scholarly journals Quantification of reaction cycle parameters for an essential molecular switch in an auxin-responsive transcription circuit in rice

2019 ◽  
Vol 116 (7) ◽  
pp. 2589-2594 ◽  
Author(s):  
Lucila Andrea Acevedo ◽  
Jeahoo Kwon ◽  
Linda K. Nicholson
Keyword(s):  
Lateral Root ◽  
Nmr Studies ◽  
Lateral Root Development ◽  
Isomerization Rate ◽  
Targeted Mutation ◽  
Essential Components ◽  
Wide Range ◽  
The Individual ◽  
Peptidyl Prolyl Isomerases

Protein-based molecular switches play critical roles in biological processes. The importance of the prolyl cis−trans switch is underscored by the ubiquitous presence of peptidyl prolyl isomerases such as cyclophilins that accelerate the intrinsically slow isomerization rate. In rice, a tryptophan−proline (W-P) cis−trans switch in transcription repressor protein OsIAA11 along with its associated cyclophilin LRT2 are essential components in a negative feedback gene regulation circuit that controls lateral root initiation in response to the plant hormone auxin. Importantly, no quantitative characterizations of the individual (microscopic) thermodynamic and kinetic parameters for any cyclophilin-catalyzed W-P isomerization have been reported. Here we present NMR studies that determine and independently validate these parameters for LRT2 catalysis of the W-P motif in OsIAA11, providing predictive power for understanding the role of this switch in the auxin-responsive circuit and the resulting lateral rootless phenotype in rice. We show that the observed isomerization rate is linearly dependent on LRT2 concentration but is independent of OsIAA11 concentration over a wide range, and LRT2 is optimally tuned to maintain OsIAA11 at its cis−trans equilibrium to supply the slower downstream cis-specific proteasomal degradation with maximal OsIAA11 substrate. This indicates that accelerating the LRT2-catalyzed isomerization would not accelerate OsIAA degradation, whereas decreasing this rate via targeted mutation could reveal relationships between circuit dynamics and lateral root development. Moreover, we show that sequences flanking the highly conserved Aux/IAA W-P motif do not impact LRT2 catalysis, suggesting that the parameters determined here are broadly applicable across highly conserved cyclophilins and their Aux/IAA targets.

scholarly journals Industry 4.0 and their application in medicine and dentistry, as well as the fight against the COVID-19 pandemic

Tehnika ◽  
2021 ◽  
Vol 76 (4) ◽  
pp. 509-520
Author(s):  
Nemanja Majstorović ◽  
Vidosav Majstorović ◽  
Radivoje Mitrović ◽  
Žarko Mišković
Keyword(s):  
Detailed Analysis ◽  
Industry 4.0 ◽  
Review Paper ◽  
Treatment Time ◽  
Complete System ◽  
Research Directions ◽  
Wide Range ◽  
Review Study ◽  
The Individual

Basics: Industry 4.0 is a realized reality, which already today has a growing application in medicine and dentistry. So, at this moment, its application is especially important in the fight against covid-19. Aim of the paper: The purpose of this review paper is to understand the role of Industry 4.0 in the field of medicine and dentistry and to identify research directions, including future applications, especially for covid-19. Material and method: A detailed analysis of papers on Industry 4.0 in medicine, dentistry and related fields was performed and possible applications and directions of research were identified, especially for covid-19.Result: Through this review study, we identified different technologies, opportunities and research status of Industry 4.0 in the field of medicine, dentistry and covid-19 pandemic, through 19 areas of application. We can say that Industry 4.0 will become an essential part of tomorrow's smart factory of medical and dental devices. Conclusion: Industry 4.0 shows a wide range of possibilities for the production of newly adapted implants (medical and dental) and innovative tools and instruments for the field of medicine and dentistry. This model enables the creation of a digital clinic and digital office, with a complete system of medical and dental monitoring, which meets the individual requirements of the patient, thus creating the industry of medicine and dentistry with optimization of treatment time and costs. In the future, Industry 4.0 will create new capabilities and innovative protocols for the treatment and care of patients, both in medicine and dentistry.

scholarly journals Heat Shock Proteins Are Essential Components in Transformation and Tumor Progression: Cancer Cell Intrinsic Pathways and Beyond

