Comparison of enantiomers of organic acids for their effects on methane production in vitro

2014 ◽  
Vol 54 (9) ◽  
pp. 1345 ◽  
Author(s):  
L. G. Reis ◽  
A. V. Chaves ◽  
S. R. O. Williams ◽  
P. J. Moate
Keyword(s):  
Organic Acids ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Ammonia Concentration ◽  
The Other ◽  
In Vitro Fermentation ◽  
Ruminal Microorganisms ◽  
Dry Matter Digestibility ◽  
Ch4 Production

This study aimed to evaluate the effect of organic acids on in vitro fermentation characteristics. Four organic acids (tartaric, malic, fumaric and citric) and their enantiomers (L-tartaric, D-tartaric, DL-tartaric, L-malic and DL-malic) were analysed using in vitro batch culture incubations, at four concentrations (0, 5, 10 and 15 mM). Cumulative total gas and methane (CH4) production (mL/g DM) were measured at 6, 12 and 24 h; ammonia, pH, volatile fatty acids (VFA) and in vitro dry matter digestibility (IVDMD) were determined after 24 h of fermentation. Overall, addition of acids at 5 to 15 mM increased (P < 0.0001) cumulative gas and CH4 production. No effect (P > 0.10) of enantiomers, individual acid or interaction acid × concentration was detected at 12 and 24 h for cumulative gas or CH4 production. Addition of DL-malic, L-malic and fumaric acids increased (P < 0.0001) the percentage of propionic acid in the ruminal fluid total VFA compared with all concentrations of the other organic acids or their enantiomers. Ammonia concentration was not affected (P ≥ 0.28) by the addition of organic acids, concentrations or interactions. These findings are evidence that ruminal microorganisms can metabolise both D- and L-enantiomers of organic acids. None of the organic acids and their enantiomers at four different concentrations demonstrated potential as CH4 mitigation agents.

scholarly journals Effects of Kikuyu grass (Pennisetum clandestinum) age and different forage: concentrate ratios on methanogenesis

2015 ◽  
pp. 4726-4738 ◽  
Author(s):  
John Ramírez ◽  
Sandra Posada O ◽  
Ricardo Noguera
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Gas Production ◽  
Repeated Measure ◽  
Pennisetum Clandestinum ◽  
Dry Matter Digestibility ◽  
Ch4 Production ◽  
Kikuyu Grass

ABSTRACT Objective. To evaluate the effect of Kikuyu grass (Pennisetum clandestinum) harvested at two different ages and three forage: concentrate supplement ratios (F/C) on methane (CH4) production, dry matter digestibility (DMD), and fermentation profile using the in vitro gas production technique. Materials and methods. six treatments, resulting from the combination of pasture age (30 or 60 days) and F/C (100/0, 75/25, or 50/50) were evaluated using a 2x3 factorial design. The response variables were measured 6, 12, 24 and 48 hours after incubation. A repeated-measure over time design was used to analyze the data, and differences between means were determined with the LSMEANS procedure of SAS. Results. the youngest grass (30 days) was more digestible, produced less CH4 per gram of digestible dry matter (dDM) and more total volatile fatty acids (VFA) compared to the oldest grass (60 days; p <0.05). Reductions of the F/C ratio increased DMD and CH4 production per gram of dDM (p<0.05) but had no significant effect on VFA concentration (p>0.05). Conclusions. under in vitro conditions and pH close to neutrality, the older grass reduces DMD and increases CH4 production per gram of dDM, while a F/C reduction increases DMD and CH4 production per gram of dDM, which differs with reports conducted in vivo.

scholarly journals Effects of Geraniol and Camphene on in Vitro Rumen Fermentation and Methane Production

2017 ◽  
Vol 48 (2) ◽  
pp. 63-69
Author(s):  
M. Joch ◽  
V. Kudrna ◽  
B. Hučko
Keyword(s):  
Methane Emission ◽  
Methane Production ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Positive Control ◽  
Rumen Fermentation ◽  
Dry Matter Digestibility

