Mastitis in beef cows and the effects of supplemental β-carotene on milk parameters

2010 ◽  
Vol 50 (6) ◽  
pp. 503 ◽  
Author(s):  
D. H. Whiteman ◽  
N. W. Tomkins ◽  
R. J. Young ◽  
I. Immig ◽  
G. Weber ◽  
...  
Keyword(s):  
Treatment Group ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Beef Cows ◽  
Control Group ◽  
Blood Samples ◽  
Milk Samples ◽  
Average Condition ◽  
Ad Libitum ◽  
Β Carotene

Belmont red and Brahman cows (n = 50) were allocated to one of two groups to determine if β-carotene supplementation could reduce the incidence of mastitis and consequently improve calf productivity from birth to weaning. Both groups received a molasses-based supplement ad libitum; the treatment group supplement was fortified with ROVIMIX® β-carotene and ROVIMIX® E50 whereas the control group were provided access to the molasses supplement only. Blood samples were collected from cows pre and post calving and from calves when 84 ± 0.4 days old to measure plasma β-carotene concentration (PCC). Milk samples were collected 7 and 84 days post calving and at weaning and analysed for somatic cell count (SCC) and composition. Supplementation had no significant (P > 0.05) effect on SCC, calf PCC, calf LW gain and LW at weaning. Supplemented cows had significantly (P < 0.05) higher PCC compared with unsupplemented cows (4.9 ± 0.36 v. 3.9 ± 0.24 mg/L, respectively) at weaning. Cows commenced the study in an above-average condition, and combined with unseasonal green forage it is concluded that β-carotene supplementation has no effect on mastitis or calf weaning weight.

scholarly journals Role of Teat Dipping After Milking for Subclinical Mastitis Control and Improving Production of Dairy Cow

2019 ◽  
Vol 43 (2) ◽  
Author(s):  
Ardilasunu Wicaksono ◽  
Etih Sudarnika ◽  
Herwin Pisestyani ◽  
Mirnawati Sudarwanto ◽  
Abdul Zahid ◽  
...  
Keyword(s):  
Treatment Group ◽  
Dairy Cattle ◽  
Somatic Cell ◽  
Cell Count ◽  
Milk Production ◽  
Somatic Cell Count ◽  
Subclinical Mastitis ◽  
Control Group ◽  
Lactation Period ◽  
Teat Dipping

This study was aimed to compare somatic cell count and milk production on dairy cattle after teat dipping post milking application. Samples collected from total of 121 quarters of dairy cattle in normal lactation period and identified as infected by subclinical mastitis. Subclinical mastitis examination was done by IPB Test Mastitis Test, somatic cell count calculation was done by Breed Method, and observation of milk production was done by calculating milk volume per quarter. Observations were performed during normal lactation periods of 12 weeks. Data were analyzed statistically using Mann-Whitney Test and unpaired t-test. The results showed that the number of somatic cells count in the treatment group with post milking teat dipping application was lower than in the control group and significantly different at week 11 (p = 0.039). Furthermore, the volume of milk production in the treatment group was higher than in the control group and significantly different in almost all weeks of observation (p<0.05). It was showed that post milking teat dipping application can prevent subclinical mastitis infection effectively, seen from low somatic cell count (below 400.000 cells/ml) and can maintain optimum milk production. So that, this application can increase the economic benefits for dairy farmers.

scholarly journals Bulk tank somatic cell count and associated microbial quality of milk from selected dairy cattle herds in Oyo State, Nigeria

2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Olufemi Olatoye ◽  
Adesola Amosun ◽  
Uzo Ogbu ◽  
Yemi Okunlade
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Health Management ◽  
Development Program ◽  
Raw Milk ◽  
Human Consumption ◽  
Quality And Safety ◽  
Milk Samples ◽  
California Mastitis Test

Improvement of traditional and nomadic milk production through dairy development program in Nigeria requires routine quality and safety monitoring of milk both at herd level and milk collection centers. A total of 411 bulk raw milk samples aseptically obtained from Ibarapa, Oyo and Oke-Ogun industrial milk collection centers were subjected to California Mastitis Test (CMT), Bulk Somatic Cell Count (BSCC) and bacteriological analysis for assessment of quality and safety of milk from the herds. One hundred and seven (26.0%) of the samples were CMT positive, while 74.0% were negative to CMT. The overall mean BSCC, TAC and TCC were 1.27×103 ± cells/mL, 1.12×103± 34 cfu/mL, 97.8±9.8 cfu/mL in the CMT negative milk samples while for the strong positive samples the mean BSCC, TAC and TCC were 4.33×106 ± cells/mL, 2.35×106 ± 453 cfu/mL, 189.3±41.1 cfu/mL respectively; these were higher than the Pasteurized Milk Ordinance acceptable limits. Positive correlation was found between CMT scores and bacterial contamination and between CMT scores and SCC was recorded. About 26.0% of the samples with positive CMT could be considered unsafe due to strong correlation with microbial contamination that could result in milk borne zoonoses and public health hazards. However, a greater proportion (76.9%) of the milk with negative CMT scores could be safe for human consumption after post-harvest pasteurization. Consequently, there is need to improve handling, environmental and milking hygiene; as well as proper herd and udder health management to improve quality and safety of Nigeria dairy products.