2019 ◽  
Vol 20 (18) ◽  
pp. 4507 ◽  
Author(s):  
Lang ◽  
Guerrero-Giménez ◽  
Prince ◽  
Ackerman ◽  
Bonorino ◽  
...  
Keyword(s):  
Heat Shock ◽  
Cancer Cells ◽  
Stress Proteins ◽  
De Novo ◽  
Human Cancer ◽  
Essential Components ◽  
Wide Range ◽  
Cancer Types ◽  
Shock Proteins

Heat shock protein (HSP) synthesis is switched on in a remarkably wide range of tumor cells, in both experimental animal systems and in human cancer, in which these proteins accumulate in high levels. In each case, elevated HSP concentrations bode ill for the patient, and are associated with a poor outlook in terms of survival in most cancer types. The significance of elevated HSPs is underpinned by their essential roles in mediating tumor cell intrinsic traits such as unscheduled cell division, escape from programmed cell death and senescence, de novo angiogenesis, and increased invasion and metastasis. An increased HSP expression thus seems essential for tumorigenesis. Perhaps of equal significance is the pronounced interplay between cancer cells and the tumor milieu, with essential roles for intracellular HSPs in the properties of the stromal cells, and their roles in programming malignant cells and in the release of HSPs from cancer cells to influence the behavior of the adjacent tumor and infiltrating the normal cells. These findings of a triple role for elevated HSP expression in tumorigenesis strongly support the targeting of HSPs in cancer, especially given the role of such stress proteins in resistance to conventional therapies.

The Role of Cybersecurity Certifications

2022 ◽  
pp. 93-118
Author(s):  
Adrian Davis
Keyword(s):  
Information Security ◽  
Job Descriptions ◽  
Organizational Perspective ◽  
Wide Range ◽  
Real Value ◽  
The Individual ◽  
Security Certifications ◽  
Individual Information

The chapter looks at the burgeoning field of certification for individuals in the field of information security or cybersecurity. Individual information security certifications cover a wide range of topics from the deeply technical to the managerial. These certifications are used as a visible indication of an individual's status and knowledge, used to define experience and status, used in job descriptions and screening, and may define expectations placed on the individual. This chapter examines how these certifications are produced, the subjects they cover, and how they integrate and the various audiences to which the certifications are aimed. The role, the perceived and real value, and benefits of certification within the field of information security both from an individual and an organizational perspective are discussed. Finally, some conclusions on certification are presented.

Employees and sustainability: the role of incentives

2016 ◽  
Vol 31 (4) ◽  
pp. 820-836 ◽  
Author(s):  
Kimberly K Merriman ◽  
Sagnika Sen ◽  
Andrew J Felo ◽  
Barrie E Litzky
Keyword(s):  
Financial Performance ◽  
Employee Engagement ◽  
Status Quo ◽  
Organizational Sustainability ◽  
Content Type ◽  
Incentive Effects ◽  
Environmental Objectives ◽  
Wide Range ◽  
The Individual

Purpose – Organizational sustainability has become a priority on many corporate agendas. How to integrate sustainability efforts throughout the organization, however, remains a challenge. The purpose of this paper is to examine two factors that potentially enhance incentive effects on employee engagement in environmental objectives: explicit organizational values for sustainability and the performance objective’s complementarity with incented financial objectives. Design/methodology/approach – The authors employed a quasi-experimental design in which participants were randomly assigned to one of four conditions, including a status quo condition against which the treatments were contrasted. Participants (n=400) were comprised of a cross-section of US employees from a wide range of occupations and industries. A post hoc qualitative analysis provided additional insights. Findings – Incentive effects were enhanced (i.e. preference for the environmental objective was significantly higher) when the environmental project offered complementary benefits for financial objectives, but not when organization values emphasized sustainability. An entrenched status quo bias for financial performance was discerned among a subset of the sample. Research limitations/implications – Management scholars must pay close attention to the role of implicit norms for financial performance when investigating employee engagement in organizational sustainability efforts. From an applied perspective, framing sustainability objectives to emphasize financial benefits consistent with a financial mission may maximize employee engagement. Originality/value – This study contributes to understanding of organizational sustainability efforts at the individual employee level of analysis, a conspicuously small part of the organizational research surrounding this topic.

