Determination of cytokine profile and associated genes of the signaling pathway in HNSCC

Abstract This study describes changes in haematological parameters, cytokine profile, histopathology and cortisol levels in Swiss mice experimentally infected with Angiostrongylus costaricensis. Twenty-eight Swiss mice were divided into two groups (G1 and G2) of 14 animals each. In each group, eight animals were infected orally with ten third-stage larvae of A. costaricensis and six were used as a control group. The mice of groups G1 and G2 were sacrificed 14 and 24 days after infection, respectively. Samples were collected for histopathological and haematological analyses and determination of the cytokine profile and cortisol levels. Granulomatous reaction, eosinophilic infiltrate and vasculitis in the intestinal tract, pancreas, liver and spleen were observed with varying intensity in infected animals. Our results showed that the mice developed normocytic and hypochromic anaemia, and that the histopathological lesions caused by the experimental infection influenced increases in cortisol, neutrophil and monocyte levels. In addition to this, we detected increased interleukin-6 and tumour necrosis factor alpha levels in the infected animals.

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Sub-cell FRET imaging for determination of signaling pathway of cell apoptosis during tumor therapy

10.1117/12.741092 ◽

2007 ◽

Author(s):

Lei Liu ◽

Da Xing ◽

Wei R. Chen

Keyword(s):

Signaling Pathway ◽

Cell Apoptosis ◽

Tumor Therapy

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Transcriptosome and Serum Cytokine Profiling of B-CLL Identifies Oncogenic Pathways in Pathogenesis and Survival.

Blood ◽

10.1182/blood.v108.11.4953.4953 ◽

2006 ◽

Vol 108 (11) ◽

pp. 4953-4953

Author(s):

James Choi ◽

Harinder Garewal ◽

Christopher Riley ◽

Laurence Cooke ◽

Thomas Klinkhammer ◽

...

Keyword(s):

Gene Expression ◽

Signaling Pathways ◽

Real Time ◽

Signaling Pathway ◽

Cytokine Profile ◽

Lymphocytic Leukemia ◽

Rt Pcr ◽

Serum Cytokine ◽

Oncogenic Signaling ◽

Oncogenic Signaling Pathways

Abstract Background: B-cell chronic lymphocytic leukemia (B-CLL) the most common leukemia in the Western world accounts for 25% of all newly diagnosed leukemias. Despite new therapeutic advances, B-CLL is currently not curable. Potential oncogenic signaling pathways in B-CLL require elucidation. We identified ROR-1 receptor tyrosine kinase (RTK) in the WNT/Planar Cell Polarity non-canonical pathway as a possible mechanism of oncogenesis by gene expression profiling (GEP). Methods: Mononuclear cells from 8 low stage CLL patients were obtained through an IRB approved protocol and analyzed utilizing the HG-U133A 2.0 Affymetrix array (~18,400 transcripts, 22,000 probe sets) after isolating and purifying total RNA (Qiagen, RNAeasy). The control RNA samples were isolated from 8 normal volunteer peripheral blood (PB) B-cells (AllCell, CA) and a normal reactive lymph node. Tumor lineage was confirmed by immunohistochemistry (IHC). Quantitative real time RT-PCR was performed on 20 selected genes to validate the microarray GEP. Twenty one B-CLL patients (includes the 8 patients from the GEP) were evaluated by RT-PCR for several key components of oncogenic signaling pathways based on the GEP with gene specific probes. A serum cytokine profile (120 cytokines) on 12 CLL patients (8 for which GEP is available and 4 additional patients) and 4 normal volunteers were performed for identification of a signature for diagnostic and prognostic value. Results: Data are represented as “robust” increases or decreases of relative gene expression common to 8 patients. ROR-2, a close member of ROR-1, has been shown to bind Wnt-5A via the cysteine rich domain (CRD) and to activate the JNK signaling pathway. Our findings identify over-expression of members of the non-canonical WNT/PCP- ROR-1- signaling pathway genes that were validated by RT-PCR. A serum cytokine profile of 12 patients provides a signature that may be useful in CLL diagnosis and prognosis. Conclusions: GEP identified WNT/PCP-ROR-1 as key components of an autocrine pathway that helps B-CLL avoid apoptosis. Several serum cytokines are elevated and require validation as potential diagnostic and prognostic markers. GEP of B-CLL in combination with quantitative real time RT-PCR has identified several novel targets for therapy. The identification of ROR-1 RTK has led to the development of a molecular target for future therapeutic application. Several lead compounds have been identified and are being evaluated as potential therapies in B-CLL.

