scholarly journals Subpopulations of mononuclear leukocytes associated with inhibition of Ehrlich ascites tumor growth by treatment withBothrops jararacavenom

2004 ◽  
Vol 13 (1) ◽  
pp. 29-32 ◽  
Author(s):  
Mariana Morena de Vieira Santos ◽  
Reinaldo José da Silva ◽  
Márcia Guimarães da Silva ◽  
Denise Fecchio
Keyword(s):  
Natural Killer Cells ◽  
Natural Killer ◽  
Experimental Models ◽  
Ehrlich Ascites Tumor ◽  
Mononuclear Leukocytes ◽  
Control Group ◽  
Ascites Tumor ◽  
Growth Modulation ◽  
Killer Cells ◽  
Ehrlich Ascites

Snake venoms have been used as antineoplastic substances in several experimental models. We demonstrated in previous studies thatBothrops jararacavenom (BjV) induces inhibition of Ehrlich ascites tumor (EAT) growth accompanied by an increase of mononuclear (MN) leukocytes in all groups inoculated with EAT and/or venom. The objective of the present study was to characterize the subpopulations of MN leukocytes involved in the inhibition of EAT growth by treatment with BjV. Swiss mice were inoculated with 1.0×103EAT cells by the intraperitoneal route and treated with 0.4 mg/kg of BjV by the same route (Group TV). Treatment was started 24 h after tumor cell inoculation and consisted of five intraperitoneal injections performed at 72 h intervals. After 2, 8 and 14 days, groups of animals were sacrificed and the number of B, TCD4 and TCD8 lymphocytes, macrophages and natural killer cells present in the peritoneal cavity was determined by flow cytometry. The control group consisted of animals inoculated with EAT and treated with 0.1 ml of saline under the same conditions as the experimental group (Group T). Two additional control groups consisted of animals not inoculated with EAT and treated with saline or venom. Data were analyzed statistically by the Kruskal-Wallis non-parametric test for independent samples. On the 2nd and 8th day we observed a difference between groups T and TV (group T > group TV) for all cell types, except natural killer cells, that only differed on the 2nd day. However, on the 14th day there was no difference in MN cells among groups. These data suggest that the inhibition of EAT is related to the toxic action of BjV on tumor cells and/or to the proteolytic effect of the venom on the mediators produced by the cells for growth modulation.

In vivo Antitumoral Effect of Plantago major L. Extract on Balb/C Mouse with Ehrlich Ascites Tumor

2007 ◽  
Vol 35 (05) ◽  
pp. 841-851 ◽  
Author(s):  
Mehmet Ozaslan ◽  
I. Didem Karagöz ◽  
M. Emin Kalender ◽  
I. Halil. Kilic ◽  
Ibrahim Sari ◽  
...  
Keyword(s):  
Weight Gain ◽  
Negative Control Group ◽  
Negative Control ◽  
Ehrlich Ascites Tumor ◽  
Control Group ◽  
Plantago Major ◽  
Ascites Tumor ◽  
Treatment Groups ◽  
Ehrlich Ascites

The aim of this study is to investigate the antitumor activity of Plantago major L. extract in Ehrlich ascites tumor (EAT) bearing Balb/C mice in vivo. Thirty male Balb/C mice were divided into 5 groups: 3 treatment groups and 2 control groups (6 per group). Treatment groups and the negative control group were injected with EAT (1 × 106 cells) intraperitoneally to develop ascites tumor. P. major L. extract (1%, 2% and 3% concentration extracts, 0.1 ml/day/mouse) were given p.o. for 10 alternate days. The control group was treated with 0.9% NaCl solution (0.1 ml/day/mouse). The changes of body weight in animals were recorded. On the 11th day, all of the mice were sacrified and their tissues were stained with haematoxylen and eosin for pathological studies. Body weights of in 3 treatment groups and the negative control group were elevated because of tumor burden. The maximal weight gain was recorded in the negative control group and the minimal weight gain was recorded in Group I. Pathological studies showed that P. major L. extract (especially 1% concentration) has inhibitive effect on EAT. P. major has an inhibitory effect on EAT in a dose dependent manner.