scholarly journals Place of the Muscovy state in the expansion of the Europeans to the East in the XVI–XVII centuries (for the statement of the problem)

2021 ◽  
Vol 27 (4) ◽  
pp. 19-28
Author(s):  
R. S. Astashkin
Keyword(s):  
Geographical Location ◽  
Research Problem ◽  
Historical Experience ◽  
Russian Territory ◽  
Russian State ◽  
Wide Range ◽  
The Individual ◽  
Scientific Value ◽  
In Transit

The article attempts to pose the research problem of the place of the Russian state in the process of the expansion of Europeans to the East in the period of the XVIXVII centuries. The actual basis of the proposed topic consists of the numerous attempts by the representatives of the conditional West to use the specifics of the geographical location of the then Russia in order to establish and further develop the contacts with the particular Asian states. The experience of the complex, consistent and comprehensive coverage of this problem today is practically absent both in the domestic and the foreign historiography. The study of the place and role of the Muscovy empire in the process of penetration of the Western European powers, commercial and clerical corporations to the East is possible on the basis of an analysis of a wide range of written sources of Russian and foreign origins. This publication includes an approximate plan (a program) for the subsequent study of the stated issues. It seems appropriate to highlight the independent thematic aspects, among which the following should be named exclusively: the characteristics of the individual routes connecting the Russian lands with the East, the circumstances and consequences of the European travels to Asia in transit through the Russian territory, the participation of the Asian side (in particular, Persia) in the processes and events under consideration. One of the central places in the research program should be occupied by the little-studied question of the Muscovy empires own position on the problem of the European-Asian transit. In addition, the analysis of the historical experience of the intercontinental dialogue directly in the dominions of the Russian tsars is of considerable interest. The article concludes about the unconditional scientific value, prospects and novelty of the formulated problem.

scholarly journals Phosphorylation of Serine114 of the transcription factor ABSCISIC ACID INSENSITIVE 4 is essential for activity

2020 ◽  
Author(s):  
Nadav Eisner ◽  
Tzofia Maymon ◽  
Ester Cancho Sanchez ◽  
Dana Bar-Zvi ◽  
Sagie Brodsky ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Stress Responses ◽  
Lateral Root ◽  
Map Kinases ◽  
Regulation Of Gene Expression ◽  
List Type ◽  
Lateral Root Development ◽  
Aba Insensitive

AbstractThe transcription factor ABA-INSENSITIVE(ABI)4 has diverse roles in regulating plant growth, including inhibiting germination and reserve mobilization in response to ABA and high salinity, inhibiting seedling growth in response to high sugars, inhibiting lateral root growth, and repressing light-induced gene expression. ABI4 activity is regulated at multiple levels, including gene expression, protein stability, and activation by phosphorylation. Although ABI4 can be phosphorylated at multiple residues by MAPKs, we found that S114 is the preferred site of MPK3. To examine the possible biological role of S114 phosphorylation, we transformed abi4-1 mutant plants with ABI4pro::ABI4 constructs encoding wild type (114S), phosphorylation-null (S114A) or phosphomimetic (S114E) forms of ABI4. Phosphorylation of S114 is necessary for the response to ABA, glucose, salt stress, and lateral root development, where the abi4 phenotype could be complemented by expressing ABI4(114S) or ABI4(S114E) but not ABI4(S114A). Comparison of root transcriptomes in ABA-treated roots of abi4-1 mutant plants transformed with constructs encoding the different phosphorylation-forms of S114 of ABI4 revealed that 85% of the ABI4-regulated genes whose expression pattern could be restored by expressing ABI4(114S) are down-regulated by ABI4. Over half of the ABI4-modulated genes were independent of the phosphorylation state of ABI4; these are enriched for stress responses. Phosphorylation of S114 was required for regulation of 35% of repressed genes, but only 17% of induced genes. The genes whose repression requires the phosphorylation of S114 are mainly involved in embryo and seedling development, growth and differentiation, and regulation of gene expression.HighlightsTranscription factor ABI4 is a substrate of MAP kinases.MPK3 preferentially phosphorylates Serine 114 of ABI4.Phosphorylated Serine 114 of ABI4 is required for the complementation of abi4 mutants.Phosphorylated ABI4 acts primarily as a repressor.

