A COMPARISON OF COMPUTERIZED IMAGE ANALYSIS AND STEREOLOGY AS TOOLS FOR MORPHOLOGICAL STUDY OF ALGAL CELLS

A computerized image analysis package (ImagePro+) was evaluated as an alternative method for morphometric analysis of electron micrographs of microalgal cells. The morphometric analysis was demonstrated with micrographs of the marine alga Nannochloropsis sp. grown under high and low light intensity. We applied the ImagePro+ package to estimate the relative volume of an organelle based on the ratio of perimeters of the organelle and the cell. The measurements included the volumes of chloroplasts, mitochondria, nuclei, vacuoles, and accumulation bodies, all relative to cell volume. The length of thylakoids was measured using the same package. The results obtained by ImagePro+ were compared to those of the traditional manual and laborious method involving the superimposition of an array of short lines on the micrograph. A high correlation between the methods was found. The following correlations were found for chloroplast, nucleus, and accumulation bodies: 0.96, 0.92, and 0.75, respectively. The correlation between length of thylakoids (ImagePro+) and surface area of thylakoids (superimposition) was 0.82.

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Pendugaan Parameter Genetik Kedelai Generasi F4 pada Intensitas Cahaya Rendah

Agrotek ◽

10.30862/agt.v2i3.472 ◽

2018 ◽

Vol 2 (3) ◽

Author(s):

Yohanis Amos Mustamu ◽

Trikoesoemaningtyas Trikoesoemaningtyas ◽

Desta Wirnas ◽

Didy Sopandie ◽

Darman M. Arsyad

Keyword(s):

Light Intensity ◽

Genetic Parameter ◽

Design Experiment ◽

Low Light ◽

Experimental Field ◽

High Heritability ◽

Intensity Condition ◽

Augmented Design ◽

Genetic Coefficient ◽

The University

The objective of this study was to collect information on genetic parameter and agronomy character of soybean F4 generation in the low light intensity condition. The parameter was tested to 130 lines F4 which are produced by Balai Besar Pengkajian dan Pengembangan Teknologi Pertanian (BBP2TP) Boor and the genotype of Sibayak, Tegal, Tanggamus, and Argomulyo were used as controls. The experiment was conducted in the university�s experimental field in Cikabayan, from September to December 2007. A total of 130 advance (F4) soybean lines were evaluated under shading in an augmented design experiment. The result of this study showed that all character has low genetic coefficient. The weight character of 25 grains has a considerably high heritability number in low li

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Application of Image Analysis System for Study of Activated Sludge Flocs

Water Quality Research Journal ◽

10.2166/wqrj.1986.010 ◽

1986 ◽

Vol 21 (1) ◽

pp. 130-140 ◽

Cited By ~ 4

Author(s):

Da-hong Li ◽

J. J. Ganczarczyk

Keyword(s):

Image Analysis ◽

Activated Sludge ◽

Statistical Processing ◽

Equivalent Diameter ◽

Image Analysis System ◽

Sectional Area ◽

Cross Sectional ◽

Computerized Image Analysis ◽

Sludge Flocs ◽

Analysis System

Abstract The computerized image analysis system has been successfully used for determination and statistical processing of the following geometric characteristics of activated sludge flocs: longest dimension, breadth, equivalent diameter, cross-sectional area, perimeter, elongation, and circularity. These parameters could be effectively and precisely determined by the system applied. In addition, the studied method, as compared to direct microscope observation and photography floc-sizing methods, was found to be more accurate, less time-consuming, and less dependent on the investigators.

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Growth of Haematococcus pluvialis on a Small-Scale Angled Porous Substrate Photobioreactor for Green Stage Biomass

Applied Sciences ◽

10.3390/app11041788 ◽

2021 ◽

Vol 11 (4) ◽

pp. 1788

Author(s):

Thanh-Tri Do ◽

Binh-Nguyen Ong ◽

Tuan-Loc Le ◽

Thanh-Cong Nguyen ◽

Bich-Huy Tran-Thi ◽

...