scholarly journals Effect of Apatinib on Serum CD4+CD25+ T cells, NK Cells, and T Cells Subgroup in Malignant Tumor

2019 ◽  
Vol 18 ◽  
pp. 153303381989366
Author(s):  
Hui-Qin Luo ◽  
Yi-Fu He ◽  
Wen-Ju Chen ◽  
Ying Yan ◽  
Shu-Sheng Wu ◽  
...  
Keyword(s):  
T Cells ◽  
Prognostic Factors ◽  
Natural Killer Cells ◽  
Natural Killer ◽  
Progression Free Survival ◽  
Control Group ◽  
Killer Cells ◽  
Free Survival ◽  
Median Progression Free Survival ◽  
The Relationship

Objective: The immune makers including CD4+CD25+ T cells, natural killer cells, and T cells subgroup were retrospectively analyzed to find the relationship between apatinib and the immune system in the patients treated with apatinib. Method: Forty-two patients with advanced malignant tumors orally took apatinib as treatment and 16 patients with the same situation did not take apatinib as a control group. These patients were all included in the study, and they orally received apatinib 500 mg daily as monotherapy or combination. The treatment was continued until disease progression or intolerable toxicity. CD4+CD25+ T cells, natural killer cells, and T cells subgroup were detected before and 1 month after therapy for all the patients. The relationship between the changing number of immune cells and progression-free survival was analyzed in this study. Result: For the apatinib group, the rate of CD4+CD25+ T cells significantly increased ( P = .048). The median progression-free survival was 3.25 months for the 42 patients. The median progression-free survival in the patients with the rate of CD4+CD25+ T cells increased and decreased was 5.8 months and 2.9 months, respectively ( P = .012). Multivariate analysis found the increased rate of CD4+CD25+ T cells was an independent prognostic factor for a longer progression-free survival. The rate of natural killer cells and T cells subgroup did not change much after apatinib therapy, and they were not independent prognostic factors for progression-free survival. Conclusion: The rate of CD4+CD25+ T cells is very important in patients with apatinib treatment. The changing number of CD4+CD25+ T cells may be a good indicator for apatinib prognosis. Natural killer cells and T cells subgroup did not change much after apatinib, and they were not independent prognostic factors for progression-free survival.

scholarly journals The Lipiodol Uterine Bathing Effect to Improve Fertility May Include Uterine Natural Killer Cell Up-regulation in the Endometrium

2021 ◽  
pp. 1-4
Author(s):  
N. P. Johnson ◽  
S. Baidya ◽  
S. O. Jessup ◽  
A. Muthukaruppan ◽  
W. E. Hadden ◽  
...  
Keyword(s):  
T Cells ◽  
Natural Killer Cells ◽  
Natural Killer ◽  
Killer Cell ◽  
Randomised Trial ◽  
Control Group ◽  
Killer Cells ◽  
Uterine Natural Killer Cells ◽  
The Mean ◽  
Uterine Natural Killer

BACKGROUND: Lipiodol has a dramatic short term fertility enhancing effect for women with endometriosis. Microarray studies have shown transcriptomic regulation of molecular markers of endometrial inflammation, most notably a consistent down-regulation of endometrial osteopontin. We further explored the endometrial bathing effect of lipiodol on leukocyte expression in endometrium. METHODS: A cohort of four women, nested within a randomised trial of twelve women assessing the lipiodol uterine bathing effect, was studied as an ‘own control’ group, with their mid-luteal endometrium assessed before and after endometrial lipiodol exposure. Pipelle endometrial sampling allowed endometrial assessment by immunochemistry. Endometrial tissue samples were assessed by immunochemistry for total CD45+ leukocytes, CD68+ macrophages, CD3+ T-cells and CD56+ uterine natural killer cells. RESULTS: There was a statistically significant increase in the mean density of uterine natural killer cells in the endometrium of women post-lipiodol. No other significant differences were found in the mean densities of all leukocytes, macrophages or T cells in the endometrium of women post-lipiodol. CONCLUSIONS: These preliminary data further support the concept of a uterine bathing effect of lipiodol. Whether the increase in the mean density of uterine natural killer cells in the endometrium might contribute to an improvement in endometrial receptivity to embryo implantation merits further investigation.

