group B had intramuscular P and group C had intramuscular P and human chorionic gonadotrophin (hCG). One pregnancy was achieved in group A, two in group B and three in group C. Significant correlations between salivary and serum levels of E2 and of P in matched samples during luteal phase were found. Salivary E2 levels from luteal day 8 through day 14 and P levels from day 3 through day 14 were significantly higher in the pregnant than in the nonpregnant cycles. Among the nonpregnant cycles, salivary E2 and P levels were significantly higher in group C than in group A or B. These findings suggest that, in stimulated cycles for IVF-ET, determination of salivary E2 and P levels may be used as reliable alternatives to serum concentrations for assessing the luteal phase. Also, the additional hCG has an enhanced luteotrophic effect, as reflected by the higher salivary E2 and P levels, which may lead to a better pregnancy rate.

Effects of Mechanical Stimulation on MMP-2 and TIMP-2 Expression of Scleral Fibroblasts after Posterior Sclera Reinforcement

2014 ◽  
Vol 490-491 ◽  
pp. 867-871
Author(s):  
Guo Hui Wang ◽  
Wei Yi Chen
Keyword(s):  
Mechanical Stimulation ◽  
Sine Wave ◽  
Posterior Pole ◽  
Cyclic Stretch ◽  
Elisa Method ◽  
Band Group ◽  
Group A ◽  
Operation Group ◽  
Group B

To understand the effect of mechanical stimulation on posterior sclera reinforcement (PSR), the rabbit scleral fibroblasts after PSR were subjected to stretch in vitro and MMP-2 and TIMP-2 expression of scleral fibroblasts were evaluated. Three-week-old rabbits were monocularly performed by eyelid suturation randomly to prepare experimental myopia eye. After 60 days, the experimental myopia eyes were treated by PSR. After 6 months, the posterior pole scleral fibroblasts (normal sclera - group A, sclera after operation - group B and fusion region of sclera and reinforcing band group C) were isolated and cultured in vitro. The cells were subjected to cyclic stretch regimens (sine wave, 3% and 6% elongation amplitude, 0.1Hz, 48h duration) by FX-4000 Tension System. The MMP-2 and TIMP-2 expression of scleral fibroblasts were evaluated by ELISA method. The results show that after cyclic stretch to the scleral fibroblasts of the normal sclera and the sclera after operation, the MMP-2 expression was significantly reduced and the TIMP-2 expression was significantly increased, the MMP-2 and TIMP-2 expression of the scleral fibroblasts of the fusion region after operation was no changed. It was indicated that the mechanical stimulation could regulate the MMP-2 and TIMP-2 expression of scleral fibroblasts and play an important role in the process of treating high myopia with PSR surgery.

scholarly journals Photopolymerizable Injectable Cartilage Mimetic Hydrogel for the Treatment of Focal Chondral Lesions: A Proof of Concept Study in a Rabbit Animal Model

2018 ◽  
Vol 47 (1) ◽  
pp. 212-221 ◽  
Author(s):  
Cecilia Pascual-Garrido ◽  
Elizabeth A. Aisenbrey ◽  
Francisco Rodriguez-Fontan ◽  
Karin A. Payne ◽  
Stephanie J. Bryant ◽  
...  
Keyword(s):  
Animal Model ◽  
Chondrogenic Differentiation ◽  
Osteochondral Lesions ◽  
Chondral Defect ◽  
Group A ◽  
Group B ◽  
Safranin O