scholarly journals The presence of pathogens in milk of ewes in relation to the somatic cell count and subpopulations of leukocytes

2021 ◽  
Author(s):  
Tvarožková ◽  
Vašíček ◽  
Uhrinčať ◽  
Mačuhová ◽  
Hleba ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Dairy Farm ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Polymorphonuclear Cells ◽  
Coagulase Negative Staphylococci ◽  
Major Health Problem ◽  
Milk Samples

Mastitis is a major health problem of the udder in dairy sheep breeds. For diagnosis of subclinical mastitis, somatic cell count (SCC) is commonly used. The presence of pathogens in the udder causes the increase of leukocytes and thus SCC in milk. Therefore, the aim of this study was to evaluate the presence of pathogens in the milk of ewes and the possible relationship with SCC. The changes of leukocytes subpopulation in milk samples with high SCC were evaluated as well. The experiment was carried out on a dairy farm with the Lacaune breed. This study was conducted on 45 ewes (98 milk samples) without signs of clinical mastitis. Based on somatic cell count, samples were divided to five SCC groups: SCC1 &lt; 200 000 cells/ml (45 milk samples); 200 000 ≤ SCC2 &lt; 400 000 cells/ml (10 milk samples); 400 000 ≤ SCC3 &lt; 600 000 cells/ml (six milk samples); 600 000 ≤ SCC4 &lt; 1 000 000 cells/ml (six milk samples); SCC5 ≥ 1 000 000 cells/ml (31 milk samples). No pathogens were observed in the majority of milk samples (60.20%). Coagulase-negative staphylococci (CNS) were the most commonly isolated pathogens from the milk of ewes (86.11%). Staphylococcus epidermidis had the highest incidence from CNS (35.48%). In the SCC5 group, up to 79.31% of bacteriological samples were positive. The percentage of leukocytes significantly increased (P &lt; 0.001) in the samples with higher SCC (≥ 200 × 10<sup>3</sup> cells/ml) in comparison to the group SCC1. Also, the percentage of polymorphonuclear cells (PMNs) was significantly higher with increasing SCC (P &lt; 0.001). In conclusion, the presented results showed that the high SCC was caused by the presence of the pathogen in milk. Thus SCC &lt; 200 000 cells/ml and leukocyte subpopulation, especially PMNs, could be considered as important tools in udder health programs applied in dairy ewes.

Preservation and Storage Mechanisms for Raw Milk Samples for Use in Milk-Recording Schemes

1996 ◽  
Vol 59 (2) ◽  
pp. 151-154 ◽  
Author(s):  
HUMBERTO G. MONARDES ◽  
ROBERT K. MOORE ◽  
BRIAN CORRIGAN ◽  
YVON RIOUX
Keyword(s):  
Cell Count ◽  
Somatic Cell Count ◽  
Potassium Dichromate ◽  
Dairy Herd ◽  
Raw Milk ◽  
Storage Conditions ◽  
Sample Storage ◽  
Milk Samples ◽  
Whole Process ◽  
And Storage

This study, carried out by the Quebec Dairy Herd Analysis Service, compares (during summer conditions in Quebec) the performance of three types of preservatives for raw milk under four different systems of sample storage: no refrigeration, refrigeration at the laboratory only, refrigeration during transport and at the lab, and complete refrigeration from sampling at the farm to analysis. The objective was to determine the best preservative and storage conditions for protecting milk components during transportation and storage of raw milk samples collected at the farm and sent to a central testing lab for analysis. Milk samples were analyzed at day 3 and at day 7 after sampling to observe the effect of aging. A total of 12,480 samples were collected during the trial. The components studied were percentage of fat and protein and somatic cell count (SCC). In general, samples preserved with bronopol (2-bromo-2-nitropropane-1,3-diol and 2-bromo-2-nitropropanol) in liquid or in microtab tended to give higher readings for fat and protein contents than samples preserved with potassium dichromate. Significantly lower fat values were observed in 7-day-old samples compared to 3-day-old samples. Fat depression was more accentuated in nonrefrigerated samples. Under current methods of handling raw milk samples, refrigeration during the whole process of sampling, transportation, and until analysis, seems an ideal to attain to avoid significant reductions of fat values.