The Profile Of The T Helper, Cytotoxic T and Natural Killer Cells In Sickle Cell Anemia Patients With Ischemic Attack and The Relation With Clinical Prognosis

Blood ◽  
2013 ◽  
Vol 122 (21) ◽  
pp. 4731-4731
Author(s):  
Yurdanur Kilinç ◽  
Bahriye - Atmis ◽  
Anil Atmis ◽  
Mustafa Yilmaz ◽  
Ilgen Sasmaz ◽  
...  
Keyword(s):  
T Cells ◽  
Steady State ◽  
Natural Killer Cells ◽  
Natural Killer ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Cytotoxic T Cells ◽  
Control Group ◽  
T Helper ◽  
Killer Cells

Purpose The study is conducted in 29 sickle cell anemia (SCA) patients, who are in ischemic attacks (in vasoocclusive crises period) and in steady state; the level of T helper cells, cytotoxic T cells and natural killer cells were determined and the effect of ischemic attack on clinical prognosis and the immune functions. Materials and Methods In the study, 29 patients with sickle cell anemia in ischemic attacks with painful crises and in steady state and 24 healthy children were chosen in the same age group for control group. These groups were examined with complete blood cells count, Hb electrophoreses and the blood biochemistry. The study was performed by flowcytometric method to find the level of CD3 monoclonal antibody for total T lymphocyte, CD4 monoclonal antibody for T helper cells, CD8 monoclonal antibody for cytotoxic T cells and CD16+56 monoclonal antibodies for natural killer cells in the mentioned groups to compare the statistical data. Findings The average of HbS in ischemic periods in SCA patients are to be found 83±6,6 %. There were decrease in hemoglobin and hematocrit levels in SCA versus control group (p<0,001). The levels of CD3 are lower in SCA patients with crises period (62,31±7,79 %) than in steady state (65,53±5,72 %) and in control group (69,09±9,18 %) (p=0,007). The natural killer cells in crisis period (13,07±7,67 %) and in steady state (12,71±5,62 %) were lower than in control group (8,11±4,67 %) (p=0,009). In SCA patients, the level of CD3,CD4, CD8 and CD16+56(+) T cells are not changed with the frequency of crisis (p>0,05). Results As a result of the study, in the patients with sickle cell anemia whom associated with chronic hemolysis and tissue hypoxia during the ischemic attack, the levels of total T lymphocyte (CD3) was found significantly lower than the control groupp<0.001. The levels of T helper cells (CD4) and cytotoxic T cells (CD8) did not differ between SCA patients and control group. The levels of natural killer cells (CD16+56) was found higher in crises period and steady state than control group. To find the T cell subset levels in clinical and prognostic effects in SCA, it is necessary to do study with bigger groups of patients and wide range of study. Disclosures: No relevant conflicts of interest to declare.

Abstract 578: Depletion Of Natural Killer Cells Is Associated With Deterioration Of Fetal Outcome In A Transgenic Rat Model For Preeclampsia

Hypertension ◽  
2014 ◽  
Vol 64 (suppl_1) ◽  
Author(s):  
Michaela Golic ◽  
Nadine Haase ◽  
Florian Herse ◽  
Lukasz Przybyl ◽  
Stefan Verlohren ◽  
...  
Keyword(s):  
Natural Killer Cells ◽  
Natural Killer ◽  
Rat Model ◽  
Fetal Outcome ◽  
Control Group ◽  
Transgenic Rat ◽  
Female Rat ◽  
Killer Cells ◽  
Asialo Gm1 ◽  
Transgenic Rat Model

