101 Effects of Active Immunization Against GnRH in Oocyte Donors with Cystic Ovarian Disease

2018 ◽  
Vol 30 (1) ◽  
pp. 190
Author(s):  
O. A. C. Faria ◽  
L. R. O. Dias ◽  
L. Leme ◽  
G. Fernandes ◽  
A. A. G. Fidelis ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Endometrial Thickness ◽  
Active Immunization ◽  
Response To Treatment ◽  
Control Group ◽  
Individual Response ◽  
Embryo Production ◽  
Ovarian Disease ◽  
Oocyte Donors ◽  
Cystic Ovarian Disease

Cows intensively used as oocyte donors for in vitro embryo production (IVEP) are usually kept nonpregnant for prolonged intervals, exposed to successive hormonal treatments, and frequently become overweight. These are all risk factors for the development of endocrine unbalance and, consequently, cystic ovarian disease (COD). The aim of this study was to evaluate the effect of active immunization against gonadotropin-releasing hormone (GnRH) on (1) ovarian follicular population, and (2) development potential of oocytes used for IVEP. Nelore (Bos indicus) cows (n = 14), previously diagnosed with chronic COD (Faria et al. 2017 Anim. Reprod., in press), weighing 620.0 ± 12.8 kg and with body condition score of 4.1 ± 0.2, were assigned to control (n = 6) or treatment (n = 8) group. Cows in the treatment group received 2 SC injections of 1.0 mL of anti-GnRH vaccine (Bopriva, Zoetis, Brazil), 28 days apart (weeks 0 and 4), whereas cows in the control group received placebo on the same schedule. Transrectal ultrasonography was performed weekly from week 0 to evaluate the number and distribution of follicles among size classes, endometrial thickness, and clinical presence of mucometra. Immunization was considered effective (E-IM) when no follicles ≥5.0 mm were observed on the ovaries during a given examination. Cows having E-IM were then used as oocyte donors for IVEP. Cumulus-oocyte complexes (COC) were collected in 5 consecutive ovum pick-up weekly sessions. As a control for IVEP, oocytes from a slaughterhouse were used, with similar procedures performed on the same days and using the same semen batch. The MIXED procedure of SAS (SAS Institute Inc., Cary, NC, USA) with repeated-measures statement was used to evaluate the effects of treatment, time, and interactions on ovarian endpoints; and the GLM procedure was used to analyse embryo production data. Results are shown as mean ± SEM. There were time and time × treatment effects on ovarian parameters. Treated cows had a decrease (P < 0.05) in the average diameter of the largest follicle and in the number of follicles ≥8 mm, and an increase (P < 0.05) in follicular population after week 6. Nonetheless, individual response to treatment was variable: only 50% of the cows (4 of 8) were E-IM at week 8, whereas 25% (2 of 8) still had COD (largest follicle ≥18.0 mm) at this timepoint. Overall, a negative correlation was detected between follicular population and the diameter of the largest follicle (r = –0.60, P < 0.0001) or the number of follicles ≥8 mm (r = –0.47, P < 0.0001). There was no effect (P > 0.05) of treatment on endometrial thickness or mucometra score. Cows with E-IM produced 22.2 ± 3.6 total and 12.9 ± 2.3 viable COC. Cleavage rate did not differ between E-IM and control (slaughterhouse) oocytes (70.8 ± 7.0 v. 75.1 ± 3.0%, respectively; P > 0.05); however, blastocyst rate was greater in the E-IM group compared with controls (39.7 ± 5.5 v. 20.5 ± 4.7%, respectively; P < 0.02). In summary, our results suggest that active immunization against GnRH leads to variable results in the distribution of the follicular population in cows with COD, but it does not negatively affect IVEP efficiency. This research was supported by Zoetis, CNPq, and CAPES.

