Toothbrush-Dentifrice Abrasion of Dental Sealants: An In Vitro Study

Abstract Objective This study sought to investigate the toothbrush-dentifrice abrasion of dental sealants. Materials and Methods Weight loss (∆W) and depth loss (∆D) were used as abrasion indicators. Sealant samples from nine products were soaked in dentifrice slurry and abraded by using a toothbrushing machine with a brushing force of 300 g. The mean percentages of ∆W and mean values of ∆D after 24,000 and 48,000 strokes of brushing were compared by using paired t-test. A comparison of these mean values among sealant products was performed by using one-way ANOVA and multiple comparison analysis (Scheffe's test). Results Abrasive wear was observed in all sealants. Teethmate F-1 (Kuraray Noritake, Tokyo, Japan)—a fluoride-releasing unfilled sealant—exhibited the maximum abrasive wear, with ∆W and ∆D values of 1.14% ± 0.37% and 12.84 ± 4.28 µm, respectively. Delton (Dentsply Sirona, Charlotte, North Carolina, United States), a light-cured unfilled sealant, showed the minimum abrasive wear, with ∆W and ∆D values of 0.41% ± 0.09% and 2.93 ± 1.23 µm, respectively. No statistical differences were observed among unfilled sealants except when compared with Teethmate F-1. Similarly, no differences were observed when comparing among filled sealants and flowable composite. Conclusion Abrasive wear occurred in all sealants after brushing with dentifrice. Almost all unfilled sealants showed less wear compared with both filled sealants and flowable composite. However, the low abrasive values of all sealants after brushing with dentifrice implied that there is no clinical significance to this finding.

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Surface Hardness of Hybrid Ionomer with Different Curing Time (In Vitro Study)

UI Proceedings on Health and Medicine ◽

10.7454/uiphm.v1i0.32 ◽

2017 ◽

Vol 1 ◽

Author(s):

Arfita Sipahutar ◽

Astrid Yudhit ◽

Rusfian Rusfian

Keyword(s):

Standard Deviation ◽

Surface Hardness ◽

In Vitro Study ◽

Hardness Test ◽

Curing Time ◽

Mean Values ◽

The Mean ◽

One Way Anova ◽

Group 1

Objective: The aim of this study was to evaluate the surface hardness of hybrid ionomer with different curing times (20, 30, 40, and 50 seconds).Methods: This was an experimental laboratory with posttest only group design. Twenty-four disk shape hybrid ionomer specimens with a diameter of 5 mm and 2 mm in thickness were prepared and polymerized with Quartz Tungsten Halogen (QTH) for 20, 30, 40, and 50 seconds as group 1, 2, 3, and 4 respectively. All samples were immersed in distilled water under 37°C temperature for 24 hours as initial treatment. Surface hardness test was done using Vickers Hardness Test with load 50 gf for 20 second. The Data was analyzed with one way ANOVA (p ≤ 0.05).Results: Using one way anova test (p ≤ 0.05) shows the mean and standard deviation of the group 1 is 25,900 ± 2,5659VHN; group 2 is 29,800 ± 1,1983 VHN; group 3 is 31,283 ± 0,5037 VHN; group 4 is 36, 183 ± 2,3430 VHN and the obtained value p = 0.000 (p ≤ 0.05).The mean values and standard deviation were 25.900 ± 2.5659 VHN; 29.800 ± 1.1983 VHN; 31.283 ±0.5037 VHN; 6.183 ± 2.3430 VHN for group 1, 2, 3, and 4 respectively. Statistic analyzed showed a significant different among groups with p value =0.000 (p < 0.05).Conclusion There are significant differences in surface hardness of hybrid ionomer with different curing time (20, 30,40, and 50 seconds). The longer curing time will increase the surface hardness of hybrid ionomer.

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Survey of Phenolic Acids, Flavonoids and In Vitro Antioxidant Potency Between Fig Peels and Pulps: Chemical and Chemometric Approach

Molecules ◽

10.3390/molecules26092574 ◽

2021 ◽

Vol 26 (9) ◽

pp. 2574

Author(s):

Lahcen Hssaini ◽

Francisca Hernandez ◽

Manuel Viuda-Martos ◽

Jamal Charafi ◽

Rachid Razouk ◽

...