Introduction: Preeclampsia is defined by maternal hypertension and proteinuria developed during pregnancy. It is associated with fetal growth restriction and diminished trophoblast cell invasion into uterus. Uterine natural killer cells are the most abundant leukocytes during early pregnancy. They are considered as regulators of placentation and remodeling of spiral arteries. However, their precise function remains still unclear and is contradictory. It has been shown that depletion of natural killer cells increases number of invasive trophoblast cells in healthy pregnant rats. We tested the hypothesis whether depletion of natural killer cells improves maternal symptoms and fetal outcome in preeclamptic rats. Material and Methods: We have developed and characterized a transgenic rat model for preeclampsia. After a female rat expressing the human angiotensinogen has been mated with a male transgenic for the human renin, the female develops hypertension and proteinuria during pregnancy. Its fetuses are growth restricted. Within this rat model, we depleted natural killer cells with the antibody anti-asialo GM1 according to a published protocol (intraperitoneal injections on day 5 and day 10 of pregnancy). We analyzed 7 preeclamptic rats with in total 72 fetuses receiving anti-asialo GM1 and 5 control rats with in total 54 fetuses receiving rabbit serum. Rats were culled at end of pregnancy (day 21). Results: Successful depletion of natural killer cells was shown by flow cytometry of the spleen and mRNA analysis of the uterus. Application of anti-asialo GM1 had no influence on blood pressure (158±8mmHg control group vs. 155±8mmHg anti-asialo GM1 group, mean arterial pressure on day 17, measured by telemetry), proteinuria (7.0±5.1mg/24hrs control group vs. 16.2±10.0mg/24hrs anti-asialo GM1 group), and fetal weight (2.9±0.6g control group vs. 2.7±0.5g anti-asialo GM1 group). However, fetal brain/liver weight ratio as a marker of intrauterine growth retardation was increased by 22% in fetuses of the anti-asialo GM1 group (0.9±0.2 control group vs. 1.1±0.4 anti-asialo GM1 group, p=0.02). Conclusion: Depletion of natural killer cells is not associated with altered maternal symptoms of the preeclamptic phenotype, but with deterioration of fetal outcome.

scholarly journals Laparoscopic Surgery Versus Open Surgery for Colorectal Cancer: Impacts on Natural Killer Cells

2020 ◽  
Vol 27 (1) ◽  
pp. 107327482090681 ◽  
Author(s):  
Liangpan Shi ◽  
Hailian Guo ◽  
Zhihua Zheng ◽  
Jiangrui Liu ◽  
Yancheng Jiang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Natural Killer Cells ◽  
Nk Cells ◽  
Natural Killer ◽  
Laparoscopic Resection ◽  
Laparoscopic Group ◽  
Control Group ◽  
Killer Cells ◽  
Before And After ◽  
The Impact

Background: Laparoscopic resection is increasingly used in colorectal cancer (CRC). It has been suggested to carry short-term benefits in safety, recovery, and preservation on immune function for patients with CRC. However, the impact of laparoscopic resection on natural killer (NK) cells is largely unclear. Methods: A total of 200 patients with CRC across Dukes A/B/C stages were randomly assigned to laparoscopic or open resection. The blood samples were collected before and after the surgery. The total number of NK cells was quantified by flow cytometer. Lytic units 35 toward K562 was used to quantify NK cells activity. The outcomes between the groups across pathological stages were also analyzed. Results: The number and activity of NK cells decreased after the surgery in both groups. The laparoscopic group showed a faster recovery rate of NK cells function than the control group as assessed by cell count and lytic activity. Natural killer cells were impaired in a higher degree in patients at Dukes B/C stages. The recovery of NK cells to baseline level at day 7 postsurgery was observed in the laparoscopic group across all 3 stages. Conclusion: Generally, laparoscopically assisted surgery resulted in a better preservation on NK cells function. A better outcome was observed in patients with CRC at Dukes B/C stages.

scholarly journals The effect of Esberitox on the functional activity of 2D-positive natural killer cells (NKG2D) and interferon status in patients with recurrence of herpes simplex virus infection

2020 ◽  
pp. 53-59
Author(s):  
Инна Пшеничная ◽  
Андрей Курченко
Keyword(s):  
Natural Killer Cells ◽  
Virus Infection ◽  
Natural Killer ◽  
Functional Activity ◽  
Control Group ◽  
Herpes Virus Infection ◽  
Killer Cells ◽  
Interferon Status ◽  
Herpès Virus ◽  
Observation Period