Active immunization against GnRH as an alternative therapeutic approach for the management of Bos indicus oocyte donors diagnosed with chronic cystic ovarian disease

2021 ◽  
Author(s):  
Joao Henrique Moreira Viana ◽  
Nathalia Ellen Sousa Pereira ◽  
Otavio Augusto Costa Faria ◽  
Luzia Renata Oliveira Dias ◽  
Eduardo Ramos Oliveira ◽  
...  
Keyword(s):  
Therapeutic Approach ◽  
Bos Indicus ◽  
Active Immunization ◽  
Ovarian Disease ◽  
Alternative Therapeutic Approach ◽  
Oocyte Donors ◽  
Cystic Ovarian Disease

scholarly journals The intra-articular administration of triamcinolone hexacetonide in the treatment of osteoarthritis. Its effects in a naturally occurring canine osteoarthritis model

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0245553
Author(s):  
João C. Alves ◽  
Ana Santos ◽  
Patrícia Jorge ◽  
Catarina Lavrador ◽  
L. Miguel Carreira
Keyword(s):  
Weight Distribution ◽  
Repeated Measures ◽  
Weight Bearing ◽  
Interleukin 1 ◽  
Response To Treatment ◽  
Control Group ◽  
Radiographic Findings ◽  
Triamcinolone Hexacetonide ◽  
Paired Samples ◽  
Kaplan Meier

Objective To evaluate the effect of an intra-articular (IA) administration of triamcinolone hexacetonide, compared with saline. Patients and methods Forty (N = 40) hip joints were randomly assigned to a treatment group (THG, n = 20, receiving IA triamcinolone hexacetonide) and a control group (CG, n = 20, receiving IA saline). On treatment day (T0), and at 8, 15, 30, 90 and 180 days post-treatment, weight distribution, joint range of motion, thigh girth, digital thermography, radiographic signs, synovial fluid interleukin-1 and C-reactive protein levels were evaluated. Data from four Clinical Metrology Instruments was also gathered. Results were compared Repeated Measures ANOVA, with a Huynh-Feldt correction, Paired Samples T-Test or Wilcoxon Signed Ranks Test. A Kaplan-Meier test was performed to compare both groups, with p<0.05. Results Joints were graded as mild (65%), moderate (20%) and severe (15%). Patients of both sexes, with a mean age of 6.5±2.4 years and bodyweight of 26.7±5.2kg, were included. No differences were found between groups at T0. Comparing THG to CG, weight distribution showed significant improvements in THG from 8 (p = 0.05) up to 90 days (p = 0.01). THG showed lower values during thermographic evaluation in the Lt view (p<0.01). Pain and function scores also improved from 30 to 180 days. Increasing body weight, age, and presence of caudolateral curvilinear osteophyte corresponded to worse response to treatment. Results of the Kaplan Meier test showed significant differences between groups, with THG performing better considering several evaluations and scores. Conclusion THG recorded significant improvements in weight-bearing and in with the considered CMIs, particularly pain scores. Lower thermographic values were registered in THG up to the last evaluation day. Age, sex, and radiographic findings did significantly influenced response to treatment.

Response Variability and Detraining Effects of Standardized Exercise Programs

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Martín–Guillaumes J ◽  
◽  
Montull L ◽  
Ventura JL ◽  
Javierre C ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Training Programs ◽  
Maximal Oxygen Consumption ◽  
Control Program ◽  
Training Group ◽  
Response Variability ◽  
Male Students ◽  
Control Group ◽  
Time To Exhaustion ◽  
Individual Response