scholarly journals Evolution and factors influencing somatic cell count in raw milk from farms in Viçosa, state of Minas Gerais

2017 ◽  
Vol 39 (4) ◽  
pp. 393
Author(s):  
Leonardo Cotta Quintão ◽  
Adriano França da Cunha ◽  
Lindomar José Bragança ◽  
Kamila Soares Coelho ◽  
Mariana Fonseca Nunes ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Significant Variation ◽  
Somatic Cell Count ◽  
Raw Milk ◽  
Minas Gerais ◽  
Milk Samples ◽  
Factors Influencing ◽  
The Mean ◽  
Structured Questionnaire

The somatic cell count (SCC) of refrigerated raw milk reflects the occurrence of mastitis in the herd and the management of rural property. Therefore, this study aimed to evaluate the SCC of the refrigerated raw milk and its determinant factors in dairy properties of Viçosa, State of Minas Gerais. Monthly, milk samples were taken from 44 cooling tanks during 2012. A structured questionnaire was applied to evaluate the production and management characteristics of the herds. There was a significant variation (p < 0.05) in the mean SCC of the milk, which was lower from June to August. Higher mean SCC and percentages of samples above the limit set by the legislation were observed in months of higher rainfall and ambient temperature (p < 0.05). Mechanical milking, lower milk yield, productivity, inadequate milking procedures, equipment hygiene, and water quality were all factors that influenced (p < 0.05) the SCC of raw refrigerated milk. Milker training is required for production of low SCC milk. 

scholarly journals 380 Effects of estradiol benzoate on reproductive characteristics in beef cows

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 145-145
Author(s):  
Lacey M Dysart ◽  
Caleb O Lemley ◽  
Riley Messman ◽  
Jamie E Larson
Keyword(s):  
Doppler Ultrasonography ◽  
Blood Perfusion ◽  
Beef Cows ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
The Other ◽  
Control Group ◽  
Blood Samples ◽  
Doppler Probe ◽  
Imagej Software

Abstract Ovulation prompted by exogenous GnRH may cause the release of sub-mature oocytes and lead to decreased pregnancy rates. Inclusion of estradiol benzoate (EB) has improved pregnancy rates. The objective was to determine if EB affects blood perfusion of follicles, CL, or uterus, concentrations of estradiol, or incidence of standing estrus. Forty-seven suckled beef cows were synchronized (GnRH+CIDR on d -9, PGF2a+CIDR removal on d -2, expected estrus=d 0). On d -1, 24 of 47 cows received an injection of EB (1mg/2mL) while the other 23 remained as a control group. Ovaries were evaluated via Doppler ultrasonography on d -1, 0, 6, 14, and 21. A laser Doppler probe was used to measure blood perfusion of the uterus on d -1, 0, and 6. Only cows that exhibited estrus (n = 29) were evaluated on d 14 and 21. Images were analyzed via ImageJ software to determine mean area of perfusion. Blood samples were collected on d -1 and 0 and analyzed for concentrations of estradiol using RIA. Using SAS, MIXED and FREQ were used to determine the influence of treatment on blood perfusion and estradiol, and incidence of standing estrus, respectively. Treatment with EB increased (P &lt; 0.001) the number of cows exhibiting standing estrus compared to control (21/24 vs 8/23, respectively). Treatment of EB did not (P &gt; 0.10) alter blood perfusion of the follicles or uterus on any day or perfusion of the CL on d 6. However, EB increased (P &lt; 0.036) the size of the CL on d 14 (25.06±1.7 vs 6.06±3.7) and tended (P &lt; 0.097) to increase the size of the CL on d 21. Treatment of EB increased (P &lt; 0.003) concentrations of estradiol on d 0 compared to control (21.35±4.49 vs 9.44±4.61 pg/mL). In conclusion, treatment with EB may play a role in changing the CL after ovulation.

Relationship of Somatic Cell Count and Total Sulfhydryls in Milk

1986 ◽  
Vol 49 (2) ◽  
pp. 110-111 ◽  
Author(s):  
DANIEL R. SAMPLES ◽  
SUSAN L. DILL ◽  
RONALD L. RICHTER ◽  
CHARLES W. DILL
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Milk Protein ◽  
Cell Concentration ◽  
Chemical Parameters ◽  
Milk Samples ◽  
The Mean ◽  
Relationship Of ◽  
The Relationship

