Prioritisation of candidate genes in QTL regions for seed germination and early seedling growth in bread wheat (Triticum aestivum) under salt-stress conditions

2021 ◽  
Vol 72 (1) ◽  
pp. 1
Author(s):  
Elham Rezaei ◽  
Eslam Majidi Hervan ◽  
Amin Azadi ◽  
Alireza Etminan ◽  
Hossein Ramshini
Keyword(s):  
Triticum Aestivum ◽  
Salt Stress ◽  
Candidate Genes ◽  
Metabolic Process ◽  
Stress Conditions ◽  
Phenotypic Variance ◽  
Fresh Weight ◽  
Main Effect ◽  
Early Seedling ◽  
Go Terms

Salinity and drought are major abiotic stresses affecting wheat (Triticum aestivum L.) production throughout the world, and discovery of loci for traits affecting yield under salinity may lead to the breeding for salt-tolerant plants. In the present study, 186 F10 recombinant inbred line (RIL) populations were evaluated under salt-stress conditions in order to identify main-effect and epistatic-effect quantitative trait loci (QTLs) for 15 traits in wheat during the germination and early-seedling stages. In total, 61 main-effect QTLs on 15 chromosomes and 21 epistatic interactions on 12 chromosomes were detected through composite interval mapping (CIM) and a mixed-model-based CIM method. Two major QTLs for primary-leaf fresh weight and coleoptile fresh weight were detected on chromosome (or linkage group) 5B2 and 2D, respectively, which contributed ~44% and 43% of the phenotypic variance. Additionally, 12 QTL clusters including different traits were detected on 1A1, 3A, 4A, 2B1, 3B, 5B1 and 2D1. Candidate genes were identified within QTL regions and gene ontology (GO) enrichment analysis was performed. In total, 9134 candidate genes were grouped into 274 GO terms (including 79 GO terms involved in the ‘biological process’ category). These genes directly or indirectly play a vital role such as lipid localisation, biological regulation, fatty acid biosynthetic process, cellular process, DNA conformation change, translational elongation, carbohydrate metabolic process, Fe ion homeostasis, hydrogen peroxide metabolic process, and pigment biosynthetic process at the germination and early-seedling stages under salt-stress conditions.

QTL Mapping for Seedling Dry Weight and Fresh Weight under Salt Stress and Candidate Genes Analysis in Brassica napus L.

2017 ◽  
Vol 43 (2) ◽  
pp. 179
Author(s):  
Lin-Tao HOU ◽  
Teng-Yue WANG ◽  
Hong-Ju JIAN ◽  
Jia WANG ◽  
Jia-Na LI ◽  
...  
Keyword(s):  
Salt Stress ◽  
Brassica Napus ◽  
Qtl Mapping ◽  
Candidate Genes ◽  
Dry Weight ◽  
Fresh Weight ◽  
Brassica Napus L

scholarly journals Bioprospecting desert plant Bacillus endophytic strains for their potential to enhance plant stress tolerance

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Ameerah Bokhari ◽  
Magbubah Essack ◽  
Feras F. Lafi ◽  
Cristina Andres-Barrao ◽  
Rewaa Jalal ◽  
...  
Keyword(s):  
Salt Stress ◽  
Plant Growth ◽  
Growth Promotion ◽  
Stress Conditions ◽  
Desert Plant ◽  
Plant Growth Promoting Bacteria ◽  
Fresh Weight ◽  
Pgp Traits ◽  
Plant Growth Promoting ◽  
Growth Promoting

AbstractPlant growth-promoting bacteria (PGPB) are known to increase plant tolerance to several abiotic stresses, specifically those from dry and salty environments. In this study, we examined the endophyte bacterial community of five plant species growing in the Thar desert of Pakistan. Among a total of 368 culturable isolates, 58 Bacillus strains were identified from which the 16 most divergent strains were characterized for salt and heat stress resilience as well as antimicrobial and plant growth-promoting (PGP) activities. When the 16 Bacillus strains were tested on the non-host plant Arabidopsis thaliana, B. cereus PK6-15, B. subtilis PK5-26 and B. circulans PK3-109 significantly enhanced plant growth under salt stress conditions, doubling fresh weight levels when compared to uninoculated plants. B. circulans PK3-15 and PK3-109 did not promote plant growth under normal conditions, but increased plant fresh weight by more than 50% when compared to uninoculated plants under salt stress conditions, suggesting that these salt tolerant Bacillus strains exhibit PGP traits only in the presence of salt. Our data indicate that the collection of 58 plant endophytic Bacillus strains represents an important genomic resource to decipher plant growth promotion at the molecular level.