Keyword(s):

Phenolic Acids ◽

Radical Scavenging ◽

Fruit Peel ◽

Mean Values ◽

Local Cultivar ◽

Ic50 Values ◽

The Mean ◽

Lipid Peroxidation Inhibition ◽

Almost All

In the present study, chromatic coordinates, phenolic acids, flavonoids and antioxidant capacity assessed by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonate (ABTS) and lipid peroxidation inhibition capacity (LPIC) essays and their relative IC50 were investigated in 25 fig cultivars growing in Morocco. The aims of this study were to determine (i) the variation in these compounds among light and dark-colored cultivars, (ii) their partitioning between fruit peel and pulp and (iii) to display network connections among these variables. Twelve phenolic compounds (PCs) were isolated in peel extract versus eight in pulp samples. Anthocyanins, mainly cyanidin-3,5-diglucoside and cyanidin-3-O-rutinoside, were the predominant compounds in peels, where the mean concentrations were 75.90 ± 18.76 and 77.97 ± 18.95 µg/g dw, respectively. On the other hand, (−)-epicatechin and cyanidin-3-O-rutinoside were the major compounds in the pulp extracts, where the mean values were 5.23 ± 4.03 and 9.01 ± 5.67 µg/g dw, respectively. A two-dimensional hierarchically clustered heatmap was applied to the dataset to explore correlations in the dataset and similarities between cultivars, without dimensionality reduction. Results showed that anthocyanins, particularly pelargonidin-3-O-rutinoside, cyanidin-3,5-diglucoside and cyanidin-3-O-rutinoside, were the main contributors to the peels’ free radical scavenging capacity. This capacity was particularly higher in the peel of dark-colored figs compared to the fruit pulp. The local cultivar “INRA 1301” showed the most promising phenolic profile due to its very high levels of almost all detected PCs, especially (−)-epicatechin, quercetin-3-O-rutinoside, quercetin-3-O-glucoside, cyanidine-3,5-diglucoside, cyanidine-3-O-rutinoside and pelargonidin-3-O-rutinoside (54.66, 141.08, 35.48, 494.08, 478.66, 12.56 µg/g dw, respectively). Having the darkest figs in the collection (L* = 25.72, c* = 22.09 and h° = 20.99), this cultivar has also combined promising IC50 values, which were of 19.85, 40.58 and 124.78 µg/mL for DPPH, ABTS and LPIC essays, respectively.

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The influence of different screw tightening forces on the vertical misfit of implant-supported frameworks

Journal of Applied Oral Science ◽

10.1590/s1678-77572005000200005 ◽

2005 ◽

Vol 13 (2) ◽

pp. 120-125 ◽

Cited By ~ 3

Author(s):

Diego Klee de Vasconcellos ◽

Marco Antonio Bottino ◽

Renato Sussumu Nishioka ◽

Luiz Felipe Valandro ◽

Elza Maria Valadares da Costa

Keyword(s):

Repeated Measures ◽

In Vitro Study ◽

Pairwise Comparisons ◽

Tukey Test ◽

Nickel Chromium ◽

Marginal Discrepancy ◽

The Mean ◽

Post Hoc ◽

One Way Anova

OBJECTIVES: The present in vitro study was designed to compare the differences in the vertical misfit of implant-supported frameworks using three different forces for tightening the bridge locking screws: fastening by hand until first resistance, and using torque drivers with 10 and 20Ncm. METHODS: The investigation was conducted based on the results given by 9 six-unit nickel-chromium (2 abutments/ 4 pontics) screw-retained implant-supported frameworks. The structures were exposed to simulated porcelain firings. The marginal misfit measurements were made using a traveling measuring microscope at selected screw tightening forces: fastening by hand until first resistance, and using torque drivers with 10 and 20Ncm. The results were submitted to one-way ANOVA with repeated measures on one factor, and post hoc pairwise comparisons using Tukey test (5%). RESULTS: The mean marginal misfit of the frameworks, fastening the screws by hand until first resistance, was 41.56µm (SD±12.45µm). The use of torque driver devices caused a significant reduction in marginal opening (p<0.05). With the lowest torque available (10Ncm), the mean marginal discrepancy at the abutment-framework interface was reduced an average of 52% to a mean marginal opening of 19.71µm (SD±2.97µm). After the use of the 20Ncm torque driver, the mean marginal discrepancy of the frameworks was reduced an average of 69% to a mean marginal opening of 12.82µm (SD±4.0µm). Comparing the use of torque drivers with 10 and 20 Ncm torque, the means are not significantly different from one another. CONCLUSION: The seating force has an important effect on the vertical misfit measurements, once it may considerably narrow the vertical misfit gaps at the abutment-framework interface, thus leading to a misjudgment of the real marginal situation.