AbstractThe objective of this study was to determine the effects of geraniol and camphene at three dosages (300, 600, and 900 mg l-1) on rumen microbial fermentation and methane emission in in vitro batch culture of rumen fluid supplied with a 60 : 40 forage : concentrate substrate (16.2% crude protein, 33.1% neutral detergent fibre). The ionophore antibiotic monensin (8 mg/l) was used as positive control. Compared to control, geraniol significantly (P < 0.05) reduced methane production with increasing doses, with reductions by 10.2, 66.9, and 97.9%. However, total volatile fatty acids (VFA) production and in vitro dry matter digestibility were also reduced (P < 0.05) by all doses of geraniol. Camphene demonstrated weak and unpromising effects on rumen fermentation. Camphene did not decrease (P > 0.05) methane production and slightly decreased (P < 0.05) VFA production. Due to the strong antimethanogenic effect of geraniol a careful selection of dose and combination with other antimethanogenic compounds may be effective in mitigating methane emission from ruminants. However, if a reduction in total VFA production and dry matter digestibility persisted in vivo, geraniol would have a negative effect on animal productivity.

scholarly journals Effect of Ligninolytic Axenic and Coculture White-Rot Fungi on Rice Straw Chemical and In Vitro Fermentation Characteristics.

2021 ◽  
Author(s):  
Osmond Datsomor ◽  
Zhao Gou-qi ◽  
Lin Miao
Keyword(s):  
Rice Straw ◽  
Volatile Fatty Acids ◽  
Nutritive Value ◽  
Lignin Content ◽  
Axenic Culture ◽  
White Rot Fungi ◽  
White Rot ◽  
In Vitro Fermentation ◽  
Dry Matter Digestibility

Abstract The objective of this study was to investigate the axenic culture of Pleurotus ostreatus, Phanerochaete chrysosporium, and the coculture (P. chrysosporium and P. ostreatus) for their potential to break down lignin and to enhance the rumen fermentability of rice straw. Rice straw was fermented by two lignin-degrading fungi, namely, P. ostreatus, P. chrysosporium, and coculture (P. ostreatus and P. chrysosporium) under solid-state fermentation (SSF). The coculture exhibited a mutual intermingling plus inhibition interaction. Coculture treated straw had a lower lignin content (5.26%) compared to P. chrysosporium axenic treated straw (6.18%), although P. ostreatus axenic treated straw was the lowest (3.27%). The polysaccharide content of coculture treated straw was higher than P. chrysosporium axenic treated straw, although smaller than P. ostreatus. P. ostreatus and coculture suitably delignified rice straw without adversely affecting cellulose. Treatment of rice straw with coculture improved in vitro dry matter digestibility (68.08%), total volatile fatty acids (35.27%), and total gas (57.4 ml/200 mg) compared to P. chrysosporium (45.09%, 32.24%, 44.39 ml/200 mg) but was second to P. ostreatus (75.34%, 38.31%, 65.60 ml/200 mg). The coculture via synergistic interaction has the potential to decompose lignin and improve the nutritive value of rice straw than P. chrysosporium.

scholarly journals Dose response of biochar and wood vinegar on in vitro batch culture ruminal fermentation using contrasting feed substrates

2021 ◽  
Author(s):  
G C O’Reilly ◽  
Y Huo ◽  
S J Meale ◽  
A V Chaves
Keyword(s):  
Batch Culture ◽  
Dose Response ◽  
Dry Matter ◽  
Maize Silage ◽  
Enteric Fermentation ◽  
Wood Vinegar ◽  
Dry Matter Digestibility ◽  
Ch4 Production ◽  
Fermentation Parameters

Abstract Within Australia, approximately 6.4% of total greenhouse gas emissions are from animal methane (CH4) derived from enteric fermentation. Mitigation of ruminant CH4 is a key concept in support of sustainable agriculture production; dietary manipulations a viable strategy to lower CH4 release during enteric fermentation. In order to determine the effects of dose response of biochar and wood vinegar supplementation on fermentation parameters and CH4 production, this study utilized in vitro batch culture incubations. It is hypothesized the addition of either biochar or wood vinegar will successfully reduce enteric CH4 emissions without negative modification of other fermentation parameters. Three feed substrates (vegetable mixed ration, maize silage and winter pasture) were separated into treatments containing either biochar at 0%, 0.5%, 1%, 2% and 4% DM replacing substrate (w/w basis), or wood vinegar at 0%, 0.25%, 0.5%, 1% and 2% into incubation media volume (v/v). At 6, 12 and 24 hours after inoculation, total gas volume and methane (CH4 %) were measured. Volatile fatty acids (VFA) concentrations, media pH and in vitro dry matter digestibility were measured at 24h. Biochar at various dosages had no effect (P &gt; 0.05) on fermentation characteristics other than decreased in vitro dry matter digestibility (IVDMD; P = 0.01) at 2 and 4% (DM basis) inclusion. Similar to biochar, dose response of wood vinegar had no effect on in vitro fermentation characteristics. However, feed substrate had major effects on all fermentation parameters (P = 0.01) where winter pasture &gt; vegetable mixed ration &gt; maize silage for all recorded fermentation characteristics. Biochar and wood vinegar supplementation were ineffectual in mitigating CH4 production or modifying fermentation characteristics, thus rejecting the initial hypothesis. These results suggest the use of biochar is not an effective tool for methane mitigation in ruminant livestock and infers that studies previously reporting success must better define the systemic mechanisms responsible for the reduction in CH4.

