Evaluation of salt-tolerant germplasm and screening of the salt-tolerance traits of sweet sorghum in the germination stage

2018 ◽  
Vol 45 (10) ◽  
pp. 1073 ◽  
Author(s):  
Tonglou Ding ◽  
Zhen Yang ◽  
Xiaocen Wei ◽  
Fang Yuan ◽  
Shanshan Yin ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Sweet Sorghum ◽  
Development Process ◽  
Salt Tolerant ◽  
Comprehensive Index ◽  
Starting Point ◽  
High Salt Tolerance ◽  
Sorghum Bicolor L ◽  
Germination Stage ◽  
Selection Of

Soil salinity is one of the major abiotic stresses that reduces agricultural productivity and affects large terrestrial areas around the world. Germination is the starting point of the growth and development process of all crops, and it is severely affected by salt stress. Sweet sorghum (Sorghum bicolor (L.) Moench.) is one of the most promising crops that has a relatively high salt tolerance and biomass. However, few studies have evaluated the salt tolerance or screened the reliable evaluation traits of sweet sorghum. In this study, the membership function value of five traits was used as a comprehensive index for the evaluation and selection of salt tolerance in 300 sweet sorghum germplasms. After salt tolerance evaluation, 23 highly salt-tolerant, 38 salt-tolerant, 195 moderately salt-tolerant, 38 salt-sensitive and 6 highly salt-sensitive germplasms during the germination stage were screened. Moreover, the germination index under the 200 mM NaCl treatment showed the highest correlation with salt tolerance during the germination stage. This information can be used for effectively evaluating sweet sorghum during the germination stage. These results are important for the evaluation of the salt tolerance of sweet sorghum germplasms during the germination stage.

scholarly journals The full-length transcriptome of Spartina alterniflora reveals the complexity of high salt tolerance in monocotyledonous halophyte

2019 ◽  
Author(s):  
Wenbin Ye ◽  
Taotao Wang ◽  
Wei Wei ◽  
Shuaitong Lou ◽  
Faxiu Lan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Salt Stress ◽  
Alternative Splicing ◽  
Salt Tolerance ◽  
Protein Kinases ◽  
Spartina Alterniflora ◽  
High Salt ◽  
Full Length ◽  
Salt Tolerant ◽  
High Salt Tolerance

ABSTRACTSpartina alterniflora (Spartina) is the only halophyte in the salt marsh. However, the molecular basis of its high salt tolerance remains elusive. In this study, we used PacBio full-length single molecule long-read sequencing and RNA-seq to elucidate the transcriptome dynamics of high salt tolerance in Spartina by salt-gradient experiments (0, 350, 500 and 800 mM NaCl). We systematically analyzed the gene expression diversity and deciphered possible roles of ion transporters, protein kinases and photosynthesis in salt tolerance. Moreover, the co-expression network analysis revealed several hub genes in salt stress regulatory networks, including protein kinases such as SaOST1, SaCIPK10 and three SaLRRs. Furthermore, high salt stress affected the gene expression of photosynthesis through down-regulation at the transcription level and alternative splicing at the post-transcriptional level. In addition, overexpression of two Spartina salt-tolerant genes SaHSP70-I and SaAF2 in Arabidopsis significantly promoted the salt tolerance of transgenic lines. Finally, we built the SAPacBio website for visualizing the full-length transcriptome sequences, transcription factors, ncRNAs, salt-tolerant genes, and alternative splicing events in Spartina. Overall, this study sheds light on the high salt tolerance mechanisms of monocotyledonous-halophyte and demonstrates the potential of Spartina genes for engineering salt-tolerant plants.

scholarly journals Glassworts: From Wild Salt Marsh Species to Sustainable Edible Crops

Agriculture ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 14 ◽  
Author(s):  
Danilo Loconsole ◽  
Giuseppe Cristiano ◽  
Barbara De Lucia
Keyword(s):  
Salt Tolerance ◽  
Growth Media ◽  
Cultivated Land ◽  
Processed Food ◽  
Salt Tolerant ◽  
Factors Affecting ◽  
Edible Crops ◽  
High Salt Tolerance ◽  
Salt Marsh Species ◽  
Nutraceutical Properties