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In Vitro Study on Bone Heating during Drilling of the Implant Site: Material, Design and Wear of the Surgical Drill

Materials ◽

10.3390/ma13081921 ◽

2020 ◽

Vol 13 (8) ◽

pp. 1921 ◽

Cited By ~ 2

Author(s):

Juan Carlos Bernabeu-Mira ◽

Hilario Pellicer-Chover ◽

Miguel Peñarrocha-Diago ◽

David Peñarrocha-Oltra

Keyword(s):

Stainless Steel ◽

Axial Loading ◽

In Vitro Study ◽

Material Design ◽

Diamond Like Carbon ◽

Thermal Variation ◽

Mean Values ◽

Carbon Coated ◽

The Mean

Objective: An in vitro study was made to compare mean thermal variation according to the material, design and wear of the surgical drills used during dental implant site preparation. Material and methods: Three study groups (stainless steel drills with straight blades; diamond-like carbon-coated drills with straight blades; and diamond-like carbon-coated drills with twisted blades) were tested to compare material, design and wear of the surgical drill in terms of overall mean values (complete sequence of drills) and specific mean values (each drill separately). The groups comprised four drills: initial, pilot, progressive and final drill. Implant site configuration was performed through an intermittent and gradual drilling technique without irrigation at 800 rpm in standardized synthetic blocks. Maximum axial loading of two kilograms was controlled by an automatic press. Each surgical drill was submitted to 50 drillings and was sterilized every five uses. A thermographic camera analyzed the mean thermal changes. The software-controlled automatic press kept systematic drilling, axial loading and operational speed constant without any human intervention. Student’s t-test, ANOVA and multiple linear regression models were performed. The level of significance was 5% (p = 0.05). Results: The overall mean comparison between the stainless steel and diamond-like carbon-coated materials showed no statistically significant differences (p > 0.05), though specific mean comparison showed statistically significant differences between the drills of the different groups (p < 0.05). The twisted blades exhibited less overall and specific mean thermal variation than straight blades for the progressive and final drills (p < 0.01). In addition, the initial and pilot drills showed a greater mean thermal change than the progressive and final drills. The mean thermal variation was seen to increase during the 50 drillings. Conclusions: Within the limitations of this study, it can be concluded that the drill material did not significantly influence the overall mean thermal variation except for the pilot drill. The drill design affected overall and specific mean thermal variation since the twisted blades heated less than the straight blades. The initial and pilot drills increased the specific mean thermal variation with respect to the progressive and final drills. In addition, all drills in each group produced a gradual increase in mean temperature during the 50 drillings.

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Comparative in vitro study of the cleaning efficacy of AirFloss ultra and I-Prox Sulcus brushes in an orthodontic phantom model

Scientific Reports ◽

10.1038/s41598-021-81603-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Hanna Boes ◽

Sören Brüstle ◽

Gholamreza Danesh ◽

Stefan Zimmer ◽

Mozhgan Bizhang

Keyword(s):