The aim of the study was to assess changes in the functional activity of 2D-positive natural killer cells (NKG2D) and interferon status in patients with recurrence of herpes virus infection under the influence of Esberitox.Materials and methods. We studied 30 patients aged 22-45 years old with frequent relapsing labial herpes, previously verified by PCR. The frequency ofexacerbations at the time of the study was 6 (5.3 ± 1.4) and more than once a year. Patients were initially examined within 24 hours of the appearance of rashes.The expression of CD314 on the surface of CD3-CD56 lymphocytes was determined by flow cytometry(EPICS XL, Beckman Coulter). The content of cytokines (α-and γ-IFN) in supernatants was determined by enzyme immunoassay (IBL, Germany). Patients took Esberitox tablets, 2 tablets x 3 times a day, and daily1-gram tablets of Valacyclovir for 7 days. The control group consisted of 10 patients receiving only tablet valacyclovir.Results. As a result of the study, it was found that the final leveling of symptoms with combined treatment with Esberitox was noted by the end of 5-6 days, with basic treatment - by 9-10 days. There was a decrease in the relapse rate (3 patients) during the observation period within 2 months after the end of the course of treatment.In patients receiving basic Valacyclovir therapy, relapses during the observation period were recorded in more than half of the examined individuals (6 patients). The positive dynamics of the increase in the level of γ-IFN and α-IFN in patients of the main group and itsalmost absence in the control group were established.The dynamics of surface expression of the NKG2D receptor on peripheral blood NK cells showed an increase parameters under the influence of the Esberitox drug compared with Valacyclovir monotherapy, with no significant changes in other lymphocyte subpopulations. Conclusions. The administration of Esberitox in case of frequently recurring herpes virus infection is a promising method of combined immuno-adaptive therapy, which requires further careful study.

scholarly journals The influence of different combinations of γ-linolenic acid, stearidonic acid and EPA on immune function in healthy young male subjects

2004 ◽  
Vol 91 (6) ◽  
pp. 893-903 ◽  
Author(s):  
Elizabeth A. Miles ◽  
Tapati Banerjee ◽  
Maaike M. B. W. Dooper ◽  
Laura M'Rabet ◽  
Yvo M. F. Graus ◽  
...  
Keyword(s):  
Natural Killer Cells ◽  
T Lymphocytes ◽  
Immune Function ◽  
Natural Killer ◽  
Linolenic Acid ◽  
Stearidonic Acid ◽  
Delayed Type Hypersensitivity ◽  
Control Group ◽  
Killer Cells ◽  
Male Subjects

To determine the effects of EPA, stearidonic acid (STA) or γ-linolenic acid (GLA) on immune outcomes, healthy male subjects consumed one of seven oil blends for 12 weeks. EPA consumption increased the EPA content of peripheral blood mononuclear cells (PBMC). Consumption of GLA (2·0 g/d) in the absence of STA or EPA increased di-homo-GLA content in PBMC. Neither STA nor its derivative 20:4n-3 appeared in PBMC when STA (<1·0 g/d) was consumed. However, STA (1·0 g/d), in combination with GLA (0·9 g/d), increased the proportion of EPA in PBMC. None of the treatments altered neutrophil or monocyte phagocytosis or respiratory burst, production of inflammatory cytokines by monocytes, T lymphocyte proliferation or the delayed-type hypersensitivity response. Production of cytokines by T lymphocytes increased in all groups, with no differences among them. The proportion of lymphocytes that were natural killer cells decreased significantly in subjects receiving 2·0 g EPA or GLA/d. There were no other effects on lymphocyte sub-populations. Plasma IgE concentration decreased in most groups, but not in the control group. Plasma IgG2 concentration increased in the EPA group. Thus, EPA or GLA at a dose of 2·0 g/d have little effect on key functions of neutrophils, monocytes and T lymphocytes, although at this dose these fatty acids decrease the number of natural killer cells. At this dose EPA increases IgG2 concentrations. STA can increase immune cell EPA status, but at 1·0 g/d does not affect human immune function.

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