scholarly journals Callus Induction, Proliferation, and Plantlets Regeneration of Two Bread Wheat (Triticum aestivum L.) Genotypes under Saline and Heat Stress Conditions

ISRN Agronomy ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Laid Benderradji ◽  
Faiçal Brini ◽  
Kamel Kellou ◽  
Nadia Ykhlef ◽  
Abdelhamid Djekoun ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Salt Stress ◽  
Bread Wheat ◽  
Heat Tolerance ◽  
Callus Induction ◽  
Thermal Stresses ◽  
Mature Embryo ◽  
Stress Conditions ◽  
Base Line

Response of two genotypes of bread wheat (Triticum aestivum), Mahon-Demias (MD) and Hidhab (HD1220), to mature embryo culture, callus production, and in vitro salt and heat tolerance was evaluated. For assessment of genotypes to salt and heat tolerance, growing morphogenic calli were exposed to different concentrations of NaCl (0, 5, 10, and 15 g·L−1) and under different thermal stress intensities (25, 30, 35, and 40°C). Comparison of the two genotypes was reported for callus induction efficiency from mature embryo. While, for salt and heat tolerance, the proliferation efficiency, embryonic efficiency, and regeneration efficiency were used. The results show significant medium and genotype effects for the embryogenesis capacity of calluses induction and plantlets regeneration under saline and thermal stresses. Mahon-Demias showed good callus induction and ability to proliferate and regenerate seedling under heat and salt stress conditions compared to Hidhab. No sizeable differences were observed between the two genotypes at higher salt stress rates. This study will serve as a base line for in vitro screening of several elite wheat cultivars for their ability to induce callus and regenerate plants from mature embryos, and to start selection for tolerance to salinity.

QTL Mapping of Yield and Yield Components under Normal and Salt-stress Conditions in Bread Wheat (Triticum aestivum L.)

2014 ◽  
Vol 33 (1) ◽  
pp. 102-120 ◽  
Author(s):  
Amin Azadi ◽  
Mohsen Mardi ◽  
Eslam Majidi Hervan ◽  
Seyed Abolghasem Mohammadi ◽  
Foad Moradi ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Salt Stress ◽  
Qtl Mapping ◽  
Bread Wheat ◽  
Yield Components ◽  
Triticum Aestivum L ◽  
Stress Conditions ◽  
Yield And Yield Components

scholarly journals The application of biostimulants Regoplant and Stimpo to increase wheat resistance to salinity conditions

2021 ◽  
Vol 28 ◽  
pp. 117-122
Author(s):  
V. A. Tsygankova ◽  
S. I. Spivak ◽  
O. M. Shysha ◽  
N. L. Pastukhova ◽  
A. I. Yemets ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Salt Stress ◽  
Winter Wheat ◽  
Blot Hybridization ◽  
Triticum Aestivum L ◽  
Physiological Parameters ◽  
Stress Conditions ◽  
Dot Blot ◽  
Free System ◽  
Wheat Embryo

aim. Investigation of bioprotective action of polycomponent biostimulants of natural origin Regoplant and Stimpo according to physiological and molecular genetic parameters of resistance of winter wheat plants (Triticum aestivum L.) varieties Antonivka ta Hoduval'nytsya odes'ka to the salinity conditions. Methods. The physiological parameters of resistance of experimental wheat plants obtained from seeds treated and untreated with solutions of Regoplant and Stimpo biostimulants used at concentrations of 50 μl/L and 75 μl/L grown under salt stress on 0,1 M sodium chloride solution were studied. Using Dot blotting method the degree of hybridization between cytoplasmic si/miRNA isolated from cells of experimental wheat plants grown under salinity conditions and mRNA of control plants grown in the absence of salinity conditions was determined. In the wheat embryo cell-free system of protein synthesis in vitro the silencing activity of si/miRNA on the template of mRNA isolated from cells of experimental wheat plants grown under salt stress conditions and control plants was studied. Results. It was found that the use of biostimulants Regoplant and Stimpo by soaking the seeds of wheat plants for 24 hours at concentrations of 50 μl/L and 75 μl/L improved the physiological parameters of wheat plants grown under salt stress conditions, which increased on average: seed germination – up to 69-75%, the length of seedlings – up to 28-37%, the length of roots – up to 41-42%, leaf area – up to 25-38%, the raw weight of seedlings – up to 48-53%, the dry weight of seedlings – up to 37-46 %, respectively, compared to control. The Dot-blot hybridization method showed that the largest decrease in the percentage of hybridized mRNA and si/miRNA molecules was observed in experimental wheat plants grown from seeds treated with Regoplant and Stimpo biostimulants under salt stress conditions - up to 66-75%, respectively, compared to control. The increase of silencing activity of si/miRNA on the template of mRNA isolated from experimental wheat plants obtained from seeds treated with Regoplant and Stimpo biostimulants and grown under salt stress conditions was shown to be 39-42%, respectively, compared to control. Conclusions. The use of biostimulants Regoplant and Stimpo in concentrations of 50 μl/L and 75 μl/L for presowing seed treatment contributes to the increase of the resistance of winter wheat plants to salinity conditions. Keywords: Triticum aestivum L., polycomponent biostimulants, resistance of wheat to salinity conditions, RNA interference.