In Vitro Study ◽

White Spot Lesions ◽

Mean Values ◽

Phantom Model ◽

Before And After ◽

Tooth Surfaces ◽

The Mean ◽

Post Hoc ◽

Cleaning Efficacy

AbstractPreventing biofilm-related risks such as gingivitis and white spot lesions during orthodontic treatments is very challenging. The cleaning efficiencies of AirFloss Ultra and I-Prox P sulcus brushes were evaluated using an orthodontic phantom model. After attaching brackets onto black-coated maxillary KaVo teeth, a plaque substitute was applied. The evaluated tooth surfaces were divided into two areas. Cleaning was performed with an AirFloss Ultra with two (A-2) or four (A-4) sprays or an I-Prox P for two (I-2) or four (I-4) seconds. Images before and after cleaning were digitally subtracted, and the percentage of fully cleaned surfaces was determined (Adobe Photoshop CS5, ImageJ). Statistical analysis was performed by ANOVA and post hoc tests with Bonferroni correction (SPSS 25, p < 0.05). The mean values of total cleaning efficacy were 26.87% for I-2, 43.73% for I-4, 34.93%, for A-2 and 56.78% for A-4. The efficacy was significantly higher for A-4 than for A-2, I-4, and I-2. There were significant differences between the four groups. Repeated cleaning led to an improved result. Within the study limitations, the AirFloss Ultra with four sprays proved to be more efficient than the sulcus brush I-Prox P for cleaning.

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Comparative Evaluation of Marginal Fit of Three Provisional Restorative Materials in Different Mediums: An In-Vitro Study

International Journal of Research and Review ◽

10.52403/ijrr.20210303 ◽

2021 ◽

Vol 8 (3) ◽

pp. 9-14

Author(s):

Manju Choudhary

Keyword(s):

Methyl Methacrylate ◽

Room Temperature ◽

In Vitro Study ◽

Marginal Adaptation ◽

Polymerization Shrinkage ◽

Mean Values ◽

Group A ◽

The Mean ◽

Group B

Temporaries are used as placeholders before the permanent crowns are installed. If the temporary crown is not fitted properly the tooth can be subject to increased decay and gums can become inflamed causing gingivitis which leads to other more serious problems. A provisional fixed restoration will provide a template for defining tooth contour, esthetics, proximal contacts, ridge contacts and occlusion. Margins made by the indirect technique are considered to be more accurate than those made by the direct technique. The purpose of this in vitro study was to compare the marginal accuracy of provisional crowns made from three different biomaterials using the established indirect method in different environments and to evaluate the effect of water absorption on polymerization shrinkage and the effect of polymerization shrinkage occurring in dry storage for a week. Method:Variables used in this study are: a) Revotek LC-light cured composite b) Protemp II c) poly methyl methacrylate. An aluminium master die was machined with dimensions: 5mm length, 10mm gingival diameter, 5 degree taper, and 1mm shoulder then dental stone die was prepared by making an impression of this experimental model using a poly vinyl siloxane material. Both group A and group B consisted of seven specimens each of DPI, ProtempII, Revotek LC. The specimens were evaluated using measuring microscope (Biolux), which had an eyepiece graticule of 1/10th of an mm. The seven specimens in group A (DPI, ProtempII, Revotek LC) were kept in air at room temperature for one week on the dental stone cast and the readings were tabulated and the mean values were obtained. The seven specimens in group B (DPI, ProtempII, and Revotek LC) were kept in water at room temperature for one week and the readings were tabulated in the same manner and the mean values were obtained. Results: Comparative statistics of the mean values of the specimens kept in air at room temperature showed that DPI had the least marginal discrepancies followed by ProtempII, Revotek LC. The values were highly significant (.002) Comparative statistics of the mean values of the specimens kept in water at room temperature showed that DPI had the least marginal discrepancies followed by Revotek LC, ProtempII. The values were highly significant (.009). Conclusion: After one week in air at room temperature and after one week in water at room temperature DPI recorded the minimal marginal discrepancy. When stored in air at room temperature, DPI had the best marginal adaptation. All of the materials showed evidence of continued polymerization shrinkage after storage in air for a week. Water absorption compensated for polymerization shrinkage in DPI and ProtempII whereas Revotek LC was an exception. Keywords: light cured composite, Protemp II-Bis –acryl composite, poly methyl methacrylate, marginal adaptation, provisional restoration.