Purpose: To compare inter–individual response variability and detraining effects on markers attributed to aerobic and anaerobic performance after shortterm standardized aerobic, strength and mixed training programs. Methods: Thirty–six male students were randomly assigned to either an aerobic, strength, mixed, or control program (9 per group). They performed two consecutive cycling tests (incremental and plateau) to exhaustion at three points: 1 week before training, after 6 weeks of training, and 3 weeks after the training was finished. Maximal oxygen consumption (VO2max), maximal workload (Wmax), and time to exhaustion performed at Wmax (W × time) were compared between groups by repeated–measures ANOVA with Bonferroni post–hoc tests. The inter–subject response variability within each training group was evaluated by comparison with the 95% confidence interval of the control group. Detraining effects were evaluated using the hysteresis areas, which were compared between each training group and the control group by Mann–Whitney U test. Results: Differences were observed in Wmax for the aerobic (F(2,7)=19.562; p=0.001; n²=0.85) and mixed (F(2,7)=13.447; p=0.004; n²=0.99) programs, and in W × time for the mixed program (F(2,7)=15.432; p= 0.016; n²=0.89). There was high inter–subject response variability for all variables and training programs, except for a homogenous positive response to Wmax in the mixed program (X²=6.27; p=0.04). Detraining effects of Wmax were also better maintained after the mixed program. Conclusion: A mixed program of aerobic and strength training demonstrated higher improvements in the studied markers of performance, with lower interindividual response variability, and longer detraining effects compared with aerobic or strength programs.

scholarly journals BMP2, 4 and 6 and BMPR1B are altered from early stages of bovine cystic ovarian disease development

Reproduction ◽  
2016 ◽  
Vol 152 (4) ◽  
pp. 333-350 ◽  
Author(s):  
Pablo U Díaz ◽  
Gustavo J Hein ◽  
Eduardo M Belotti ◽  
Fernanda M Rodríguez ◽  
Florencia Rey ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Bone Morphogenetic Proteins ◽  
Control Group ◽  
Disease Development ◽  
Low Doses ◽  
Spanish Translation ◽  
Antral Follicles ◽  
Ovarian Disease ◽  
Theca Cells ◽  
Cystic Ovarian Disease

Cystic ovarian disease (COD) is an important cause of subfertility in dairy cattle. Bone morphogenetic proteins (BMPs), mainly BMP2, BMP4 and BMP6, play a key role in female fertility. In this study, we hypothesized that an altered BMP system is associated with ovarian alterations contributing to COD pathogenesis. Therefore, we examined the expression of BMP2, BMP4 and BMP6 and BMP receptor 1B (BMPR1B) in the ovaries of animals with spontaneous or ACTH-induced COD, as well as during the development of the disease, in a model of follicular persistence induced by low doses of progesterone (at 5, 10 and 15 days of follicular persistence). Results showed changes in BMP2, BMP4 and BMP6 expression during folliculogenesis, in granulosa and theca cells in the COD groups, as well as at different stages of follicular persistence. Results also showed changes in BMPR1B expression in developing follicles in animals with COD, and at the initial stages of follicular persistence (P5). Comparison between groups showed significant differences, mainly in BMP4 and BMP6 expression, in granulosa and theca cells of different follicular categories. The expression of these BMPs also increased in cystic and persistent follicles, in relation to antral follicles of the control group. BMPR1B showed high expression in cystic follicles. Together, these results may indicate an alteration in BMPs, especially in BMP4 and BMP6, as well as in BMPR1B, which occurs early in folliculogenesis and incipiently during the development of COD, which could be a major cause of recurrence of this disease in cattle. Free Spanish abstract: A Spanish translation of this abstract is freely available at http://www.reproduction-online.org/content/152/4/333.abstract.

scholarly journals Research of postpartum endometritis in Japanese Black cattle with cystic ovarian disease by vaginal mucus test and endometrial cytology

2020 ◽  
Vol 63 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Naoki Yamamoto ◽  
Ryo Nishimura ◽  
Yosuke Gunji ◽  
Mitsugu Hishinuma
Keyword(s):  
Endometrial Thickness ◽  
Polymorphonuclear Neutrophils ◽  
Ovarian Activity ◽  
Japanese Black Cattle ◽  
Specific Symptom ◽  
Potential Implication ◽  
Ovarian Disease ◽  
The Mean ◽  
Cystic Ovarian Disease ◽  
Endometrial Cytology