scholarly journals Genotyping-by-Sequencing Based Genetic Mapping Identified Major and Consistent Genomic Regions for Productivity and Quality Traits in Peanut

2021 ◽  
Vol 12 ◽  
Author(s):  
Mangesh P. Jadhav ◽  
Sunil S. Gangurde ◽  
Anil A. Hake ◽  
Arati Yadawad ◽  
Supriya S. Mahadevaiah ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Oil Quality ◽  
Genotyping By Sequencing ◽  
Phenotypic Variance ◽  
Potential Candidate ◽  
Quality Traits ◽  
Single Marker Analysis ◽  
Main Effect ◽  
Genomic Regions ◽  
Main Effect Qtls

With an objective of identifying the genomic regions for productivity and quality traits in peanut, a recombinant inbred line (RIL) population developed from an elite variety, TMV 2 and its ethyl methane sulfonate (EMS)-derived mutant was phenotyped over six seasons and genotyped with genotyping-by-sequencing (GBS), Arachis hypogaea transposable element (AhTE) and simple sequence repeats (SSR) markers. The genetic map with 700 markers spanning 2,438.1 cM was employed for quantitative trait loci (QTL) analysis which identified a total of 47 main-effect QTLs for the productivity and oil quality traits with the phenotypic variance explained (PVE) of 10–52% over the seasons. A common QTL region (46.7–50.1 cM) on Ah02 was identified for the multiple traits, such as a number of pods per plant (NPPP), pod weight per plant (PWPP), shelling percentage (SP), and test weight (TW). Similarly, a QTL (7.1–18.0 cM) on Ah16 was identified for both SP and protein content (PC). Epistatic QTL (epiQTL) analysis revealed intra- and inter-chromosomal interactions for the main-effect QTLs and other genomic regions governing these productivity traits. The markers identified by a single marker analysis (SMA) mapped to the QTL regions for most of the traits. Among the five potential candidate genes identified for PC, SP and oil quality, two genes (Arahy.7A57YA and Arahy.CH9B83) were affected by AhMITE1 transposition, and three genes (Arahy.J5SZ1I, Arahy.MZJT69, and Arahy.X7PJ8H) involved functional single nucleotide polymorphisms (SNPs). With major and consistent effects, the genomic regions, candidate genes, and the associated markers identified in this study would provide an opportunity for gene cloning and genomics-assisted breeding for increasing the productivity and enhancing the quality of peanut.

Effect of exogenous nitric oxide (NO) supply on germination and seedling growth of mungbean (cv. Nm-54) under salinity stress

2017 ◽  
Author(s):  
Muhammad Salahuddin ◽  
Fahim Nawaz ◽  
Muhammad Shahbaz ◽  
Muhammad Naeem ◽  
Bilal Zulfiqar ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Seedling Growth ◽  
Dry Matter ◽  
Germination Percentage ◽  
Stress Conditions ◽  
Tolerance Index ◽  
Fresh Weight ◽  
Chlorophyll Contents ◽  
Exogenous Nitric Oxide

The present study evaluated the effects of exogenous NO supply, using sodium nitroprusside (SNP) as a source of NO, on germination and seedling growth of mungbean (Vigna radiata) under salt stress conditions. The results showed that the seeds treated with NO solution (0.2 mM SNP) exhibited 80% and 109% higher germination percentage and germination stress tolerance index (GSI) than untreated seeds (control) under salt stress conditions. Similarly, the seedlings fertigated with NO maintained the highest values of 77.8%, 84.3%, 77.2%, 60.5% and 100.3% for plant height stress tolerance index (PHSI), root length stress tolerance index (RLSI), shoot fresh weight stress tolerance index, root fresh weight stress tolerance index (RFSI) and dry matter stress tolerance index (DMSI), respectively. Moreover, the NO fertigated seedlings maintained 57% higher chlorophyll contents than control seedlings. It is concluded that exogenous NO supply is an effective approach to ensure uniform stand establishment in saline regions of the world.

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