Experiments on rumen retention time, fermentation rate and dry-matter digestibility in zebu and european-type cattle on a grass hay ration

1960 ◽  
Vol 54 (3) ◽  
pp. 417-420 ◽  
Author(s):  
G. D. Phillips ◽  
R. E. Hungate ◽  
A. MacGregor ◽  
D. P. Hungate
Keyword(s):  
Retention Time ◽  
Dry Matter ◽  
Rumen Fermentation ◽  
The Other ◽  
Fermentation Products ◽  
Fermentation Rate ◽  
Dry Matter Digestibility ◽  
Two Factors ◽  
High Level

1. Experiments are described in which retention time of digesta in the reticulo-rumen, fermentation rates of rumen contents, and dry-matter digestibilities were studied simultaneously in four grade European and three zebu steers.2. Fermentation rates and rumen retentions were significantly negatively correlated.3. Correlations between digestibility and the other two factors were not significant at a high level.4. The multiple regressions calculated for retention time and fermentation rate were significant at the 5% level and that for digestibility approached this level.5. While only fermentation rates show significant differences for the two types of cattle, the results suggest that grades and zebus differ also in the rate of passage of digesta through the rumen.6. The loss in weight of substrate per unit of fermentation products was measured inin vitroexperiments.7. Using certain assumptions, estimates are made of the extent to which the measured fermentation rates could account for the loss in weight of dry matter during digestion, and are compared with the loss actually found.

scholarly journals Dose-response effects of sage (Salvia officinalis) and yarrow (Achillea millefolium) essential oils on rumen fermentation in vitro

2018 ◽  
Vol 18 (1) ◽  
pp. 125-142
Author(s):  
Mina Kahvand ◽  
Mostafa Malecky
Keyword(s):  
Essential Oil ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Dose Range ◽  
Ammonia Concentration ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Total Volatile ◽  
Alpha Pinene

Abstract This study aimed at determining the chemical composition of sage essential oil (SEO) and yarrow essential oil (YEO), and investigate in vitro their impacts on gas production kinetics, ruminal digestibility and fermentation, and rumen methanogenesis at different dosages (0, 250, 500 and 750 mg L-1 for SEO; and 0, 250, 500, 750 and 1000 mg L−1 for YEO). Alpha-pinene and 1,8 cineol were two major constituents of both SEO and YEO. Both SEO and YEO had a linear and quadratic effect on asymptotic gas production (P<0.05). The gas production rate increased linearly with SEO and curve-linearly with YEO dosages (P<0.05). In vitro degradability of dry matter and organic matter decreased only by YEO. The partitioning factor (PF) and the microbial biomass (MB) decreased and increased linearly with YEO and SEO dosages, respectively (P<0.05). Total volatile fatty acids (VFA ) were not affected by SEO, but decreased in a linear and quadratic manner with YEO dosage (P<0.05). The VFA pattern was modified in a linear and quadratic manner by both SEO and YEO (P<0.05). Ammonia concentration increased linearly only with YEO increasing doses. The methane to total gas (TG) ratio decreased quadratically only by SEO with reductions of 6.7, 13 and 4.2% at the doses of 250, 500 and 750 mg L−1, respectively. These results revealed that SEO modifies the rumen fermentation positively towards producing more MB and less methane in the dose range of 0-750 mg L−1, however, YEO adversely affected the rumen fermentation at all the tested doses.