Background: In this study, we investigate the in vitro and in vivo chondrogenic capacity of a novel photopolymerizable cartilage mimetic hydrogel, enhanced with extracellular matrix analogs, for cartilage regeneration. Purpose: To (1) determine whether mesenchymal stem cells (MSCs) embedded in a novel cartilage mimetic hydrogel support in vitro chondrogenesis, (2) demonstrate that the proposed hydrogel can be delivered in situ in a critical chondral defect in a rabbit model, and (3) determine whether the hydrogel with or without MSCs supports in vivo chondrogenesis in a critical chondral defect. Study Design: Controlled laboratory study. Methods: Rabbit bone marrow–derived MSCs were isolated, expanded, encapsulated in the hydrogel, and cultured in chondrogenic differentiation medium for 9 weeks. Compressive modulus was evaluated at day 1 and at weeks 3, 6, and 9. Chondrogenic differentiation was investigated via quantitative polymerase reaction, safranin-O staining, and immunofluorescence. In vivo, a 3 mm–wide × 2-mm-deep chondral defect was created bilaterally on the knee trochlea of 10 rabbits. Each animal had 1 defect randomly assigned to be treated with hydrogel with or without MSCs, and the contralateral knee was left untreated. Hence, each rabbit served as its own matched control. Three groups were established: group A, hydrogel (n = 5); group B, hydrogel with MSCs (n = 5); and group C, control (n = 10). Repair tissue was evaluated at 6 months after intervention. Results: In vitro, chondrogenesis and the degradable behavior of the hydrogel by MSCs were confirmed. In vivo, the hydrogel could be delivered intraoperatively in a sterile manner. Overall, the hydrogel group had the highest scores on the modified O’Driscoll scoring system (group A, 17.4 ± 4.7; group B, 13 ± 3; group C, 16.7 ± 2.9) ( P = .11) and showed higher safranin-O staining (group A, 49.4% ± 20%; group B, 25.8% ± 16.4%; group C, 36.9% ± 25.2%) ( P = .27), although significance was not detected for either parameter. Conclusion: This study provides the first evidence of the ability to photopolymerize this novel hydrogel in situ and assess its ability to provide chondrogenic cues for cartilage repair in a small animal model. In vitro chondrogenesis was evident when MSCs were encapsulated in the hydrogel. Clinical Relevance: Cartilage mimetic hydrogel may offer a tissue engineering approach for the treatment of osteochondral lesions.

scholarly journals In vitro Comparison of Apically Extruded Debris during Root Canal Retreatment with Rotary and Reciprocating Systems

2019 ◽  
Vol 13 (1) ◽  
pp. 488-492
Author(s):  
María J. Burbano Balseca ◽  
Silvana B. Terán Ayala ◽  
Fábio D. da Costa Aznar ◽  
Adriana R. de Freitas-Aznar ◽  
Guillermo M. Aguirre Balseca ◽  
...  
Keyword(s):  
Similar Amount ◽  
Hybrid Technique ◽  
Significance Level ◽  
Sealing Material ◽  
Apical Extrusion ◽  
Root Canal Retreatment ◽  
Group A ◽  
Group B ◽  
Reciprocating Systems

Introduction: The extrusion of apical detritus during the retracting procedure is very important, as, besides eliminating materials of radicular obturation, aggressive agents and products such as the remains of dentin taken out, and microorganisms are generally located in tissue around the roots, producing undesired effects, such as inflammation, acute pain, post-operatory pain and delays in the periapical treatment. Objective: To determine the amount of apically extruded detritus using rotary and reciprocating retreatment systems. Methodology: An experimental comparative study as conducted in which 40 single-rooted human first premolars were analyzed. The instruments used were ProTaper Universal files up to F3 and sodium hypochlorite, and the sealing was made through the hybrid technique Tager. They were then kept at 37ºC and 100% of humidity for 15 days so that the sealing material would seal. For the retracting procedure, experimental tubes previously weighted were used. The sample was divided randomly into two groups of 20 pieces. Group A: ProTaper R, and group B: Reciproc. Then, the irrigating material (distilled water) was evaporated from the tubes for 12 hours in a stove at 105ºC, and the experimental tubes were weighted in an analytical precision weighing scale 10-5g. The data was analyzed through the T Student with a significance level of 5%. Results: Results indicated that groups A and B, retreatment with rotational ProTaper R and reciprocating retreatment system (Reciproc) showed a similar difference of initial and final weights, indicating similar apical extrusion of detritus. Conclusion: Both systems extruded a similar amount of detritus during the retreatment procedures.

scholarly journals Frictional Characteristics of the Newer Low-friction Elastomeric Ligatures

2016 ◽  
Vol 6 (1) ◽  
pp. 19-23
Author(s):  
Amol Mhatre ◽  
VK Ravindranath ◽  
Sachin Doshi ◽  
Girish Karandikar ◽  
PS Vivek
Keyword(s):  
Testing Machine ◽  
Frictional Resistance ◽  
Minimal Amount ◽  
New Delhi ◽  
Low Friction ◽  
Significant Difference ◽  
Group A ◽  
Group B ◽  
New Generation