Abstract. The relationship between endometritis and cystic ovarian disease (COD) is still unclear in Japanese Black cattle. Endometritis is classified into clinical endometritis (CE) and subclinical endometritis (SE). The objective of this study was to clarify the interaction between postpartum endometritis (CE and SE) and COD in Japanese Black cattle. Twenty-six suckled cows with COD (COD group) and 16 suckled cows with cyclical ovarian activity (CA group) were submitted for the experiment. Uterine conditions of cows were classified into three groups (normal, CE, and SE) with vaginal mucus test and endometrial cytology. The combined data of CE and SE were represented as data for total endometritis (EMT total). The prevalence of EMT total in the COD group (42.3 %, 11∕26) was significantly higher than that of the CA group (12.5 %, 2∕16). The mean percentage of polymorphonuclear neutrophils (PMN %) in the COD group was significantly higher than that of the CA group at 40–60 DPP (days postpartum). Compared to 61–295 DPP, the mean PMN % at 40–60 DPP was significantly higher in the COD group. The diameters of uterine horn and cervix did not differ among normal uterine condition, CE and SE in the COD group, and they did not differ between normal uterine condition and SE in the CA group. However, endometrial thickness during both 40–60 and 61–295 DPP were greater in the COD group than in the CA group. In conclusion, Japanese Black cattle with COD have a potential implication on endometritis at 40–60 DPP compared to the normal ovarian cycle. As a specific symptom was not observed by transrectal ultrasonography, endometrial cytology is effective for diagnosis of SE in Japanese Black cattle.

4 Induction of ovulation by kisspeptin in llamas

2019 ◽  
Vol 31 (1) ◽  
pp. 127 ◽  
Author(s):  
R. A. Carrasco ◽  
C. E. Leonardi ◽  
K. D. Hutt ◽  
J. Singh ◽  
G. P. Adams
Keyword(s):  
Body Weight ◽  
Corpus Luteum ◽  
Repeated Measures ◽  
Ovarian Function ◽  
Gnrh Receptor ◽  
Response To Treatment ◽  
Transrectal Ultrasonography ◽  
Control Group ◽  
Gnrh Analogue ◽  
Dominant Follicle

After mating, female camelids ovulate in response to nerve growth factor (NGF) present in semen (formerly referred to as ovulation-inducing factor). The ovulatory effect appears to be induced by stimulation of hypothalamic gonadotropin-releasing hormone (GnRH) neuronal activity and LH secretion. Recent studies have identified kisspeptin as an important mediator of GnRH secretion in several species. In the present study, we tested the hypothesis that kisspeptin is involved in the ovulatory pathway in llamas and investigated the mechanism of this effect. In Experiment 1, ovarian function in non-pregnant, non-lactating adult female llamas was synchronized by intramuscular administration of a GnRH analogue (50µg of gonadorelin acetate; Fertiline, Vetoquinol, Quebec, QC, Canada). When a growing dominant follicle ≥8mm in diameter was detected, llamas were assigned randomly to 3 treatment groups and given an intravenous dose of purified seminal NGF (1mg, single dose; n=5), kisspeptin (0.1mg kg−1 of body weight, 2 doses 1h apart; n=5), or PBS (n=4). The bioactive 10 amino acid fragment of murine kisspeptin was used. Ovulation and corpus luteum development were assessed by transrectal ultrasonography every other day from the day of treatment (Day 0) to Day 8. In Experiment 2, ovarian function among female llamas was synchronized, as in Experiment 1. When a growing dominant follicle ≥8mm in diameter was detected, llamas were given kisspeptin (0.1mg kg−1 of body weight IV, 2 doses 1h apart) beginning 2h after pretreatment with either a GnRH receptor blocker (cetrorelix acetate, 1.5mg per llama IV; Sigma, Oakville, ON, Canada; n=6) or saline (n=6). Llamas were examined 48h later by transrectal ultrasonography to detect ovulation and 8 days later to determine the presence of a corpus luteum. Chi-square tests were used to compare ovulation rates, and ANOVA for repeated measures was used to compare diameter profiles of the corpus luteum. In Experiment 1, ovulation rate did not differ between the NGF and kisspeptin groups (5/5 in each; 100%) and was greater than in the control group (0/4; 0% P&lt;0.05). Corpus luteum diameter did not differ between llamas that ovulated in response to treatment with NGF or kisspeptin (13.2±0.8 and 14.0±1.2mm on Day 8, respectively). In Experiment 2, none of the llamas pretreated with cetrorelix ovulated in response to kisspeptin treatment (0/6; 0%), whereas all of the llamas pretreated with saline ovulated in response to kisspeptin treatment and had a corpus luteum at Day 8 (6/6; 100%; P&lt;0.05). Results supported the hypothesis that kisspeptin induces ovulation in llamas. Because a GnRH receptor antagonist blocked ovulation, our interpretation is that the site of action of kisspeptin is upstream of the pituitary gland and involves control of GnRH release from the hypothalamus. These findings raise the possibility that kisspeptin mediates the ovulation-inducing effect of NGF. Research was supported by the Natural Sciences and Engineering Research Council of Canada.