Halophytes are naturally adapted in saline environments, where they benefit from the substantial amounts of salt in the growth media. The need for salt-tolerant crops increases as substantial percentages of cultivated land worldwide are affected by salinity. There are few protocols, guidelines, or trials for glasswort (Salicornia (L.) and Sarcocornia (Scott), belong to the Amaranthaceae) field cultivation. The high salt tolerance and content in bioactive compounds make glassworts one of the most important candidates for future use both for fresh and processed food, due to their functional and health properties. This review describes the glassworts respect to their biodiversity and the most important factors affecting propagation, salt tolerance traits, agro-techniques and yields, food uses and nutraceutical properties.

scholarly journals Comprehensive Evaluation of Salt Tolerance in Asparagus Germplasm Accessions (Asparagus officinalis L.) at Different Growth Stages

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Huimin Gao ◽  
Xuhong Zhang ◽  
Yuqin Liang ◽  
Lingdi Dong ◽  
Changzhi Han ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Adult Stage ◽  
Seedling Stage ◽  
Asparagus Officinalis ◽  
Growth Stages ◽  
Salt Tolerant ◽  
Aboveground Part ◽  
The Mean ◽  
Germplasm Accessions ◽  
Germination Stage

The screening and cultivation of salt-tolerant crops are becoming more and more important owing to the constant increase in the saline soil area worldwide. Asparagus (A. officinalis L.) is a highly nutritious vegetable crop and widely consumed globally for a long time; however, little research has been done on asparagus. In this study, the salt tolerance of 95 asparagus germplasm accessions was evaluated at three growth stages (germination, seedling, and adult stages) under both salt-stressed and control conditions. Results showed that the growth parameters of most germplasm accessions were obviously inhibited by salt stress. The mean value of the seed germination rate at the germination stage decreased by half under salt-stressed conditions, the mean salt-injury index at the seedling stage reached 57.68%, and the fresh weight of the aboveground part (FWA) and the dry weight of the aboveground part (DWA) decreased the most among the traits determined at the adult stage by more than 60%. Our study screened out 30, 19, and 18 tolerant germplasm accessions (including highly salt-tolerant and salt-tolerant germplasm accessions) at the germination stage, seedling stage, and adult stage, respectively. Among them, two germplasm accessions (Ji08-2 and Jx1502) were simultaneously identified to be tolerant in all three growth stages, while other germplasm accessions were tolerant only at one or two stages. Thus, the salt tolerance of asparagus has periodic characteristics and changes throughout the lifecycle, and the identification of salt tolerance at all the main growth stages facilitates adequate assessment and application of tolerant germplasm accessions.

scholarly journals Comparative Proteomic Analysis of Tolerant and Sensitive Varieties Reveals That Phenylpropanoid Biosynthesis Contributes to Salt Tolerance in Mulberry

2021 ◽  
Vol 22 (17) ◽  
pp. 9402
Author(s):  
Tiantian Gan ◽  
Ziwei Lin ◽  
Lijun Bao ◽  
Tian Hui ◽  
Xiaopeng Cui ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Molecular Mechanism ◽  
Calcium Content ◽  
Heavy Metal Stress ◽  
Sodium Content ◽  
Proline Content ◽  
Salt Tolerant ◽  
Phenylpropanoid Biosynthesis ◽  
Selection Of