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Determination of the Th1, Th2, Th17, and Treg cytokine profile in patients with chronic Chagas heart disease and systemic arterial hypertension

Heart and Vessels ◽

10.1007/s00380-018-1228-z ◽

2018 ◽

Vol 34 (1) ◽

pp. 123-133 ◽

Cited By ~ 5

Author(s):

Reinaldo B. Bestetti ◽

Renata Dellalibera-Joviliano ◽

Gabriel S. Lopes ◽

Milton Faria-Jr ◽

Rosemary Furlan-Daniel ◽

...

Keyword(s):

Heart Disease ◽

Arterial Hypertension ◽

Cytokine Profile ◽

Systemic Arterial Hypertension ◽

Chagas Heart Disease

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FEATURES OF THE CYTOKINE PROFILE IN INFANTS WITH MULTIPLE FOOD PROTEIN INTOLERANCE

Rossiiskii Allergologicheskii ZHurnal ◽

10.36691/rja731 ◽

2012 ◽

Vol 9 (5) ◽

pp. 76-80

Author(s):

E E Varlamov ◽

T V Vinogradova ◽

A A Chuslyaeva ◽

A N Pampura

Keyword(s):

Atopic Dermatitis ◽

Food Allergy ◽

Amino Acid ◽

Inflammatory Process ◽

Allergic Diseases ◽

Cytokine Profile ◽

Food Protein ◽

Additional Indication ◽

Protein Intolerance

Introduction. Types of the course of allergic diseases may be related to cytokine profile. Determination of the spectrum of cytokines to evaluate the mechanisms of allergic inflammatory process that improves the efficiency of management of patients with allergic diseases was performed. Background. To establish the features of cytokine profile in children with multiple food protein intolerance. Methods. 39 children with atopic dermatitis and food allergy were included in the openlabel study, 18 of whom had multiple food protein intolerance. All patients were examined with enzyme immunoassay (ELISA) to determine the concentration of IL-4, IL-5, IL-13, IL-17, IL-22, eotaxin, eotaxin2, TGF-β. Results. In children with multiple food protein intolerance the concentration of TGF-β was increased in comparison with the group of children without multiple food intolerances Me 14,04 [11,3; 18,0] against 10,3 [4,8; 12, 8] pg/ml, respectively, p = 0,038. The level of other cytokines had no difference. Conclusion. Increased concentration of TGF-β in patients with multiple food protein intolerance may be an additional indication for the amino acid formulas.

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CYTOKINE PROFILE AND IMPORTANCE OF ITS ASSESSMENT IN FORECASTING THE COURSE OF DUST DISEASES OF THE LUNGS

Science and Innovations in Medicine ◽

10.35693/2500-1388-2017-0-1-61-64 ◽

2017 ◽

Vol 2 (1) ◽

pp. 61-64

Author(s):

SA A Babanov ◽

DS S Budash

Keyword(s):

Lung Diseases ◽

Secondary Prophylaxis ◽

Cytokine Profile ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Complete Physical Examination ◽

