Suitability of six extraction methods for isolating a large quantity of high-quality RNA from New Zealand free-draining stony soil

2020 ◽  
pp. 1-11
Author(s):  
Anastasija Podolyan ◽  
Gwen-Aëlle Grelet
Keyword(s):  
New Zealand ◽  
Extraction Methods ◽  
High Quality ◽  
Stony Soil

scholarly journals Compositional Analysis and Aroma Evaluation of Feijoa Essential Oils from New Zealand Grown Cultivars

Molecules ◽  
2019 ◽  
Vol 24 (11) ◽  
pp. 2053 ◽  
Author(s):  
Yaoyao Peng ◽  
Karen Suzanne Bishop ◽  
Siew Young Quek
Keyword(s):  
New Zealand ◽  
Essential Oil ◽  
Essential Oils ◽  
Extraction Method ◽  
Compositional Analysis ◽  
Extraction Methods ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Active Compounds ◽  
Initial Comparison

Feijoa is an aromatic fruit and the essential oil from feijoa peel could be a valuable by-product in the juicing industry. An initial comparison of the essential oil extraction methods, steam-distillation and hydro-distillation, was conducted. The volatile compounds in the essential oils from four feijoa cultivars were identified and semi-quantified by GC-MS and the aroma active compounds in each essential oil were characterized using SPME-GC-O-MS. Hydro-distillation, with a material to water ratio of 1:4 and an extraction time of 90 min, was the optimized extraction method for feijoa essential oil. The Wiki Tu cultivar produced the highest essential oil yield among the four selected cultivars. A total of 160 compounds were detected, among which 90 compounds were reported for the first time in feijoa essential oils. Terpenes and esters were dominant compounds in feijoa essential oil composition and were also major contributors to feijoa essential oil aroma. Key aroma active compounds in feijoa essential oils were α-terpineol, ethyl benzoate, (Z)-3-hexenyl hexanoate, linalool, (E)-geraniol, 2-undecanone, 3-octanone, α-cubebene, and germacrene D. This is the first report on the optimization of the extraction method and the establishment of the aroma profile of feijoa essential oils, with a comparison of four New Zealand grown cultivars.

scholarly journals Safe Deliveries?: a Review of New Zealand's Midwifery Regulation through the Lens of the Health and Disability Commissioner

2021 ◽  
Author(s):  
◽  
Claire Sweetman
Keyword(s):  
New Zealand ◽  
Maternity Care ◽  
Poor Quality ◽  
Disability Services ◽  
Birth Experience ◽  
High Quality ◽  
Quality Healthcare ◽  
Fundamental Part ◽  
Main Consumer ◽  
Pregnancy And Birth

<p>Although birth is a fundamental part of the life process, competing factions within the health profession struggle to agree on the best way to deliver maternity services. Despite this long-standing tension, the midwifery-led model has dominated New Zealand’s maternity system for more than two decades with the majority of consumers expressing satisfaction with the care provided. Unfortunately for a small number of mothers and babies the pregnancy and birth experience is not a positive one and families are left suffering life-long, and often tragic, consequences. As one of the main consumer watchdogs in New Zealand, the Health and Disability Commissioner is charged with investigating claims of poor quality healthcare. This paper examines the central themes in the Commissioner’s reports on substandard midwifery practice and proposes a number of regulatory solutions to the issues involved. Working in unison, these amendments have the potential to ease the pressure placed on midwives; enhance interprofessional relationships; improve practitioner competence; and increase overall compliance with the Code of Health and Disability Services Consumers’ Rights. By implementing these changes, the New Zealand Government could safeguard valuable midwifery-based principles whilst still ensuring that high quality maternity care is provided to all of the country’s mothers and babies.</p>

scholarly journals The case for an ?Excellence in Scholarship for Australia? initiative

2010 ◽  
Vol 16 (4) ◽  
pp. 228 ◽  
Author(s):  
Mike Calver
Keyword(s):  
Higher Education ◽  
New Zealand ◽  
Research Priorities ◽  
Education Sector ◽  
Research Quality ◽  
Research Assessment ◽  
High Quality ◽  
High Quality Research ◽  
Quality Research

Only those truly cryptozoic for all of 2010 could have missed the bustle and concern created by the Australian Commonwealth?s Excellence in Research for Australia (ERA) initiative (http://www.arc.gov.au/era/default.htm). In common with other national research assessment exercises such as the RAE (UK) and PBRF (New Zealand), ERA is designed to assess research quality within the Australian higher education sector, identifying and rewarding those institutions and departments producing high-quality research. The linkages between achievement, recognition and reward have the potential to shape the research priorities and agendas of institutions and individual researchers.