Mulberry, an important woody tree, has strong tolerance to environmental stresses, including salinity, drought, and heavy metal stress. However, the current research on mulberry resistance focuses mainly on the selection of resistant resources and the determination of physiological indicators. In order to clarify the molecular mechanism of salt tolerance in mulberry, the physiological changes and proteomic profiles were comprehensively analyzed in salt-tolerant (Jisang3) and salt-sensitive (Guisangyou12) mulberry varieties. After salt treatment, the malondialdehyde (MDA) content and proline content were significantly increased compared to control, and the MDA and proline content in G12 was significantly lower than in Jisang3 under salt stress. The calcium content was significantly reduced in the salt-sensitive mulberry varieties Guisangyou12 (G12), while sodium content was significantly increased in both mulberry varieties. Although the Jisang3 is salt-tolerant, salt stress caused more reductions of photosynthetic rate in Jisang3 than Guisangyou12. Using tandem mass tags (TMT)-based proteomics, the changes of mulberry proteome levels were analyzed in salt-tolerant and salt-sensitive mulberry varieties under salt stress. Combined with GO and KEGG databases, the differentially expressed proteins were significantly enriched in the GO terms of amino acid transport and metabolism and posttranslational modification, protein turnover up-classified in Guisangyou12 while down-classified in Jisang3. Through the comparison of proteomic level, we identified the phenylpropanoid biosynthesis may play an important role in salt tolerance of mulberry. We clarified the molecular mechanism of mulberry salt tolerance, which is of great significance for the selection of excellent candidate genes for saline-alkali soil management and mulberry stress resistance genetic engineering.

scholarly journals Large-scale integrated evaluation of salt tolerance in japonica rice at the germination stage

2020 ◽  
Author(s):  
Xiu Jing ◽  
Ping Mi ◽  
Xianzhi Xie ◽  
Baoshan Wang
Keyword(s):  
Mathematical Model ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Large Scale ◽  
Crop Yields ◽  
Japonica Rice ◽  
Rice Cultivars ◽  
Salt Tolerant ◽  
Fresh Weight ◽  
Germination Stage

Abstract Background: Salt stress, one of the most important abiotic stresses, severely reduces crop yields. Identifying salt-tolerant rice germplasm resources at the germination stage, developing salt tolerance indicators, and cultivating salt-tolerant rice cultivars are crucial for improving rice production in saline soil.Results: We measured the germination parameters of 140 japonica rice cultivars on the 7 day after sowing (DAS) in 0 and 150 mmol L−1 NaCl. To accurately assess salt tolerance and identify reliable indicators of salt tolerance, we measured the shoot length (SL), root length (RL), root fresh weight (RFW), shoot fresh weight (SFW), total fresh weight (TFW) and salt tolerance (STI) index after 7 days of salt-stress treatment. The 140 rice cultivars were divided into four categories based on the mean MFVs: highly salt tolerant (HST: 19 cultivars), salt tolerant (ST: 74 cultivars), weakly salt tolerant (WST: 43 cultivars), and salt sensitive (SS: 4 cultivars). Based on the physiological indicators, we established a mathematical model to accurately evaluate salt tolerance in japonica rice cultivars. STI of TFW under 150 mmol L−1 NaCl treatment showed the highest correlation with salt tolerance during the germination stage.Conclusions: We determined the optimum NaCl concentration (150 mmol L−1) for evaluating salt tolerance in japonica rice at the germination stage. We identified 19 HST, 74 ST, 43 WST, and 4 SS japonica rice cultivars during the germination stage and proposed a mathematical model to evaluate salt tolerance. STI of TFW is a reliable, accurate indicator for evaluating salt tolerance in japonica rice. These findings should greatly facilitate the evaluation of japonica rice cultivars during seed germination and the breeding of salt-tolerant rice cultivars.

scholarly journals Identification and Validation a Major QTL from “Sea Rice 86” Seedlings Conferred Salt Tolerance

Agronomy ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 410 ◽  
Author(s):  
Fengling Wu ◽  
Jun Yang ◽  
Diqiu Yu ◽  
Peng Xu
Keyword(s):  
Salt Tolerance ◽  
Oryza Sativa L ◽  
Bulked Segregant Analysis ◽  
Indica Rice ◽  
Chromosome 1 ◽  
Saline Stress ◽  
Salt Tolerant ◽  
Rice Varieties ◽  
Target Region ◽  
High Salt Tolerance

