Effect of exogenous nitric oxide (NO) supply on germination and seedling growth of mungbean (cv. Nm-54) under salinity stress

2017 ◽  
Author(s):  
Muhammad Salahuddin ◽  
Fahim Nawaz ◽  
Muhammad Shahbaz ◽  
Muhammad Naeem ◽  
Bilal Zulfiqar ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Seedling Growth ◽  
Dry Matter ◽  
Germination Percentage ◽  
Stress Conditions ◽  
Tolerance Index ◽  
Fresh Weight ◽  
Chlorophyll Contents ◽  
Exogenous Nitric Oxide

The present study evaluated the effects of exogenous NO supply, using sodium nitroprusside (SNP) as a source of NO, on germination and seedling growth of mungbean (Vigna radiata) under salt stress conditions. The results showed that the seeds treated with NO solution (0.2 mM SNP) exhibited 80% and 109% higher germination percentage and germination stress tolerance index (GSI) than untreated seeds (control) under salt stress conditions. Similarly, the seedlings fertigated with NO maintained the highest values of 77.8%, 84.3%, 77.2%, 60.5% and 100.3% for plant height stress tolerance index (PHSI), root length stress tolerance index (RLSI), shoot fresh weight stress tolerance index, root fresh weight stress tolerance index (RFSI) and dry matter stress tolerance index (DMSI), respectively. Moreover, the NO fertigated seedlings maintained 57% higher chlorophyll contents than control seedlings. It is concluded that exogenous NO supply is an effective approach to ensure uniform stand establishment in saline regions of the world.

scholarly journals Role of L-Ornithine in Mitigation of Salt Stress In Allium Cepa L.

2021 ◽  
Vol 50 (4) ◽  
pp. 1165-1171
Author(s):  
Kürşat ÇAvuşoğlu ◽  
Di̇lek ÇAvuşoğlu
Keyword(s):  
Salt Stress ◽  
Mitotic Index ◽  
Seedling Growth ◽  
Chromosome Aberrations ◽  
Germination Percentage ◽  
Fresh Weight ◽  
Group Iii ◽  
Micronucleus Frequency ◽  
Group I ◽  
Allium Cepa L

Effects of L-ornithine (150 mg/l) on the germination, seedling growth, mitotic index, chromosome aberrations and micronucleus frequency of Allium cepa L. bulbs germinated at 0.125 M salinity were studied. The radicle number of the group III bulbs germinated in the medium with ornithine alone as compared to ones of the group I (control) bulbs which germinated in distilled water medium. But, their germination percentage, radicle length and fresh weight were statistically the same as ones of the group I bulbs. Besides, the micronucleus frequency and chromosomal abnormalities in the root-tip meristematic cells of the group III bulbs showed increased germination compared to ones of the group I bulbs. However, their mitotic index statistically showed the same value as the group I bulbs. Salt stress significantly inhibited the germination and seedling growth of A. cepa bulbs. Moreover, it reduced the mitotic index in the root-meristem cells of the bulbs and fairly increased the number of chromosome aberrations and micronucleus frequency. On the other hand, the inhibitive effect of salt on the germination, seedling growth, mitotic index and micronucleus frequency was dramatically alleviated in varying degrees by ornithine application. But, it was ineffective in reducing the detrimental effect of salinity on the chromosome aberrations. The germination percentage, radicle lenght, radicle number, fresh weight, mitotic index, micronucleus frequency and chromosomal aberrations of the group II seedlings grown in 0.125 M salinity were 27%, 13.5 mm, 18.4, 7.1 g, 5.5, 18.3 and 60.8%, respectively while these values became 68%, 16.4 mm, 16.4, 10.5 g, 15.6, 7.6 and 74.8% in the group IV seedlings treated with L-ornithine. Bangladesh J. Bot. 50(4): 1165-1171, 2021 (December)

scholarly journals Effects of NaCl on Germination and Seedling Growth in Macrotyloma uniflorum and Vigna mungo

2017 ◽  
Vol 5 (2) ◽  
pp. 169-176 ◽  
Author(s):  
SUMAN PANTOLA ◽  
KIRAN BARGALI ◽  
S.S. BARGALI ◽  
VIBHUTI VIBHUTI
Keyword(s):  
Salt Stress ◽  
Seedling Growth ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Vigna Mungo ◽  
Growth Stages ◽  
Tolerance Index ◽  
Abiotic Factor ◽  
Weight Percentage ◽  
Macrotyloma Uniflorum