scholarly journals Newspaper quality, content and competition in New Zealand

2014 ◽  
Vol 20 (1) ◽  
pp. 181 ◽  
Author(s):  
Matthew Gibbons
Keyword(s):  
Content Analysis ◽  
New Zealand ◽  
Investment Strategies ◽  
High Quality

Content analysis results show that investment strategies and newspaper quality vary between companies in New Zealand. Indeed, Allied Press’ Otago Daily Times prints considerably more news than Fairfax papers with similar circulations, and almost as much news as the Christchurch Press. The results also provide some support for the theory that produc­ing a relatively high quality newspaper, when quality is measured by the quantity of news published and number of journalists employed, is as­sociated with relatively high readership. Vigorous competition between companies for dominance in the Queenstown newspaper market suggests that less concentrated newspaper ownership could increase competition and benefit consumers in other parts of New Zealand. Although the results are from 2006, strong similarities between the newspapers surveyed and more recent issues of these titles means the results and policy lessons drawn are still relevant.

scholarly journals EXTRACTION METHODS OF CLEANING OF MOTOR FUEL

2019 ◽  
Vol 62 (10) ◽  
pp. 30-39
Author(s):  
Sabina A. Seidova
Keyword(s):  
Organic Solvents ◽  
Extraction Method ◽  
Motor Fuel ◽  
Extraction Methods ◽  
National Academy Of Sciences ◽  
Selective Solvent ◽  
High Quality ◽  
Motor Fuels ◽  
Temperature And Pressure ◽  
Academy Of Sciences

In paper the results of the carried out analysis of literary data on preparation of motor fuels of high quality by extraction method of purification of the corresponding oil distillates with use of compounds of various class as a selective solvent have been presented. In particular, the results of comparative analysis of existing methods of the extraction purification of distillates of motor fuels from unnecessary components – aromatic hydrocarbons, sulphur-containing compounds and resinous substances with use of organic solvents and ion-liquid compositions as a selective solvent have been presented. The advantage of the extraction method of purification of motor fuels determined by possibility of the process at low temperature and pressure, by absence of necessity of application of the expensive catalysts, by possibility of regeneration and reuse of solvent, etc. in comparison with widely used hydrogenation method has been shown. The lacks of the organic solvents used as an extractant have been also listed and due to the ecological problems the use of non-volatile, thermally stable ion-liquid compositions as a selective solvent in the processes of purification of the distillates, intended for preparation a high quality target products, such as diesel fuel, gasoline, base oils for various purposes has been substantiated. In paper the results of systematic investigations carried out at the Institute of Petrochemical Processes of Azerbaijan National Academy of Sciences with the participation of the authors themselves, concerning the selective purification of the oil fractions of various composition and viscosity with use of ionic liquids synthesized on the basis of formic and acetic acids composition have been also presented. By carried out analysis it has been shown the perspectivity of application of the ion-liquid compositions as an extractant in the processes of the selective purification of the oil distillates.

scholarly journals Optimization of high molecular weight DNA extraction methods in shrimp for a long-read sequencing platform

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10340
Author(s):  
Pacharaporn Angthong ◽  
Tanaporn Uengwetwanit ◽  
Wirulda Pootakham ◽  
Kanchana Sittikankaew ◽  
Chutima Sonthirod ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Genomic Dna ◽  
Extraction Methods ◽  
Marine Organisms ◽  
High Quality ◽  
Sequencing Platform ◽  
Global Food Security ◽  
Long Read ◽  
Ctab Method ◽  
Global Food

Marine organisms are important to global food security as they are the largest source of animal proteins feeding mankind. Genomics-assisted aquaculture can increase yield while preserving the environment to ensure sufficient and sustainable production for global food security. However, only few high-quality genome sequences of marine organisms, especially shellfish, are available to the public partly because of the difficulty in the sequence assembly due to the complex nature of their genomes. A key step for a successful genome sequencing is the preparation of high-quality high molecular weight (HMW) genomic DNA. This study evaluated the effectiveness of five DNA extraction protocols (CTAB, Genomic-tip, Mollusc DNA, TIANamp Marine Animals DNA, and Sbeadex livestock kits) in obtaining shrimp HMW DNA for a long-read sequencing platform. DNA samples were assessed for quality and quantity using a Qubit fluorometer, NanoDrop spectrophotometer and pulsed-field gel electrophoresis. Among the five extraction methods examined without further optimization, the Genomic-tip kit yielded genomic DNA with the highest quality. However, further modifications of these established protocols might yield even better DNA quality and quantity. To further investigate whether the obtained genomic DNA could be used in a long-read sequencing application, DNA samples from the top three extraction methods (CTAB method, Genomic-tip and Mollusc DNA kits) were used for Pacific Biosciences (PacBio) library construction and sequencing. Genomic DNA obtained from Genomic-tip and Mollusc DNA kits allowed successful library construction, while the DNA obtained from the CTAB method did not. Genomic DNA isolated using the Genomic-tip kit yielded a higher number of long reads (N50 of 14.57 Kb) than those obtained from Mollusc DNA kits (N50 of 9.74 Kb). Thus, this study identified an effective extraction method for high-quality HMW genomic DNA of shrimp that can be applied to other marine organisms for a long-read sequencing platform.