Saline stress severely affects rice (Oryza sativa L.) growth and development and reduces crop yield. Therefore, developing salt-tolerant and high-yielding rice using quantitative trait loci (QTLs) and linkage markers is a priority for molecular breeding. Here, the indica rice Sea Rice 86 (SR86) seedlings showed higher tolerance than ordinary rice varieties in saline soil, and a dominant effect on salinity sensitivity was demonstrated by genetic analysis. We constructed bulked segregant analysis pools using F2 populations from parents Dianjingyou 1 as the recipient and SR86 as the donor. We identified a 2.78 Mb region on chromosome 1 as the candidate region. Using simple sequence repeat markers and substitution analysis, we mapped the target region within 5.49 cM in the vicinity of markers RM8904–RM493. We speculated that this QTL, named qST1.1, might contribute significantly to the salt tolerance of SR86. The high salt tolerance of introgression lines obtained by marker assistant selection (MAS) confirmed that the qST1.1 region was associated with salinity tolerance. This newly-discovered QTL will be helpful for the analysis of the salt-tolerant mechanism of rice and breeding high-quality rice varieties using MAS.

scholarly journals Genome-wide association and differential expression analysis of salt tolerance in Gossypium hirsutum L at the germination stage

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Yanchao Yuan ◽  
Huixian Xing ◽  
Wenguan Zeng ◽  
Jialing Xu ◽  
Lili Mao ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Candidate Genes ◽  
Upland Cotton ◽  
Differentially Expressed ◽  
Genome Wide Association ◽  
Rna Seq ◽  
Salt Tolerant ◽  
Genome Wide ◽  
Germination Stage

Abstract Background Salinity is a major abiotic stress seriously hindering crop yield. Development and utilization of tolerant varieties is the most economical way to address soil salinity. Upland cotton is a major fiber crop and pioneer plant on saline soil and thus its genetic architecture underlying salt tolerance should be extensively explored. Results In this study, genome-wide association analysis and RNA sequencing were employed to detect salt-tolerant qualitative-trait loci (QTLs) and candidate genes in 196 upland cotton genotypes at the germination stage. Using comprehensive evaluation values of salt tolerance in four environments, we identified 33 significant single-nucleotide polymorphisms (SNPs), including 17 and 7 SNPs under at least two and four environments, respectively. The 17 stable SNPs were located within or near 98 candidate genes in 13 QTLs, including 35 genes that were functionally annotated to be involved in salt stress responses. RNA-seq analysis indicated that among the 98 candidate genes, 13 were stably differentially expressed. Furthermore, 12 of the 13 candidate genes were verified by qRT-PCR. RNA-seq analysis detected 6640, 3878, and 6462 differentially expressed genes at three sampling time points, of which 869 were shared. Conclusions These results, including the elite cotton accessions with accurate salt tolerance evaluation, the significant SNP markers, the candidate genes, and the salt-tolerant pathways, could improve our understanding of the molecular regulatory mechanisms under salt stress tolerance and genetic manipulation for cotton improvement.

scholarly journals High and differential strontium tolerance in germinating dimorphic seeds of Salicornia europaea

2020 ◽  
Vol 48 (2) ◽  
pp. 231-239 ◽  
Author(s):  
Li Jiang ◽  
Mohsin Tanveer ◽  
Wei Han ◽  
Changyan Tian ◽  
Lei Wang
Keyword(s):  
Salt Tolerance ◽  
Plant Stress ◽  
Critical Value ◽  
Germination Percentage ◽  
Salt Tolerant ◽  
Salicornia Europaea ◽  
Polluted Soils ◽  
High Salt Tolerance ◽  
Plant Stress Tolerance ◽  
High Concentrations

Salicornia europaea, a highly salt-tolerant halophyte, is potentially resistant to other metals because plant stress tolerance partly relies on common physiochemical mechanisms. Large median seeds and small lateral seeds of S. europaea have high salt tolerance and display contrasting germination responses. Thus, we hypothesised that dimorphic seeds of S. europaea might also have high and differential strontium (Sr) tolerance during germination. Both types of seeds were incubated in different SrCl2 concentrations at 25°C. 0-300 mmol L-1 SrCl2 did not significantly affect germination of median seeds. However, for lateral seeds, relatively high concentrations (≥ 200 mmol L-1) dramatically inhibited germination. The simulated critical value (when germination percentage is 50%) was 502 mmol L-1 for median seeds and 224 mmol L-1 for lateral seeds. Dimorphic seeds of S. europaea are highly tolerant to Sr stress and large median seeds display higher tolerance. The results suggest that direct seeding of large seeds of S. europaea might be an effective method to remediate heavy Sr-polluted soils.