scholarly journals Effect of a single intra-articular high molecular weight hyaluronan in a naturally occurring canine osteoarthritis model: a randomized controlled trial

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
J. C. Alves ◽  
Ana Margarida Moniz Pereira dos Santos ◽  
Patrícia Jorge ◽  
Catarina Falcão Trigoso Vieira Bran Lavrador ◽  
L. Miguel Carreira
Keyword(s):  
Repeated Measures ◽  
Financial Burden ◽  
Controlled Trial ◽  
Interleukin 1 ◽  
Well Being ◽  
Joint Disease ◽  
Response To Treatment ◽  
Control Group ◽  
Paired Samples ◽  
Treatment Experiment

Abstract Background Osteoarthritis (OA) is a complex joint disease and chronic pain source, affecting a patient’s quality of life and posing a financial burden. As the dog is considered a nearly ideal species for translation research of human OA and the most used model for research, exploring spontaneous dog OA under the One Health/One Medicine concept can improve both humans and dogs’ health and well-being. Methods In a clinical treatment experiment, forty (N=40) joints were selected and randomly assigned to a control group (CG), which received 0.9% NaCl or a treatment (HG), which received Hylan G-F 20. Evaluations were performed on treatment day (T0), 8, 15, 30, 90, and 180 days post-treatment. They consisted of four different Clinical Metrology Instruments (CMI), evaluation of weight distribution, joint range of motion, thigh girth, radiographic and digital thermography imaging, synovial fluid interleukin-1 (IL-1), and C-reactive protein concentrations. Results were compared with repeated measures ANOVA, with a Huynh-Feldt correction, Paired samples T-test, or Wilcoxon signed-ranks test, with p<0.05. Results Patients had a mean age of 6.5±2.4 years and a bodyweight of 26.6±5.2kg, and joints graded as mild (n=28, 70%), moderate (n=6, 15%), and severe OA (n=6, 15%). No differences were found between groups at T0. Symmetry index and deviation showed significant improvements in HG from 30 days (p<0.01) up to 180 days (p=0.01). Several CMI scores, particularly pain scores, improved from 90 to 180 days. Radiographic signs progressed in both groups. In both groups, increasing body weight and age corresponded to worse clinical presentation. IA hyaluronan administration produced increased lameness in six cases, which resolved spontaneously. Conclusions This study characterizes the response to treatment with Hylan G-F 20, which can produce significant functional and pain level improvements in patients with OA, even those with factors related to worse response to treatment.