Among abiotic stresses, drought and salinity are two major determinants due to high magnitude of their impact and wide occurrence. Salinity considerably limits the productivity of crops and thus, considered as the most destructive abiotic factor. In the present study, response of Macrotyloma uniflorum and Vigna mungo to salt (NaCl) stress imposed at germination and seedling growth stages was investigated. The aim of the study was identify the physiological and morphological responses of selected leguminous crop. Seeds were obtained from the healthy plants, surface sterilized and placed under six salt stress levels (0, 4, 8, 12, 16, 20 dsm-1). Complete randomized design with three replicates was used for this experiment and the experiments were conducted during the year 2015 in the glasshouse of Department of Botany, DSB, Campus, Kumaun University, Nainital. In comparison to Macrotyloma uniflorum (77%), higher germination percentage was observed in Vigna mungo (99%) at all salinity levels. In both the species, germination percentage and seedling growth decreased with the increase in salinity stress. Though Vigna mungo showed higher values for root (0.17 g) and shoot dry mass (0.27 g) the dry weight percentage reduction was higher in this species as compared to Macrotyloma uniflorum. Decrease in biomass of seedling with increasing salt stress indicated that the stress not only affected germination but also the growth of seedlings, which indicates that the synthetic ability of seed and biomass of the seedlings was also affected. Seed vigor index declined with the increase in salt concentrations. Outcomes from the study could be helpful in understanding the plant’s nature against different levels of salt stress and that could be economically exploited by various able agencies. At each salinity level, M. uniflorum showed higher salt tolerance index as compared to V. mungo so it can be cultivated in fields with salty soil.

scholarly journals Potasssium chloride as a nutrient seed primer to enhance salt‑tolerance in maize

2012 ◽  
Vol 47 (8) ◽  
pp. 1181-1184 ◽  
Author(s):  
Badar‑uz‑Zaman ◽  
Arshad Ali ◽  
Syed Ishtiaq Hyder ◽  
Muhammad Arshadullah ◽  
Saqib Umar Bhatti
Keyword(s):  
Salt Stress ◽  
Salinity Stress ◽  
Dry Matter ◽  
Germination Rate ◽  
Seed Priming ◽  
Germination Percentage ◽  
Stress Conditions ◽  
Maize Crop ◽  
Mean Germination Time ◽  
Muriate Of Potash

The objective of this work was to determine if KCl could be a useful nutrient primer for safe seed germination in maize crop under salt stress conditions. Seed priming was done using 50 mmol L‑1 of muriate of potash, and germination and seedling growth were evaluated after salt stress with NaCl up to 50 mmol L‑1. Another set of seeds was tested under the same salt stress conditions without priming. Under salinity stress, germination percentage, germination rate index, germination coefficient, and seedling vigor indexes were higher in primed seeds. In unprimed seeds, mean germination time increased, while the germination rate index and the fresh and dry matter mass decreased more sharply with salinity stress. The Na/K ratio was higher in unprimed seeds.

scholarly journals Effect of Seed Priming with Proline on Germination and Seedling Growth of Mungbean under Salt Stress

2019 ◽  
pp. 15-18
Author(s):  
Muhammad Abdus Sobahan
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Seedling Growth ◽  
Seed Priming ◽  
Germination Percentage ◽  
Fresh Weight ◽  
Seed Vigour ◽  
Control Seed ◽  
Vigour Index ◽  
Radicle Length

Seed priming is a pre-sowing treatment that enhances germination performance and stress tolerance of germinating seeds. The effect of seed priming with proline on germination and seedling growth of mungbean (Vigna radiata L.) under salt stress was investigated. The experiment carried out in completely randomized design with three replications in May 2018 at the Research Laboratory of the School of Agriculture and Rural Development, Bangladesh Open University, Gazipur, Bangladesh. Salt stress at 5 dSm-1 decreased seed germination percentage, plumule length, radicle length, plumule fresh weight, radicle fresh weight and seed vigour index compared to control. Seed priming with proline increased germination percentage (53.84%), plumule length, radicle length, plumule fresh weight, radicle fresh weight and seed vigour index under salt stress. The results suggested that seed priming with proline could effectively alleviate the inhibitory effects of salt stress on seed germination and seedling growth of mungbean. South Asian J. Agric., 7(1&2): 15-18

scholarly journals Study of Germination and Seedling Growth of Black Cumin (Nigella Sativa L.) Treated by Hydro and Osmopriming under Salt Stress Conditions

