Experimental and Numerical Investigation of the Characteristics of Spray-Freeze Drying for Various Parameters: Effects of Product Height, Heating Plate Temperature, and Wall Temperature

2010 ◽  
Vol 28 (2) ◽  
pp. 165-179 ◽  
Author(s):  
Geum-Su Yeom ◽  
Chi Sung Song
2020 ◽  
Vol 58 (1) ◽  
pp. 38-48
Author(s):  
Hilal Isleroglu ◽  
Izzet Turker

In this study, a commercial transglutaminase enzyme was dried using an ultrasonic spray freeze drying method and the effects of the process conditions were optimized to maximize the final transglutaminase activity. Accordingly, process parameters affecting enzyme activity were selected, such as nozzle frequency (48 and 120 kHz), flow rate (2, 5 and 8 mL/min) and plate temperature for secondary drying (25, 35 and 45 °C). Moreover, the effects of different pH values (pH=2.0 and 9.0) and high temperature (80 °C) on enzyme activity, physical properties and particle morphology of transglutaminase were discussed. According to the results, transglutaminase preserved its activity despite ultrasonic spray freeze drying. Sonication enhanced the enzyme activity. Using the desirability function method, the optimum process conditions were determined to be flow rate 3.10 mL/min, plate temperature 45 °C and nozzle frequency 120 kHz. The predicted activity ratio was 1.17, and experimentally obtained ratio was 1.14±0.02. Furthermore, enzyme produced by ultrasonic spray freeze drying had low moisture values (2.92-4.36 %) at 8 h of drying. When the morphological structure of the transglutaminase particles produced by ultrasonic spray freeze drying under the optimum conditions was examined, spherical particles with pores on their surfaces were observed. In addition, flow properties of the transglutaminase powders were considered as fair under most conditions according to the Carr index.


2014 ◽  
Vol 513-517 ◽  
pp. 4281-4284
Author(s):  
Chen Ji ◽  
Yan Li Fan ◽  
Gui Shan Liu ◽  
Wei Wang ◽  
Rui Ming Luo

In this paper, the effects of drying chamber pressure, heating plate temperature and material thickness on the drying time of Tan lamb in vacuum freeze-drying process were studied using quadratic regression orthogonal design. The results showed that the drying time was significantly affected by drying chamber pressure, heating plate temperature and material thickness as well as the interaction of heating plate temperature and material thickness. The optimized parameters were drying chamber pressure 27.9 Pa, heating plate temperature 47.9°C and material thickness 4.3 mm. On these parameters, the drying time was 4.3 h.


Author(s):  
B. Brahmini ◽  
L. Edukondalu ◽  
Venkata S. P. Bitra ◽  
G. Veeraprasad

Button mushroom (AgaricusbisporusL.) is extensively produced and consumed in the world. They are more perishable due to their high moisture content. Due to its short shelf-life, the mushroom is usually dehydrated for preservation. Hot air dried mushrooms result in losses in nutrients, colour degradation and deformation in structure. To overcome these problems, freeze-drying of mushroom slices was investigated. White button mushrooms after cleaning were vertically cut into 2, 4, 6 and 8 mm thick slices. Sliced mushrooms were frozen at −20 °C and then subjected to the freeze-drying at various heating plate temperatures of 10, 20, 30 and 40 °C. The effect of slice thickness and heating plate temperature on physicochemical properties like rehydration, porosity, firmness, water activity, colour, ascorbic acid, protein and microbial properties like total bacterial, yeast and mould were evaluated during the storage. Increase in the storage period resulted in decrease in porosity (73.25%), colour L* value (48.12), firmness (0.98 N), rehydration ratio (4.04), ascorbic acid content (14.47 mg/100 g) and protein content (19.15%), whereas the water activity (0.412) increased with the storage period. This may be due to the absorption of moisture during storage. Microbial analysis indicated by yeast count, mould count and total plate count was nil during the first three weeks of storage, whereas in the fourth week negligible growth was observed. So it is concluded that this may be due to the low water activity of stored mushroom slices.


npj Vaccines ◽  
2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Changjiao Gan ◽  
Wenbo Luo ◽  
Yunzhou Yu ◽  
Zhouguang Jiao ◽  
Sha Li ◽  
...  

AbstractBotulinum neurotoxin (BoNT), produced by Clostridium botulinum, is generally known to be the most poisonous of all biological toxins. In this study, we evaluate the protection conferred by intratracheal (i.t.) inoculation immunization with recombinant Hc subunit (AHc) vaccines against aerosolized BoNT/A intoxication. Three AHc vaccine formulations, i.e., conventional liquid, dry powder produced by spray freeze drying, and AHc dry powder reconstituted in water are prepared, and mice are immunized via i.t. inoculation or subcutaneous (s.c.) injection. Compared with s.c.-AHc-immunized mice, i.t.-AHc-immunized mice exhibit a slightly stronger protection against a challenge with 30,000× LD50 aerosolized BoNT/A. Of note, only i.t.-AHc induces a significantly higher level of toxin-neutralizing mucosal secretory IgA (SIgA) production in the bronchoalveolar lavage of mice. In conclusion, our study demonstrates that the immune protection conferred by the three formulations of AHc is comparable, while i.t. immunization of AHc is superior to s.c. immunization against aerosolized BoNT/A intoxication.


Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1023
Author(s):  
Ji Young Yu ◽  
Piyanan Chuesiang ◽  
Gye Hwa Shin ◽  
Hyun Jin Park

Liposomes have been utilized as a drug delivery system to increase the bioavailability of drugs and to control the rate of drug release at the target site of action. However, the occurrence of self-aggregation, coalescence, flocculation and the precipitation of aqueous liposomes during formulation or storage can cause degradation of the vesicle structure, leading to the decomposition of liposomes. To increase the stability of liposomes, post-processing techniques have been applied as an additional process to liposomes after formulation to remove water and generate dry liposome particles with a higher stability and greater accessibility for drug administration in comparison with aqueous liposomes. This review covers the effect of these techniques including freeze drying, spray drying and spray freeze drying on the stability, physicochemical properties and drug encapsulation efficiency of dry liposomes. The parameters affecting the properties of liposomes during the drying process are also highlighted in this review. In addition, the impact of using a protective agent to overcome such limitations of each process is thoroughly discussed through various studies.


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