Keyword(s):

Light Intensity ◽

Algal Biomass ◽

Haematococcus Pluvialis ◽

Biomass Productivity ◽

Pilot Scale ◽

Small Scale ◽

Porous Substrate ◽

Low Light ◽

Green Algal ◽

Green Stage

In the production of astaxanthin from Haematococcus pluvialis, the process of growing algal biomass in the vegetative green stage is an indispensable step in both suspended and immobilized cultivations. The green algal biomass is usually cultured in a suspension under a low light intensity. However, for astaxanthin accumulation, the microalgae need to be centrifuged and transferred to a new medium or culture system, a significant difficulty when upscaling astaxanthin production. In this research, a small-scale angled twin-layer porous substrate photobioreactor (TL-PSBR) was used to cultivate green stage biomass of H. pluvialis. Under low light intensities of 20–80 µmol photons m−2·s−1, algae in the biofilm consisted exclusively of non-motile vegetative cells (green palmella cells) after ten days of culturing. The optimal initial biomass density was 6.5 g·m−2, and the dry biomass productivity at a light intensity of 80 µmol photons m−2·s−1 was 6.5 g·m−2·d−1. The green stage biomass of H. pluvialis created in this small-scale angled TL-PSBR can be easily harvested and directly used as the source of material for the inoculation of a pilot-scale TL-PSBR for the production of astaxanthin.

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Movement Characteristics of Ejaculated Sperm From Cynomolgus Monkeys ( Macaca fascicularis ) Analyzed by Manual and Automated Computerized Image Analysis

Journal of Medical Primatology ◽

10.1111/j.1600-0684.1989.tb00204.x ◽

1989 ◽

Vol 18 (1) ◽

pp. 55-68 ◽

Cited By ~ 1

Author(s):

C.H. Yeung ◽

G.F. Weinbauer ◽

E. Nieschlag

Keyword(s):

Image Analysis ◽

Macaca Fascicularis ◽

Cynomolgus Monkeys ◽

Computerized Image Analysis ◽

Movement Characteristics

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Transfer of photosynthetically fixed carbon between the prokaryotic green alga Prochloron and its ascidian host

Marine and Freshwater Research ◽

10.1071/mf9830431 ◽

1983 ◽

Vol 34 (3) ◽

pp. 431 ◽

Cited By ~ 21

Author(s):

DJ Griffiths ◽

L Thinh

Keyword(s):

Light Intensity ◽

Green Alga ◽

Host Tissue ◽

Total Carbon ◽

Symbiotic Association ◽

Fixed Carbon ◽

Low Light ◽

Ascidian Species ◽

Dark Fixation ◽

Efficient Transfer

In the symbiotic association between the prokaryotic green alga Prochloron and three didemnid host species (Diplosoma similis, Lissoclinum bistratum, Trididemnum cyclops), between 6 and 51 % of the total carbon fixed during exposure for 1 h to H14CO3- in the light (150 �E m-2 s-1) becomes associated with the host tissue. Dark fixation of 14CO2 in these ascidian species and in Lissoclinum punctatum never exceeds 6% of photosynthetic fixation at saturating light intensity. The corresponding values for dark fixation of 14CO2 in isolated Prochloron cells fall within the same range. There is very little excretion of photosynthate from whole colonies of the above ascidian species nor from Didemnum molle, Lissoclinum voeltzkowi and Trididemnum miniatum (usually less than 1 % of total photosynthate at saturation light intensity), suggesting an efficient transfer mechanism from Prochloron to host. Evidence from pulse-chase experiments suggests that transfer probably involves the early products of photosynthesis. The extent of transfer of photosynthate between Prochloron and T. cyclops varies with the rate of photosynthetic 14CO2 fixation into the whole colony but there is some transfer even at low light intensities, which strongly limit photosynthesis.

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Response of Photosynthesis and Chlorophyll Fluorescence Parameters of Castanopsis kawakamii Seedlings to Forest Gaps

Forests ◽

10.3390/f11010021 ◽

2019 ◽

Vol 11 (1) ◽

pp. 21

Author(s):

Zhong-sheng He ◽

Rong Tang ◽

Meng-jia Li ◽

Meng-ran Jin ◽

Cong Xin ◽

...