2015 ◽  
Vol 48 (2) ◽  
pp. 69-78 ◽  
Author(s):  
A. Ahmadian ◽  
Y. Shiri ◽  
M. Froozandeh
Keyword(s):  
Salt Stress ◽  
Water Potential ◽  
Seedling Growth ◽  
Nigella Sativa ◽  
Germination Percentage ◽  
Stress Conditions ◽  
Black Cumin ◽  
Osmotic Effect ◽  
Shoot Weight ◽  
Salt Toxicity

Abstract The objective of the study was to determine the responsible factors for germination and early seedling growth due to salt toxicity or osmotic effect and to optimize the best priming treatment for these stress conditions. To study the effect of osmopriming and hydropriming on germination and seedling growth of black cumin (Nigella sativa L.) under salt stress conditions this experiment was conducted at Torbat-Heydariyeh University, Torbat, Iran. The treated seeds (control, hydropriming and ZnSO4) of black cumin were evaluated at germination and seedling growth for tolerance to salt (NaCl and Na2SO4) conditions at the same water potentials of 0.0, -0.3, -0.6, -0.9 and -1.2MPa. Electrical conductivity (EC) values of the NaCl solutions were 0.0, 6.5, 12.7, 18.4 and 23.5 dSm-1, respectively. Results showed that hydropriming increased germination and seedling growth under salt stress. Germination delayed in both solutions, having variable germination with different priming treatments. In NaCl treatment, germination percentage, root and shoot weight, shoot and root length were higher but mean germination time and abnormal germination percentage were lower than Na2SO4, at the same water potential. The root / shoot weight and R/S length enhanced with increase of osmotic potential in both NaCl and Na2SO4 solutions. NaCl had less inhibitor effect on seedling growth than the germination. It was concluded that inhibition of germination at the same water potential of NaCl and Na2SO4 resulted from salt toxicity rather than osmotic effect. The findings of this experiment can be useful and applied to achieve best germination and uniform emergence under field conditions for farmers of medicinal plants.

Effects of Different Sr2+ Concentrations on Seedling Growth of Indica and Japonica Cultured by Hoagland Solution

2012 ◽  
Vol 524-527 ◽  
pp. 2097-2100 ◽  
Author(s):  
Xun Chao Xiang ◽  
Yan Fang Xu ◽  
Xiao Lin Long ◽  
Wen Li Su
Keyword(s):  
Enzyme Activity ◽  
Plant Growth ◽  
Stress Tolerance ◽  
Chlorophyll Content ◽  
Seedling Growth ◽  
Dry Weight ◽  
Fresh Weight ◽  
High Concentration ◽  
Hoagland Solution ◽  
Chlorophyll Contents

The paper studied capacity of Indica and Japonica in seedling for Sr2+ stress in different concentration. Seedlings of Indica and Japonica were cultured in 6 different gradient concentrations by using Hoagland solution. Fresh weight and dry weight, rice pigment content, enzyme activity for stress tolerance etc. were measured. The results showed that Sr2+ in low concentration could promote development and with high fresh/dry weight. At 10mmol/L of Sr2+ concentration, dry weight of Japonica Jing925 was increased by 27.40%, compared with control. However, Indica CG132R was only 7.8% over its control. Sr2+ of high concentration inhibited plant growth. They had higher chlorophyll content no matter Indica or Japonica when they were cultured in low Sr2+concentration. In contrast, their chlorophyll contents were decreased. Japonica had higher POD activity than Indica, and Indica had higher CAT activity than Japonica. All in all, Japonica Jing925 had stronger defense ability than Indica CG132R in adversity environment.

Salinity Tolerance of Black Gram Cultivars During Germination and Early Seedling Growth

2018 ◽  
Vol 51 (3) ◽  
pp. 51-68 ◽  
Author(s):  
M.K. Hasan ◽  
M.S. Islam ◽  
M.R. Islam ◽  
H.N. Ismaan ◽  
A. El Sabagh
Keyword(s):  
Salt Stress ◽  
Salinity Tolerance ◽  
Seedling Growth ◽  
Negative Impact ◽  
Germination Rate ◽  
Dry Weight ◽  
Germination Percentage ◽  
Shoot Length ◽  
Sand Culture ◽  
Black Gram