Intracisternal BioGlue injection in the fetal lamb: a novel model for creation of obstructive congenital hydrocephalus without additional chemically induced neuroinflammation

2019 ◽  
Vol 24 (6) ◽  
pp. 652-662 ◽  
Author(s):  
Marc Oria ◽  
Soner Duru ◽  
Federico Scorletti ◽  
Fernando Vuletin ◽  
Jose L. Encinas ◽  
...  
Keyword(s):  
Calcium Binding ◽  
Repeated Measures ◽  
Obstructive Hydrocephalus ◽  
Critical Threshold ◽  
Control Group ◽  
Chronic Obstructive ◽  
Fetal Sheep ◽  
Cisterna Magna ◽  
Lateral Ventricles ◽  
Fetal Lamb

OBJECTIVEThe authors hypothesized that new agents such as BioGlue would be as efficacious as kaolin in the induction of hydrocephalus in fetal sheep.METHODSThis study was performed in 34 fetal lambs randomly divided into 2 studies. In the first study, fetuses received kaolin, BioGlue (2.0 mL), or Onyx injected into the cisterna magna, or no injection (control group) between E85 and E90. In the second study, fetuses received 2.0-mL or 2.5-mL injections of BioGlue into the cisterna magna between E85 and E90. Fetuses were monitored using ultrasound to assess lateral ventricle size and progression of hydrocephalus. The fetuses were delivered (E120–E125) and euthanized for histological analysis. Selected brain sections were stained for ionized calcium binding adaptor 1 (Iba1) and glial fibrillary acidic protein (GFAP) to assess the presence and activation of microglia and astroglia, respectively. Statistical comparisons were performed with Student’s t-test for 2 determinations and ANOVA 1-way and 2-way repeated measures for multiple determinations.RESULTSAt 30 days after injection, the lateral ventricles were larger in all 3 groups that had undergone injection than in controls (mean diameter in controls 3.76 ± 0.05 mm, n = 5). However, dilatation was greater in the fetuses injected with 2 mL of BioGlue (11.34 ± 4.76 mm, n = 11) than in those injected with kaolin (6.4 ± 0.98 mm, n = 7) or Onyx (5.7 ± 0.31 mm, n = 6) (ANOVA, *p ≤ 0.0001). Fetuses injected with 2.0 mL or 2.5 mL of BioGlue showed the same ventricle dilatation but it appeared earlier (at 10 days postinjection) in those injected with 2.5 mL. The critical threshold of ventricle dilatation was 0.1 for all the groups, and only the BioGlue 2.0 mL and BioGlue 2.5 mL groups exceeded this critical value (at 30 days and 18 days after injection, respectively) (ANOVA, *p ≤ 0.0001). Moderate to severe hydrocephalus with corpus callosum disruption was observed in all experimental groups. All experimental groups showed ventriculomegaly with significant microgliosis and astrogliosis in the subventricular zone around the lateral ventricles. Only kaolin resulted in significant microgliosis in the fourth ventricle area (ANOVA, *p ≤ 0.005).CONCLUSIONSThe results of these studies demonstrate that BioGlue is more effective than Onyx or kaolin for inducing hydrocephalus in the fetal lamb and results in a volume-related response by obstructive space-occupancy without local neuroinflammatory reaction. This novel use of BioGlue generates a model with potential for new insights into hydrocephalus pathology and the development of therapeutics in obstructive hydrocephalus. In addition, this model allows for the study of acute and chronic obstructive hydrocephalus by using different BioGlue volumes for intracisternal injection.

scholarly journals Effectiveness of Abdominal and Gluteus Medius Training in Lumbo-Pelvic Stability and Adductor Strength in Female Soccer Players. A Randomized Controlled Study