ABSTRACT Aim The aim of this in vitro study was to investigate the efficiency of the new generation of elastomeric ligatures with innovative designs (SlideTM and AlastiKTM Easy-to-Tie) in reducing frictional resistance (FR) during sliding mechanics as compared with conventional ligatures. Materials and Methods Sixty ligature samples divided into four groups were used for the study. Group A: QuiK-StiK™ (3M Unitek, Monrovia, CA, USA), Group B: AlastiK™ Easy-to-Tie (3M Unitek, Monrovia, CA, USA), Group C: Slide™ (Leone, Firenze, Italy), and Group D: SS ligatures 0.010” (Libral Traders, New Delhi, India). Universal Testing Machine, Instron was used for measuring FR at the bracket-wire interface. Results There was statistically significant difference in FR among all the four groups of ligatures tested (p < 0.001). Slide ligatures produced the least amount of FR followed by SS ligatures, Easy-to-Tie, and QuiK-StiK in the increasing order of the FR values registered. Conclusion SlideTM ligatures may represent a valid alternative to passive self-ligating brackets when minimal amount of friction is desired. Angulation introduced into the elastomeric ligatures reduces the friction in comparison to conventional elastomeric ligatures. How to cite this article Vivek PS, Ravindranath VK, Karandikar G, Doshi S, Mhatre A, Sonawane M. Frictional Characteristics of the Newer Low-friction Elastomeric Ligatures. J Contemp Dent 2016;6(1):19-23.

scholarly journals Causes of spontaneous death and euthanasia in dogs: A background study in Bahia, Brazil

2021 ◽  
Vol 66 (No. 4) ◽  
pp. 156-166
Author(s):  
JL Freitas ◽  
TS Andrade ◽  
ES Martins Filho ◽  
CS D’Soares ◽  
ACSN Souza ◽  
...  
Keyword(s):  
Death Rate ◽  
Northeast Brazil ◽  
Disease Category ◽  
Pathology Laboratory ◽  
High Death Rate ◽  
High Death ◽  
Veterinary Pathology ◽  
Diagnostic Capabilities ◽  
Health Programmes ◽  
Epidemiological Information

Understanding the causes of death in dogs enhances the diagnostic capabilities of veterinarians, as well as reduces animal mortality. Studies on this topic assist in the control and prophylaxis of epidemics and in structuring public health programmes. In total, 1 355 necropsy reports of dogs filed at the Veterinary Pathology Laboratory of the Federal University of Bahia, Northeast, Brazil (2005–2017) were analysed. The epidemiological information and anatomopathological diagnoses were obtained. The diagnosed diseases were classified into 10 categories. The frequency of the zoonoses, the overall age of the death (AD) of the dogs, and the AD of the dogs for each disease category were determined. Infectious and parasitic diseases were the most frequent primary causes of death in puppies (44.62%) and adult dogs (26.52%). In elderly dogs, most deaths occurred due to neoplasms (42.37%). Distemper (10.46%) was the most prevalent condition. A high frequency of zoonoses (6.12%) and a high death rate caused by diseases that could have been prevented (15.06%), such as distemper, parvovirus and canine visceral leishmaniasis, were reported. The AD in the population studied was eight years. The results confirmed the hypothesis of a high death rate in dogs in the state of Bahia, Northeast, Brazil, because of preventable infectious diseases.

scholarly journals DECLARATION OF EPIDEMIC PUBLIC HEALTH WITH NOVEL CORONAVIRUS

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Rafid A. Abdulkareem
Keyword(s):  
Public Health ◽  
Early Detection ◽  
Diagnostic Tests ◽  
Death Rate ◽  
Rna Viruses ◽  
Virus Disease ◽  
Respiratory Problems ◽  
High Death Rate ◽  
High Death ◽  
Novel Coronavirus

Coronaviruses (CoV) are broad enveloped RNA viruses in humans and animals are mostly correlated with enteric and respiratory problems. Within the last decades, incidents of high death rate triggered by the spread of CoV from animals to humans have occurred. Certain pathogen city of CoV is known species-dependent as with the extent of infection; Complications typically appear in closely associated human hosts. Epidemiologists assume that the SARS virus arose in bats (natural reservoirs hosts) that were transferred to persons in Wuhan, China. Presently, the available diagnostic tests are aimed at the traditional early detection of the causes of pneumonia, promote disease prevention efforts and collaborate with research laboratories that can conduct pan coronavirus detection or controlled sequencing. No vaccine is sufficient to protect toward coronaviruses. Also there is no clear treatment for corona virus disease. Patients take comprehensive medication in clinics and typically heal on their own after several times. A vaccine can take up to 45 months to develop. Let’s stay safe during this period of time. Keywords: Coronaviruses, Spreading, bats, diagnostic, vaccine

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