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The effect of insertion and photopolymerization techniques on microleakage of Class V cavities: a quantitative evaluation

Brazilian Oral Research ◽

10.1590/s1806-83242005000100006 ◽

2005 ◽

Vol 19 (1) ◽

pp. 30-35 ◽

Cited By ~ 1

Author(s):

Fabiana Mantovani Gomes França ◽

Frederico Seidi Hori ◽

Alex José Souza dos Santos ◽

José Roberto Lovadino

Keyword(s):

In Vitro Study ◽

Root Surface ◽

Group Iv ◽

Class V ◽

Mean Values ◽

Group Iii ◽

Soft Start ◽

Light Curing ◽

The Mean

The aim of this in vitro study was to evaluate by spectrophotometry the influence of the incremental technique and progressive light curing in the microleakage of Class V cavities. Forty samples were prepared with class V cylindrical cavities on the buccal root surface of bovine incisive teeth and filled with composite resin (Z250). The samples were divided into four groups: I: cavity was bulk filled and the composite was light cured for 40 seconds; Group II: cavity was bulk filled and a "soft-start" polymerization was used; Group III: cavity was filled with the incremental technique in two coats and light cured for 40 seconds; Group IV: cavity was filled with the incremental technique in two coats and light cured with "soft-start" polymerization. After the restoration, the specimens were thermally stressed for 3,000 cycles in bath at 5 ± 2°C and 55 ± 2°C, protected with nail enamel, colored with 2% methylene blue and cut into sections. These sections were triturated and the dye was recovered with PA ethanol and the supernatant was evaluated. The data were submitted to ANOVA and the results showed the following averages: bulk filled and conventional photopolymerization (I) 0.06075 µg/ml; bulk filled and progressive photopolymerization (II) 0.04030 µg/ml; incremental insertion and conventional photopolymerization (III) 0.04648 µg/ml; incremental insertion and progressive photopolymerization (IV) 0.04339 µg/ml. No significant statistic differences were observed among the mean values. The Degulux "soft-start" equipment probably emits too high initial light intensity to promote progressive photopolymerization.

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Evaluation of Microshear Bond Strength of Self-Adhesive Versus Conventional Flowable Resin Composite to Sound and Affected Dentin: An In Vitro Study

International Journal of Health Sciences and Research ◽

10.52403/ijhsr.20210436 ◽

2021 ◽

Vol 11 (4) ◽

pp. 306-312

Author(s):

Dalia M. Abouelmagd ◽

Hanan K. Abouelseoud

Keyword(s):

Bond Strength ◽

Resin Composite ◽

In Vitro Study ◽

Vitro Study ◽

Flowable Composite ◽

Significant Difference ◽

The Mean ◽

Microshear Bond Strength ◽

Vertise Flow

Objectives: This study aimed to compare the microshear bond strength (µSBS) of self-adhesive flowable composite and conventional bonded flowable composite to sound and caries affected dentin. Materials and Methods: In this in vitro study, a total of freshly extracted 40 human molar teeth were used. The occlusal surface was sectioned to expose dentin for µSBS test. The teeth were randomly divided into 2 groups according to the type of flowable composite used into two groups (n=20): group A: self- adhesive flowable composite (Vertise flow), group B: Filtek Z350-XT flowable composite and one-step self-etch adhesive Adper Easy One. A plastic cylindrical mold was used to construct resin composite cylinders (0.8mm diameter x 1mm length) on the sound and caries affected exposed dentin and filled with flowable composite restorative materials according to manufacturer’s instructions. The samples were then immersed in distilled water at 37°C for 24 hours, and underwent µSBS test at a crosshead speed of 0.5 mm/min. Data were analyzed using Student's t-test to compare between the two materials as well as to compare between sound and caries affected dentin. Results: The mean values of µSBS of Filtek Z-350XT to sound and caries affected were 26.6 and 26.7 MPa respectively. The mean µSBS of Vertise Flow to sound and affected were 13.2 and 17.9 MPa respectively. With sound dentin FiltekZ-350XT showed statistically significant higher µSBS than Vertise flow. With caries affected dentin, there was no statistically significant difference between the two materials. There was no statistically significant difference between µSBS of sound and caries affected dentin within each material. The significance level was set at P ≤ 0.05. Conclusions: Within the limitations of this study, the conventional flowable composite yielded the highest µSBS to sound dentin. There was no statistical significant difference in µSBS of self-adhesive and conventional flowable to caries affected dentin. Key words: Composite Resins; Dentin; microshear.