scholarly journals Failure of cellulolysis in the rumen of reindeer fed timothy silage

Rangifer ◽  
1995 ◽  
Vol 15 (2) ◽  
pp. 79 ◽  
Author(s):  
Monica A. Olsen ◽  
Tove H. Aagnes ◽  
Svein D. Mathiesen
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rangifer Tarandus ◽  
Initial Period ◽  
Phleum Pratense ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Dry Matter Digestibility ◽  
Low Rate

Three male reindeer (Rangifer tarandus tarandus) calves were brought from mountain pastures in April and fed regrowth timothy (Phleum pratense) silage with 76% leaves and 24.0% dry matter (DM) ad libitum. The silage contained (on DM basis) 25.4% cellulose, 12.0% crude protein and 19-6% water soluble carbohydrates. After an initial period of 11 days the daily silage intake rose to almost similar values for all animals, but independently of food intake, body mass (BM) increased by as much as 13.3 kg for animal R3 during the first 21 days, compared to 4.4 kg and 2.8 kg for Rl and R2, respectively. At slaughter the wet weight of the rumen contents of animal R3 constituted 30.2% of the total BM, compared to 18.5% and 19.1% in animals Rl and R2, respectively. A reduced ability of the rumen micro-biota to ferment pure cellulose in vitro was observed in R3. The ruminal pH was 7.07 and the concentration of volatile fatty acids was only 50.0 mM in R3, indicating a low rate of fermentation. The initial rates of in vitro dry matter digestibility of timothy silage and standard hay were also affected by the rumen fermentation failure in animal R3. Depressed rumen cellulolysis, which may be related to natural periods of starvation prior to the feeding experiment, could have caused the low rate of fermentation and the large rumen size observed in this animal.

scholarly journals Fermentabilitas dan Kecernaan Ransum Domba yang Mengandung Limbah Roti secara In Vitro

2019 ◽  
Vol 19 (2) ◽  
pp. 85-90
Author(s):  
Asep Saripudin ◽  
Shena Nurpauza ◽  
Budi Ayuningsih ◽  
Iman Hernaman ◽  
Ana Rochana Tarmidi
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Native Grass ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Close Relationship ◽  
Completely Randomized Design ◽  
Organic Matter Digestibility

ABSTRAK. Penelitian bertujuan untuk mengetahui fermentabilitas dan kecernaan ransum domba yang mengandung limbah roti. Penelitian menggunakan rancangan acak lengkap dengan lima macam ransum perlakuan yang terdiri atas 40% rumput lapangan dan 60% konsentrat yang masing-masing mengandung limbah roti sebanyak 0 (kontrol), 10, 20, 30, dan 40%. Masing-masing perlakuan diulang sebanyak 4 kali dan data yang terkumpul dianalisis dengan analisis sidik ragam dan dilanjutkan dengan uji Duncan. Ransum perlakuan dievaluasi secara in vitro. Peubah yang diukur adalah asam lemak terbang (ALT), N-NH3, kecernaan bahan kering, dan kecernaan bahan organik. Hasil penelitian menunjukkan bahwa penggunaan limbah roti dalam ransum domba sampai 40% mampu meningkatkan konsentrasi ALT (102,63 - 143,88 mM), kecernaan bahan kering (64,66 - 78,61%) dan kecernaan bahan organik (53,41 - 65,82%). Sementara itu, terjadi kenaikan konsentrasi N-NH3 (3,87 - 4,90 mM) sampai penggunaan limbah roti 30%, namun penggunaan 40% limbah roti sama dengan ransum kontrol. Konsentrasi ALT memiliki hubungan erat dengan kecernaan bahan kering dan bahan organik dengan nilai r = 0,67 dan 0,65, pada persamaan regresi Y = 0,1591X + 50,79 dan Y = 0,1528X + 39,619. Kesimpulan, Penggunaan limbah roti di dalam ransum sebanyak 40% tidak mengganggu fermentabilitas dan kecernaan ransum secara in vitro.  (In vitro fermentability and digestibility of sheep rations containing bread waste)ABSTRACT. The study aimed to determine the fermentability and digestibility of sheep rations containing bread waste. The study used a completely randomized design with five types of treatment rations consisting of 40% native grass and 60% concentrate and each treatment containing bread waste as much as 0 (control), 10, 20, 30, and 40%. Each treatment was repeated 4 times and the data collected was analyzed by analysis of variance and continued by Duncan test. The treatment ration was evaluated by in vitro. The variables measured were volatile fatty acids (VFA), N-NH3, dry matter digestibility, and organic matter digestibility. The results showed that the use of bread waste in sheep rations up to 40% was able to increase the concentration of VFA (102.63 to 143.88 mM), dry matter (64.66 to 78.61%) and organic matter digestibility (53.41 to 65.82%). Meanwhile, there was an increase in the concentration of N-NH3 (3.87 to 4.90 mM) until 30%, but the use of 40% bread waste was the same as the control ration. The VFA concentration has a close relationship with the dry matter and organic matter digestibility with values of r = 0.67 and 0.65, in the regression equation Y = 0.1591X + 50.79 and Y = 0.1528X + 39.619. The conclusion is using of bread waste as much as 40% did not disturb the fermentability and digestibility of the ration in vitro.