Abstract A laboratory experiment regarding germination and seedling growth test was conducted with three black gram genotypes tested under three salinity levels (0, 75 and 150 mM), for 10 days, in sand culture within small plastic pot, to investigate the germination and seedling growth characteristics. Different germination traits of all black gram genotypes, like germination percentage (GP), germination rate (GR), coefficient of velocity of germination (CVG) greatly reduced, as well as mean germination time (MGT) increased with increasing salt stress. At high salt stress, BARI Mash-3 provided the highest GP reduction (28.58%), while the lowest was recorded (15.79% to control) in BARI Mash-1. Salinity have the negative impact on shoot and root lengths, fresh and dry weights. The highest (50.32% to control) and lowest reduction (36.39%) of shoot length were recorded in BARI Mash-2 and BARI Mash-1, respectively, under 150 mM NaCl saline conditions. There were significant reduction of root lengths, root fresh and dry weight, shoot length, shoot fresh and dry weight in all genotypes under saline condition. The genotypes were arranged as BARI Mash-1 > BARI Mash-3 > BARI Mash-2, with respect to salinity tolerance.

Prioritisation of candidate genes in QTL regions for seed germination and early seedling growth in bread wheat (Triticum aestivum) under salt-stress conditions

2021 ◽  
Vol 72 (1) ◽  
pp. 1
Author(s):  
Elham Rezaei ◽  
Eslam Majidi Hervan ◽  
Amin Azadi ◽  
Alireza Etminan ◽  
Hossein Ramshini
Keyword(s):  
Triticum Aestivum ◽  
Salt Stress ◽  
Candidate Genes ◽  
Metabolic Process ◽  
Stress Conditions ◽  
Phenotypic Variance ◽  
Fresh Weight ◽  
Main Effect ◽  
Early Seedling ◽  
Go Terms

Salinity and drought are major abiotic stresses affecting wheat (Triticum aestivum L.) production throughout the world, and discovery of loci for traits affecting yield under salinity may lead to the breeding for salt-tolerant plants. In the present study, 186 F10 recombinant inbred line (RIL) populations were evaluated under salt-stress conditions in order to identify main-effect and epistatic-effect quantitative trait loci (QTLs) for 15 traits in wheat during the germination and early-seedling stages. In total, 61 main-effect QTLs on 15 chromosomes and 21 epistatic interactions on 12 chromosomes were detected through composite interval mapping (CIM) and a mixed-model-based CIM method. Two major QTLs for primary-leaf fresh weight and coleoptile fresh weight were detected on chromosome (or linkage group) 5B2 and 2D, respectively, which contributed ~44% and 43% of the phenotypic variance. Additionally, 12 QTL clusters including different traits were detected on 1A1, 3A, 4A, 2B1, 3B, 5B1 and 2D1. Candidate genes were identified within QTL regions and gene ontology (GO) enrichment analysis was performed. In total, 9134 candidate genes were grouped into 274 GO terms (including 79 GO terms involved in the ‘biological process’ category). These genes directly or indirectly play a vital role such as lipid localisation, biological regulation, fatty acid biosynthetic process, cellular process, DNA conformation change, translational elongation, carbohydrate metabolic process, Fe ion homeostasis, hydrogen peroxide metabolic process, and pigment biosynthetic process at the germination and early-seedling stages under salt-stress conditions.

Assessing allelopathic potential of Sonchus oleraceus L. (milk thistle) on germination and seedling growth of Oryza puncta

2021 ◽  
Vol 27 (1) ◽  
pp. 1-12
Author(s):  
Bilal Ahmad Khan ◽  
Saima Anwar ◽  
Rizwan Maqbool Maqbool ◽  
Muhammad Mohsin Amin ◽  
Muhammad Mansoor Javaid ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Seedling Growth ◽  
Dry Weight ◽  
Germination Percentage ◽  
Aqueous Extracts ◽  
Emergence Time ◽  
Fresh Weight ◽  
Water Control ◽  
Sonchus Oleraceus ◽  
Oryza Punctata

