THE SEX- AND AGE-INDEPENDENT EXPANSION OF THE FINGER TIPS IN LANZARANA LARGENI (LANZA, 1978): A NEW PHENOMENON IN AMPHIBIANS (SALIENTIA RANIDAE)

Colorectal cancer (CRC) is one of the most common types of cancer among men and women worldwide. Efforts are currently underway to find novel and more cancer-specific biomarkers that could be detected in a non-invasive way. The analysis of aberrant glycosylation of serum glycoproteins is a way to discover novel diagnostic and prognostic CRC biomarkers. The present study investigated a whole-serum glycome with a panel of 16 different lectins in search for age-independent and CRC-specific glycomarkers using receiver operating characteristic (ROC) curve analyses and glycan heat matrices. Glycosylation changes present in the whole serum were identified, which could lead to the discovery of novel biomarkers for CRC diagnostics. In particular, the change in the bisecting glycans (recognized by Phaseolus vulgaris erythroagglutinin) had the highest discrimination potential for CRC diagnostics in combination with human L selectin providing area under the ROC curve (AUC) of 0.989 (95% CI 0.950–1.000), specificity of 1.000, sensitivity of 0.900, and accuracy of 0.960. We also implemented novel tools for identification of lectins with strong discrimination power.

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Open quotient (EGG) measurements of young and elderly voices: results of a production and perception study

ZAS Papers in Linguistics ◽

10.21248/zaspil.40.2005.267 ◽

2005 ◽

Vol 40 ◽

pp. 213-225

Author(s):

Ralf Winkler ◽

Walter Sendlmeier

Keyword(s):

The Elderly ◽

Positive Relation ◽

Male And Female ◽

Elderly Males ◽

Significant Difference ◽

Vocal Effort ◽

Quantitative Results ◽

The Mean ◽

Perceived Age ◽

Age Independent

This paper presents the results of Open Quotient measurements in EGG signals of young (18 to 30 year old) and elderly (59 to 82 year old) male and female speakers. The paper further presents quantitative results on the relation between the OQ and the perception of a speaker's age. Higgins & Saxman (1991) found a decreased OQEGG with increasing age for females, whereas the OQEGG in sustained vowel material increased for males as the speakers age increased. In Linville (2002), however, the spectral amplitudes in the region of F0 (obtained by LTAS-measurements of read speech material) increased with increasing age independent of gender; this could be interpreted indirectly as an increasing OQ. We measured the OQEGG not only for sustained vowels, but also in vowels taken from isolated words. In order to analyse the relation between breathiness in terms of an increased OQ and the mean perceived age per stimulus a perception test was carried out in which listeners were asked to estimate speaker's age based on sustained /a/-vowel stimuli varying in vocal effort (soft - normal - loud) during production. The results indicated the following: (i) The decreased OQ for elderly females originally found by Higgins & Saxman is not apparent in our data for sustained /a/-vowels. For our female speakers no significant difference between the OQ of young and old speakers was found; for elderly males, however, we also found an increasing OQ with increasing age.(ii) In addition, a statistically significant increased OQEGG occurs for the group of the elderly males for the vowels from the word material. (iii) Our results show a strong positive relation between perceived age and OQ in male voices. Regarding (i) and (ii), at least the male speaker's voice becomes more breathy as age increases. Considering (iii), increased breathiness may contribute to the listener’s perception of increased age.

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Distorted TCR repertoires define multisystem inflammatory syndrome in children

10.1101/2021.04.12.21255098 ◽

2021 ◽

Author(s):

Amna Malik ◽

Eszter N. Tóth ◽

Michelle S. Teng ◽

Jacob Hurst ◽

Eleanor Watt ◽

...

Keyword(s):

T Cell ◽

Severe Acute Respiratory Syndrome ◽

Severe Disease ◽

Clinical Manifestations ◽

High Confidence ◽

T Cell Clones ◽

Severity Of Disease ◽

Cell Clones ◽

Inflammatory Syndrome ◽

Independent Expansion

While the majority of children infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) display mild or no symptoms, rare individuals develop severe disease presenting with multisystem inflammatory syndrome (MIS-C). The reason for variable clinical manifestations is not understood. Here, we carried out TCR sequencing and conducted comparative analyses of TCR repertoires between children with severe (n=12) or mild (n=8) COVID-19. We compared these repertoires with unexposed individuals (samples collected pre-COVID-19 pandemic: n=8) and with the Adaptive Biotechnologies MIRA dataset, which includes over 135,000 high-confidence SARS-CoV-2-specific TCRs. We show that the repertoires of severely ill children are characterised by the expansion of TRBV11-2 chains with high junctional and CDR3 diversity. Moreover, the CDR3 sequences of TRBV11-2 clones shift away from SARS-CoV-2 specific T cell clones, resulting in distorted TCR repertoires. In conclusion, our study reports that CDR3-independent expansion of TRBV11-2+ cells, lacking SARS-CoV-2 specificity, defines severity of disease in children.

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