The production and absorption of volatile fatty acids from the rumen of the sheep

1968 ◽  
Vol 19 (5) ◽  
pp. 791 ◽  
Author(s):  
GJ Faichney
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Butyric Acid ◽  
Volatile Fatty Acids ◽  
Metabolizable Energy ◽  
The Other ◽  
In Vitro Fermentation ◽  
The Mean ◽  
The Difference

Experiments are reported in which sheep were given roughage diets or a high concentrate diet and the VFA absorbed from the rumen were estimated by an in vitro fermentation procedure. The VFA absorbed were compared with the digestible and metabolizable energy intakes of the sheep, determined in digestibility trials, for each diet. For a lucerne diet, a straw diet, and the high concentrate diet the proportions of the digested energy absorbed as VFA were 33.6, 42.4, and 33.2% respectively. On the lucerne diet, the difference between the mean molar proportions of the VFA absorbed and the mean molar proportions of the VFA in the rumen approached significance for acetic acid (P < 0.10) and was highly significant for butyric acid (P < 0.01). The differences were not significant for the other diets.

scholarly journals Effects of tannins and Monensin in a feedlot diet on in vitro ruminal fermentation

2019 ◽  
Vol 40 (6Supl2) ◽  
pp. 3223
Author(s):  
Hariany Ferreira Martello ◽  
Nelcino Francisco de Paula ◽  
Bruna Gomes Macedo ◽  
Joanis Tilemahos Zervoudakis ◽  
Danielle Dias Brutti ◽  
...  
Keyword(s):  
Volatile Fatty Acid ◽  
Dry Matter ◽  
Low Dose ◽  
Ammonia Concentration ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Ruminal Fermentation ◽  
Kinetics Parameters ◽  
Dry Matter Digestibility

The objective of this study was to evaluate the effects of tannins versus Monensin on in vitro ruminal fermentation of a feedlot diet. The treatments were: control (no additives); low tannin (2 mg g DM-1); medium tannin (4 mg g DM-1), high tannin (6 mg g DM-1), and Monensin (0.02 mg g DM-1). The substrate was a feedlot diet composed by hay and concentrate (15:85 w/w; DM basis). Ruminal fluid was obtained from three rumen-cannulated male Santa Inês sheep. In vitro incubations were carried out during four consecutive weeks (run). Gas production (GP) was recorded at 1, 2, 3, 4, 5, 6, 8, 10, 12, 18, 24, 30, 36, 42, 48, 60, 72, 84, and 96 h of incubation. At 48 and 96 h, two bottles per treatment were withdrawn to measure pH, ammonia concentration (NH3), volatile fatty acid (VFA), in vitro dry matter digestibility (IVDMD), and in vitro neutral detergent fiber digestibility (IVNDFD). Addition of tannin or Monensin did not affect (P > 0.05) the kinetics parameters. Tannin supplementation reduced (P < 0.05) the GP at 24 h compared to Monensin. Addition of Monensin decreased (P < 0.05) IVDMD at 96 h and IVNDFD at 48 and 96 h compared to the control. The IVNDFD was lower (P < 0.05) with Monensin than with tannin at 48 and 96 h. The NH3 was lower (P < 0.05) with tannin compared with Monensin. By increasing tannin dosage, NH3 levels changed quadratically (P < 0.05). The inclusion of tannin in vitro reduced the NH3 concentration considerably when used in low dose.

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