Keyword(s):

Chlorophyll Fluorescence ◽

Light Intensity ◽

Seedling Growth ◽

Group Treatment ◽

Control Group ◽

Low Light ◽

Chlorophyll Fluorescence Parameters ◽

Forest Gaps ◽

Low Light Intensity ◽

Weak Light

Light is a major environmental factor limiting the growth and survival of plants. The heterogeneity of the light environment after gap formation in forest influences the leaf chlorophyll contents, net photosynthetic rate (Pn), and chlorophyll fluorescence, thus influencing the growth and regeneration of Castanopsis kawakamii seedlings. The aim of this study was to explore the effects of weak light on the photosynthetic physiology of C. kawakamii seedlings in forest gaps and non-gaps. The results showed that (1) the contents of chlorophyll a (Chl-a), chlorophyll b (Chl-b), and total chlorophyll (Chl-T) in forest gaps were lower than in non-gaps. Seedlings tended to increase chlorophyll content to absorb light energy to adapt to low light intensity in non-gap environments. (2) The Pn values of C. kawakamii seedlings in forest gaps were significantly higher than in non-gaps, and forest gaps could improve the seedlings’ photosynthetic capacity. (3) The C. kawakamii seedlings in forest gaps were more sensitive to weak light and control group treatment, especially the tall seedlings, indicating that seedlings require more light to satisfy their growth needs in the winter. The seedlings in non-gaps demonstrated better adaptability to low light intensity. The light intensity was not adequate in weak light conditions and limited seedling growth. We suggest that partial forest selection cutting could improve light intensity in non-gaps, thus promoting seedling growth and regeneration of C. kawakamii more effectively in this forest.

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Nerve Growth Factor Enhances Nerve Regeneration through Fibronectin Grafts

Journal of Hand Surgery (European Volume) ◽

10.1016/s0266-7681(96)80058-1 ◽

1996 ◽

Vol 21 (4) ◽

pp. 514-522 ◽

Cited By ~ 77

Author(s):

I. H. WHITWORTH ◽

R. A. BROWN ◽

C. J. DORÉ ◽

P. ANAND ◽

C. J. GREEN ◽

...

Keyword(s):

Image Analysis ◽

Nerve Growth Factor ◽

Growth Factor ◽

Schwann Cell ◽

Nerve Regeneration ◽

Axonal Regeneration ◽

Cell Regeneration ◽

Nerve Growth ◽

Computerized Image Analysis ◽

Immunohistochemical Techniques

Soluble fibronectin and nerve growth factor (NGF) promote axonal regeneration when placed in silicone tubes. We investigated the ability of orientated fibronectin mats to bind and release bioactive NGF and the possibility of augmenting axonal regeneration following axotomy by using fibronectin conduits impregnated with NGF. The release of NGF was quantified using a fluorometric ELISA and bioactivity confirmed with a neuronal culture bioassay. Immunohistochemical techniques and computerized image analysis were used to assess the rate and volume of axonal and Schwann cell regeneration. The delivery of NGF to the site of injury produced an increase in the rate ( P≤0.007) and volume ( P≤0.004) of both axonal and Schwann cell regeneration when compared to conduits of plain fibronectin. We conclude that the local delivery of NGF by impregnated fibronectin conduits enhances axonal regeneration.

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A laboratory study of vertical migration

Proceedings of the Royal Society of London Series B Biological Sciences ◽

10.1098/rspb.1955.0062 ◽

1955 ◽

Vol 144 (916) ◽

pp. 329-354 ◽

Cited By ~ 24

Keyword(s):

Light Intensity ◽

Vertical Migration ◽

Time Course ◽

Tap Water ◽

Low Light ◽

Overhead Light ◽

Light Intensities ◽

Orientation Response ◽

Reduced Light ◽

Characteristic Maximum

In a tank filled with a suspension of indian ink in tap water, a population of Daphnia magna will undergo a complete cycle of vertical migration when an overhead light source is cyclically varied in intensity. A ‘dawn rise’ to the surface at low intensity is followed by the descent of the animals to a characteristic maximum depth. The animals rise to the surface again as the light decreases, and finally show a typical midnight sinking. The light intensities at the level of the animals in this experiment are of the same order as those which have been reported in field observations; the time course of the movement also repeats the natural conditions in the field. The process is independent of the duration of the cycle and is related only to the variation in overhead light intensity. At low light intensity the movement of the animal is determined solely by positive photo-kinesis; the dawn rise is a manifestation of this, and is independent of the direction of the light. At high light intensities there is an orientation response which is superimposed upon an alternating positive (photokinetic) phase and a negative phase during which movement is inhibited. The fully oriented animal shows a special type of positive and negative phototaxis, moving towards the light at reduced light intensities and away from it when the light intensity is increased. In this condition it follows a zone of optimum light intensity with some exactness. Experiments show that an animal in this fully oriented condition will respond to the slow changes of intensity characteristic of the diurnal cycle, while being little affected by transient changes of considerable magnitude.

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