ABSTRACT Aqueous extracts of plants are used to control weeds and having no hazards to environment and are inexpensive. Present study was undertaken to investigate the effect of aqueous extracts of Sonchus oleraceus L. on the Oryza punctata L. emergence and initial seedling growth. It consists of aqueous extract of leaves, stem and fruit of S. oleraceus at varying concentrations 0.25, 0.50, 1, 2, 4 and 8 % (w/v) along with a distilled water control. The allelochemicals present in the aqueous extracts showed stimulatory, inhibitory and hormetic responses depending upon the concentration and the plant part. Results directed that maximum mean emergence time (5.26 days) and minimum germination index (1.67), germination percentage (40%), seedling fresh weight (59 g) and dry weight (8 g) of O. punctata were examined with 8% aqueous extract of S. oleraceus fruit. However, 4% aqueous extract of S. oleraceus fruit produced minimum root length (5.71). On the basis this experiment it was concluded that 8% aqueous extract of S. oleraceus fruit can be used for controlling O. punctata weed. Keywords: Aqueous extract, environment, allelochamicals, hermetic response, stimulatory ABSTRACT Aqueous extracts of plants are used to control weeds and having no hazards to environment and are inexpensive. Present study was undertaken to investigate the effect of aqueous extracts of Sonchus oleraceus L. on the Oryza punctata L. emergence and initial seedling growth. It consists of aqueous extract of leaves, stem and fruit of S. oleraceus at varying concentrations 0.25, 0.50, 1, 2, 4 and 8 % (w/v) along with a distilled water control. The allelochemicals present in the aqueous extracts showed stimulatory, inhibitory and hormetic responses depending upon the concentration and the plant part. Results directed that maximum mean emergence time (5.26 days) and minimum germination index (1.67), germination percentage (40%), seedling fresh weight (59 g) and dry weight (8 g) of O. punctata were examined with 8% aqueous extract of S. oleraceus fruit. However, 4% aqueous extract of S. oleraceus fruit produced minimum root length (5.71). On the basis this experiment it was concluded that 8% aqueous extract of S. oleraceus fruit can be used for controlling O. punctata weed. Keywords: Aqueous extract, environment, allelochamicals, hermetic response, stimulatory ABSTRACT Aqueous extracts of plants are used to control weeds and having no hazards to environment and are inexpensive. Present study was undertaken to investigate the effect of aqueous extracts of Sonchus oleraceus L. on the Oryza punctata L. emergence and initial seedling growth. It consists of aqueous extract of leaves, stem and fruit of S. oleraceus at varying concentrations 0.25, 0.50, 1, 2, 4 and 8 % (w/v) along with a distilled water control. The allelochemicals present in the aqueous extracts showed stimulatory, inhibitory and hormetic responses depending upon the concentration and the plant part. Results directed that maximum mean emergence time (5.26 days) and minimum germination index (1.67), germination percentage (40%), seedling fresh weight (59 g) and dry weight (8 g) of O. punctata were examined with 8% aqueous extract of S. oleraceus fruit. However, 4% aqueous extract of S. oleraceus fruit produced minimum root length (5.71). On the basis this experiment it was concluded that 8% aqueous extract of S. oleraceus fruit can be used for controlling O. punctata weed. Keywords: Aqueous extract, environment, allelochamicals, hermetic response, stimulatory

scholarly journals The Use of Germination Indices for Salinity Tolerance Classification of Pepper Cultivars

2019 ◽  
Vol 7 (9) ◽  
pp. 1304
Author(s):  
Gamze Kaya
Keyword(s):  
Salinity Tolerance ◽  
Seedling Growth ◽  
Growth Parameters ◽  
Genotypic Variation ◽  
Germination Percentage ◽  
Control Treatment ◽  
Tolerance Index ◽  
Seedling Length ◽  
Early Seedling ◽  
Germination Indices

The study aimed to evaluate the use of germination indices as a screening tool for salinity tolerance during germination and early seedling growth of pepper cultivars, and to distinguish the potential for genetic responses to salt tolerance. In the study, the seeds of seven pepper cultivars were germinated at increasing NaCl levels of 5, 10, 15 and 20 dS/m and distilled water as the control treatment for 14 days. Germination percentage (GP), mean germination time (MGT), germination index (GI), germination stress tolerance index (GSTI), seedling length (SL), seedling fresh weight (SFW) and vigor index (VI) were investigated. Results showed that germination percentage decreased with increasing NaCl levels while the highest germination percentage at 20 dS/m was 92% in BT Burdem with no significant reduction. Seedling growth of pepper cultivars was severely inhibited by increasing salinity stress. SFW was depressed depending on reduction in SL due to increasing NaCl. BT-Burli and BT İnce Sivri were the most tolerant cultivars to NaCl and they were used for genetic resources towards salinity. Seedling growth was much more sensitive to salinity than germination because of the highest percent reduction in seedling growth parameters. Among the parameters, GSTI gave the highest significant correlation coefficient with SL and SFW; indicating that it would be useful for estimating seedling growth. It was concluded that genotypic variation was observed among pepper cultivars for salinity tolerance and GSTI could be used for a predictor for salinity tolerance.

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