Salt tolerance of cowpea genotypes in the emergence stage

2001 ◽  
Vol 41 (1) ◽  
pp. 81 ◽  
Author(s):  
B. Murillo-Amador ◽  
E. Troyo-Diéguez ◽  
A. López-Cortés ◽  
H. G. Jones ◽  
F. Ayala-Chairez ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Vigna Unguiculata ◽  
Salt Tolerant ◽  
Root:Shoot Ratio ◽  
Salinity Effects ◽  
Class A ◽  
Class B ◽  
Early Seedling ◽  
Emergence Percentage ◽  
Germination Stage

The effect of NaCl salinity (0, 85 and 170 mmol/L) during emergence of 25 genotypes of cowpea [Vigna unguiculata (L.) Walp.] was studied under growth chamber conditions. Seed emergence percentage and rate, root:shoot ratio, and biomass per plant were affected by genotype, salinity and genotype salinity interaction; this interaction showed that salinity effects differed among genotypes. The criterion used to classify genotypes with respect to their salt tolerance was based on their germination percentages in both 85 and 170 mmol NaCl/L. One genotype was grouped into class ‘A’ (CB27) which had the highest salt tolerance at emergence, and was classified as salt tolerant. Another group consisting of Paceño, CB88, CB3, CB5, Tardón, Cuarenteño and CB46 was placed into class ‘B’; these genotypes showed total emergence percentages up to 75% in both 85 and 170 mmol NaCl/L. A third group of genotypes was placed into class ‘C’ which had the lowest emergence percentages in both 85 and 170 mmol NaCl/L. It was confirmed that salinity treatments affect the emergence of cowpea, delaying both emergence percentage and rate. We conclude that selection and classification for salt tolerance in cowpea genotypes can be successfully undertaken at early seedling stages, because the same genotypes were classified similarly during the germination stage in previous research.

scholarly journals Screening of Purslane (Portulaca oleraceaL.) Accessions for High Salt Tolerance

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Md. Amirul Alam ◽  
Abdul Shukor Juraimi ◽  
M. Y. Rafii ◽  
Azizah Abdul Hamid ◽  
Farzad Aslani
Keyword(s):  
Salt Tolerance ◽  
Salinity Stress ◽  
Dry Matter ◽  
Population Pressure ◽  
Yield Reduction ◽  
Salt Tolerant ◽  
Salinity Levels ◽  
Cultivable Land ◽  
High Salt Tolerance ◽  
Crop Variety

Purslane (Portulaca oleraceaL.) is an herbaceous leafy vegetable crop, comparatively more salt-tolerant than any other vegetables with high antioxidants, minerals, and vitamins. Salt-tolerant crop variety development is of importance due to inadequate cultivable land and escalating salinity together with population pressure. In this view a total of 25 purslane accessions were initially selected from 45 collected purslane accessions based on better growth performance and subjected to 5 different salinity levels, that is, 0.0, 10.0, 20.0, 30.0, and 40.0 dS m−1NaCl. Plant height, number of leaves, number of flowers, and dry matter contents in salt treated purslane accessions were significantly reduced (P≤0.05) and the enormity of reduction increased with increasing salinity stress. Based on dry matter yield reduction, among all 25 purslane accessions 2 accessions were graded as tolerant (Ac7 and Ac9), 6 accessions were moderately tolerant (Ac3, Ac5, Ac6, Ac10, Ac11, and Ac12), 5 accessions were moderately susceptible (Ac1, Ac2, Ac4, Ac8, and Ac13), and the remaining 12 accessions were susceptible to salinity stress and discarded from further study. The selected 13 purslane accessions could assist in the identification of superior genes for salt tolerance in purslane for improving its productivity and sustainable agricultural production.

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