Advancements of nature nanocage protein: preparation, identification and multiple applications of ferritins

2021 ◽  
pp. 1-12
Author(s):  
Han Li ◽  
Xiaoyu Xia ◽  
Xiaoyi Tan ◽  
Jiachen Zang ◽  
Zhenyu Wang ◽  
...  
Keyword(s):  
Protein Preparation

scholarly journals Membrane Protein Stabilization Strategies for Structural and Functional Studies

Membranes ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 155
Author(s):  
Ekaitz Errasti-Murugarren ◽  
Paola Bartoccioni ◽  
Manuel Palacín
Keyword(s):  
Membrane Proteins ◽  
Membrane Protein ◽  
Lipid Membrane ◽  
Protein Stabilization ◽  
New Drugs ◽  
Cell Physiology ◽  
Protein Preparation ◽  
Functional Studies ◽  
Membrane Protein Stability ◽  
Druggable Targets

Accounting for nearly two-thirds of known druggable targets, membrane proteins are highly relevant for cell physiology and pharmacology. In this regard, the structural determination of pharmacologically relevant targets would facilitate the intelligent design of new drugs. The structural biology of membrane proteins is a field experiencing significant growth as a result of the development of new strategies for structure determination. However, membrane protein preparation for structural studies continues to be a limiting step in many cases due to the inherent instability of these molecules in non-native membrane environments. This review describes the approaches that have been developed to improve membrane protein stability. Membrane protein mutagenesis, detergent selection, lipid membrane mimics, antibodies, and ligands are described in this review as approaches to facilitate the production of purified and stable membrane proteins of interest for structural and functional studies.

scholarly journals Proteome analysis of brain proteins in Alzheimer's disease: Subproteomics following sequentially extracted protein preparation

2004 ◽  
Vol 6 (3) ◽  
pp. 257-268 ◽  
Author(s):  
Aiko Shiozaki ◽  
Teruyuki Tsuji ◽  
Ryuichi Kohno ◽  
Jun Kawamata ◽  
Kengo Uemura ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Proteome Analysis ◽  
Protein Preparation ◽  
Brain Proteins

Safety assessment of Apoaequorin, a protein preparation: Subchronic toxicity study in rats

2013 ◽  
Vol 57 ◽  
pp. 1-10 ◽  
Author(s):  
Daniel L. Moran ◽  
Palma Ann Marone ◽  
Mark R. Bauter ◽  
Madhu G. Soni
Keyword(s):  
Safety Assessment ◽  
Toxicity Study ◽  
Subchronic Toxicity ◽  
Protein Preparation

Multiplexed Protein Preparation Systems for Proteomics

2002 ◽  
pp. 344-346
Author(s):  
Justine Taylor ◽  
Can Wang ◽  
D. Jed Harrison
Keyword(s):  
Protein Preparation

ALFALFA SUPPLEMENTATION OF CORNCOB DIETS FOR SHEEP: COMPARISON WITH HIGHER VOLATILE FATTY ACIDS, SOYBEAN PROTEIN AND ALFALFA FIBER ON INTAKE, ENVIRONMENT AND DIGESTION IN THE RUMEN AND DIGESTA PASSAGE

1987 ◽  
Vol 67 (4) ◽  
pp. 1083-1091 ◽  
Author(s):  
L. R. NDLOVU ◽  
J. G. BUCHANAN-SMITH
Keyword(s):  
Fatty Acids ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Soybean Protein ◽  
Control Diet ◽  
Basal Diet ◽  
Latin Squares ◽  
Protein Preparation ◽  
Alfalfa Hay

Nine lambs fitted with rumen cannulae were used in two 5 × 5 Latin squares to measure the effect of alfalfa hay, C4- and C5-branched- and straight-chain volatile fatty acids (C4/C5 VFAs), cell wall from alfalfa hay (ALFCW) and a soybean protein preparation on intake and rumen digestion of a basal corncob diet with urea included at 30 g kg−1. Relative to the control, alfalfa hay decreased (P < 0.05) intake of the basal diet but increased (P < 0.05) total dry matter intake. ALFCW and soybean protein did not affect intake of the basal diet and total dry matter (P > 0.05). C4/C5 VFAs increased intake of basal diet by sheep in one square (P < 0.05) but had no effect (P > 0.05) on intake with sheep in the other square. Alfalfa hay, ALFCW and soybean protein increased (P < 0.05) total VFA concentration in rumen fluid while only alfalfa hay and C4/C5 VFAs consistently increased isobutyrate, isovalerate and valerate levels in rumen fluid (P < 0.05). In comparison with the control diet, soybean protein elevated (P < 0.05) mean rumen ammonia-N levels whereas C4/C5 VFAs depressed them; however, values exceeded 17 mg dL−1 rumen fluid on all treatments. Most measures of digestion kinetics of fiber from corncobs were unaffected by supplementation (P > 0.05). Alfalfa hay and ALFCW increased (P < 0.05) rate of passage of digesta from the reticulo-rumen. It was concluded that alfalfa hay has a reduced role in improving intake of corncob diets when there is also sufficient NPN in the diet. Key words: Roughage (poor quality), volatile fatty acids, rumen ammonia levels, chromium-mordanted cell walls

Control on Soft Rot of Chinese Cabbage Using Harpin Protein

2011 ◽  
Vol 183-185 ◽  
pp. 2078-2081
Author(s):  
Tian Lei Qiu ◽  
Min Wang ◽  
Xiao Hong Sun ◽  
Mei Lin Han ◽  
Xu Ming Wang
Keyword(s):  
Chinese Cabbage ◽  
Soft Rot ◽  
Coli Strain ◽  
Economic Losses ◽  
Common Disease ◽  
Control Effect ◽  
Protein Preparation ◽  
E Coli ◽  
Wettable Powder ◽  
Harpin Protein

Soft rot of Chinese cabbage is a common disease that causes serious damage and economic losses. In this study, the control on soft rot of growing and postharvest Chinese cabbage was carried out, using Harpin protein which was the expressed product of a recombinant E. coli strain. The experimental results indicate that Harpin protein preparation containing 3% pure protein powder and 97% wettable powder of Bacillus Thuringiensis (Bt), could effectively control soft rot of Chinese cabbage. The control effect on soft rot reached as high as 90% for growing Chinese cabbage using Harpin protein at 6-10mg/m2 of dosage, and the control effect reached approximately 75% for postharvest Chinese cabbage. Harpin protein stored for 6 months at 20-25 0C had the similarly biological activity with the newly prepared protein.

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