Improved transdermal permeability of tanshinone IIA from cataplasms by loading onto nanocrystals and porous silica

Background: The purpose of this study was to determine whether myeloid differentiation factor88-dependent Toll-Like Receptor-4 (TLR-4) signaling contributed to the inhibition of abdominal aortic aneurysm (AAA) by Tanshinone IIA (Tan IIA). Materials and methods: Male Sprague-Dawley rats (n = 12 / group) were randomly distributed into three groups: Tan IIA, control, and sham. The rats from Tan IIA and control groups under-went intra-aortic elastase perfusion to induce AAAs, and those in the sham group were perfused with saline. Only the Tan IIA group received Tan IIA (2 mg / rat / d). Aortic tissue samples were harvested at 24 d after perfusion and evaluated using reverse transcriptase-polymerase chain reaction, Western blot, immunohistochemistry and immunofluorescence. Results: The over-expression of Toll-Like Receptor-4 (TLR-4), Myeloid Differentiation factor 88 (MyD88), Phosphorylated Nuclear Factor κB (pNF-κB) and Phosphorylated IκBα (pIκBα) induced by elastase perfusion were significantly decreased by Tan IIA treatment. Conclusions: Tan IIA attenuates elastase-induced AAA in rats possibly via the inhibition of MyD88-dependent TLR-4 signaling, which may be one potential explanation of why Tan IIA inhibits AAA development through multiple effects.

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SiO2 Entrapment of Animal Cells for Hybrid Bioartificial Organs

MRS Proceedings ◽

10.1557/proc-628-cc10.1 ◽

2000 ◽

Vol 628 ◽

Cited By ~ 6

Author(s):

Giovanni Carturan ◽

Renzo Dal Monte ◽

Maurizio Muraca

Keyword(s):

Gas Phase ◽

Mechanical Stability ◽

Porous Silica ◽

Collagen Matrix ◽

Homogeneous Layer ◽

Animal Cells ◽

Bioartificial Organs ◽

Liver And Pancreas ◽

Encapsulation Method ◽

Metabolic Activities

ABSTRACTSi-alkoxides in gas phase are reactive towards the surface of animal cells, depositing a homogeneous layer of porous silica. This encapsulation method preserves cell viability and does not alter the hindrance of the biological load.In the prospective use for the design of a hybrid bioartificial liver, hepatocytes in a collagen matrix can be entrapped by the siliceous deposit which provides definite mechanical stability to the collagen matrix and molecular cutoff vs. high molecular weight proteins, including immunoglobulins. The functionality of the encapsulated cell load is maintained for the expressions of typical liver and pancreas metabolic activities.

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Preparation and Thermal Stability of Porous Silica-based Monoliths

Journal of Inorganic Materials ◽

10.3724/sp.j.1077.2011.00949 ◽

2011 ◽

Vol 26 (9) ◽

pp. 949-954

Author(s):

Li-Jie ZHANG ◽

Jing-Hua GU ◽

Hong-Ying YAO ◽

Yue ZHANG

Keyword(s):

Thermal Stability ◽

Porous Silica ◽

Thermal Stability Of

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Tanshinone IIA Promotes Macrophage Cholesterol Efflux and Attenuates Atherosclerosis of apoE-/- Mice by Omentin-1/ABCA1 Pathway

Current Pharmaceutical Biotechnology ◽

10.2174/1389201020666190404125213 ◽

2019 ◽

Vol 20 (5) ◽

pp. 422-432 ◽

Cited By ~ 3

Author(s):

Yu-lin Tan ◽

Han-xiao Ou ◽

Min Zhang ◽

Duo Gong ◽

Zhen-wang Zhao ◽

...