Individual milk samples from 32 cows were analyzed to determine the relationship between somatic cell concentration and total sulfhydryl concentration (cysteine plus reduced cystine). A significant relationship was detected between somatic cell count, which ranged from 1.7 × 104 to 1.0 × 107 cells/ml, and total sulfhydryls per gram of milk protein. The regression equation, total sulfhydryls/g of milk protein = 31.96 + 7.99 (log10 somatic cell count) with r2 = 0.19, was calculated. The mean total sulfhydryl concentration was 73.1 μmol/g of protein. The minimal effect of somatic cell concentration on total sulfhydryl concentration indicates that somatic cell concentration should have little influence on chemical parameters of milk protein determined by sulfhydryl analysis when proper experimental controls are used.

scholarly journals Haptoglobin and serum amyloid A in relation to the somatic cell count in quarter, cow composite and bulk tank milk samples

2007 ◽  
Vol 74 (2) ◽  
pp. 198-203 ◽  
Author(s):  
Maria Åkerstedt ◽  
Karin Persson Waller ◽  
Åse Sternesjö
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Serum Amyloid A ◽  
Dairy Farms ◽  
Serum Amyloid ◽  
Bulk Tank Milk ◽  
Amyloid A ◽  
Milk Samples ◽  
Bulk Tank

Milk somatic cell count (SCC) is the gold standard in diagnosis of subclinical mastitis, and is also an important parameter in quality programmes of dairy cooperatives. As routine SCC analysis is usually restricted to central laboratories, much effort has been invested in the search for alternative biomarkers of mastitis and milk quality, including the presence in the milk of the acute phase proteins (APP), haptoglobin (Hp) and serum amyloid A (SAA). The aim of this study was to investigate relationships between Hp, SAA and SCC in quarter, cow composite, and bulk tank milk samples. Cows (n=165), without any clinical signs of disease or abnormalities in the milk or udder, from three different dairy farms, were used. Cow composite milk samples from all cows delivering milk at the sampling occasion were taken once in each herd. In one of the farms, representative quarter milk samples (n=103) from 26 cows were also collected. In addition, bulk tank milk samples from 96 dairy farms were included in the study. Samples were analysed for Hp, SAA and SCC, and relationships between the parameters were evaluated at quarter, cow and tank milk levels using Chi-square analysis. Milk samples were categorized according to their SCC, and the presence, or no presence, of SAA and Hp, based on the detection limits of the screening methods (0·3 mg/l and 1·0 mg/l for SAA and Hp, respectively). Hp and SAA were found in milk at quarter, cow composite and bulk tank levels. A large proportion (53%) of the animals had detectable milk concentrations of APP, and SAA was detected more frequently, and at higher concentrations than Hp, regardless of sample type. SAA was detected in as many as 82% of the bulk tank milk samples. Significant relationships were found between Hp, SAA and SCC at quarter and cow composite milk levels, but only between SAA and SCC at bulk tank milk level. Detectable levels of APP were more common at high SCC.

Association of milk quality parameters with teat and udder traits in Tharparkar cows

2017 ◽  
Author(s):  
M. Aurif Wagay ◽  
A. K.S. Tomar ◽  
S. A. Lone ◽  
Anup K. Singh ◽  
Carolina P.
Keyword(s):  
Cell Count ◽  
Negative Correlation ◽  
Milk Sample ◽  
Present Experiment ◽  
Somatic Cell Count ◽  
Quality Parameters ◽  
Milk Quality ◽  
Phenotypic Correlation ◽  
Quality Traits ◽  
Milk Samples

The present experiment was carried out to study the association of milk quality traits (fat, protein, lactose, solid not fat (SNF), total solids (TS) and somatic cell count (SCC)) with teat traits ((teat length (TL), teat diameter (TD), distance between teats (DbT) and teat tip height from ground (THFG)) and udder traits ((udder circumference= UC, udder depth (UD), udder width (UW) and udder height from ground (UHFG)), in Tharparkar cows. The experiment was carried out on 32 tharparkar cows of different parities and lactation stages. Morning and evening measurement of teat and udder traits was carried out before milking and approximately 30 ml of representative milk samples were taken from each of the functional quarters and one composite milk sample of each experimental animal fortnightly from the date of calving of the animal in clean sampling bottles. Fat% and SNF% showed a negative, significant (P>0.05) to highly significant (P>0.01) correlation with TD, UL and FUD. Protein% expressed a positive, significant (P>0.05) to highly significant (P>0.01) correlation with UC, FUD and DbT. Further, Fat%, SNF% and TS% expressed a positive, significant (P>0.05) to highly significant (P>0.01) correlation with THFG. SCC (×105/ml) showed a positive, significant (P>0.05) to highly significant (P>0.01) correlation with TL, TD, DbT, UC and UL, whereas it had a negative correlation with THFG, rear-udder depth (RUD) and UW. It was further observed that Fat% expressed a positive highly significant (P>0.01) phenotypic correlation with SNF% and TS%. SNF%, Lactose%, Protein% and TS% expressed a negative phenotypic correlation with SCC.

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