scholarly journals Introduction to the Special Issue on Role of Scalable Computing and Data Analytics in Evolution of Internet of Things

2020 ◽  
Vol 21 (1) ◽  
pp. 1-2
Author(s):  
Swaminathan JN ◽  
Gopi Ram ◽  
Sureka Lanka
Keyword(s):  
Internet Of Things ◽  
Data Analytics ◽  
New Technologies ◽  
The Internet ◽  
Real World Data ◽  
Scalable Computing ◽  
Private Data ◽  
Wide Range ◽  
The Individual

The evolution of Internet of Things has given way to a Smart World where there is an improved integration of devices, systems and processes in humans through all pervasive connectivity. Anytime, anywhere connection and transaction is the motto of the Internet of things which brings comfort to the users and sweeps the problem of physical boundary out of the way. Once it has come into the purview of developers, new areas have been identified and new applications have been introduced. Small wearables which can track your health to big automated vehicles which can move from one place to another self navigating without human intervention are the order of the day. This has also brought into existence a new technology called cloud, since with IoT comes a large number of devices connected to the internet continuously pumping data into the cloud for storage and processing. Another area benefited from the evolution of IoT is the wireless and wired connectivity through a wide range of connectivity standards. As with any technology, it has also created a lot of concerns regarding the security, privacy and ethics.   Data protection issues created by new technologies are a threat which has been recognized by developers, public and also the governing body long back. The complexity of the system arises because of the various sensors and technologies which clearly tell the pattern of the activities of the individual as well an organization making us threat prone. Moreover, the volume of the data in the cloud makes it too difficult to recognize the privacy requirement of the data or to segregate open data from private data. Data analytics is another technology which supposedly increases the opportunity of increasing business by studying this private data collected from IoT and exploring ways to monetize them. It also helps the individual by recognizing their priorities and narrowing their search. But the data collected are real world data and aggregation of this data in the cloud is an open invitation to the hackers to study about the behaviors of the individuals.   The special issues of Scalable Computing has attract related to the Role of Scalable Computing and Data Analytics in Evolution of Internet of Things has attracted 28 submissions from which were selected 12.    

The Role of Cybersecurity Certifications

2019 ◽  
pp. 222-248
Author(s):  
Adrian Davis
Keyword(s):  
Information Security ◽  
Job Descriptions ◽  
Organizational Perspective ◽  
Wide Range ◽  
Real Value ◽  
The Individual ◽  
Security Certifications ◽  
Individual Information

The chapter looks at the burgeoning field of certification for individuals in the field of information security or cybersecurity. Individual information security certifications cover a wide range of topics from the deeply technical to the managerial. These certifications are used as a visible indication of an individual's status and knowledge, used to define experience and status, used in job descriptions and screening, and may define expectations placed on the individual. This chapter examines how these certifications are produced, the subjects they cover, and how they integrate and the various audiences to which the certifications are aimed. The role, the perceived and real value, and benefits of certification within the field of information security both from an individual and an organizational perspective are discussed. Finally, some conclusions on certification are presented.

Origins

2020 ◽  
pp. 11-30
Author(s):  
Phillip Brown
Keyword(s):  
Human Capital ◽  
Adam Smith ◽  
Wealth Creation ◽  
Capital Theory ◽  
Ibn Khaldun ◽  
Wide Range ◽  
Human Labor ◽  
History Of ◽  
The Individual

This chapter discusses the history of human capital theory. Before the mid-twentieth century the idea of human capital had a checkered history. Ideas linking the role of human labor to wealth creation can be traced to the works of Aristotle, Ibn Khaldun, and Thomas Aquinas. The chapter examines the ideas posed by notable economic theorists and thinkers such as Adam Smith, Alfred Marshall, Theodore Schultz, and Gary Becker. It shows how the ideas developed by these thinkers extended to a wide range of issues concerning the relationship between education and the labor market. In turn, they were able to influence policy in such powerful ways that their legacy remains. Above all, their influence shaped the way education is viewed in many countries: as an investment in the economic fortunes of the individual and the nation. This view gradually emerged as the dominant one, but was triumphantly sealed by the advent of neoliberalism in the 1980s.

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