2021 ◽  
Vol 18 (4) ◽  
pp. 1528
Author(s):  
Héctor Guerrero-Tapia ◽  
Rodrigo Martín-Baeza ◽  
Rubén Cuesta-Barriuso
Keyword(s):  
Repeated Measures ◽  
Gluteus Medius ◽  
Soccer Players ◽  
Controlled Study ◽  
Control Group ◽  
Extrinsic Factors ◽  
Randomized Controlled ◽  
Limb Injuries ◽  
Experimental Group

Background. Abdominal and lumbo-pelvic stability alterations may be the origin of lower limb injuries, such as adductor pathology in soccer players. Imbalance can be caused by both intrinsic and extrinsic factors. Methods: In this randomized controlled trial over 8 weeks, 25 female footballers were randomly allocated to an experimental group (isometric abdominal training and gluteus medius-specific training) or a control group (isometric abdominal training). Evaluations were performed at baseline, at the end of the intervention and after a 4-week follow-up period. The exercise protocol in common for both groups included three exercises: Plank, Lateral plank and Bird dog. Specific exercises for the gluteus medius were: Pelvic drop and Stabilization of the gluteus medius in knee valgus. Outcome measures were lumbar-pelvic stability and adductor strength. Results: After the intervention, there was an increase in lumbo-pelvic stability in both groups, being greater in the control group than in the experimental group (mean differences [MD]: 4.84 vs. MD: 9.58; p < 0.01) with differences in the analysis of repeated measures (p < 0.001), but not in group interaction (p = 0.26). Changes were found in adductor strength in the experimental group (MD: −2.48; p < 0.001 in the left adductor; MD: −1.48; p < 0.01 in right adductor) and control group (MD: −1.68; p < 0.001 in the left adductor; MD: −2.05; p < 0.001 in the right adductor) after the intervention, with differences in the analysis of repeated measures in left (p < 0.001) and right (p < 0.001) adductor strength. Conclusions: An abdominal and gluteal training protocol shows no advantage over a protocol of abdominal training alone for lumbo-pelvic stability and adductor strength, while improvements in both variables are maintained at four weeks follow-up.

scholarly journals Cytotoxicity assessment of different doses of ozonated water on dental pulp cells

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ferdiye Küçük ◽  
Sibel Yıldırım ◽  
Serap Çetiner
Keyword(s):  
Cell Viability ◽  
Repeated Measures ◽  
Dental Pulp ◽  
Control Group ◽  
Dental Pulp Cells ◽  
Time Points ◽  
Significant Difference ◽  
Pulp Cells ◽  
Time Point ◽  
Ozonated Water

Abstract Background The purpose of this study was to assess the cytotoxicity of various concentrations of ozonated water (OW) on human primary dental pulp cells. Methods Human primary dental pulp cells were isolated from exfoliated primary canine teeth of an 11-year-old patient with good systemic and oral health. Afterwards, cells were divided into 6 experimental groups; four groups of OW in concentrations of 2 mg/L, 4 mg/L, 8 mg/L, and 16 mg/L, untreated control group, and cell culture without cells. Cytotoxicity was evaluated after exposure for 5-min exposure using Mosmann’s Tetrazolium Toxicity (MTT) assay at 0 h and 48 h time points. Data were analyzed using a repeated measures analysis of variance and Post-hoc tests were performed using Bonferroni correction for multiple comparisons. Results All experimental groups showed proliferation at 0 h time point. However, all groups also experienced a decrease in overtime at 48 h time point (p < 0.05). At both time points 2 mg/L OW showed the highest cell viability as well as proliferation. At 0 h time point, the increase in cell viability for all experimental groups was found statistically significant when compared to positive control group (p < 0.05). At 48 h time point, although 8 mg/L and 16 mg/L OW showed statistically significant reduction in compare to 0 h time point, 2 mg/L and 4 mg/L OW groups didn’t experience any statistically significant difference (p < 0.05). Conclusion Considering our findings, due to ozonated water's induced a higher proliferation rate of dental pulp cells, indicating their biocompatibility and a possible adjuvant on irrigating agent in regenerative endodontic procedures.

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