Extraction of Lipid-Soluble Marine Biotoxins

1995 ◽  
Vol 78 (2) ◽  
pp. 480-482 ◽  
Author(s):  
Donald J Hannah ◽  
Desmond G Till ◽  
Terry Deverall ◽  
Paul D Jones ◽  
Joanne M Fry
Keyword(s):  
New Zealand ◽  
Saline Solution ◽  
Extraction Methods ◽  
New Method ◽  
Mouse Bioassay ◽  
Shellfish Poisoning ◽  
Constant Weight ◽  
Lipophilic Compounds ◽  
Safe Method ◽  
Marine Biotoxins

Abstract A recent extensive outbreak of toxic shellfish poisoning (TSP) in New Zealand, with at least 4 types of toxicities present, required the development of a new method for detecting lipid-soluble marine biotoxins. The complexity of studying this outbreak, requiring large sample numbers, dictated the development of a robust and safe method for extracting lipid-soluble toxins. The new method is based on extraction of lipophilic compounds with acetone followed by partitioning into dichloromethane. The dichloromethane extract is evaporated to constant weight and suspended in a detergent–saline solution for use in a mouse bioassay. The new method produces an extract of superior quality, is quicker and more sensitive compared with extraction methods currently used.

scholarly journals Brucellosis and tuberculosis in cattle in South America

2018 ◽  
Vol 55 (2) ◽  
pp. e141139
Author(s):  
José Soares Ferreira Neto
Keyword(s):  
New Zealand ◽  
South America ◽  
Epidemiological Data ◽  
Review Article ◽  
South American ◽  
Cattle Population ◽  
High Quality ◽  
The World ◽  
Meat Market ◽  
Epidemiological Situation

In general, European and North American countries, as well as Australia and New Zealand, have already eradicated or reached good levels of control of brucellosis and tuberculosis in cattle. In the rest of the world, however, the epidemiological situation of these two diseases is frequently poorly understood. In this review article, quantified data on these diseases in the South American countries are presented. Initially, the aspects that led the continent to host 25% of the world cattle population are presented, in addition to the aspects that placed the continent at a prominent position in the international meat market. Subsequently the continent was divided into three country groups, considering the size of the cattle population and how well the epidemiological situation of brucellosis and tuberculosis in cattle is quantified. It is argued that countries that do not generate high-quality quantitative epidemiological data on these diseases have serious limitations in outlining and managing control or eradication strategies. Thus, for successful outcomes, at least methodologies to estimate the prevalence of infected herds should be employed.

Value of chicory crops to pasture-based dairy farms in the Waikato region of New Zealand

2014 ◽  
Vol 153 (1) ◽  
pp. 124-137 ◽  
Author(s):  
A. J. ROMERA ◽  
G. J. DOOLE ◽  
E. N. KHAEMBAH
Keyword(s):  
New Zealand ◽  
Linear Optimization ◽  
Dairy Farms ◽  
High Quality ◽  
Pasture Production ◽  
Relative Growth ◽  
Increase Milk Production ◽  
Broad Variation ◽  
Milk Solids ◽  
To Receive

SUMMARYChicory (Cichorium intybusL.) crops can increase milk production on New Zealand dairy farms through providing high-quality feed in late lactation. Non-linear optimization models of three Waikato dairy farms, differing in the degree to which imported supplement is used, were employed to evaluate chicory crops in this environment. At a baseline milk price of $NZ 7/kg milk solids, it was only profitable for chicory to be used on a farm where no imported supplement was fed. However, even then, only 0·04 of the farm area was planted and profit only increased by $NZ 30/ha (0·006 of baseline profit), relative to where it was not used. It was not optimal to plant any chicory on farms where imported feed was available, at the baseline milk price and cost of establishment considered here. This was evident because imported feed is more flexible than chicory for filling temporary feed gaps; also its use does not displace pasture production. Sensitivity analysis indicated that the unprofitability of chicory is robust to broad variation in calving date and the relative growth of chicory and pasture. Overall, results indicate that farmers are unlikely to receive adequate reward for the additional complexity arising from the utilization of chicory crops, especially when imported supplement provides more flexibility.

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