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Concentration of hydrogen ions in several calcium hydroxide pastes over different periods of time

Brazilian Dental Journal ◽

10.1590/s0103-64402009000500005 ◽

2009 ◽

Vol 20 (5) ◽

pp. 382-388 ◽

Cited By ~ 13

Author(s):

Morgana Eli Vianna ◽

Danila M. Zilio ◽

Caio Cezar Randi Ferraz ◽

Alexandre Augusto Zaia ◽

Francisco José de Souza-Filho ◽

...

Keyword(s):

Calcium Hydroxide ◽

Local Anesthetics ◽

Glass Plate ◽

In Vitro Study ◽

Hydrogen Ions ◽

Sterile Water ◽

The Mean ◽

Almost All ◽

Chlorhexidine Gel

The purpose of this in vitro study was to measure the concentration of hydrogen ions (pH) of calcium hydroxide [(Ca(OH)2] pastes combined with different vehicles over 7 periods of time. The Ca(OH)2 was manipulated with the following vehicles: i: sterile water; ii: iodoform plus sterile water; iii: local anesthetics (Lydocaine 2% with 1: 100,000 epinephrine); iv: polyethyleneglycol; v: glycerin; vi: 2.0% chlorhexidine gel; vii: camphorated paramonochlorophenol (CMCP); viii: (CMCP) + glycerin; and ix: polyethyleneglycol plus CMCP. The pastes were made on a glass plate to toothpaste consistency and the pH was measured at the following times: 5 min, 1, 24, 48 h; 7, 14 and 28 days. The data were statistically analyzed (Kruskal-Wallis at p<0.05). At 5 min, 1 and 24 h, the pH of all tested pastes ranged from 13.05 to 11.16. At 48 h and 7 days the pH of all tested pastes ranged from 11.66 to 8.92. At 14 and 28 days almost all pastes had pH means lower than 10. In conclusion, the mean pH of all tested calcium hydroxide pastes decreased with the time. Pastes made with aqueous vehicles (especially with sterile water), followed by oily vehicles (especially with CMCP + glycerin), held the highest pH means over the periods of time tested.

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Molecular adsorbent recirculating system (MARS) and continuous veno-venous hemodiafiltration (CVVHDF) for diltiazem removal: An in vitro study

The International Journal of Artificial Organs ◽

10.1177/0391398820975041 ◽

2020 ◽

pp. 039139882097504

Author(s):

Nicolas Fabresse ◽

Islam Amine Larabi ◽

Elodie Lamy ◽

Bruno Mégarbane ◽

Jean-Claude Alvarez

Keyword(s):

Initial Dose ◽

In Vitro Study ◽

Central Compartment ◽

Dialysis Membrane ◽

Molecular Adsorbent Recirculating System ◽

The Mean ◽

Almost All ◽

Diffusion Convection ◽

Molecular Adsorbent

The objective of the present study was to evaluate the efficacy of the molecular adsorbent recirculating system (MARS) vs continuous veno-venous hemodiafiltration (CVVHDF). Diltiazem poisoning was simulated in a central compartment consisting in a 5L dialysis solute spiked with diltiazem at two different toxic concentrations: 750 and 5000 µg/L. For CVVHDF, mean extraction coefficients (EC = (in concentration − out concentration)/in concentration) were concentration-dependent with a decrease all along the dialysis. At the end of the sessions the mean amounts remaining in the central compartment were 8% and 7% of the initial dose at 750 and 5000 µg/L, respectively. The mean cumulative amounts found in the effluent were 60% and 75% of the initial dose, respectively. The missing amounts accounted for 32% and 18% of the initial dose, respectively, corresponding to an adsorption to the dialysis membrane. In contrast, the different compartments of the MARS resulted in undetectable output concentration earlier that the end of the session. The mean concentrations of diltiazem remaining in the central compartment were <1 µg/L at the end of the sessions. Global ECs were around 50% all along the experiment at both concentrations, and the average charcoal cartridge ECs was 80% throughout the experiments. CVVHDF system in the developed model was efficient for diltiazem removal, mainly by diffusion, convection and to a lesser extent by adsorption to the dialysis membrane. In MARS system, resin cartridge and hemodialysis components are ineffective, charcoal cartridge is responsible for almost all drug removal.

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