Keyword(s):

Atherosclerotic Plaque ◽

Lipid Content ◽

Plasma Levels ◽

Cholesterol Efflux ◽

Density Lipoprotein ◽

Tanshinone Iia ◽

Lipoprotein Cholesterol ◽

Mrna Levels ◽

Cellular Lipid ◽

Oil Red O

Background: Tanshinone IIA (Tan IIA) and Omentin-1 have a protective role in the cardiovascular system. However, if and how Tan IIA and Omentin-1 regulate cholesterol metabolism in macrophages has not been fully elucidated. Objective: To investigate the possible mechanisms of Tan IIA and Omentin-1 on preventing macrophage cholesterol accumulation and atherosclerosis development. Methods: The effect of Tan IIA on the protein and mRNA levels of Omentin-1 and ATP-binding cassette transporter A1 (ABCA1) in macrophages was examined by Western blot and qRT-PCR assay, respectively. Cholesterol efflux was assessed by liquid scintillation counting (LSC). Cellular lipid droplet was measured by Oil Red O staining, and intracellular lipid content was detected by high performance liquid chromatography (HPLC). In addition, the serum lipid profile of apoE−/− mice was measured by enzymatic method. The size of atherosclerotic lesion areas and content of lipids and collagen in the aortic of apoE−/− mice were examined by Sudan IV, Oil-red O, and Masson staining, respectively. Results: Tan IIA up-regulated expression of Omentin-1 and ABCA1 in THP-1 macrophages, promoting ABCA1-mediated cholesterol efflux and consequently decreasing cellular lipid content. Consistently, Tan IIA increased reverse cholesterol transport in apoE−/− mice. Plasma levels of high-density lipoprotein cholesterol (HDL-C), ABCA1 expression and atherosclerotic plaque collagen content were increased while plasma levels of low-density lipoprotein cholesterol (LDL-C) and atherosclerotic plaque sizes were reduced in Tan IIA-treated apoE−/− mice. These beneficial effects were, however, essentially blocked by knockdown of Omentin-1. Conclusion: Our results revealed that Tan IIA promotes cholesterol efflux and ameliorates lipid accumulation in macrophages most likely via the Omentin-1/ABCA1 pathway, reducing the development of aortic atherosclerosis.

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Simultaneous Determination of Six Components in Jingzhiguanxin Tablet by High-Performance Liquid Chromatography

Current Pharmaceutical Analysis ◽

10.2174/1573412914666180315120130 ◽

2019 ◽

Vol 15 (2) ◽

pp. 130-137

Author(s):

Hui Jiang ◽

Lianhao Fu ◽

Yu Wang ◽

Shaozhi Wang ◽

Xiaoxu Zhang ◽

...

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

High Performance ◽

Gradient Elution ◽

Tanshinone Iia ◽

Array Detector ◽

Control Analysis ◽

Active Components ◽

Quality Control Analysis

Background: Jingzhiguanxin (JZGX) tablet, a traditional Chinese prescription, is commonly used for treating coronary heart disease and angina pectoris in the clinic. There are six active components (Danshensu (DSS), Protocatechuic aldehyde (PD), Paeoniflorin (PF), Ferulic acid (FA), Salvianolic acid B (Sal B) and Tanshinone IIA (TA)) in JZGX tablet. </P><P> Objective: In this paper, a simple and reliable method was used for simultaneous determining the six active components by high-performance liquid chromatography coupled with diode array detector (HPLC-DAD). Methods: These six active components were separated on an Agilent Zorbax Eclipse XDB-C18 column (150 mmx4.6 mm, 5 µm) at 30 °C. Acetonitrile (A), methanol (B) and 0.5% H3PO4 aqueous solution (C) were used as mobile phase for gradient elution. The flow rate was 1 mL/min and the detection wavelengths were set at 280 nm for DSS, PD and Sal B, 230 nm for PF, 320 nm for FA and 270 nm for TA, respectively. Results: All of the six components showed good linearity regressions (r2≥0.9997) in the detected concentration range. The recovery rates and coefficient of variation (CV) for all analytes were 98.66%- 100.18% and 0.75%-1.89%, respectively. This method was successfully applied to simultaneously determine the six components in JZGX tablet from different batches and manufacturers. Conclusion: The validated method can be used in routine quality control analysis of JZGX tablet without any interference.

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