Occupational Lung Diseases

Aim - study of cellular immunity and cytokine profile in dust lung diseases caused by exposure to aerosols of various degrees of fibrogenity; assessment of the role of immunological changes in the pathogenesis of occupational lung diseases of dust aetiology. Materials and methods. 161 patients from the contact group with chronic dust bronchitis, silicosis and pneumoconiosis due to exposure to welding aerosols were examined. The control group included 60 people who did not have contact with occupational hazards at work and who were declared healthy according to complete physical examination. The diagnosis was made on the basis of professional anamnesis, sanitary-hygienic characteristics of working conditions (work in contact with aerosols of various degrees of fibrogenity), and a complete clinical and instrumental examination of patients. The levels of cytokines IL-1a, IL-ф, IL-4, IL-8, TNF-a, IFN-y and fibronectin in the blood serum were determined using enzyme-linked immunosorbent assay. Results. The specific features of the course of dust diseases of the lungs were revealed (identified features of the cytokine profile, hypercytokinaemia of a significant number of cytokines), which allows us to characterize the occurrence and the progression of dust diseases of the lungs. Conclusion. Determination of the cytokine profile allows us not only to improve the quality of early detection, but also to optimize the strategy of primary and secondary prophylaxis of this disease, to predict the course of the disease, and to reduce the number of disabling forms.

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Comparative analysis of diagnostic significance of determination of antimicrobial and cytokine profile of rotal and design joint bone in pregnant women with chronic generalized parodontite

Rossiiskaya stomatologiya ◽

10.17116/rosstomat20181104128 ◽

2018 ◽

Vol 11 (4) ◽

pp. 28

Author(s):

S. O. Surmeneva

Keyword(s):

Comparative Analysis ◽

Pregnant Women ◽

Cytokine Profile ◽

Diagnostic Significance

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DETERMINING OF CYTOKINE LEVELS IN THE URINE IN CLINICAL PRACTICE

Russian Clinical Laboratory Diagnostics ◽

10.18821/0869-2084-2019-64-5-287-293 ◽

2019 ◽

Vol 64 (5) ◽

pp. 287-293

Author(s):

L. M. Karzakova ◽

S. I. Kudryashov ◽

M. V. Shestipalova ◽

E. V. Leontyeva

Keyword(s):

Cytokine Profile ◽

Renal Pathology ◽

Acute Glomerulonephritis ◽

Healthy Individuals ◽

Tnf Α ◽

Cytokine Levels ◽

N 93 ◽

High Level ◽

Observation Period

The paper presents data on the study of the content of cytokines (IL-1β, RAIL-1β, IL-2, IL-4, IL-10, IL-17A, TNF-, IFN-γ) in the morning urine using enzyme immunoassay in healthy individuals (n = 20) and in patients with acute glomerulonephritis (n = 93). The determination of cytokine levels in patients was carried out in the debut of the disease and 12 months after the onset of the disease. The obtained indicators of cytokine content in the urine are presented as absolute values in pg/ml and creatinine-normalized values calculated by the formula: cytokine level (pg/ml) / urine creatinine (µmol/ml). The study was made of changes in the content of cytokines in the urine of patients with glomerulonephritis with respect to a group of healthy individuals, as well as the dynamics of the content of cytokines in the urine during the 12-month observation period. The results of the study showed that the absolute values of cytokines in urine can distort the true picture of the cytokine profile of urine in renal pathology. Normalized values of the predominant number of pro- and anti-inflammatory cytokines (IL-1β, IL-2, IL-8, IL-10, IL-17A and TNF-α) in patients with glomerulonephritis were significantly higher than the corresponding indicators of healthy individuals. The normalized values of cytokines were shown to be as more sensitive indicators than absolute values in the course of analyzing differences in the cytokine profile in patients with glomerulonephritis, depending on chronic and acute course of the disease. These indicators influenced the outcome of glomerulonephritis, assessed, as a rule, 12 months after the onset of the disease. Thus, the low levels of IL-1β, IL-8 and IL-17А detected in the debut of the disease in combination with the high level of RAIL-1β determined the chronization of glomerulonephritis. So, the creatinine-normalized cytokine levels in the urine expand the possibilities of using the evaluation of the cytokine profile of urine to establish changes in the cytokine content in the urine in renal pathology and predict the chronization of glomerulonephritis.

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